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Protein

Thermonuclease

Gene

nuc

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond.By similarity

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.PROSITE-ProRule annotation

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi100 – 1001CalciumPROSITE-ProRule annotation
Active sitei114 – 1141By similarity
Metal bindingi119 – 1191CalciumPROSITE-ProRule annotation
Metal bindingi120 – 1201Calcium; via carbonyl oxygenPROSITE-ProRule annotation
Active sitei122 – 1221By similarity
Active sitei166 – 1661By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciSAUR158879:GJCB-784-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Thermonuclease (EC:3.1.31.1)
Short name:
TNase
Alternative name(s):
Micrococcal nuclease
Staphylococcal nuclease
Gene namesi
Name:nuc
Ordered Locus Names:SA0746
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000751 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Propeptidei24 – 6037By similarityPRO_0000045231Add
BLAST
Chaini61 – 228168ThermonucleasePRO_0000045232Add
BLAST

Keywords - PTMi

Zymogen

Structurei

3D structure databases

ProteinModelPortaliQ7A6P2.
SMRiQ7A6P2. Positions 80-228.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thermonuclease family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000017398.
OMAiNEMTFIG.

Family and domain databases

Gene3Di2.40.50.90. 1 hit.
InterProiIPR016071. Staphylococal_nuclease_OB-fold.
IPR002071. Thermonucl_AS.
[Graphical view]
PfamiPF00565. SNase. 1 hit.
[Graphical view]
SMARTiSM00318. SNc. 1 hit.
[Graphical view]
SUPFAMiSSF50199. SSF50199. 1 hit.
PROSITEiPS01123. TNASE_1. 1 hit.
PS01284. TNASE_2. 1 hit.
PS50830. TNASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7A6P2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEYLLSAGI CMAIVSILLI GMAISNVSKG QYAKRFFFFA TSCLVLTLVV
60 70 80 90 100
VSSLSSSANA SQTDNGVNRS GSEDPTVYSA TSTKKLHKEP ATLIKAIDGD
110 120 130 140 150
TVKLMYKGQP MTFRLLLVDT PETKHPKKGV EKYGPEASAF TKKMVENANK
160 170 180 190 200
IEVEFDKGQR TDKYGRGLAY IYADGKMVNE ALVRQGLAKV AYVYKPNNTH
210 220
EQLLRKSEAQ AKKEKLNIWS EDNADSGQ
Length:228
Mass (Da):25,090
Last modified:July 5, 2004 - v1
Checksum:i6F288E1F368D8FF8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB41979.1.
PIRiH89852.
RefSeqiWP_000141557.1. NC_002745.2.

Genome annotation databases

EnsemblBacteriaiBAB41979; BAB41979; BAB41979.
PATRICi19573638. VBIStaAur116463_0790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB41979.1.
PIRiH89852.
RefSeqiWP_000141557.1. NC_002745.2.

3D structure databases

ProteinModelPortaliQ7A6P2.
SMRiQ7A6P2. Positions 80-228.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB41979; BAB41979; BAB41979.
PATRICi19573638. VBIStaAur116463_0790.

Phylogenomic databases

HOGENOMiHOG000017398.
OMAiNEMTFIG.

Enzyme and pathway databases

BioCyciSAUR158879:GJCB-784-MONOMER.

Family and domain databases

Gene3Di2.40.50.90. 1 hit.
InterProiIPR016071. Staphylococal_nuclease_OB-fold.
IPR002071. Thermonucl_AS.
[Graphical view]
PfamiPF00565. SNase. 1 hit.
[Graphical view]
SMARTiSM00318. SNc. 1 hit.
[Graphical view]
SUPFAMiSSF50199. SSF50199. 1 hit.
PROSITEiPS01123. TNASE_1. 1 hit.
PS01284. TNASE_2. 1 hit.
PS50830. TNASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUC_STAAN
AccessioniPrimary (citable) accession number: Q7A6P2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.