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Q7AKQ6

- PSA_STRCO

UniProt

Q7AKQ6 - PSA_STRCO

Protein

Proteasome subunit alpha

Gene

prcA

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The S.coelicolor proteasome is able to cleave oligopeptides after hydrophobic residues, but not after basic or acidic residues, thus displaying chymotrypsin-like activity but not trypsin-like activity.1 PublicationUniRule annotation

    Catalytic activityi

    Cleavage of peptide bonds with very broad specificity.1 PublicationUniRule annotation

    Enzyme regulationi

    The formation of the proteasomal ATPase ARC-20S proteasome complex, likely via the docking of the C-termini of ARC into the intersubunit pockets in the alpha-rings, may trigger opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity By similarity. Peptidolytic activity is completely inhibited by lactacystin, and to a lesser extent, by N-acetyl-Leu-Leu-norleucinal (Ac-LLnL) and benzoyloxycarbonyl-Leu-Leu-Leu-vinylsulfone (Z-LLL-VS) in vitro.1 PublicationUniRule annotation

    Temperature dependencei

    Optimum temperature is 55 degrees Celsius.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. threonine-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. proteolysis involved in cellular protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Threonine protease

    Enzyme and pathway databases

    UniPathwayiUPA00997.

    Protein family/group databases

    MEROPSiT01.011.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteasome subunit alphaUniRule annotation (EC:3.4.25.1UniRule annotation)
    Alternative name(s):
    20S proteasome alpha subunitUniRule annotation
    Proteasome core protein PrcAUniRule annotation
    Gene namesi
    Name:prcAUniRule annotation
    Ordered Locus Names:SCO1643
    ORF Names:SCI41.26
    OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
    Taxonomic identifieri100226 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
    ProteomesiUP000001973: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. proteasome core complex, alpha-subunit complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 254254Proteasome subunit alphaPRO_0000383484Add
    BLAST

    Post-translational modificationi

    The N-terminus is blocked.

    Proteomic databases

    PRIDEiQ7AKQ6.

    PTM databases

    PhosSiteiP12011285.

    Interactioni

    Subunit structurei

    The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has probably a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is likely capped by the proteasome-associated ATPase, ARC.1 Publication

    Protein-protein interaction databases

    STRINGi100226.SCO1643.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7AKQ6.
    SMRiQ7AKQ6. Positions 8-232.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase T1A family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0638.
    HOGENOMiHOG000245319.
    KOiK03432.
    OMAiGRYNEFE.
    OrthoDBiEOG6NKQZG.
    PhylomeDBiQ7AKQ6.

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    HAMAPiMF_00289_B. Proteasome_A_B.
    InterProiIPR029055. Ntn_hydrolases_N.
    IPR022296. Proteasome_asu_bac.
    IPR023332. Proteasome_suA-type.
    IPR001353. Proteasome_sua/b.
    [Graphical view]
    PfamiPF00227. Proteasome. 1 hit.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR03691. 20S_bact_alpha. 1 hit.
    PROSITEiPS51475. PROTEASOME_ALPHA_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7AKQ6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTPFYVSPQ QAMADRAEYA RKGIARGRSL VVLQYADGIV FVGENPSRAL    50
    HKFSEIYDRI GFAAAGKYNE YENLRIGGVR YADLRGYTYD RDDVTARGLA 100
    NVYAQTLGTI FSSQAEKPYE VELVVAEVGD SPENDQIYRL PHDGSIVDEH 150
    GSVAVGGNAE QISGYLDQRH RDGMTLAEAL KLAVQALSRD TNGTEREIPA 200
    ERLEVAVLDR TRPQQRKFKR IVGGQLSRLL ESGAASADGE AETEAETDSG 250
    SDEE 254
    Length:254
    Mass (Da):27,883
    Last modified:July 5, 2004 - v1
    Checksum:i97460DA9883C33D8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF086832 Genomic DNA. Translation: AAC64278.1.
    AL939109 Genomic DNA. Translation: CAB59496.1.
    RefSeqiNP_625918.1. NC_003888.3.
    WP_011027897.1. NC_003888.3.

    Genome annotation databases

    EnsemblBacteriaiCAB59496; CAB59496; CAB59496.
    GeneIDi1097074.
    KEGGisco:SCO1643.
    PATRICi23732888. VBIStrCoe124346_1658.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF086832 Genomic DNA. Translation: AAC64278.1 .
    AL939109 Genomic DNA. Translation: CAB59496.1 .
    RefSeqi NP_625918.1. NC_003888.3.
    WP_011027897.1. NC_003888.3.

    3D structure databases

    ProteinModelPortali Q7AKQ6.
    SMRi Q7AKQ6. Positions 8-232.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 100226.SCO1643.

    Protein family/group databases

    MEROPSi T01.011.

    PTM databases

    PhosSitei P12011285.

    Proteomic databases

    PRIDEi Q7AKQ6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB59496 ; CAB59496 ; CAB59496 .
    GeneIDi 1097074.
    KEGGi sco:SCO1643.
    PATRICi 23732888. VBIStrCoe124346_1658.

    Phylogenomic databases

    eggNOGi COG0638.
    HOGENOMi HOG000245319.
    KOi K03432.
    OMAi GRYNEFE.
    OrthoDBi EOG6NKQZG.
    PhylomeDBi Q7AKQ6.

    Enzyme and pathway databases

    UniPathwayi UPA00997 .

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    HAMAPi MF_00289_B. Proteasome_A_B.
    InterProi IPR029055. Ntn_hydrolases_N.
    IPR022296. Proteasome_asu_bac.
    IPR023332. Proteasome_suA-type.
    IPR001353. Proteasome_sua/b.
    [Graphical view ]
    Pfami PF00227. Proteasome. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR03691. 20S_bact_alpha. 1 hit.
    PROSITEi PS51475. PROTEASOME_ALPHA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The 20S proteasome of Streptomyces coelicolor."
      Nagy I., Tamura T., Vanderleyden J., Baumeister W., De Mot R.
      J. Bacteriol. 180:5448-5453(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, SUBUNIT, ENZYME REGULATION, TEMPERATURE DEPENDENCE, BLOCKAGE OF N-TERMINUS.
      Strain: ATCC BAA-471 / A3(2) / M145.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-471 / A3(2) / M145.

    Entry informationi

    Entry nameiPSA_STRCO
    AccessioniPrimary (citable) accession number: Q7AKQ6
    Secondary accession number(s): O87599
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3