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Protein

High-affinity heme uptake system protein IsdE

Gene

isdE

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in heme (porphyrin) scavenging. Binds Fe2+ and Fe3+ heme but the largest fraction is Fe2+ heme. Functions as a high-affinity heme binding protein and probably has a role in relaying heme-iron from cell wall-anchored isd proteins receptors to the probable permease IsdF (By similarity).By similarity

Cofactori

heme b1 PublicationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411Heme; via amide nitrogen1 Publication
Binding sitei42 – 421Heme; via amide nitrogen1 Publication
Binding sitei60 – 601Heme1 Publication
Binding sitei61 – 611Heme; via amide nitrogen1 Publication
Metal bindingi78 – 781Iron (heme axial ligand)
Metal bindingi229 – 2291Iron (heme axial ligand)

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciSAUR158879:GJCB-1036-MONOMER.

Protein family/group databases

TCDBi3.A.1.14.17. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
High-affinity heme uptake system protein IsdE
Alternative name(s):
Iron-regulated surface determinant protein E
Staphylococcal iron-regulated protein F
Gene namesi
Name:isdE
Synonyms:sirF
Ordered Locus Names:SA0980
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000751 Componenti: Chromosome

Subcellular locationi

  • Cell membrane PROSITE-ProRule annotation; Lipid-anchor PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919PROSITE-ProRule annotationAdd
BLAST
Chaini20 – 292273High-affinity heme uptake system protein IsdEPRO_0000326214Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi20 – 201N-palmitoyl cysteinePROSITE-ProRule annotation
Lipidationi20 – 201S-diacylglycerol cysteinePROSITE-ProRule annotation

Keywords - PTMi

Lipoprotein, Palmitate

Expressioni

Inductioni

Repressed by fur in the presence of iron.By similarity

Structurei

Secondary structure

1
292
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi36 – 383Combined sources
Helixi41 – 499Combined sources
Helixi66 – 683Combined sources
Helixi82 – 876Combined sources
Beta strandi91 – 966Combined sources
Helixi97 – 993Combined sources
Helixi100 – 11011Combined sources
Beta strandi115 – 1173Combined sources
Helixi122 – 13514Combined sources
Helixi139 – 16224Combined sources
Beta strandi168 – 1758Combined sources
Beta strandi178 – 1825Combined sources
Helixi187 – 1948Combined sources
Beta strandi198 – 2003Combined sources
Beta strandi205 – 2106Combined sources
Turni211 – 2133Combined sources
Beta strandi222 – 2287Combined sources
Helixi232 – 24514Combined sources
Helixi247 – 2515Combined sources
Helixi253 – 2564Combined sources
Beta strandi260 – 2623Combined sources
Turni265 – 2673Combined sources
Helixi276 – 28712Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2Q8PX-ray1.95A32-289[»]
2Q8QX-ray2.15A32-289[»]
ProteinModelPortaliQ7A652.
SMRiQ7A652. Positions 32-289.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7A652.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 291257Fe/B12 periplasmic-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Fe/B12 periplasmic-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000059045.
OMAiHFNAVKN.

Family and domain databases

InterProiIPR019957. ABC_transptr_haem-bd_IsdE.
IPR002491. ABC_transptr_periplasmic_BD.
[Graphical view]
PfamiPF01497. Peripla_BP_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03659. IsdE. 1 hit.
PROSITEiPS50983. FE_B12_PBP. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7A652-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIIKYLTIL VISVVILTSC QSSSSQESTK SGEFRIVPTT VALTMTLDKL
60 70 80 90 100
DLPIVGKPTS YKTLPNRYKD VPEIGQPMEP NVEAVKKLKP THVLSVSTIK
110 120 130 140 150
DEMQPFYKQL NMKGYFYDFD SLKGMQKSIT QLGDQFNRKA QAKELNDHLN
160 170 180 190 200
SVKQKIENKA AKQKKHPKVL ILMGVPGSYL VATDKSYIGD LVKIAGGENV
210 220 230 240 250
IKVKDRQYIS SNTENLLNIN PDIILRLPHG MPEEVKKMFQ KEFKQNDIWK
260 270 280 290
HFKAVKNNHV YDLEEVPFGI TANVDADKAM TQLYDLFYKD KK
Length:292
Mass (Da):33,271
Last modified:July 5, 2004 - v1
Checksum:i62E301DE778458F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB42229.1.
PIRiA89884.
RefSeqiWP_001220199.1. NC_002745.2.

Genome annotation databases

EnsemblBacteriaiBAB42229; BAB42229; BAB42229.
PATRICi19574156. VBIStaAur116463_1048.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB42229.1.
PIRiA89884.
RefSeqiWP_001220199.1. NC_002745.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2Q8PX-ray1.95A32-289[»]
2Q8QX-ray2.15A32-289[»]
ProteinModelPortaliQ7A652.
SMRiQ7A652. Positions 32-289.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi3.A.1.14.17. the atp-binding cassette (abc) superfamily.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB42229; BAB42229; BAB42229.
PATRICi19574156. VBIStaAur116463_1048.

Phylogenomic databases

HOGENOMiHOG000059045.
OMAiHFNAVKN.

Enzyme and pathway databases

BioCyciSAUR158879:GJCB-1036-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ7A652.

Family and domain databases

InterProiIPR019957. ABC_transptr_haem-bd_IsdE.
IPR002491. ABC_transptr_periplasmic_BD.
[Graphical view]
PfamiPF01497. Peripla_BP_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03659. IsdE. 1 hit.
PROSITEiPS50983. FE_B12_PBP. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiISDE_STAAN
AccessioniPrimary (citable) accession number: Q7A652
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.