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Q7CEG3 (VIRB8_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type IV secretion system protein virB8
Gene names
Name:virB8
Ordered Locus Names:BRA0062, BS1330_II0062
OrganismBrucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]
Taxonomic identifier204722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length239 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The VirB system could be required for the establishment of the replication niche in the host.

Subcellular location

Cell inner membrane; Single-pass membrane protein Potential.

Induction

Specifically induced within macrophages by phagosome acidification. Induced at 37 degrees Celsius in minimal medium, suggesting that nutritional stress is a regulating signal.

Miscellaneous

Transcription of the operon is maximal in early exponential phase.

Sequence similarities

Belongs to the virB8 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself3EBI-6407073,EBI-6407073

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 239239Type IV secretion system protein virB8
PRO_0000291392

Regions

Transmembrane47 – 6721Helical; Potential

Secondary structure

........................ 239
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q7CEG3 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 68F44CBCF7018023

FASTA23926,446
        10         20         30         40         50         60 
MFGRKQSPQK SVKNGQGNAP SVYDEALNWE AAHVRLVEKS ERRAWKIAGA FGTITVLLGI 

        70         80         90        100        110        120 
GIAGMLPLKQ HVPYLVRVNA QTGAPDILTS LDEKSVSYDT VMDKYWLSQY VIARETYDWY 

       130        140        150        160        170        180 
TLQKDYETVG MLSSPSEGQS YASQFQGDKA LDKQYGSNVR TSVTIVSIVP NGKGIGTVRF 

       190        200        210        220        230 
AKTTKRTNET GDGETTHWIA TIGYQYVNPS LMSESARLTN PLGFNVTSYR VDPEMGVVQ 

« Hide

References

« Hide 'large scale' references
[1]"A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis Ptl type IV secretion systems is essential for intracellular survival of Brucella suis."
O'Callaghan D., Cazevieille C., Allardet-Servent A., Boschiroli M.L., Bourg G., Foulongne V., Frutos P., Kulakov Y., Ramuz M.
Mol. Microbiol. 33:1210-1220(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 1330.
[2]"The Brucella suis virB operon is induced intracellularly in macrophages."
Boschiroli M.L., Ouahrani-Bettache S., Foulongne V., Michaux-Charachon S., Bourg G., Allardet-Servent A., Cazevieille C., Liautard J.P., Ramuz M., O'Callaghan D.
Proc. Natl. Acad. Sci. U.S.A. 99:1544-1549(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], EXPRESSION CONDITIONS.
Strain: 1330.
[3]"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts."
Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. expand/collapse author list , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[4]"Revised genome sequence of Brucella suis 1330."
Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.
J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[5]"Structures of two core subunits of the bacterial type IV secretion system, VirB8 from Brucella suis and ComB10 from Helicobacter pylori."
Terradot L., Bayliss R., Oomen C., Leonard G.A., Baron C., Waksman G.
Proc. Natl. Acad. Sci. U.S.A. 102:4596-4601(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 77-239.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF141604 Genomic DNA. Translation: AAD56618.1.
AE014292 Genomic DNA. Translation: AAN33274.1.
CP002998 Genomic DNA. Translation: AEM19554.1.
RefSeqNP_699269.1. NC_004311.2.
YP_005613780.1. NC_017250.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2BHMX-ray2.40A/B/C/D/E77-239[»]
4AKYX-ray2.60A/B/C/D/E97-234[»]
4AKZX-ray2.25A/B/C/D/E97-234[»]
ProteinModelPortalQ7CEG3.
SMRQ7CEG3. Positions 97-234.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ7CEG3. 1 interaction.
MINTMINT-8194075.
STRING204722.BRA0062.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN33274; AAN33274; BRA0062.
AEM19554; AEM19554; BS1330_II0062.
GeneID1164499.
12139496.
KEGGbms:BRA0062.
bsi:BS1330_II0062.
PATRIC17792927. VBIBruSui107850_2282.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3736.
HOGENOMHOG000119601.
KOK03203.
OMAQDKHWVE.
OrthoDBEOG6Z9B0F.
ProtClustDBCLSK884263.

Family and domain databases

InterProIPR007430. VirB8.
IPR026264. VirB8/PtlE.
[Graphical view]
PfamPF04335. VirB8. 1 hit.
[Graphical view]
PIRSFPIRSF003299. VirB8_PtlE. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ7CEG3.
PROQ7CEG3.

Entry information

Entry nameVIRB8_BRUSU
AccessionPrimary (citable) accession number: Q7CEG3
Secondary accession number(s): G0KER0, Q7BQL4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Brucella suis

Brucella suis (strain 1330): entries and gene names