Q7A7L2 (THLA_STAAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable acetyl-CoA acyltransferase
EC=2.3.1.9

Alternative name(s):
Acetoacetyl-CoA thiolase

Gene names

Ordered Locus Names:

SA0342

Organism

Staphylococcus aureus (strain N315) [Complete proteome] [HAMAP]

Taxonomic identifier

158879 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	2 acetyl-CoA = CoA + acetoacetyl-CoA.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA C-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Probable acetyl-CoA acyltransferase		PRO_0000270505
Sites
	Active site	88	1	Acyl-thioester intermediate By similarity
	Active site	349	1	Proton acceptor By similarity
	Active site	378	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7A7L2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: EA0563681A298EFB	FASTA	393	41,753
	10 20 30 40 50 60 MTRVVLAAAY RTPIGVFGGA FKDVPAYDLG ATLIEHIIKE TGLNPSEIDE VIIGNVLQAG 70 80 90 100 110 120 QGQNPARIAA MKGGLPETVP AFTVNKVCGS GLKSIQLAYQ SIVTGENDIV LAGGMENMSQ 130 140 150 160 170 180 SPMLVNNSRF GFKMGHQSMV DSMVYDGLTD VFNQYHMGIT AENLVEQYGI SREEQDTFAV 190 200 210 220 230 240 NSQHKAVRAQ QNGEFDSEIV PVSIPQRKGE PILVTKDEGV RENVSVEKLS RLRPAFKKDG 250 260 270 280 290 300 TVTAGNASGI NDGAAMMLVM SEDKAKELNI EPLAVLDGFG SHGVDPSIMG IAPVGAVEKA 310 320 330 340 350 360 LKRSKKELSD IDVFELNEAF AAQLLAVDRE LKLPPEKVNV KGGAIALGHP IGASGARVLV 370 380 390 TLLHQLNDEV ETGLTSLCIG GGQAIAAVVS KYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: N315.
[2]	"Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth." Scherl A., Francois P., Bento M., Deshusses J.M., Charbonnier Y., Converset V., Huyghe A., Walter N., Hoogland C., Appel R.D., Sanchez J.-C., Zimmermann-Ivol C.G., Corthals G.L., Hochstrasser D.F., Schrenzel J. J. Microbiol. Methods 60:247-257(2005) [PubMed: 15590099] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: N315.
[3]	"Shotgun proteomic analysis of total and membrane protein extracts of S. aureus strain N315." Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F. Submitted (OCT-2007) to UniProtKB Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: N315.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000018 Genomic DNA. Translation: BAB41566.1.
PIR	C89801.
RefSeq	NP_373588.1. NC_002745.2.
3D structure databases
HSSP	HSSP built from PDB template 1WL4 based on UniProtKB Q9BWD1.
ProteinModelPortal	Q7A7L2.
SMR	Q7A7L2. Positions 4-390.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7A7L2.
2D gel databases
SWISS-2DPAGE	Q7A7L2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000001047; EBSTAP00000001047; EBSTAG00000001047.
GeneID	1123121.
GenomeReviews	Gene locus SA0342 in contig BA000018_GR.
KEGG	sau:SA0342.
PATRIC	19572738. VBIStaAur116463_0358.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0183.
GeneTree	EBGT00050000024085.
HOGENOM	HBG370930.
OMA	NPSEINE.
PhylomeDB	Q7A7L2.
ProtClustDB	CLSK884583.
Enzyme and pathway databases
BioCyc	SAUR158879:SA0342-MONOMER.
Family and domain databases
InterPro	IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020615. Thiolase_acyl_enz_int_AS. IPR020610. Thiolase_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 4 hits.
KO	K00626.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THLA_STAAN

Accession

Primary (citable) accession number: Q7A7L2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program