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Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

Catalytic activityi

N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + reduced electron acceptor = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi77Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi113Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi147Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi223Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi227Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi230Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Ordered Locus Names:SA1134
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000751 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003745731 – 514tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseAdd BLAST514

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ7A5W3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 186MTTase N-terminalUniRule annotationAdd BLAST119
Domaini442 – 505TRAMUniRule annotationAdd BLAST64

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation
Contains 1 MTTase N-terminal domain.UniRule annotation
Contains 1 TRAM domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000224767.
KOiK06168.
OMAiCQMNFSD.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7A5W3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEEQRKASS VDVLAERDKK AEKDYSKYFE HVYQPPNLKE AKKRGKQEVR
60 70 80 90 100
YNRDFQIDEK YRGMGNERTF LIKTYGCQMN AHDTEVIAGI LEALGYQATT
110 120 130 140 150
DINTADVILI NTCAIRENAE NKVFSEIGNL KHLKKERPDI LIGVCGCMSQ
160 170 180 190 200
EESVVNKILK SYQNVDMIFG THNIHHLPEI LEEAYLSKAM VVEVWSKEGD
210 220 230 240 250
VIENLPKVRE GNIKAWVNIM YGCDKFCTYC IVPFTRGKER SRRPEDIIDE
260 270 280 290 300
VRELAREGYK EITLLGQNVN SYGKDLQDIE YDLGDLLQAI SKIAIPRVRF
310 320 330 340 350
TTSHPWDFTD HMIDVISEGG NIVPHIHLPV QSGNNAVLKI MGRKYTRESY
360 370 380 390 400
LDLVKRIKDR IPNVALTTDI IVGYPNESEE QFEETLTLYD EVGFEHAYTY
410 420 430 440 450
LYSQRDGTPA AKMKDNVPLN VKKERLQRLN KKVGHYSQIA MSKYEGQTVT
460 470 480 490 500
VLCEGSSKKD DQVLAGYTDK NKLVNFKAPK EMIGKLVEVR IDEAKQYSLN
510
GSFVKEVEPE MVIQ
Length:514
Mass (Da):58,917
Last modified:July 5, 2004 - v1
Checksum:i9E864C7A17B4EAD8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB42387.1.
PIRiG89903.
RefSeqiWP_001001524.1. NC_002745.2.

Genome annotation databases

EnsemblBacteriaiBAB42387; BAB42387; BAB42387.
KEGGisau:SA1134.
PATRICi19574490. VBIStaAur116463_1214.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB42387.1.
PIRiG89903.
RefSeqiWP_001001524.1. NC_002745.2.

3D structure databases

ProteinModelPortaliQ7A5W3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB42387; BAB42387; BAB42387.
KEGGisau:SA1134.
PATRICi19574490. VBIStaAur116463_1214.

Phylogenomic databases

HOGENOMiHOG000224767.
KOiK06168.
OMAiCQMNFSD.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB. 1 hit.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMIAB_STAAN
AccessioniPrimary (citable) accession number: Q7A5W3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.