Reviewed, UniProtKB/Swiss-Prot Q7A827 (ISDI_STAAN)

Names and origin

Protein names

Recommended name:
    Heme-degrading monooxygenase isdI
    EC=1.14.99.3
Alternative name(s):
    Iron-regulated surface determinant isdI
    Iron-responsive surface determinant isdI
    Heme oxygenase

Gene names

Name:	isdI
Ordered Locus Names:	SA0160

Organism

Staphylococcus aureus (strain N315) [Complete proteome] [HAMAP]

Taxonomic identifier

158879 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	108 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron By similarity.
Catalytic activity	Heme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O. HAMAP MF_01272
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the antibiotic biosynthesis monooxygenase family. Heme-degrading monooxygenase isdG subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme binding Inferred from electronic annotation. Source: HAMAP heme oxygenase (decyclizing) activity Inferred from electronic annotation. Source: EC monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 108	108	Heme-degrading monooxygenase isdI HAMAP MF_01272		PRO_0000270092
Sites
	Metal binding	6	1	Iron Potential
	Metal binding	76	1	Iron (heme axial ligand) Potential
	Site	66	1	Transition state stabilizer Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7A827-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 8AF2718571451004	FASTA	108	12,791
	10 20 30 40 50 60 MFMAENRLQL QKGSAEETIE RFYNRQGIET IEGFQQMFVT KTLNTEDTDE VKILTIWESE 70 80 90 100 DSFNNWLNSD VFKEAHKNVR LKSDDDGQQS PILSNKVFKY DIGYHYQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Shotgun proteomic analysis of total and membrane protein extracts of S. aureus strain N315." Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F. Submitted (OCT-2007) to UniProtKB Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

BA000018 Genomic DNA. Translation: BAB41380.1.

PIR

A89778.

RefSeq

NP_373402.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2ZDP	X-ray	1.50	A/B	1-108	[»]

SMR

Q7A827. Positions 1-108.

ModBase

Search...

Genome annotation databases

GeneID

1122935.

GenomeReviews

Gene locus SA0160 in contig BA000018_GR.

KEGG

sau:SA0160.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

Q7A827.

OMA

Q7A827. DAHSHQG.

Enzyme and pathway databases

BioCyc

SAUR158879:SA0160-MON.

Family and domain databases

HAMAP

MF_01272.
[Tree]

InterPro

IPR007138. Antibiotic_mOase.
[Graphical view]

Pfam

PF03992. ABM. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ISDI_STAAN

Accession

Primary (citable) accession number: Q7A827

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)