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Protein

PTS system glucoside-specific EIICBA component

Gene

glcB

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in alpha- and beta-glucoside transport (By similarity).By similarity

Catalytic activityi

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.PROSITE-ProRule annotation
Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei460Phosphocysteine intermediate; for EIIB activityPROSITE-ProRule annotation1
Active sitei612Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system glucoside-specific EIICBA component
Including the following 3 domains:
Glucoside permease IIC component
Alternative name(s):
PTS system glucoside-specific EIIC component
Glucoside-specific phosphotransferase enzyme IIB component (EC:2.7.1.-)
Alternative name(s):
PTS system glucoside-specific EIIB component
Glucoside-specific phosphotransferase enzyme IIA component
Alternative name(s):
PTS system glucoside-specific EIIA component
Gene namesi
Name:glcB
Ordered Locus Names:SA2326
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000751 Componenti: Chromosome

Subcellular locationi

  • Cell membrane PROSITE-ProRule annotation; Multi-pass membrane protein PROSITE-ProRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei12 – 32HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei81 – 101HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei137 – 157HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei182 – 202HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei223 – 243HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei284 – 304HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei315 – 335HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei340 – 360HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei364 – 384HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei395 – 415HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003514141 – 688PTS system glucoside-specific EIICBA componentAdd BLAST688

Structurei

3D structure databases

ProteinModelPortaliQ7A3G4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 427PTS EIIC type-1PROSITE-ProRule annotationAdd BLAST425
Domaini438 – 519PTS EIIB type-1PROSITE-ProRule annotationAdd BLAST82
Domaini560 – 664PTS EIIA type-1PROSITE-ProRule annotationAdd BLAST105

Domaini

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.

Sequence similaritiesi

Contains 1 PTS EIIA type-1 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIB type-1 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIC type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000250993.
KOiK20116.
K20117.
K20118.
OMAiIFGYIER.

Family and domain databases

CDDicd00212. PTS_IIB_glc. 1 hit.
Gene3Di3.30.1360.60. 1 hit.
InterProiIPR011055. Dup_hybrid_motif.
IPR018113. PTrfase_EIIB_Cys.
IPR001127. PTS_EIIA_1_perm.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
IPR011299. PTS_IIBC_glc.
[Graphical view]
PfamiPF00358. PTS_EIIA_1. 1 hit.
PF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMiSSF51261. SSF51261. 1 hit.
SSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
TIGR00830. PTBA. 1 hit.
TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITEiPS51093. PTS_EIIA_TYPE_1. 1 hit.
PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit.
PS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7A3G4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKKLFGQLQ RIGKALMLPV AILPAAGILL AFGNAMHNEQ LVEIAPWLKN
60 70 80 90 100
DIIVMISSVM EAAGQVVFDN LPLLFAVGTA LGLAGGDGVA ALAALVGYLI
110 120 130 140 150
MNATMGKVLH ITIDDIFSYA KGAKELSQAA KEPAHALVLG IPTLQTGVFG
160 170 180 190 200
GIIMGALAAW CYNKFYNITL PPFLGFFAGK RFVPIVTSVV AIATGVLLSF
210 220 230 240 250
AWPPIQDGLN SLSNFLLNKN LTLTTFIFGI IERSLIPFGL HHIFYSPFWF
260 270 280 290 300
EFGSYTNHAG ELVRGDQRIW MAQLKDGVPF TAGAFTTGKY PFMMFGLPAA
310 320 330 340 350
AFAIYKNARP ERKKVVGGLM LSAGLTAFLT GITEPLEFSF LFVAPVLYGI
360 370 380 390 400
HVLLAGTSFL VMHLLGVKIG MTFSGGFIDY ILYGLLNWDR SHALLVIPVG
410 420 430 440 450
IVYAIVYYFL FDFAIRKFKL KTPGREDEET EIRNSSVAKL PFDVLDAMGG
460 470 480 490 500
KENIKHLDAC ITRLRVEVVD KSKVDVAGIK ALGASGVLEV GNNMQAIFGP
510 520 530 540 550
KSDQIKHDMA KIMSGEITKP SETTVTEEMS DEPVHVEALG TTDIYAPGVG
560 570 580 590 600
QIIPLSEVPD QVFAGKMMGD GIGFIPEKGE IVAPFDGTVK TIFPTKHAIG
610 620 630 640 650
LESESGVEVL IHIGIDTVKL NGEGFESLIN VDEKVTQAQP LMKVNLAYLK
660 670 680
AHAPSIVTPM IITNLENKEL VIEDVQDADP GKLIMTVK
Length:688
Mass (Da):74,430
Last modified:July 5, 2004 - v1
Checksum:i11D5C824835AFD21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB43629.1.
PIRiC90058.
RefSeqiWP_000473678.1. NC_002745.2.

Genome annotation databases

EnsemblBacteriaiBAB43629; BAB43629; BAB43629.
KEGGisau:SA2326.
PATRICi19577210. VBIStaAur116463_2523.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB43629.1.
PIRiC90058.
RefSeqiWP_000473678.1. NC_002745.2.

3D structure databases

ProteinModelPortaliQ7A3G4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB43629; BAB43629; BAB43629.
KEGGisau:SA2326.
PATRICi19577210. VBIStaAur116463_2523.

Phylogenomic databases

HOGENOMiHOG000250993.
KOiK20116.
K20117.
K20118.
OMAiIFGYIER.

Family and domain databases

CDDicd00212. PTS_IIB_glc. 1 hit.
Gene3Di3.30.1360.60. 1 hit.
InterProiIPR011055. Dup_hybrid_motif.
IPR018113. PTrfase_EIIB_Cys.
IPR001127. PTS_EIIA_1_perm.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
IPR011299. PTS_IIBC_glc.
[Graphical view]
PfamiPF00358. PTS_EIIA_1. 1 hit.
PF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMiSSF51261. SSF51261. 1 hit.
SSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
TIGR00830. PTBA. 1 hit.
TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITEiPS51093. PTS_EIIA_TYPE_1. 1 hit.
PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit.
PS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTU3C_STAAN
AccessioniPrimary (citable) accession number: Q7A3G4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.