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Protein

Serine protease EspP

Gene

espP

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease capable of cleaving pepsin A and human coagulation factor V, which may contribute to the mucosal hemorrhage observed in hemorrhagic colitis.2 Publications

Enzyme regulationi

Inhibition of cytotoxic activity by phenylmethylsulfonyl fluoride.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei127Charge relay systemPROSITE-ProRule annotation1
Active sitei156Charge relay systemPROSITE-ProRule annotation1
Active sitei263Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Virulence

Protein family/group databases

MEROPSiS06.002.
TCDBi1.B.12.4.3. the autotransporter-1 (at-1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease EspP (EC:3.4.21.-)
Cleaved into the following 2 chains:
Alternative name(s):
Extracellular serine protease plasmid-encoded EspP
Gene namesi
Name:espP
Ordered Locus Names:L7020, ECO57PM78
Encoded oniPlasmid pO1570 Publication
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Plasmid pO157
  • UP000002519 Componenti: Plasmid pO157

Subcellular locationi

Autotransporter protein EspP translocator :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane, Periplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 551 PublicationAdd BLAST55
ChainiPRO_000038759456 – 1300Serine protease EspPAdd BLAST1245
ChainiPRO_000004202056 – 1023Secreted autotransporter protein EspPAdd BLAST968
ChainiPRO_00000420211024 – 1300Autotransporter protein EspP translocatorBy similarityAdd BLAST277

Post-translational modificationi

Cleaved to release the mature protein from the outer membrane.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei1023 – 1024Cleavage2

Keywords - PTMi

Zymogen

Proteomic databases

PRIDEiQ7BSW5.

Expressioni

Inductioni

Expression is optimal at 37 degrees Celsius, and inhibited at 20 degrees Celsius. It is dependent on both temperature and pH.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
surAP0ABZ83EBI-6408724,EBI-6408783

Protein-protein interaction databases

IntActiQ7BSW5. 4 interactors.
STRINGi155864.L7020.

Structurei

Secondary structure

11300
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi57 – 59Combined sources3
Beta strandi61 – 63Combined sources3
Helixi65 – 73Combined sources9
Beta strandi86 – 88Combined sources3
Beta strandi94 – 96Combined sources3
Beta strandi121 – 125Combined sources5
Helixi126 – 128Combined sources3
Helixi133 – 136Combined sources4
Beta strandi137 – 140Combined sources4
Beta strandi143 – 153Combined sources11
Turni154 – 156Combined sources3
Beta strandi157 – 164Combined sources8
Helixi182 – 189Combined sources8
Beta strandi191 – 198Combined sources8
Beta strandi200 – 205Combined sources6
Beta strandi208 – 213Combined sources6
Beta strandi216 – 220Combined sources5
Helixi227 – 229Combined sources3
Beta strandi231 – 248Combined sources18
Beta strandi251 – 254Combined sources4
Beta strandi266 – 271Combined sources6
Turni272 – 275Combined sources4
Beta strandi276 – 288Combined sources13
Helixi290 – 292Combined sources3
Beta strandi294 – 300Combined sources7
Helixi303 – 312Combined sources10
Beta strandi314 – 318Combined sources5
Beta strandi324 – 328Combined sources5
Beta strandi331 – 337Combined sources7
Beta strandi344 – 351Combined sources8
Beta strandi353 – 359Combined sources7
Beta strandi368 – 370Combined sources3
Beta strandi375 – 379Combined sources5
Beta strandi384 – 388Combined sources5
Beta strandi390 – 392Combined sources3
Beta strandi397 – 400Combined sources4
Beta strandi402 – 404Combined sources3
Beta strandi410 – 420Combined sources11
Beta strandi428 – 437Combined sources10
Beta strandi446 – 457Combined sources12
Beta strandi466 – 468Combined sources3
Beta strandi471 – 473Combined sources3
Beta strandi478 – 481Combined sources4
Beta strandi487 – 490Combined sources4
Beta strandi499 – 502Combined sources4
Beta strandi505 – 507Combined sources3
Beta strandi509 – 513Combined sources5
Beta strandi519 – 521Combined sources3
Beta strandi523 – 534Combined sources12
Beta strandi544 – 546Combined sources3
Beta strandi548 – 551Combined sources4
Beta strandi556 – 560Combined sources5
Beta strandi562 – 565Combined sources4
Helixi596 – 603Combined sources8
Helixi605 – 611Combined sources7
Helixi614 – 617Combined sources4
Beta strandi632 – 643Combined sources12
Beta strandi645 – 648Combined sources4
Beta strandi652 – 662Combined sources11
Beta strandi664 – 668Combined sources5
Beta strandi670 – 675Combined sources6
Turni676 – 679Combined sources4
Turni684 – 687Combined sources4
Beta strandi693 – 697Combined sources5
Beta strandi702 – 706Combined sources5
Beta strandi708 – 712Combined sources5
Beta strandi714 – 717Combined sources4
Beta strandi721 – 725Combined sources5
Beta strandi729 – 733Combined sources5
Beta strandi735 – 738Combined sources4
Beta strandi743 – 746Combined sources4
Beta strandi750 – 754Combined sources5
Beta strandi756 – 765Combined sources10
Beta strandi777 – 787Combined sources11
Beta strandi793 – 810Combined sources18
Beta strandi813 – 818Combined sources6
Helixi832 – 839Combined sources8
Beta strandi842 – 848Combined sources7
Beta strandi850 – 866Combined sources17
Beta strandi870 – 879Combined sources10
Beta strandi881 – 884Combined sources4
Beta strandi895 – 905Combined sources11
Beta strandi907 – 915Combined sources9
Beta strandi919 – 927Combined sources9
Beta strandi931 – 939Combined sources9
Beta strandi943 – 945Combined sources3
Beta strandi949 – 955Combined sources7
Helixi960 – 962Combined sources3
Beta strandi963 – 965Combined sources3
Beta strandi975 – 983Combined sources9
Beta strandi985 – 997Combined sources13
Helixi999 – 1020Combined sources22
Helixi1025 – 1029Combined sources5
Beta strandi1038 – 1052Combined sources15
Helixi1053 – 1055Combined sources3
Beta strandi1056 – 1073Combined sources18
Beta strandi1076 – 1093Combined sources18
Beta strandi1096 – 1113Combined sources18
Beta strandi1116 – 1134Combined sources19
Helixi1136 – 1138Combined sources3
Beta strandi1140 – 1157Combined sources18
Beta strandi1159 – 1178Combined sources20
Beta strandi1182 – 1187Combined sources6
Beta strandi1190 – 1196Combined sources7
Beta strandi1202 – 1216Combined sources15
Beta strandi1219 – 1233Combined sources15
Beta strandi1238 – 1242Combined sources5
Beta strandi1247 – 1251Combined sources5
Beta strandi1256 – 1268Combined sources13
Turni1269 – 1271Combined sources3
Beta strandi1272 – 1300Combined sources29

