Type IV secretion system protein virB8 - Brucella suis biovar 1 (strain 1330)

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Q7CEG3 (VIRB8_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 66. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Type IV secretion system protein virB8

Gene names

Name:	virB8
Ordered Locus Names:	BRA0062, BS1330_II0062

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella ›

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The VirB system could be required for the establishment of the replication niche in the host.
Subcellular location	Cell inner membrane; Single-pass membrane protein Potential.
Induction	Specifically induced within macrophages by phagosome acidification. Induced at 37 degrees Celsius in minimal medium, suggesting that nutritional stress is a regulating signal.
Miscellaneous	Transcription of the operon is maximal in early exponential phase.
Sequence similarities	Belongs to the virB8 family.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW protein secretion by the type IV secretion system Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

239

Type IV secretion system protein virB8

PRO_0000291392

Regions

Transmembrane

21

Helical; Potential

Secondary structure

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239

Helix Strand Turn

Sequences

Sequence

Length

Mass (Da)

Tools

Q7CEG3 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 68F44CBCF7018023
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239

26,446

        10         20         30         40         50         60 
MFGRKQSPQK SVKNGQGNAP SVYDEALNWE AAHVRLVEKS ERRAWKIAGA FGTITVLLGI 

        70         80         90        100        110        120 
GIAGMLPLKQ HVPYLVRVNA QTGAPDILTS LDEKSVSYDT VMDKYWLSQY VIARETYDWY 

       130        140        150        160        170        180 
TLQKDYETVG MLSSPSEGQS YASQFQGDKA LDKQYGSNVR TSVTIVSIVP NGKGIGTVRF 

       190        200        210        220        230 
AKTTKRTNET GDGETTHWIA TIGYQYVNPS LMSESARLTN PLGFNVTSYR VDPEMGVVQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis Ptl type IV secretion systems is essential for intracellular survival of Brucella suis." O'Callaghan D., Cazevieille C., Allardet-Servent A., Boschiroli M.L., Bourg G., Foulongne V., Frutos P., Kulakov Y., Ramuz M. Mol. Microbiol. 33:1210-1220(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1330.
[2]	"The Brucella suis virB operon is induced intracellularly in macrophages." Boschiroli M.L., Ouahrani-Bettache S., Foulongne V., Michaux-Charachon S., Bourg G., Allardet-Servent A., Cazevieille C., Liautard J.P., Ramuz M., O'Callaghan D. Proc. Natl. Acad. Sci. U.S.A. 99:1544-1549(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], EXPRESSION CONDITIONS. Strain: 1330.
[3]	"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
[4]	"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
[5]	"Structures of two core subunits of the bacterial type IV secretion system, VirB8 from Brucella suis and ComB10 from Helicobacter pylori." Terradot L., Bayliss R., Oomen C., Leonard G.A., Baron C., Waksman G. Proc. Natl. Acad. Sci. U.S.A. 102:4596-4601(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 77-239.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF141604 Genomic DNA. Translation: AAD56618.1.
AE014292 Genomic DNA. Translation: AAN33274.1.
CP002998 Genomic DNA. Translation: AEM19554.1.

RefSeq

NP_699269.1. NC_004311.2.
YP_005613780.1. NC_017250.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BHM	X-ray	2.40	A/B/C/D/E	77-239	[»]
4AKY	X-ray	2.60	A/B/C/D/E	97-234	[»]
4AKZ	X-ray	2.25	A/B/C/D/E	97-234	[»]

ProteinModelPortal

SMR

Q7CEG3. Positions 97-234.

ModBase

Protein-protein interaction databases

STRING

204722.BRA0062.

Protocols and materials databases

StructuralBiologyKnowledgebase

Genome annotation databases

EnsemblBacteria

AAN33274; AAN33274; BRA0062.
AEM19554; AEM19554; BS1330_II0062.

GeneID

1164499.
12139496.

KEGG

bms:BRA0062.
bsi:BS1330_II0062.

PATRIC

17792927. VBIBruSui107850_2282.

Organism-specific databases

CMR

Phylogenomic databases

eggNOG

HOGENOM

KO

OMA

ProtClustDB

Family and domain databases

InterPro

IPR007430. VirB8.
IPR026264. VirB8/PtlE.
[Graphical view]

Pfam

PF04335. VirB8. 1 hit.
[Graphical view]

PIRSF

PIRSF003299. VirB8_PtlE. 1 hit.

ProtoNet

Other

EvolutionaryTrace

Entry information

Entry name

VIRB8_BRUSU

Accession

Primary (citable) accession number: Q7CEG3
Secondary accession number(s): G0KER0, Q7BQL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Brucella suis (strain 1330): entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents