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Q7A3G4

- PTU3C_STAAN

UniProt

Q7A3G4 - PTU3C_STAAN

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Protein
PTS system glucoside-specific EIICBA component
Gene
glcB, SA2326
Organism
Staphylococcus aureus (strain N315)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in alpha- and beta-glucoside transport By similarity.

Catalytic activityi

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei460 – 4601Phosphocysteine intermediate; for EIIB activity By similarity
Active sitei612 – 6121Tele-phosphohistidine intermediate; for EIIA activity By similarity

GO - Molecular functioni

  1. glucose transmembrane transporter activity Source: InterPro
  2. kinase activity Source: UniProtKB-KW
  3. protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciSAUR158879:GJCB-2487-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system glucoside-specific EIICBA component
Including the following 3 domains:
Glucoside permease IIC component
Alternative name(s):
PTS system glucoside-specific EIIC component
Glucoside-specific phosphotransferase enzyme IIB component (EC:2.7.1.69)
Alternative name(s):
PTS system glucoside-specific EIIB component
Glucoside-specific phosphotransferase enzyme IIA component (EC:2.7.1.-)
Alternative name(s):
PTS system glucoside-specific EIIA component
Gene namesi
Name:glcB
Ordered Locus Names:SA2326
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000000751: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei12 – 3221Helical; Reviewed prediction
Add
BLAST
Transmembranei81 – 10121Helical; Reviewed prediction
Add
BLAST
Transmembranei137 – 15721Helical; Reviewed prediction
Add
BLAST
Transmembranei182 – 20221Helical; Reviewed prediction
Add
BLAST
Transmembranei223 – 24321Helical; Reviewed prediction
Add
BLAST
Transmembranei284 – 30421Helical; Reviewed prediction
Add
BLAST
Transmembranei315 – 33521Helical; Reviewed prediction
Add
BLAST
Transmembranei340 – 36021Helical; Reviewed prediction
Add
BLAST
Transmembranei364 – 38421Helical; Reviewed prediction
Add
BLAST
Transmembranei395 – 41521Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 688688PTS system glucoside-specific EIICBA component
PRO_0000351414Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi158879.SA2326.

Structurei

3D structure databases

ProteinModelPortaliQ7A3G4.
SMRiQ7A3G4. Positions 447-509, 532-671.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 427425PTS EIIC type-1
Add
BLAST
Domaini438 – 51982PTS EIIB type-1
Add
BLAST
Domaini560 – 664105PTS EIIA type-1
Add
BLAST

Domaini

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1263.
HOGENOMiHOG000250993.
KOiK02763.
K02764.
K02765.
OMAiMQIGEFT.
OrthoDBiEOG6FFS9V.

Family and domain databases

Gene3Di3.30.1360.60. 1 hit.
InterProiIPR011055. Dup_hybrid_motif.
IPR018113. PTrfase_EIIB_Cys.
IPR001127. PTS_EIIA_1_perm.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
IPR011299. PTS_IIBC_glc.
[Graphical view]
PfamiPF00358. PTS_EIIA_1. 1 hit.
PF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMiSSF51261. SSF51261. 1 hit.
SSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
TIGR00830. PTBA. 1 hit.
TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITEiPS51093. PTS_EIIA_TYPE_1. 1 hit.
PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit.
PS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7A3G4-1 [UniParc]FASTAAdd to Basket

« Hide

MFKKLFGQLQ RIGKALMLPV AILPAAGILL AFGNAMHNEQ LVEIAPWLKN    50
DIIVMISSVM EAAGQVVFDN LPLLFAVGTA LGLAGGDGVA ALAALVGYLI 100
MNATMGKVLH ITIDDIFSYA KGAKELSQAA KEPAHALVLG IPTLQTGVFG 150
GIIMGALAAW CYNKFYNITL PPFLGFFAGK RFVPIVTSVV AIATGVLLSF 200
AWPPIQDGLN SLSNFLLNKN LTLTTFIFGI IERSLIPFGL HHIFYSPFWF 250
EFGSYTNHAG ELVRGDQRIW MAQLKDGVPF TAGAFTTGKY PFMMFGLPAA 300
AFAIYKNARP ERKKVVGGLM LSAGLTAFLT GITEPLEFSF LFVAPVLYGI 350
HVLLAGTSFL VMHLLGVKIG MTFSGGFIDY ILYGLLNWDR SHALLVIPVG 400
IVYAIVYYFL FDFAIRKFKL KTPGREDEET EIRNSSVAKL PFDVLDAMGG 450
KENIKHLDAC ITRLRVEVVD KSKVDVAGIK ALGASGVLEV GNNMQAIFGP 500
KSDQIKHDMA KIMSGEITKP SETTVTEEMS DEPVHVEALG TTDIYAPGVG 550
QIIPLSEVPD QVFAGKMMGD GIGFIPEKGE IVAPFDGTVK TIFPTKHAIG 600
LESESGVEVL IHIGIDTVKL NGEGFESLIN VDEKVTQAQP LMKVNLAYLK 650
AHAPSIVTPM IITNLENKEL VIEDVQDADP GKLIMTVK 688
Length:688
Mass (Da):74,430
Last modified:July 5, 2004 - v1
Checksum:i11D5C824835AFD21
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000018 Genomic DNA. Translation: BAB43629.1.
PIRiC90058.
RefSeqiNP_375650.1. NC_002745.2.

Genome annotation databases

EnsemblBacteriaiBAB43629; BAB43629; BAB43629.
GeneIDi1125253.
KEGGisau:SA2326.
PATRICi19577210. VBIStaAur116463_2523.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000018 Genomic DNA. Translation: BAB43629.1 .
PIRi C90058.
RefSeqi NP_375650.1. NC_002745.2.

3D structure databases

ProteinModelPortali Q7A3G4.
SMRi Q7A3G4. Positions 447-509, 532-671.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 158879.SA2326.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB43629 ; BAB43629 ; BAB43629 .
GeneIDi 1125253.
KEGGi sau:SA2326.
PATRICi 19577210. VBIStaAur116463_2523.

Phylogenomic databases

eggNOGi COG1263.
HOGENOMi HOG000250993.
KOi K02763.
K02764.
K02765.
OMAi MQIGEFT.
OrthoDBi EOG6FFS9V.

Enzyme and pathway databases

BioCyci SAUR158879:GJCB-2487-MONOMER.

Family and domain databases

Gene3Di 3.30.1360.60. 1 hit.
InterProi IPR011055. Dup_hybrid_motif.
IPR018113. PTrfase_EIIB_Cys.
IPR001127. PTS_EIIA_1_perm.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
IPR011299. PTS_IIBC_glc.
[Graphical view ]
Pfami PF00358. PTS_EIIA_1. 1 hit.
PF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view ]
SUPFAMi SSF51261. SSF51261. 1 hit.
SSF55604. SSF55604. 1 hit.
TIGRFAMsi TIGR00826. EIIB_glc. 1 hit.
TIGR00830. PTBA. 1 hit.
TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITEi PS51093. PTS_EIIA_TYPE_1. 1 hit.
PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit.
PS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: N315.
  2. "Shotgun proteomic analysis of total and membrane protein extracts of S. aureus strain N315."
    Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F.
    Submitted (OCT-2007) to UniProtKB
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: N315.

Entry informationi

Entry nameiPTU3C_STAAN
AccessioniPrimary (citable) accession number: Q7A3G4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: July 5, 2004
Last modified: September 3, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi