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Q7AKQ6

- PSA_STRCO

UniProt

Q7AKQ6 - PSA_STRCO

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Protein

Proteasome subunit alpha

Gene
prcA, SCO1643, SCI41.26
Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The S.coelicolor proteasome is able to cleave oligopeptides after hydrophobic residues, but not after basic or acidic residues, thus displaying chymotrypsin-like activity but not trypsin-like activity.1 Publication

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.1 Publication

Enzyme regulationi

The formation of the proteasomal ATPase ARC-20S proteasome complex, likely via the docking of the C-termini of ARC into the intersubunit pockets in the alpha-rings, may trigger opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity By similarity. Peptidolytic activity is completely inhibited by lactacystin, and to a lesser extent, by N-acetyl-Leu-Leu-norleucinal (Ac-LLnL) and benzoyloxycarbonyl-Leu-Leu-Leu-vinylsulfone (Z-LLL-VS) in vitro.1 Publication

Temperature dependencei

Optimum temperature is 55 degrees Celsius.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. proteolysis involved in cellular protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

UniPathwayiUPA00997.

Protein family/group databases

MEROPSiT01.011.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha (EC:3.4.25.1)
Alternative name(s):
20S proteasome alpha subunit
Proteasome core protein PrcA
Gene namesi
Name:prcA
Ordered Locus Names:SCO1643
ORF Names:SCI41.26
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. proteasome core complex, alpha-subunit complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 254254Proteasome subunit alphaUniRule annotationPRO_0000383484Add
BLAST

Post-translational modificationi

The N-terminus is blocked.UniRule annotation

Proteomic databases

PRIDEiQ7AKQ6.

PTM databases

PhosSiteiP12011285.

Interactioni

Subunit structurei

The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has probably a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is likely capped by the proteasome-associated ATPase, ARC.1 Publication

Protein-protein interaction databases

STRINGi100226.SCO1643.

Structurei

3D structure databases

ProteinModelPortaliQ7AKQ6.
SMRiQ7AKQ6. Positions 8-232.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000245319.
KOiK03432.
OMAiGRYNEFE.
OrthoDBiEOG6NKQZG.
PhylomeDBiQ7AKQ6.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00289_B. Proteasome_A_B.
InterProiIPR029055. Ntn_hydrolases_N.
IPR022296. Proteasome_asu_bac.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR03691. 20S_bact_alpha. 1 hit.
PROSITEiPS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7AKQ6-1 [UniParc]FASTAAdd to Basket

« Hide

MSTPFYVSPQ QAMADRAEYA RKGIARGRSL VVLQYADGIV FVGENPSRAL    50
HKFSEIYDRI GFAAAGKYNE YENLRIGGVR YADLRGYTYD RDDVTARGLA 100
NVYAQTLGTI FSSQAEKPYE VELVVAEVGD SPENDQIYRL PHDGSIVDEH 150
GSVAVGGNAE QISGYLDQRH RDGMTLAEAL KLAVQALSRD TNGTEREIPA 200
ERLEVAVLDR TRPQQRKFKR IVGGQLSRLL ESGAASADGE AETEAETDSG 250
SDEE 254
Length:254
Mass (Da):27,883
Last modified:July 5, 2004 - v1
Checksum:i97460DA9883C33D8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF086832 Genomic DNA. Translation: AAC64278.1.
AL939109 Genomic DNA. Translation: CAB59496.1.
RefSeqiNP_625918.1. NC_003888.3.
WP_011027897.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAB59496; CAB59496; CAB59496.
GeneIDi1097074.
KEGGisco:SCO1643.
PATRICi23732888. VBIStrCoe124346_1658.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF086832 Genomic DNA. Translation: AAC64278.1 .
AL939109 Genomic DNA. Translation: CAB59496.1 .
RefSeqi NP_625918.1. NC_003888.3.
WP_011027897.1. NC_003888.3.

3D structure databases

ProteinModelPortali Q7AKQ6.
SMRi Q7AKQ6. Positions 8-232.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 100226.SCO1643.

Protein family/group databases

MEROPSi T01.011.

PTM databases

PhosSitei P12011285.

Proteomic databases

PRIDEi Q7AKQ6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB59496 ; CAB59496 ; CAB59496 .
GeneIDi 1097074.
KEGGi sco:SCO1643.
PATRICi 23732888. VBIStrCoe124346_1658.

Phylogenomic databases

eggNOGi COG0638.
HOGENOMi HOG000245319.
KOi K03432.
OMAi GRYNEFE.
OrthoDBi EOG6NKQZG.
PhylomeDBi Q7AKQ6.

Enzyme and pathway databases

UniPathwayi UPA00997 .

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
HAMAPi MF_00289_B. Proteasome_A_B.
InterProi IPR029055. Ntn_hydrolases_N.
IPR022296. Proteasome_asu_bac.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view ]
Pfami PF00227. Proteasome. 1 hit.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR03691. 20S_bact_alpha. 1 hit.
PROSITEi PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The 20S proteasome of Streptomyces coelicolor."
    Nagy I., Tamura T., Vanderleyden J., Baumeister W., De Mot R.
    J. Bacteriol. 180:5448-5453(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, SUBUNIT, ENZYME REGULATION, TEMPERATURE DEPENDENCE, BLOCKAGE OF N-TERMINUS.
    Strain: ATCC BAA-471 / A3(2) / M145.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiPSA_STRCO
AccessioniPrimary (citable) accession number: Q7AKQ6
Secondary accession number(s): O87599
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 5, 2004
Last modified: September 3, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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