Q7BSX4 (YFI3_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized endonuclease
EC=3.1.-.-

Gene names

Ordered Locus Names:

L7003, ECO57PM65

Encoded on

Plasmid pO157

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Sequence similarities

Belongs to the thermonuclease family.

Ontologies

Keywords
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Molecular function	endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 153	134	Uncharacterized endonuclease		PRO_0000034396
Sites
	Active site	46	1	By similarity
	Active site	54	1	By similarity
	Active site	88	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7BSX4 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 635483EEA027D090	FASTA	153	17,872
	10 20 30 40 50 60 MRKYIPLVLF IFSWPVLCAD IHGRVVRVLD GDTIEVMDSR KAVRIRLVNI DAPEKKQDYG 70 80 90 100 110 120 RWSTDMMKSL VAGKTVTVTY FQRDRYGRML GQVYAPDGMN VNQFMVRAGA AWVYEQYNTD 130 140 150 PVLPVLQNEA RQQKRGLWSD ADPVPPWIWR HRK

References

[1]

"The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7." Burland V., Shao Y., Perna N.T., Plunkett G. III, Sofia H.J., Blattner F.R. Nucleic Acids Res. 26:4196-4204(1998) [PubMed: 9722640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete nucleotide sequences of 93-kb and 3.3-kb plasmids of an enterohemorrhagic Escherichia coli O157:H7 derived from Sakai outbreak." Makino K., Ishii K., Yasunaga T., Hattori M., Yokoyama K., Yatsudo H.C., Kubota Y., Yamaichi Y., Iida T., Yamamoto K., Honda T., Han C.G., Ohtsubo A., Kasamatsu M., Hayashi T., Kuhara S., Shinagawa H. DNA Res. 5:1-9(1998) [PubMed: 9628576] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF074613 Genomic DNA. Translation: AAC70071.1. AB011549 Genomic DNA. Translation: BAA31822.1.
PIR	T00303.
RefSeq	NP_052672.1. NC_002128.1. YP_325563.1. NC_007414.1.
3D structure databases
ProteinModelPortal	Q7BSX4.
SMR	Q7BSX4. Positions 22-139.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000029386; EBESCP00000028091; EBESCG00000028436. EBESCT00000060730; EBESCP00000058374; EBESCG00000059777.
GeneID	1789676. 3683670.
GenomeReviews	Gene locus ECO57PM65 in contig AB011549_GR. Gene locus L7003 in contig AF074613_GR.
KEGG	ece:L7003.
PATRIC	18360387. VBIEscCol44059_5595.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000008797.
HOGENOM	HBG752546.
OMA	IEPYKWR.
ProtClustDB	CLSK861833.
Family and domain databases
InterPro	IPR016071. Staphylococal_nuclease_OB-fold. IPR006021. Staphylococcal_nuclease. IPR002071. Thermonucl_AS. [Graphical view]
Gene3D	G3DSA:2.40.50.90. SNase. 1 hit.
Pfam	PF00565. SNase. 1 hit. [Graphical view]
SMART	SM00318. SNc. 1 hit. [Graphical view]
SUPFAM	SSF50199. Staphylococal_nuclease_OB-fold. 1 hit.
PROSITE	PS01123. TNASE_1. 1 hit. PS01284. TNASE_2. 1 hit. PS50830. TNASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YFI3_ECO57

Accession

Primary (citable) accession number: Q7BSX4
Secondary accession number(s): O82923

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program