Q7BSW8 (KATG2_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase 2
Short name=CP 2
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase 2

Gene names

Name:	katG2
Synonyms:	katP
Ordered Locus Names:	L7017, ECO57PM75

Encoded on

Plasmid pO157 Ref.1 Ref.2 Ref.3

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	736 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity. Ref.1
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Subcellular location	Periplasm Ref.1.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Cellular component	Periplasm
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 736	713	Catalase-peroxidase 2 HAMAP MF_01961		PRO_0000354782
Sites
	Active site	103	1	Proton acceptor By similarity
	Metal binding	264	1	Iron (heme axial ligand) By similarity
	Site	99	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	102 ↔ 223		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-249) By similarity
	Cross-link	223 ↔ 249		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-102) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7BSW8 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: F3EA593D3FDEDB8D	FASTA	736	81,794
	10 20 30 40 50 60 MIKKTLPVLI LLALSGSFST AVAADKKETQ NFYYPETLDL TPLRLHSPES NPWGADFDYA 70 80 90 100 110 120 TRFQQLDMEA LKKDIKDLLT TSQDWWPADY GHYGPFFIRM AWHGAGTYRT YDGRGGASGG 130 140 150 160 170 180 QQRFEPLNSW PDNVNLDKAR RLLWPVKKKY GSSISWGDLM VLTGNVALES MGFKTLGFAG 190 200 210 220 230 240 GREDDWESDL VYWGPDNKPL ADNRDKNGKL QKPLAATQMG LIYVNPEGPG GKPDPLASAK 250 260 270 280 290 300 DIREAFSRMA MDDEETVALI AGGHTFGKAH GAASPEKCIG AGPDGAPVEE QGLGWKNKCG 310 320 330 340 350 360 TGNGKYTITS GLEGAWSTSP TQFTMQYLKN LYKYEWELHK SPAGAYQWKP KKAANIVQDA 370 380 390 400 410 420 HDPSVLHPLM MFTTDIALKV DPEYKKITTR FLNDPKAFEQ AFARAWFKLT HRDMGPAARY 430 440 450 460 470 480 LGNEVPAESF IWQDPLPAAD YTMIDGKDIK SLKEQVMDLG IPASELIKTA WASASTFRVT 490 500 510 520 530 540 DYRGGNNGAR IRLQPEINWE VNEPEKLKKV LASLTSLQRE FNKKQSDGKK VSLADLIVLS 550 560 570 580 590 600 GNAAIEDAAR KAGVELEIPF TPGRTDASQE QTDVASFSVL EPTADGFRNY YSKSRSHISP 610 620 630 640 650 660 VESLIDKASQ LDLTVPEMTA LLGGLRVMDI NTNNSSLGVF TDTPGVLDNK FFVNLLDMST 670 680 690 700 710 720 RWSKADKEDT YNGFDRKTGA LKWKASSVDL IFSSNPELRA VAEVYASDDA RNKFIHDFVK 730 SWNKVMNSDR FDLNNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"KatP, a novel catalase-peroxidase encoded by the large plasmid of enterohaemorrhagic Escherichia coli O157:H7." Brunder W., Schmidt H., Karch H. Microbiology 142:3305-3315(1996) [PubMed: 8969527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]	"The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7." Burland V., Shao Y., Perna N.T., Plunkett G. III, Sofia H.J., Blattner F.R. Nucleic Acids Res. 26:4196-4204(1998) [PubMed: 9722640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[3]	"Complete nucleotide sequences of 93-kb and 3.3-kb plasmids of an enterohemorrhagic Escherichia coli O157:H7 derived from Sakai outbreak." Makino K., Ishii K., Yasunaga T., Hattori M., Yokoyama K., Yatsudo H.C., Kubota Y., Yamaichi Y., Iida T., Yamamoto K., Honda T., Han C.G., Ohtsubo A., Kasamatsu M., Hayashi T., Kuhara S., Shinagawa H. DNA Res. 5:1-9(1998) [PubMed: 9628576] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X89017 Genomic DNA. Translation: CAA61429.1. AF074613 Genomic DNA. Translation: AAC70085.1. AB011549 Genomic DNA. Translation: BAA31832.1.
PIR	T00313.
RefSeq	YP_002756743.1. NC_012487.1. YP_325577.1. NC_007414.1.
3D structure databases
HSSP	HSSP built from PDB template 1ITK based on UniProtKB O59651.
ProteinModelPortal	Q7BSW8.
SMR	Q7BSW8. Positions 33-733.
ModBase	Search...
Protein family/group databases
PeroxiBase	2386. EcoH7CP02_EDL933.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000029387; EBESCP00000028092; EBESCG00000028437. EBESCT00000060752; EBESCP00000058396; EBESCG00000059799.
GeneID	3683716. 7701572.
GenomeReviews	Gene locus ECO57PM75 in contig AB011549_GR. Gene locus L7017 in contig AF074613_GR.
KEGG	ece:L7017.
PATRIC	18360409. VBIEscCol44059_5606.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000011618.
HOGENOM	HBG285610.
OMA	KNKCGKG.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG2_ECO57

Accession

Primary (citable) accession number: Q7BSW8
Secondary accession number(s): P77038

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program