Q7BUF8 (Q7BUF8_AMYMD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Aminodehydroquinate dehydratase EMBL AAS07762.1

Gene names

Name:

rifJ EMBL AAS07762.1

Organism

Amycolatopsis mediterranei (Nocardia mediterranei) EMBL AAS07762.1

Taxonomic identifier

33910 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Amycolatopsis

Protein attributes

Sequence length	163 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity

3-dehydroquinate = 3-dehydroshikimate + H2O. SAAS SAAS018509

Ontologies

Keywords
Molecular function	Lyase SAAS SAAS018509
Gene Ontology (GO)
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	35	1	Proton acceptor By similarity PIRSR PIRSR001399-1
	Active site	112	1	Proton donor By similarity PIRSR PIRSR001399-1
	Site	30	1	Transition state stabilizer By similarity PIRSR PIRSR001399-5

Sequences

	Sequence		Length	Mass (Da)
	Q7BUF8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 45990D24E8E39019	FASTA	163	17,597
	10 20 30 40 50 60 MVQAPCRTRC VPLPDVLLLN GPNLGVLGRR EPEIYGTDTL ADIEKAVAEE VRPRGWTVVP 70 80 90 100 110 120 VQRDGEGELV GAIHQHRDTT VGAIVNPGAL MIAGWSLRDA LASYEPPWIE VHLSNVWARE 130 140 150 160 QFRHDSVIAP LASGVVVGLG AFGYRLAAQA LLHLSAESPN GLR

References

[1]	"Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699." August P.R., Tang L., Yoon Y.J., Ning S., Muller R., Yu T.W., Taylor M., Hoffmann D., Kim C.G., Zhang X., Hutchinson C.R., Floss H.G. Chem. Biol. 5:69-79(1998) [PubMed: 9512878] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AAS07762.1.
[2]	"3-amino-5-hydroxybenzoic acid synthase, the terminal enzyme in the formation of the precursor of mC7N units in rifamycin and related antibiotics." Kim C.G., Yu T.W., Fryhle C.B., Handa S., Floss H.G. J. Biol. Chem. 273:6030-6040(1998) [PubMed: 9497318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AAS07762.1.
[3]	"Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei S699." Yu T.W., Muller R., Muller M., Zhang X., Draeger G., Kim C.G., Leistner E., Floss H.G. J. Biol. Chem. 276:12546-12555(2001) [PubMed: 11278540] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AAS07762.1.
[4]	"Rifamycin insusceptibility: exploring the rif gene cluster of Amycolatopsis mediterranei S699." Yu T.-W., Pogosova-Agadjanyan E.L., Kuan L.-Y., Bai L., Tin A.M., Adman E., Floss H.G. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AAS07762.1.
[5]	Yu T., August P.R., Tang L., Yoon Y.J., Ning S., Mueller R., Taylor M., Kim C., Zhang X., Pogosova-Agadjanyan E.L., Tin A.M., Hutchinson C.R., Floss H.G. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AAS07762.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF040570 Genomic DNA. Translation: AAS07762.1.
3D structure databases
ProteinModelPortal	Q7BUF8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00169. AroQ. [Tree]
InterPro	IPR001874. DHquinase_II. IPR018509. DHquinase_II_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.9100. DHquinase_II. 1 hit.
PANTHER	PTHR21272. DHquinase_II. 1 hit.
Pfam	PF01220. DHquinase_II. 1 hit. [Graphical view]
PIRSF	PIRSF001399. DHquinase_II. 1 hit.
ProDom	PD004527. DHquinase_II. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF52304. DHquinase_II. 1 hit.
PROSITE	PS01029. DEHYDROQUINASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q7BUF8_AMYMD

Accession

Primary (citable) accession number: Q7BUF8

Entry history

Integrated into UniProtKB/TrEMBL:	July 5, 2004
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)