Reviewed,
UniProtKB/Swiss-Prot Q7A978 (KATG1_ECO57)
Last modified
February 9, 2010.
Version 44.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Catalase-peroxidase 1 Short name=CP 1 EC=1.11.1.6 EC=1.11.1.7 Alternative name(s): Peroxidase/catalase 1 | ||||
| Gene names |
| ||||
| Organism | Escherichia coli O157:H7 [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 83334 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 726 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961 |
| Catalytic activity | 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 |
| Cofactor | Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity. HAMAP MF_01961 |
| Subunit structure | Homodimer or homotetramer By similarity. HAMAP MF_01961 |
| Post-translational modification | The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961 |
| Sequence similarities | Belongs to the peroxidase family. Peroxidase/catalase subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Hydrogen peroxide |
| Ligand | Heme Iron Metal-binding |
| Molecular function | Oxidoreductase Peroxidase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | catalase activity Inferred from electronic annotation. Source: HAMAP heme bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 726 | 726 | Catalase-peroxidase 1 HAMAP MF_01961 | PRO_0000354781 | |||||||
Sites | |||||||||||
| Active site | 106 | 1 | Proton acceptor By similarity | ||||||||
| Metal binding | 267 | 1 | Iron (heme axial ligand) By similarity | ||||||||
| Site | 102 | 1 | Transition state stabilizer By similarity | ||||||||
Amino acid modifications | |||||||||||
| Cross-link | 105 ↔ 226 | Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-252) By similarity | |||||||||
| Cross-link | 226 ↔ 252 | Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-105) By similarity | |||||||||
Sequences
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References
| [1] | "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. Shinagawa H.DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC. |
| [2] | "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. Blattner F.R.Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BA000007 Genomic DNA. Translation: BAB38294.1. AE005174 Genomic DNA. Translation: AAG59143.1. |
| PIR | C86085. G91237. |
| RefSeq | NP_290579.1. NP_312898.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1U2L based on UniProtKB P13029. |
| SMR | Q7A978. Positions 30-725. |
| ModBase | Search... |
Protein family/group databases | |
| PeroxiBase | 2718. EcoH7CP01_Sakai. |
Genome annotation databases | |
| GeneID | 915019. 960189. |
| GenomeReviews | Gene locus Z5497 in contig AE005174_GR. Gene locus ECs4871 in contig BA000007_GR. |
| KEGG | ece:Z5497. ecs:ECs4871. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | HBG285610. |
Enzyme and pathway databases | |
| BioCyc | ECOL83334:ECS4871-MONOMER. |
Family and domain databases | |
| HAMAP | MF_01961. Catal-peroxid. [Tree] |
| InterPro | IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view] |
| Pfam | PF00141. peroxidase. 2 hits. [Graphical view] |
| PRINTS | PR00460. BPEROXIDASE. PR00458. PEROXIDASE. |
| TIGRFAMs | TIGR00198. cat_per_HPI. 1 hit. |
| PROSITE | PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | KATG1_ECO57 | ||||||||
| Accession | Primary (citable) accession number: Q7A978 Secondary accession number(s): Q8X765 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||

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