Q7ADE7 (ASTB_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-succinylarginine dihydrolase
EC=3.5.3.23

Gene names

Name:	astB
Ordered Locus Names:	Z2777, ECs2451

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the hydrolysis of N(2)-succinylarginine into N(2)-succinylornithine, ammonia and CO2 By similarity. HAMAP MF_01172
Catalytic activity	N(2)-succinyl-L-arginine + 2 H2O = N(2)-succinyl-L-ornithine + 2 NH3 + CO2. HAMAP MF_01172
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 2/5. HAMAP MF_01172
Subunit structure	Homodimer By similarity. HAMAP MF_01172
Sequence similarities	Belongs to the succinylarginine dihydrolase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	N-succinylarginine dihydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	N-succinylarginine dihydrolase HAMAP MF_01172		PRO_0000262350
Regions
	Region	19 – 28	10	Substrate binding By similarity
	Region	137 – 138	2	Substrate binding By similarity
Sites
	Active site	174	1	By similarity
	Active site	248	1	By similarity
	Active site	365	1	Nucleophile By similarity
	Binding site	110	1	Substrate By similarity
	Binding site	212	1	Substrate By similarity
	Binding site	250	1	Substrate By similarity
	Binding site	359	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7ADE7 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 6058E704A1A26CC2	FASTA	447	49,325
	10 20 30 40 50 60 MNAWEVNFDG LVGLTHHYAG LSFGNKASTR HRFQVSNPRL AAKQGLLKMK TLADAGFPQA 70 80 90 100 110 120 VIPPHERPFI PVLRQLGFSG SDEQVLEKVV RQAPHWLSSV SSASPMWVAN AATIAPSADT 130 140 150 160 170 180 LDGKVHLTVA NLNNKFHRSL EAPVTESLLK AIFNDEEKFS VHSALPQVAL LGDEGAANHN 190 200 210 220 230 240 RLGGHYGEPG MQLFVYGREE GNDTRPSRYP ARQTREASEA VARLNQVNPQ QVIFAQQNPD 250 260 270 280 290 300 VIDQGVFHND VIAVSNRQVL FCHQQAFARQ SQLLANLRAR VNGFMAIEVP ATQVSVSDAV 310 320 330 340 350 360 STYLFNSQLL SRDDGSMMLV LPQECREHAG VWGYLNELLA ADNPISELKV FDLRESMANG 370 380 390 400 410 420 GGPACLRLRV VLTQEERRAV NPAVMMNDTL FNALNDWVDR YYRDRLTAAD LADPQLLREG 430 440 REALDVLSQL LNLGSVYPFQ REGGGNG

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG56731.1. BA000007 Genomic DNA. Translation: BAB35874.1.
PIR	C90935. G85783.
RefSeq	NP_288178.1. NC_002655.2. NP_310478.1. NC_002695.1.
3D structure databases
HSSP	HSSP built from PDB template 1YNF based on UniProtKB P76216.
ProteinModelPortal	Q7ADE7.
SMR	Q7ADE7. Positions 2-441.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000024790; EBESCP00000023683; EBESCG00000023844. EBESCT00000056003; EBESCP00000053831; EBESCG00000055051.
GeneID	914117. 961716.
GenomeReviews	Gene locus Z2777 in contig AE005174_GR. Gene locus ECs2451 in contig BA000007_GR.
KEGG	ece:Z2777. ecs:ECs2451.
PATRIC	18354246. VBIEscCol44059_2334.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000010790.
HOGENOM	HBG289862.
OMA	HFAHHPA.
ProtClustDB	PRK13281.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS2451-MONOMER.
Family and domain databases
HAMAP	MF_01172. AstB. [Tree]
InterPro	IPR007079. SuccinylArg_d-Hdrlase_AstB. [Graphical view]
Gene3D	G3DSA:3.75.10.20. SuccinylArg_di_hydro. 1 hit.
KO	K01484.
Pfam	PF04996. AstB. 1 hit. [Graphical view]
TIGRFAMs	TIGR03241. Arg_catab_astB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTB_ECO57

Accession

Primary (citable) accession number: Q7ADE7
Secondary accession number(s): Q8XDY9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program