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Protein

Thermonuclease

Gene

nuc

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond.By similarity

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.PROSITE-ProRule annotation

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi100CalciumPROSITE-ProRule annotation1
Active sitei114By similarity1
Metal bindingi119CalciumPROSITE-ProRule annotation1
Metal bindingi120Calcium; via carbonyl oxygenPROSITE-ProRule annotation1
Active sitei122By similarity1
Active sitei166By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thermonuclease (EC:3.1.31.1)
Short name:
TNase
Alternative name(s):
Micrococcal nuclease
Staphylococcal nuclease
Gene namesi
Name:nuc
Ordered Locus Names:SA0746
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000751 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
PropeptideiPRO_000004523124 – 60By similarityAdd BLAST37
ChainiPRO_000004523261 – 228ThermonucleaseAdd BLAST168

Keywords - PTMi

Zymogen

Structurei

3D structure databases

ProteinModelPortaliQ7A6P2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thermonuclease family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000017398.
KOiK01174.
OMAiNEMTFIG.

Family and domain databases

Gene3Di2.40.50.90. 1 hit.
InterProiIPR016071. Staphylococal_nuclease_OB-fold.
IPR002071. Thermonucl_AS.
[Graphical view]
PfamiPF00565. SNase. 1 hit.
[Graphical view]
SMARTiSM00318. SNc. 1 hit.
[Graphical view]
SUPFAMiSSF50199. SSF50199. 1 hit.
PROSITEiPS01123. TNASE_1. 1 hit.
PS01284. TNASE_2. 1 hit.
PS50830. TNASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7A6P2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEYLLSAGI CMAIVSILLI GMAISNVSKG QYAKRFFFFA TSCLVLTLVV
60 70 80 90 100
VSSLSSSANA SQTDNGVNRS GSEDPTVYSA TSTKKLHKEP ATLIKAIDGD
110 120 130 140 150
TVKLMYKGQP MTFRLLLVDT PETKHPKKGV EKYGPEASAF TKKMVENANK
160 170 180 190 200
IEVEFDKGQR TDKYGRGLAY IYADGKMVNE ALVRQGLAKV AYVYKPNNTH
210 220
EQLLRKSEAQ AKKEKLNIWS EDNADSGQ
Length:228
Mass (Da):25,090
Last modified:July 5, 2004 - v1
Checksum:i6F288E1F368D8FF8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB41979.1.
PIRiH89852.
RefSeqiWP_000141557.1. NC_002745.2.

Genome annotation databases

EnsemblBacteriaiBAB41979; BAB41979; BAB41979.
KEGGisau:SA0746.
PATRICi19573638. VBIStaAur116463_0790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB41979.1.
PIRiH89852.
RefSeqiWP_000141557.1. NC_002745.2.

3D structure databases

ProteinModelPortaliQ7A6P2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB41979; BAB41979; BAB41979.
KEGGisau:SA0746.
PATRICi19573638. VBIStaAur116463_0790.

Phylogenomic databases

HOGENOMiHOG000017398.
KOiK01174.
OMAiNEMTFIG.

Family and domain databases

Gene3Di2.40.50.90. 1 hit.
InterProiIPR016071. Staphylococal_nuclease_OB-fold.
IPR002071. Thermonucl_AS.
[Graphical view]
PfamiPF00565. SNase. 1 hit.
[Graphical view]
SMARTiSM00318. SNc. 1 hit.
[Graphical view]
SUPFAMiSSF50199. SSF50199. 1 hit.
PROSITEiPS01123. TNASE_1. 1 hit.
PS01284. TNASE_2. 1 hit.
PS50830. TNASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUC_STAAN
AccessioniPrimary (citable) accession number: Q7A6P2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.