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QOMX-ray2.66A/B1024-1300[»]
3SLJX-ray2.48A999-1300[»]
3SLOX-ray2.52A999-1300[»]
3SLTX-ray2.46A999-1300[»]
3SZEX-ray2.50A56-1023[»]
ProteinModelPortaliQ7BSW5.
SMRiQ7BSW5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7BSW5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini57 – 311Peptidase S6PROSITE-ProRule annotationAdd BLAST255
Domaini1034 – 1300AutotransporterPROSITE-ProRule annotationAdd BLAST267

Domaini

The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage (PubMed:15615856).1 Publication

Sequence similaritiesi

Contains 1 autotransporter (TC 1.B.12) domain. [View classification]PROSITE-ProRule annotation
Contains 1 peptidase S6 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105I1J. Bacteria.
COG3468. LUCA.
HOGENOMiHOG000117212.
KOiK12684.
OMAiLVMQGHA.

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR024973. ESPR.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR000710. Peptidase_S6.
IPR030396. Peptidase_S6_dom.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF13018. ESPR. 1 hit.
PF02395. Peptidase_S6. 1 hit.
[Graphical view]
PRINTSiPR00921. IGASERPTASE.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 2 hits.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS51691. PEPTIDASE_S6. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7BSW5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKIYSLKYS HITGGLIAVS ELSGRVSSRA TGKKKHKRIL ALCFLGLLQS
60 70 80 90 100
SYSFASQMDI SNFYIRDYMD FAQNKGIFQA GATNIEIVKK DGSTLKLPEV
110 120 130 140 150
PFPDFSPVAN KGSTTSIGGA YSITATHNTK NHHSVATQNW GNSTYKQTDW
160 170 180 190 200
NTSHPDFAVS RLDKFVVETR GATEGADISL SKQQALERYG VNYKGEKKLI
210 220 230 240 250
AFRAGSGVVS VKKNGRITPF NEVSYKPEML NGSFVHIDDW SGWLILTNNQ
260 270 280 290 300
FDEFNNIASQ GDSGSALFVY DNQKKKWVVA GTVWGIYNYA NGKNHAAYSK
310 320 330 340 350
WNQTTIDNLK NKYSYNVDMS GAQVATIENG KLTGTGSDTT DIKNKDLIFT
360 370 380 390 400
GGGDILLKSS FDNGAGGLVF NDKKTYRVNG DDFTFKGAGV DTRNGSTVEW
410 420 430 440 450
NIRYDNKDNL HKIGDGTLDV RKTQNTNLKT GEGLVILGAE KTFNNIYITS
460 470 480 490 500
GDGTVRLNAE NALSGGEYNG IFFAKNGGTL DLNGYNQSFN KIAATDSGAV
510 520 530 540 550
ITNTSTKKSI LSLNNTADYI YHGNINGNLD VLQHHETKKE NRRLILDGGV
560 570 580 590 600
DTTNDISLRN TQLSMQGHAT EHAIYRDGAF SCSLPAPMRF LCGSDYVAGM
610 620 630 640 650
QNTEADAVKQ NGNAYKTNNA VSDLSQPDWE TGTFRFGTLH LENSDFSVGR
660 670 680 690 700
NANVIGDIQA SKSNITIGDT TAYIDLHAGK NITGDGFGFR QNIVRGNSQG
710 720 730 740 750
ETLFTGGITA EDSTIVIKDK AKALFSNYVY LLNTKATIEN GADVTTQSGM
760 770 780 790 800
FSTSDISISG NLSMTGNPDK DNKFEPSIYL NDASYLLTDD SARLVAKNKA
810 820 830 840 850
SVVGDIHSTK SASIMFGHDE SDLSQLSDRT SKGLALGLLG GFDVSYRGSV
860 870 880 890 900
NAPSASATMN NTWWQLTGDS ALKTLKSTNS MVYFTDSANN KKFHTLTVDE
910 920 930 940 950
LATSNSAYAM RTNLSESDKL EVKKHLSGEN NILLVDFLQK PTPEKQLNIE
960 970 980 990 1000
LVSAPKDTNE NVFKASKQTI GFSDVTPVIT TRETDDKITW SLTGYNTVAN
1010 1020 1030 1040 1050
KEATRNAAAL FSVDYKAFLN EVNNLNKRMG DLRDINGEAG AWARIMSGTG
1060 1070 1080 1090 1100
SASGGFSDNY THVQVGVDKK HELDGLDLFT GFTVTHTDSS ASADVFSGKT
1110 1120 1130 1140 1150
KSVGAGLYAS AMFDSGAYID LIGKYVHHDN EYTATFAGLG TRDYSTHSWY
1160 1170 1180 1190 1200
