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Q7A3G4

- PTU3C_STAAN

UniProt

Q7A3G4 - PTU3C_STAAN

Protein

PTS system glucoside-specific EIICBA component

Gene

glcB

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in alpha- and beta-glucoside transport By similarity.By similarity

    Catalytic activityi

    Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.PROSITE-ProRule annotation
    Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei460 – 4601Phosphocysteine intermediate; for EIIB activityPROSITE-ProRule annotation
    Active sitei612 – 6121Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. glucose transmembrane transporter activity Source: InterPro
    2. kinase activity Source: UniProtKB-KW
    3. protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Phosphotransferase system, Sugar transport, Transport

    Enzyme and pathway databases

    BioCyciSAUR158879:GJCB-2487-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    PTS system glucoside-specific EIICBA component
    Including the following 3 domains:
    Glucoside permease IIC component
    Alternative name(s):
    PTS system glucoside-specific EIIC component
    Glucoside-specific phosphotransferase enzyme IIB component (EC:2.7.1.69)
    Alternative name(s):
    PTS system glucoside-specific EIIB component
    Glucoside-specific phosphotransferase enzyme IIA component (EC:2.7.1.-)
    Alternative name(s):
    PTS system glucoside-specific EIIA component
    Gene namesi
    Name:glcB
    Ordered Locus Names:SA2326
    OrganismiStaphylococcus aureus (strain N315)
    Taxonomic identifieri158879 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000000751: Chromosome

    Subcellular locationi

    Cell membrane PROSITE-ProRule annotation; Multi-pass membrane protein PROSITE-ProRule annotation

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 688688PTS system glucoside-specific EIICBA componentPRO_0000351414Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi158879.SA2326.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7A3G4.
    SMRiQ7A3G4. Positions 447-509, 532-671.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3221HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei81 – 10121HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei137 – 15721HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei182 – 20221HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei223 – 24321HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei284 – 30421HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei315 – 33521HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei340 – 36021HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei364 – 38421HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei395 – 41521HelicalPROSITE-ProRule annotationAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 427425PTS EIIC type-1PROSITE-ProRule annotationAdd
    BLAST
    Domaini438 – 51982PTS EIIB type-1PROSITE-ProRule annotationAdd
    BLAST
    Domaini560 – 664105PTS EIIA type-1PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
    The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
    The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.

    Sequence similaritiesi

    Contains 1 PTS EIIA type-1 domain.PROSITE-ProRule annotation
    Contains 1 PTS EIIB type-1 domain.PROSITE-ProRule annotation
    Contains 1 PTS EIIC type-1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1263.
    HOGENOMiHOG000250993.
    KOiK02763.
    K02764.
    K02765.
    OMAiMQIGEFT.
    OrthoDBiEOG6FFS9V.

    Family and domain databases

    Gene3Di3.30.1360.60. 1 hit.
    InterProiIPR011055. Dup_hybrid_motif.
    IPR018113. PTrfase_EIIB_Cys.
    IPR001127. PTS_EIIA_1_perm.
    IPR003352. PTS_EIIC.
    IPR013013. PTS_EIIC_1.
    IPR001996. PTS_IIB_1.
    IPR011299. PTS_IIBC_glc.
    [Graphical view]
    PfamiPF00358. PTS_EIIA_1. 1 hit.
    PF00367. PTS_EIIB. 1 hit.
    PF02378. PTS_EIIC. 1 hit.
    [Graphical view]
    SUPFAMiSSF51261. SSF51261. 1 hit.
    SSF55604. SSF55604. 1 hit.
    TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
    TIGR00830. PTBA. 1 hit.
    TIGR02002. PTS-II-BC-glcB. 1 hit.
    PROSITEiPS51093. PTS_EIIA_TYPE_1. 1 hit.
    PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit.
    PS51098. PTS_EIIB_TYPE_1. 1 hit.
    PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
    PS51103. PTS_EIIC_TYPE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7A3G4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFKKLFGQLQ RIGKALMLPV AILPAAGILL AFGNAMHNEQ LVEIAPWLKN    50
    DIIVMISSVM EAAGQVVFDN LPLLFAVGTA LGLAGGDGVA ALAALVGYLI 100
    MNATMGKVLH ITIDDIFSYA KGAKELSQAA KEPAHALVLG IPTLQTGVFG 150
    GIIMGALAAW CYNKFYNITL PPFLGFFAGK RFVPIVTSVV AIATGVLLSF 200
    AWPPIQDGLN SLSNFLLNKN LTLTTFIFGI IERSLIPFGL HHIFYSPFWF 250
    EFGSYTNHAG ELVRGDQRIW MAQLKDGVPF TAGAFTTGKY PFMMFGLPAA 300
    AFAIYKNARP ERKKVVGGLM LSAGLTAFLT GITEPLEFSF LFVAPVLYGI 350
    HVLLAGTSFL VMHLLGVKIG MTFSGGFIDY ILYGLLNWDR SHALLVIPVG 400
    IVYAIVYYFL FDFAIRKFKL KTPGREDEET EIRNSSVAKL PFDVLDAMGG 450
    KENIKHLDAC ITRLRVEVVD KSKVDVAGIK ALGASGVLEV GNNMQAIFGP 500
    KSDQIKHDMA KIMSGEITKP SETTVTEEMS DEPVHVEALG TTDIYAPGVG 550
    QIIPLSEVPD QVFAGKMMGD GIGFIPEKGE IVAPFDGTVK TIFPTKHAIG 600
    LESESGVEVL IHIGIDTVKL NGEGFESLIN VDEKVTQAQP LMKVNLAYLK 650
    AHAPSIVTPM IITNLENKEL VIEDVQDADP GKLIMTVK 688
    Length:688
    Mass (Da):74,430
    Last modified:July 5, 2004 - v1
    Checksum:i11D5C824835AFD21
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000018 Genomic DNA. Translation: BAB43629.1.
    PIRiC90058.
    RefSeqiNP_375650.1. NC_002745.2.

    Genome annotation databases

    EnsemblBacteriaiBAB43629; BAB43629; BAB43629.
    GeneIDi1125253.
    KEGGisau:SA2326.
    PATRICi19577210. VBIStaAur116463_2523.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000018 Genomic DNA. Translation: BAB43629.1 .
    PIRi C90058.
    RefSeqi NP_375650.1. NC_002745.2.

    3D structure databases

    ProteinModelPortali Q7A3G4.
    SMRi Q7A3G4. Positions 447-509, 532-671.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 158879.SA2326.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB43629 ; BAB43629 ; BAB43629 .
    GeneIDi 1125253.
    KEGGi sau:SA2326.
    PATRICi 19577210. VBIStaAur116463_2523.

    Phylogenomic databases

    eggNOGi COG1263.
    HOGENOMi HOG000250993.
    KOi K02763.
    K02764.
    K02765.
    OMAi MQIGEFT.
    OrthoDBi EOG6FFS9V.

    Enzyme and pathway databases

    BioCyci SAUR158879:GJCB-2487-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1360.60. 1 hit.
    InterProi IPR011055. Dup_hybrid_motif.
    IPR018113. PTrfase_EIIB_Cys.
    IPR001127. PTS_EIIA_1_perm.
    IPR003352. PTS_EIIC.
    IPR013013. PTS_EIIC_1.
    IPR001996. PTS_IIB_1.
    IPR011299. PTS_IIBC_glc.
    [Graphical view ]
    Pfami PF00358. PTS_EIIA_1. 1 hit.
    PF00367. PTS_EIIB. 1 hit.
    PF02378. PTS_EIIC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51261. SSF51261. 1 hit.
    SSF55604. SSF55604. 1 hit.
    TIGRFAMsi TIGR00826. EIIB_glc. 1 hit.
    TIGR00830. PTBA. 1 hit.
    TIGR02002. PTS-II-BC-glcB. 1 hit.
    PROSITEi PS51093. PTS_EIIA_TYPE_1. 1 hit.
    PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit.
    PS51098. PTS_EIIB_TYPE_1. 1 hit.
    PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
    PS51103. PTS_EIIC_TYPE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: N315.
    2. "Shotgun proteomic analysis of total and membrane protein extracts of S. aureus strain N315."
      Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F.
      Submitted (OCT-2007) to UniProtKB
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: N315.

    Entry informationi

    Entry nameiPTU3C_STAAN
    AccessioniPrimary (citable) accession number: Q7A3G4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 14, 2008
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3