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Protein

Catalase-peroxidase 2

Gene

katG2

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.UniRule annotation1 Publication

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotation
2 H2O2 = O2 + 2 H2O.UniRule annotation

Cofactori

heme bUniRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei99 – 991Transition state stabilizerUniRule annotation
Active sitei103 – 1031Proton acceptorUniRule annotation
Metal bindingi264 – 2641Iron (heme axial ligand)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Heme, Iron, Metal-binding

Protein family/group databases

PeroxiBasei2386. EcoH7CP02_EDL933.

Names & Taxonomyi

Protein namesi
Recommended name:
Catalase-peroxidase 2UniRule annotation (EC:1.11.1.21UniRule annotation)
Short name:
CP 2UniRule annotation
Alternative name(s):
Peroxidase/catalase 2UniRule annotation
Gene namesi
Name:katG2UniRule annotation
Synonyms:katP
Ordered Locus Names:L7017, ECO57PM75
Encoded oniPlasmid pO1573 Publications
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Plasmid pO157
  • UP000002519 Componenti: Plasmid pO157

Subcellular locationi

  • Periplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323UniRule annotationAdd
BLAST
Chaini24 – 736713Catalase-peroxidase 2PRO_0000354782Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki102 ↔ 223Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-249)UniRule annotation
Cross-linki223 ↔ 249Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-102)UniRule annotation

Post-translational modificationi

The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi155864.L7017.

Structurei

3D structure databases

ProteinModelPortaliQ7BSW8.
SMRiQ7BSW8. Positions 33-733.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105C1X. Bacteria.
COG0376. LUCA.
HOGENOMiHOG000218110.
KOiK03782.
OMAiMSTRWEK.
OrthoDBiEOG6RRKKM.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7BSW8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKKTLPVLI LLALSGSFST AVAADKKETQ NFYYPETLDL TPLRLHSPES
60 70 80 90 100
NPWGADFDYA TRFQQLDMEA LKKDIKDLLT TSQDWWPADY GHYGPFFIRM
110 120 130 140 150
AWHGAGTYRT YDGRGGASGG QQRFEPLNSW PDNVNLDKAR RLLWPVKKKY
160 170 180 190 200
GSSISWGDLM VLTGNVALES MGFKTLGFAG GREDDWESDL VYWGPDNKPL
210 220 230 240 250
ADNRDKNGKL QKPLAATQMG LIYVNPEGPG GKPDPLASAK DIREAFSRMA
260 270 280 290 300
MDDEETVALI AGGHTFGKAH GAASPEKCIG AGPDGAPVEE QGLGWKNKCG
310 320 330 340 350
TGNGKYTITS GLEGAWSTSP TQFTMQYLKN LYKYEWELHK SPAGAYQWKP
360 370 380 390 400
KKAANIVQDA HDPSVLHPLM MFTTDIALKV DPEYKKITTR FLNDPKAFEQ
410 420 430 440 450
AFARAWFKLT HRDMGPAARY LGNEVPAESF IWQDPLPAAD YTMIDGKDIK
460 470 480 490 500
SLKEQVMDLG IPASELIKTA WASASTFRVT DYRGGNNGAR IRLQPEINWE
510 520 530 540 550
VNEPEKLKKV LASLTSLQRE FNKKQSDGKK VSLADLIVLS GNAAIEDAAR
560 570 580 590 600
KAGVELEIPF TPGRTDASQE QTDVASFSVL EPTADGFRNY YSKSRSHISP
610 620 630 640 650
VESLIDKASQ LDLTVPEMTA LLGGLRVMDI NTNNSSLGVF TDTPGVLDNK
660 670 680 690 700
FFVNLLDMST RWSKADKEDT YNGFDRKTGA LKWKASSVDL IFSSNPELRA
710 720 730
VAEVYASDDA RNKFIHDFVK SWNKVMNSDR FDLNNK
Length:736
Mass (Da):81,794
Last modified:July 5, 2004 - v1
Checksum:iF3EA593D3FDEDB8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89017 Genomic DNA. Translation: CAA61429.1.
AF074613 Genomic DNA. Translation: AAC70085.1.
AB011549 Genomic DNA. Translation: BAA31832.1.
PIRiT00313.
RefSeqiWP_000592771.1. NZ_LKAL01000090.1.
YP_002756743.1. NC_012487.1.

Genome annotation databases

EnsemblBacteriaiAAC70085; AAC70085; Z_L7017.
BAA31832; BAA31832; BAA31832.
GeneIDi7701572.
KEGGiece:Z_L7017.
pg:7701572.
PATRICi18360409. VBIEscCol44059_5606.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89017 Genomic DNA. Translation: CAA61429.1.
AF074613 Genomic DNA. Translation: AAC70085.1.
AB011549 Genomic DNA. Translation: BAA31832.1.
PIRiT00313.
RefSeqiWP_000592771.1. NZ_LKAL01000090.1.
YP_002756743.1. NC_012487.1.

3D structure databases

ProteinModelPortaliQ7BSW8.
SMRiQ7BSW8. Positions 33-733.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.L7017.

Protein family/group databases

PeroxiBasei2386. EcoH7CP02_EDL933.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC70085; AAC70085; Z_L7017.
BAA31832; BAA31832; BAA31832.
GeneIDi7701572.
KEGGiece:Z_L7017.
pg:7701572.
PATRICi18360409. VBIEscCol44059_5606.

Phylogenomic databases

eggNOGiENOG4105C1X. Bacteria.
COG0376. LUCA.
HOGENOMiHOG000218110.
KOiK03782.
OMAiMSTRWEK.
OrthoDBiEOG6RRKKM.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "KatP, a novel catalase-peroxidase encoded by the large plasmid of enterohaemorrhagic Escherichia coli O157:H7."
    Brunder W., Schmidt H., Karch H.
    Microbiology 142:3305-3315(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
    Plasmid: pO157
  2. "The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7."
    Burland V., Shao Y., Perna N.T., Plunkett G. III, Sofia H.J., Blattner F.R.
    Nucleic Acids Res. 26:4196-4204(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
    Plasmid: pO157
  3. "Complete nucleotide sequences of 93-kb and 3.3-kb plasmids of an enterohemorrhagic Escherichia coli O157:H7 derived from Sakai outbreak."
    Makino K., Ishii K., Yasunaga T., Hattori M., Yokoyama K., Yatsudo H.C., Kubota Y., Yamaichi Y., Iida T., Yamamoto K., Honda T., Han C.G., Ohtsubo A., Kasamatsu M., Hayashi T., Kuhara S., Shinagawa H.
    DNA Res. 5:1-9(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.
    Plasmid: pO157

Entry informationi

Entry nameiKATG2_ECO57
AccessioniPrimary (citable) accession number: Q7BSW8
Secondary accession number(s): P77038
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.