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Protein

High-affinity heme uptake system protein IsdE

Gene

isdE

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in heme (porphyrin) scavenging. Binds Fe2+ and Fe3+ heme but the largest fraction is Fe2+ heme. Functions as a high-affinity heme binding protein and probably has a role in relaying heme-iron from cell wall-anchored isd proteins receptors to the probable permease IsdF (By similarity).By similarity

Cofactori

heme b1 PublicationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41Heme; via amide nitrogen1 Publication1
Binding sitei42Heme; via amide nitrogen1 Publication1
Binding sitei60Heme1 Publication1
Binding sitei61Heme; via amide nitrogen1 Publication1
Metal bindingi78Iron (heme axial ligand)1
Metal bindingi229Iron (heme axial ligand)1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Protein family/group databases

TCDBi3.A.1.14.17. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
High-affinity heme uptake system protein IsdE
Alternative name(s):
Iron-regulated surface determinant protein E
Staphylococcal iron-regulated protein F
Gene namesi
Name:isdE
Synonyms:sirF
Ordered Locus Names:SA0980
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000751 Componenti: Chromosome

Subcellular locationi

  • Cell membrane PROSITE-ProRule annotation; Lipid-anchor PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19PROSITE-ProRule annotationAdd BLAST19
ChainiPRO_000032621420 – 292High-affinity heme uptake system protein IsdEAdd BLAST273

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi20N-palmitoyl cysteinePROSITE-ProRule annotation1
Lipidationi20S-diacylglycerol cysteinePROSITE-ProRule annotation1

Keywords - PTMi

Lipoprotein, Palmitate

Expressioni

Inductioni

Repressed by fur in the presence of iron.By similarity

Structurei

Secondary structure

1292
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi36 – 38Combined sources3
Helixi41 – 49Combined sources9
Helixi66 – 68Combined sources3
Helixi82 – 87Combined sources6
Beta strandi91 – 96Combined sources6
Helixi97 – 99Combined sources3
Helixi100 – 110Combined sources11
Beta strandi115 – 117Combined sources3
Helixi122 – 135Combined sources14
Helixi139 – 162Combined sources24
Beta strandi168 – 175Combined sources8
Beta strandi178 – 182Combined sources5
Helixi187 – 194Combined sources8
Beta strandi198 – 200Combined sources3
Beta strandi205 – 210Combined sources6
Turni211 – 213Combined sources3
Beta strandi222 – 228Combined sources7
Helixi232 – 245Combined sources14
Helixi247 – 251Combined sources5
Helixi253 – 256Combined sources4
Beta strandi260 – 262Combined sources3
Turni265 – 267Combined sources3
Helixi276 – 287Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Q8PX-ray1.95A32-289[»]
2Q8QX-ray2.15A32-289[»]
ProteinModelPortaliQ7A652.
SMRiQ7A652.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7A652.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 291Fe/B12 periplasmic-bindingPROSITE-ProRule annotationAdd BLAST257

Sequence similaritiesi

Contains 1 Fe/B12 periplasmic-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000059045.
KOiK02016.
OMAiHFNAVKN.

Family and domain databases

InterProiIPR019957. ABC_transptr_haem-bd_IsdE.
IPR002491. ABC_transptr_periplasmic_BD.
[Graphical view]
PfamiPF01497. Peripla_BP_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03659. IsdE. 1 hit.
PROSITEiPS50983. FE_B12_PBP. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7A652-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIIKYLTIL VISVVILTSC QSSSSQESTK SGEFRIVPTT VALTMTLDKL
60 70 80 90 100
DLPIVGKPTS YKTLPNRYKD VPEIGQPMEP NVEAVKKLKP THVLSVSTIK
110 120 130 140 150
DEMQPFYKQL NMKGYFYDFD SLKGMQKSIT QLGDQFNRKA QAKELNDHLN
160 170 180 190 200
SVKQKIENKA AKQKKHPKVL ILMGVPGSYL VATDKSYIGD LVKIAGGENV
210 220 230 240 250
IKVKDRQYIS SNTENLLNIN PDIILRLPHG MPEEVKKMFQ KEFKQNDIWK
260 270 280 290
HFKAVKNNHV YDLEEVPFGI TANVDADKAM TQLYDLFYKD KK
Length:292
Mass (Da):33,271
Last modified:July 5, 2004 - v1
Checksum:i62E301DE778458F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB42229.1.
PIRiA89884.
RefSeqiWP_001220199.1. NC_002745.2.

Genome annotation databases

EnsemblBacteriaiBAB42229; BAB42229; BAB42229.
GeneIDi28381316.
KEGGisau:SA0980.
PATRICi19574156. VBIStaAur116463_1048.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB42229.1.
PIRiA89884.
RefSeqiWP_001220199.1. NC_002745.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Q8PX-ray1.95A32-289[»]
2Q8QX-ray2.15A32-289[»]
ProteinModelPortaliQ7A652.
SMRiQ7A652.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi3.A.1.14.17. the atp-binding cassette (abc) superfamily.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB42229; BAB42229; BAB42229.
GeneIDi28381316.
KEGGisau:SA0980.
PATRICi19574156. VBIStaAur116463_1048.

Phylogenomic databases

HOGENOMiHOG000059045.
KOiK02016.
OMAiHFNAVKN.

Miscellaneous databases

EvolutionaryTraceiQ7A652.

Family and domain databases

InterProiIPR019957. ABC_transptr_haem-bd_IsdE.
IPR002491. ABC_transptr_periplasmic_BD.
[Graphical view]
PfamiPF01497. Peripla_BP_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03659. IsdE. 1 hit.
PROSITEiPS50983. FE_B12_PBP. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiISDE_STAAN
AccessioniPrimary (citable) accession number: Q7A652
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.