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Protein

Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD

Gene

arnD

Organism
Yersinia pseudotuberculosis serotype I (strain IP32953)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the deformylation of 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol to 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides.UniRule annotation

Catalytic activityi

4-deoxy-4-formamido-beta-L-arabinose di-trans,poly-cis-undecaprenyl phosphate + H2O = 4-amino-4-deoxy-alpha-L-arabinose di-trans,poly-cis-undecaprenyl phosphate + formate.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides Source: UniProtKB-HAMAP

GO - Biological processi

  1. 4-amino-4-deoxy-alpha-L-arabinopyranosyl undecaprenyl phosphate biosynthetic process Source: UniProtKB-UniPathway
  2. lipid A biosynthetic process Source: UniProtKB-HAMAP
  3. lipopolysaccharide biosynthetic process Source: UniProtKB-UniPathway
  4. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance, Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciYPSE273123:GI1M-2413-MONOMER.
UniPathwayiUPA00030.
UPA00036; UER00496.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnDUniRule annotation (EC:3.5.1.n3UniRule annotation)
Gene namesi
Name:arnDUniRule annotation
Ordered Locus Names:YPTB2327
OrganismiYersinia pseudotuberculosis serotype I (strain IP32953)
Taxonomic identifieri273123 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000001011 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 301301Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnDPRO_0000383556Add
BLAST

Expressioni

Inductioni

Activated by low magnesium concentrations, via the two-component regulatory system PhoP/PhoQ.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi273123.YPTB2327.

Structurei

3D structure databases

ProteinModelPortaliQ7BF86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 261260NodB homologyUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polysaccharide deacetylase family. ArnD deformylase subfamily.UniRule annotation
Contains 1 NodB homology domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0726.
HOGENOMiHOG000261199.
KOiK13014.
OMAiLHAWDHF.
OrthoDBiEOG6423D0.

Family and domain databases

Gene3Di3.20.20.370. 2 hits.
HAMAPiMF_01870. ArnD.
InterProiIPR023557. ArnD.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR002509. Polysac_deacetylase.
[Graphical view]
PfamiPF01522. Polysacc_deac_1. 1 hit.
[Graphical view]
SUPFAMiSSF88713. SSF88713. 2 hits.
PROSITEiPS51677. NODB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7BF86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQVGLRIDV DTYRGTQYGV PSLLTVLEKH DIRASFFFSV GPDNMGRHLW
60 70 80 90 100
RLFRPRFLWK MLRSNAASLY GWDILLAGTA WPGKKIAKDF GPLMKAAAMA
110 120 130 140 150
GHEVGLHAWD HQGWQANVAS WSQQQLTEQV QRGVDTLQQS IGQPISCSAA
160 170 180 190 200
AGWRADERVL AVKQQFDFSY NSDCRGTHPF RPLLPNGSLG SVQIPVTLPT
210 220 230 240 250
YDEVVGGEVQ AENFNDFIID AILRDSGVSV YTIHAEVEGM SQAAMFEQLL
260 270 280 290 300
MRAKQQDIEF CPLSKLLPSD LQLLPVGKVI RAAFPGREGW LGCQSDIKDA

E
Length:301
Mass (Da):33,504
Last modified:July 5, 2004 - v1
Checksum:iABA3A1132D09A864
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF336802 Genomic DNA. Translation: AAK69643.1.
BX936398 Genomic DNA. Translation: CAH21565.1.
RefSeqiYP_070842.1. NC_006155.1.

Genome annotation databases

EnsemblBacteriaiCAH21565; CAH21565; YPTB2327.
KEGGiyps:YPTB2327.
PATRICi18643570. VBIYerPse22266_2843.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF336802 Genomic DNA. Translation: AAK69643.1.
BX936398 Genomic DNA. Translation: CAH21565.1.
RefSeqiYP_070842.1. NC_006155.1.

3D structure databases

ProteinModelPortaliQ7BF86.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273123.YPTB2327.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAH21565; CAH21565; YPTB2327.
KEGGiyps:YPTB2327.
PATRICi18643570. VBIYerPse22266_2843.

Phylogenomic databases

eggNOGiCOG0726.
HOGENOMiHOG000261199.
KOiK13014.
OMAiLHAWDHF.
OrthoDBiEOG6423D0.

Enzyme and pathway databases

UniPathwayiUPA00030.
UPA00036; UER00496.
BioCyciYPSE273123:GI1M-2413-MONOMER.

Family and domain databases

Gene3Di3.20.20.370. 2 hits.
HAMAPiMF_01870. ArnD.
InterProiIPR023557. ArnD.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR002509. Polysac_deacetylase.
[Graphical view]
PfamiPF01522. Polysacc_deac_1. 1 hit.
[Graphical view]
SUPFAMiSSF88713. SSF88713. 2 hits.
PROSITEiPS51677. NODB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The pmrF polymyxin-resistance operon of Yersinia pseudotuberculosis is upregulated by the PhoP-PhoQ two-component system but not by PmrA-PmrB, and is not required for virulence."
    Marceau M.B., Sebbane F., Ewann F., Collyn F., Lindner B., Campos M.A., Bengoechea J.-A., Simonet M.
    Microbiology 150:3947-3957(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
    Strain: 32777 / IP2777 / Serotype O1:b.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IP32953.

Entry informationi

Entry nameiARND_YERPS
AccessioniPrimary (citable) accession number: Q7BF86
Secondary accession number(s): Q66A05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 5, 2004
Last modified: April 1, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.