AGAEAGYRYH VTEDAWIEPQ AELVYGSVSG KQFAWKDQGM HLSMKDKDYN
1210 1220 1230 1240 1250
PLIGRTGVDV GKSFSGKDWK VTARAGLGYQ FDLLANGETV LRDASGEKRI
1260 1270 1280 1290 1300
KGEKDSRMLM SVGLNAEIRD NVRFGLEFEK SAFGKYNVDN AVNANFRYSF
Length:1,300
Mass (Da):141,758
Last modified:July 5, 2004 - v1
Checksum:iE34D3F037DEC672F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97542 Genomic DNA. Translation: CAA66144.1.
AF074613 Genomic DNA. Translation: AAC70088.1.
AB011549 Genomic DNA. Translation: BAA31836.1.
AJ010390 Genomic DNA. Translation: CAB42538.1.
PIRiT00317.
RefSeqiNP_052685.1. NC_002128.1.
WP_001034100.1. NZ_LKAL01000035.1.
YP_002756610.1. NC_012487.1.

Genome annotation databases

EnsemblBacteriaiAAC70088; AAC70088; Z_L7020.
BAA31836; BAA31836; BAA31836.
GeneIDi1789732.
7701441.
KEGGiece:Z_L7020.
ecs:pO157p78.
PATRICi18360417. VBIEscCol44059_5610.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97542 Genomic DNA. Translation: CAA66144.1.
AF074613 Genomic DNA. Translation: AAC70088.1.
AB011549 Genomic DNA. Translation: BAA31836.1.
AJ010390 Genomic DNA. Translation: CAB42538.1.
PIRiT00317.
RefSeqiNP_052685.1. NC_002128.1.
WP_001034100.1. NZ_LKAL01000035.1.
YP_002756610.1. NC_012487.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QOMX-ray2.66A/B1024-1300[»]
3SLJX-ray2.48A999-1300[»]
3SLOX-ray2.52A999-1300[»]
3SLTX-ray2.46A999-1300[»]
3SZEX-ray2.50A56-1023[»]
ProteinModelPortaliQ7BSW5.
SMRiQ7BSW5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7BSW5. 4 interactors.
STRINGi155864.L7020.

Protein family/group databases

MEROPSiS06.002.
TCDBi1.B.12.4.3. the autotransporter-1 (at-1) family.

Proteomic databases

PRIDEiQ7BSW5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC70088; AAC70088; Z_L7020.
BAA31836; BAA31836; BAA31836.
GeneIDi1789732.
7701441.
KEGGiece:Z_L7020.
ecs:pO157p78.
PATRICi18360417. VBIEscCol44059_5610.

Phylogenomic databases

eggNOGiENOG4105I1J. Bacteria.
COG3468. LUCA.
HOGENOMiHOG000117212.
KOiK12684.
OMAiLVMQGHA.

Miscellaneous databases

EvolutionaryTraceiQ7BSW5.

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR024973. ESPR.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR000710. Peptidase_S6.
IPR030396. Peptidase_S6_dom.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF13018. ESPR. 1 hit.
PF02395. Peptidase_S6. 1 hit.
[Graphical view]
PRINTSiPR00921. IGASERPTASE.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 2 hits.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS51691. PEPTIDASE_S6. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiESPP_ECO57
AccessioniPrimary (citable) accession number: Q7BSW5
Secondary accession number(s): O32555, Q9S6R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Mutagenesis experiments show the presence of a linker region, which is required for the translocation of the protease domain across the outer membrane. This linker region is located immediately upstream of the C-terminal helper domain.
The signal peptide may also act as a transient membrane anchor that prevents the protease domain from misfolding in the periplasm.

Caution

Strain 3010/96 exhibits a partial deletion of the gene.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.