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Q7CEG3

- VIRB8_BRUSU

UniProt

Q7CEG3 - VIRB8_BRUSU

Protein

Type IV secretion system protein virB8

Gene

virB8

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    The VirB system could be required for the establishment of the replication niche in the host.

    GO - Molecular functioni

    1. identical protein binding Source: IntAct

    GO - Biological processi

    1. pathogenesis Source: UniProtKB-KW
    2. protein secretion by the type IV secretion system Source: InterPro

    Keywords - Biological processi

    Virulence

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type IV secretion system protein virB8
    Gene namesi
    Name:virB8
    Ordered Locus Names:BRA0062, BS1330_II0062
    OrganismiBrucella suis biovar 1 (strain 1330)
    Taxonomic identifieri204722 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000824: Chromosome II, UP000007104: Chromosome II

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 239239Type IV secretion system protein virB8PRO_0000291392Add
    BLAST

    Expressioni

    Inductioni

    Specifically induced within macrophages by phagosome acidification. Induced at 37 degrees Celsius in minimal medium, suggesting that nutritional stress is a regulating signal.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself3EBI-6407073,EBI-6407073

    Protein-protein interaction databases

    IntActiQ7CEG3. 1 interaction.
    MINTiMINT-8194075.
    STRINGi204722.BRA0062.

    Structurei

    Secondary structure

    1
    239
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi98 – 11518
    Helixi119 – 1213
    Helixi122 – 13211
    Helixi135 – 1428
    Helixi143 – 1453
    Beta strandi146 – 1494
    Helixi151 – 1555
    Beta strandi158 – 17013
    Beta strandi172 – 18918
    Beta strandi194 – 20613
    Helixi209 – 2113
    Helixi214 – 2174
    Beta strandi224 – 23310

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2BHMX-ray2.40A/B/C/D/E77-239[»]
    4AKYX-ray2.60A/B/C/D/E97-234[»]
    4AKZX-ray2.25A/B/C/D/E97-234[»]
    ProteinModelPortaliQ7CEG3.
    SMRiQ7CEG3. Positions 97-234.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ7CEG3.

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei47 – 6721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the virB8 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG3736.
    HOGENOMiHOG000119601.
    KOiK03203.
    OMAiEMSDESV.
    OrthoDBiEOG6Z9B0F.

    Family and domain databases

    InterProiIPR007430. VirB8.
    IPR026264. VirB8/PtlE.
    [Graphical view]
    PfamiPF04335. VirB8. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003299. VirB8_PtlE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q7CEG3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFGRKQSPQK SVKNGQGNAP SVYDEALNWE AAHVRLVEKS ERRAWKIAGA    50
    FGTITVLLGI GIAGMLPLKQ HVPYLVRVNA QTGAPDILTS LDEKSVSYDT 100
    VMDKYWLSQY VIARETYDWY TLQKDYETVG MLSSPSEGQS YASQFQGDKA 150
    LDKQYGSNVR TSVTIVSIVP NGKGIGTVRF AKTTKRTNET GDGETTHWIA 200
    TIGYQYVNPS LMSESARLTN PLGFNVTSYR VDPEMGVVQ 239
    Length:239
    Mass (Da):26,446
    Last modified:July 5, 2004 - v1
    Checksum:i68F44CBCF7018023
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF141604 Genomic DNA. Translation: AAD56618.1.
    AE014292 Genomic DNA. Translation: AAN33274.1.
    CP002998 Genomic DNA. Translation: AEM19554.1.
    RefSeqiNP_699269.1. NC_004311.2.
    YP_005613780.1. NC_017250.1.

    Genome annotation databases

    EnsemblBacteriaiAAN33274; AAN33274; BRA0062.
    AEM19554; AEM19554; BS1330_II0062.
    GeneIDi1164499.
    12139496.
    KEGGibms:BRA0062.
    bsi:BS1330_II0062.
    PATRICi17792927. VBIBruSui107850_2282.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF141604 Genomic DNA. Translation: AAD56618.1 .
    AE014292 Genomic DNA. Translation: AAN33274.1 .
    CP002998 Genomic DNA. Translation: AEM19554.1 .
    RefSeqi NP_699269.1. NC_004311.2.
    YP_005613780.1. NC_017250.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2BHM X-ray 2.40 A/B/C/D/E 77-239 [» ]
    4AKY X-ray 2.60 A/B/C/D/E 97-234 [» ]
    4AKZ X-ray 2.25 A/B/C/D/E 97-234 [» ]
    ProteinModelPortali Q7CEG3.
    SMRi Q7CEG3. Positions 97-234.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q7CEG3. 1 interaction.
    MINTi MINT-8194075.
    STRINGi 204722.BRA0062.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN33274 ; AAN33274 ; BRA0062 .
    AEM19554 ; AEM19554 ; BS1330_II0062 .
    GeneIDi 1164499.
    12139496.
    KEGGi bms:BRA0062.
    bsi:BS1330_II0062.
    PATRICi 17792927. VBIBruSui107850_2282.

    Phylogenomic databases

    eggNOGi COG3736.
    HOGENOMi HOG000119601.
    KOi K03203.
    OMAi EMSDESV.
    OrthoDBi EOG6Z9B0F.

    Miscellaneous databases

    EvolutionaryTracei Q7CEG3.
    PROi Q7CEG3.

    Family and domain databases

    InterProi IPR007430. VirB8.
    IPR026264. VirB8/PtlE.
    [Graphical view ]
    Pfami PF04335. VirB8. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003299. VirB8_PtlE. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis Ptl type IV secretion systems is essential for intracellular survival of Brucella suis."
      O'Callaghan D., Cazevieille C., Allardet-Servent A., Boschiroli M.L., Bourg G., Foulongne V., Frutos P., Kulakov Y., Ramuz M.
      Mol. Microbiol. 33:1210-1220(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 1330.
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], EXPRESSION CONDITIONS.
      Strain: 1330.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1330.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1330.
    5. "Structures of two core subunits of the bacterial type IV secretion system, VirB8 from Brucella suis and ComB10 from Helicobacter pylori."
      Terradot L., Bayliss R., Oomen C., Leonard G.A., Baron C., Waksman G.
      Proc. Natl. Acad. Sci. U.S.A. 102:4596-4601(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 77-239.

    Entry informationi

    Entry nameiVIRB8_BRUSU
    AccessioniPrimary (citable) accession number: Q7CEG3
    Secondary accession number(s): G0KER0, Q7BQL4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 12, 2007
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Transcription of the operon is maximal in early exponential phase.

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. Brucella suis
      Brucella suis (strain 1330): entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3