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Protein

CCA-adding enzyme

Gene

cca

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.UniRule annotation

Catalytic activityi

A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei28 – 281ATP or CTP; via amide nitrogenUniRule annotation
Binding sitei31 – 311ATP or CTPUniRule annotation
Metal bindingi41 – 411MagnesiumUniRule annotation
Metal bindingi43 – 431MagnesiumUniRule annotation
Binding sitei112 – 1121ATP or CTPUniRule annotation
Binding sitei155 – 1551ATP or CTPUniRule annotation
Binding sitei158 – 1581ATP or CTPUniRule annotation
Binding sitei161 – 1611ATP or CTPUniRule annotation
Binding sitei164 – 1641ATP or CTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

RNA repair, tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciSAUR158879:GJCB-1358-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CCA-adding enzymeUniRule annotation (EC:2.7.7.72UniRule annotation)
Alternative name(s):
CCA tRNA nucleotidyltransferaseUniRule annotation
tRNA CCA-pyrophosphorylaseUniRule annotation
tRNA adenylyl-/cytidylyl- transferaseUniRule annotation
tRNA nucleotidyltransferaseUniRule annotation
tRNA-NTUniRule annotation
Gene namesi
Name:ccaUniRule annotation
Ordered Locus Names:SA1290
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000751 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400CCA-adding enzymePRO_0000139047Add
BLAST

Proteomic databases

PRIDEiQ7A5K2.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ7A5K2.
SMRiQ7A5K2. Positions 3-394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000253346.
OMAiERITVEW.
OrthoDBiEOG65QWJN.

Family and domain databases

HAMAPiMF_01263. CCA_bact_type3.
InterProiIPR032810. CCA-adding_enz_C.
IPR023068. CCA-adding_enz_firmicutes.
IPR002646. PolA_pol_head_dom.
IPR032828. PolyA_RNA-bd.
[Graphical view]
PfamiPF01743. PolyA_pol. 1 hit.
PF12627. PolyA_pol_RNAbd. 1 hit.
PF13735. tRNA_NucTran2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7A5K2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKSLFEQAR PILEQIQDNG FEAYYVGGSV RDYVMGRNIH DIDITTSATP
60 70 80 90 100
DEIESIFSHT IPVGKEHGTI NVVFNDENYE VTTFRAEEDY VDHRRPSGVT
110 120 130 140 150
FVRDLYEDLQ RRDFTMNAIA MDTAYKLYDY FDGQQDINNR IIRTVGIAEE
160 170 180 190 200
RFQEDALRMI RCLRFQSQLS FDIAMETFEA MRTQMADIKF LSIERIVIEL
210 220 230 240 250
TKLMRGINVE ESFNHLKSLK AFNYMPYFEQ LDMNQINVTE PIDLELLIAI
260 270 280 290 300
VSVKFDINYS LKPLKLSNRQ VKDINQYIQI MNALPSIITK EQLKMFVYDY
310 320 330 340 350
DTNLIKNVMV AADVLKANDI QGHEPLIVNL QTIDETLHRL PMHNRKDMMV
360 370 380 390 400
NGGVLMAHLN AKSGPWLKDV LRQIEIAIVT GKVSNEETEI LKWVDNHVKI
Length:400
Mass (Da):46,449
Last modified:July 5, 2004 - v1
Checksum:i2EC9320C6ECFCADA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB42550.1.
PIRiA89924.
RefSeqiWP_000361540.1. NC_002745.2.

Genome annotation databases

EnsemblBacteriaiBAB42550; BAB42550; BAB42550.
PATRICi19574840. VBIStaAur116463_1389.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB42550.1.
PIRiA89924.
RefSeqiWP_000361540.1. NC_002745.2.

3D structure databases

ProteinModelPortaliQ7A5K2.
SMRiQ7A5K2. Positions 3-394.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ7A5K2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB42550; BAB42550; BAB42550.
PATRICi19574840. VBIStaAur116463_1389.

Phylogenomic databases

HOGENOMiHOG000253346.
OMAiERITVEW.
OrthoDBiEOG65QWJN.

Enzyme and pathway databases

BioCyciSAUR158879:GJCB-1358-MONOMER.

Family and domain databases

HAMAPiMF_01263. CCA_bact_type3.
InterProiIPR032810. CCA-adding_enz_C.
IPR023068. CCA-adding_enz_firmicutes.
IPR002646. PolA_pol_head_dom.
IPR032828. PolyA_RNA-bd.
[Graphical view]
PfamiPF01743. PolyA_pol. 1 hit.
PF12627. PolyA_pol_RNAbd. 1 hit.
PF13735. tRNA_NucTran2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: N315.

Entry informationi

Entry nameiCCA_STAAN
AccessioniPrimary (citable) accession number: Q7A5K2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: July 5, 2004
Last modified: March 16, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

A single active site specifically recognizes both ATP and CTP and is responsible for their addition.UniRule annotation

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.