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Q7A455

- TOP3_STAAN

UniProt

Q7A455 - TOP3_STAAN

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Protein

DNA topoisomerase 3

Gene

topB

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Magnesium; catalyticUniRule annotation
Sitei60 – 601Interaction with DNAUniRule annotation
Metal bindingi104 – 1041Magnesium; catalyticUniRule annotation
Sitei167 – 1671Interaction with DNAUniRule annotation
Sitei175 – 1751Interaction with DNAUniRule annotation
Active sitei305 – 3051O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
Sitei307 – 3071Interaction with DNAUniRule annotation

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSAUR158879:GJCB-2204-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IIIUniRule annotation
Gene namesi
Name:topBUniRule annotation
Ordered Locus Names:SA2051
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000000751: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 711711DNA topoisomerase 3PRO_0000286372Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi158879.SA2051.

Structurei

3D structure databases

ProteinModelPortaliQ7A455.
SMRiQ7A455. Positions 1-640.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 135134ToprimUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni186 – 1916Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0551.
KOiK03169.
OMAiTKHDEYF.
OrthoDBiEOG60CWM6.

Family and domain databases

Gene3Di1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00953. Topoisom_3_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01056. topB. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7A455-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSLILAEKP SVARDIADAL QINQKRNGYF ENNQYIVTWA LGHLVTNATP
60 70 80 90 100
EQYDKNLKEW RLEDLPIIPK YMKTVVIGKT SKQFKTVKAL ILDNKVKDII
110 120 130 140 150
IATDAGREGE LVARLILDKV GNKKPIRRLW ISSVTKKAIQ QGFKNLKDGR
160 170 180 190 200
QYNDLYYAAL ARSEADWIVG INATRALTTK YDAQLSLGRV QTPTIQLVNT
210 220 230 240 250
RQQEINQFKP QQYFTLSLTV KGFDFQLESN QRYTNKETLE QMVNNLKNVD
260 270 280 290 300
GKIKSVATKH KKSYPQSLYN LTDLQQDMYR RYKIGPKETL NTLQSLYERH
310 320 330 340 350
KVVTYPRTDS NYLTTDMVDT MKERIQATMA TTYKDQARPL MSKTFSSKMS
360 370 380 390 400
IFNNQKVSDH HAIIPTEVRP VMSDLSNREL KLYDMIVERF LEALMPPHEY
410 420 430 440 450
DAITVTLEVA GHTFVLKENV TTVLGFKSIR QGESITEMQQ PFSEGDEVKI
460 470 480 490 500
SKTNIREHET TPPEYFNEGS LLKAMENPQN FIQLKDKKYA QTLKQTGGIG
510 520 530 540 550
TVATRADIID KLFNMNAIES RDGKIKVTSK GKQILELAPE ELTSPLLTAQ
560 570 580 590 600
WEEKLLLIER GKYQAKTFIN EMKDFTKDVV NGIKNSDRKY KHDNLTTTEC
610 620 630 640 650
PTCGKFMIKV KTKNGQMLVC QDPSCKTKKN VQRKTNARCP NCKKKLTLFG
660 670 680 690 700
KGKEAVYRCV CGHSETQAHM DQRMKSKSSG KVSRKEMKKY MNKNEGLDNN
710
PFKDALKNLN L
Length:711
Mass (Da):81,524
Last modified:July 5, 2004 - v1
Checksum:i9C67B5BAC841CBB6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000018 Genomic DNA. Translation: BAB43346.1.
PIRiA90023.
RefSeqiNP_375367.1. NC_002745.2.

Genome annotation databases

EnsemblBacteriaiBAB43346; BAB43346; BAB43346.
GeneIDi1124972.
KEGGisau:SA2051.
PATRICi19576604. VBIStaAur116463_2220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000018 Genomic DNA. Translation: BAB43346.1 .
PIRi A90023.
RefSeqi NP_375367.1. NC_002745.2.

3D structure databases

ProteinModelPortali Q7A455.
SMRi Q7A455. Positions 1-640.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 158879.SA2051.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB43346 ; BAB43346 ; BAB43346 .
GeneIDi 1124972.
KEGGi sau:SA2051.
PATRICi 19576604. VBIStaAur116463_2220.

Phylogenomic databases

eggNOGi COG0551.
KOi K03169.
OMAi TKHDEYF.
OrthoDBi EOG60CWM6.

Enzyme and pathway databases

BioCyci SAUR158879:GJCB-2204-MONOMER.

Family and domain databases

Gene3Di 1.10.290.10. 1 hit.
1.10.460.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00953. Topoisom_3_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013826. Topo_IA_cen_sub3.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01056. topB. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: N315.

Entry informationi

Entry nameiTOP3_STAAN
AccessioniPrimary (citable) accession number: Q7A455
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3