Q7BBW0 (DIVK_BRUAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polar-differentiation response regulator DivK

Gene names

Name:	divK
Ordered Locus Names:	BruAb2_0613

Organism

Brucella abortus biovar 1 (strain 9-941) [Complete proteome] [HAMAP]

Taxonomic identifier

262698 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella abortus › Brucella abortus bv. 1

Protein attributes

Sequence length	123 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential protein that is involved in the control of cell division, probably through the regulation of ctrA. Its phosphorylation status is regulated by PdhS. Ref.4
Subunit structure	Interacts with DivL, PleC, DivJ and PdhS. Ref.4
Subcellular location	Cytoplasm. Note: Localized at one pole of the cell. Colocalizes with PdhS. Ref.4
Disruption phenotype	Lethal. Ref.4
Sequence similarities	Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Cellular component	Cytoplasm
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 123	123	Polar-differentiation response regulator DivK		PRO_0000363206
Regions
	Domain	4 – 120	117	Response regulatory
Amino acid modifications
	Modified residue	53	1	4-aspartylphosphate
Experimental info
	Mutagenesis	53	1	D → A: Abolishes phosphorylation and localization to the cell pole. Ref.4
	Sequence conflict	92	1	E → R in AAF14690. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q7BBW0 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 0D74511EB774FA24	FASTA	123	13,973
	10 20 30 40 50 60 MTKSVMIVED NELNMKLFRD LIEASGYETI RTRSGLEALD LAREHHPDLI LMDIQLPEVS 70 80 90 100 110 120 GLEVTKWLKD DEELRHIPVI AVTAFAMKGD EERIRQGGCE AYISKPISVP RFIETIKSYL GDA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A phosphorelay in Brucella species." Devos D.P., Rousseau C., Depiereux E., Letesson J.-J. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Plasticity of a transcriptional regulation network among alpha-proteobacteria is supported by the identification of CtrA targets in Brucella abortus." Bellefontaine A.F., Pierreux C.E., Mertens P., Vandenhaute J., Letesson J.J., De Bolle X. Mol. Microbiol. 43:945-960(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 544 / Biovar 1.
[3]	"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941.
[4]	"The asymmetric distribution of the essential histidine kinase PdhS indicates a differentiation event in Brucella abortus." Hallez R., Mignolet J., Van Mullem V., Wery M., Vandenhaute J., Letesson J.-J., Jacobs-Wagner C., De Bolle X. EMBO J. 26:1444-1455(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN CONTROL OF CELL SHAPE, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, PHOSPHORYLATION, INTERACTION WITH DIVL/PLEC/DIVJ/PDHS, MUTAGENESIS OF ASP-53. Strain: 544 / Biovar 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF051940 mRNA. Translation: AAF14690.1. AF411569 Genomic DNA. Translation: AAL86375.1. AE017224 Genomic DNA. Translation: AAX76024.1.
RefSeq	YP_223385.1. NC_006933.1.
3D structure databases
HSSP	HSSP built from PDB template 1OXK based on UniProtKB P39928.
ProteinModelPortal	Q7BBW0.
SMR	Q7BBW0. Positions 2-120.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4507690.
STRING	262698.BruAb2_0613.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAX76024; AAX76024; BruAb2_0613.
GeneID	3342309.
KEGG	bmb:BruAb2_0613.
PATRIC	17827503. VBIBruAbo15061_2926.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0784.
HOGENOM	HOG000034820.
KO	K11443.
OMA	WIKDDPE.
ProtClustDB	CLSK863111.
Enzyme and pathway databases
BioCyc	BABO262698:GJC2-2827-MONOMER.
Family and domain databases
InterPro	IPR011006. CheY-like_superfamily. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Pfam	PF00072. Response_reg. 1 hit. [Graphical view]
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52172. CheY_like. 1 hit.
PROSITE	PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DIVK_BRUAB

Accession

Primary (citable) accession number: Q7BBW0
Secondary accession number(s): Q578B0, Q9RHA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program