Reviewed, UniProtKB/Swiss-Prot Q7A695 (PURK_STAAN)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole carboxylase ATPase subunit
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC

Gene names

Name:	purK
Ordered Locus Names:	SA0917

Organism

Staphylococcus aureus (strain N315) [Complete proteome] [HAMAP]

Taxonomic identifier

158879 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Possesses an ATPase activity that is dependent on the presence of AIR (aminoimidazole ribonucleotide). The association of purK and purE produces an enzyme complex capable of converting AIR to CAIR efficiently under physiological condition By similarity.
Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purK/purT family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Phosphoribosylaminoimidazole carboxylase ATPase subunit		PRO_0000075005
Regions
	Domain	112 – 296	185	ATP-grasp
	Nucleotide binding	183 – 186	4	ATP By similarity
	Nucleotide binding	266 – 267	2	ATP By similarity
Sites
	Binding site	108	1	ATP By similarity
	Binding site	148	1	ATP By similarity
	Binding site	159	1	ATP By similarity
	Binding site	191	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7A695-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: B26CF7FDAEB426A0	FASTA	374	42,421
	10 20 30 40 50 60 MNFNKLKFGA TIGIIGGGQL GKMMAQSAQK MGYKVVVLDP SEDCPCRYVA HEFIQAKYDD 70 80 90 100 110 120 EKALNQLGQK CDVITYEFEN ISAQQLKLLC EKYNIPQGYQ AIQLLQDRLT EKETLKSAGT 130 140 150 160 170 180 KVVPFISVKE STDIDKAIET LGYPFIVKTR FGGYDGKGQV LINNEKDLQE GFKLIETSEC 190 200 210 220 230 240 VAEKYLNIKK EVSLTVTRGN NNQITFFPLQ ENEHRNQILF KTIVPARIDK TAEAKEQVNK 250 260 270 280 290 300 IIQSIHFIGT FTVEFFIDSN NQLYVNEIAP RPHNSGHYSI EACDYSQFDT HILAVTGQSL 310 320 330 340 350 360 PNSIELLKPA VMMNLLGKDL DLLENEFNEH PEWHLHIYGK SGRKDSRKMG HMTVLTNDVN 370 QTEQDMYAKF EGSN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Shotgun proteomic analysis of total and membrane protein extracts of S. aureus strain N315." Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F. Submitted (OCT-2007) to UniProtKB Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000018 Genomic DNA. Translation: BAB42162.1.
PIR	G89875.
RefSeq	NP_374184.1.
3D structure databases
HSSP	HSSP built from PDB template 1B6S based on UniProtKB P09029.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7A695.
Genome annotation databases
GeneID	1123740.
GenomeReviews	Gene locus SA0917 in contig BA000018_GR.
KEGG	sau:SA0917.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7A695.
OMA	RKIGHVN.
Enzyme and pathway databases
BioCyc	SAUR158879:SA0917-MON.
Family and domain databases
InterPro	IPR005875. AIR_COase_ATPase-su. IPR011761. ATP-grasp. IPR003135. ATP-grasp_carboxylate-amine. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PANTHER	PTHR23047:SF1. PurK_ATP. 1 hit.
Pfam	PF02222. ATP-grasp. 1 hit. [Graphical view]
TIGRFAMs	TIGR01161. purK. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURK_STAAN

Accession

Primary (citable) accession number: Q7A695

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)