pyk

Functionⁱ

Catalytic activityⁱ

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactorⁱ

Protein has several cofactor binding sites:

Mg²⁺By similarity
K⁺By similarity

Pathwayⁱ: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:

This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	32	SubstrateBy similarity	1
Metal bindingⁱ	34	PotassiumBy similarity	1
Metal bindingⁱ	36	PotassiumBy similarity	1
Metal bindingⁱ	66	PotassiumBy similarity	1
Metal bindingⁱ	67	Potassium; via carbonyl oxygenBy similarity	1
Siteⁱ	219	Transition state stabilizerBy similarity	1
Metal bindingⁱ	221	MagnesiumBy similarity	1
Binding siteⁱ	244	Substrate; via amide nitrogenBy similarity	1
Metal bindingⁱ	245	MagnesiumBy similarity	1
Binding siteⁱ	245	Substrate; via amide nitrogenBy similarity	1
Binding siteⁱ	277	SubstrateBy similarity	1

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
kinase activity Source: UniProtKB-KW
magnesium ion binding Source: InterPro
potassium ion binding Source: InterPro
pyruvate kinase activity Source: UniProtKB-EC

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Transferase
Biological process	Glycolysis
Ligand	ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayⁱ	UPA00109; UER00188.

UniPathwayⁱ

UPA00109; UER00188.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Pyruvate kinase (EC:2.7.1.40) Short name: PK
Gene namesⁱ	Name:pyk Ordered Locus Names:MW1641
Organismⁱ	Staphylococcus aureus (strain MW2)
Taxonomic identifierⁱ	196620 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Terrabacteria group › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus
Proteomesⁱ	UP000000418 Componentⁱ: Chromosome

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000294133	1 – 585	Pyruvate kinaseAdd BLAST		585

Proteomic databases

PRIDEⁱ	Q7A0N4.

PRIDEⁱ

Q7A0N4.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q7A0N4.
SMRⁱ	Q7A0N4.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the pyruvate kinase family.Curated

In the C-terminal section; belongs to the PEP-utilizing enzyme family.Curated

Phylogenomic databases

HOGENOMⁱ	HOG000021559.
KEGG Orthology (KO) More...KOⁱ	K00873.
OMAⁱ	KHEAIEQ.

Family and domain databases

Gene3Dⁱ	2.40.33.10. 1 hit. 3.40.1380.20. 1 hit.
InterProⁱ	View protein in InterPro IPR008279. PEP-util_enz_mobile_dom. IPR036637. Phosphohistidine_dom_sf. IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase-like_dom. IPR011037. Pyrv_Knase-like_insert_dom_sf. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C. IPR036918. Pyrv_Knase_C_sf. IPR015806. Pyrv_Knase_insert_dom_sf.
PANTHERⁱ	PTHR11817. PTHR11817. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00391. PEP-utilizers. 1 hit. PF00224. PK. 1 hit. PF02887. PK_C. 1 hit.
PRINTSⁱ	PR01050. PYRUVTKNASE.
SUPFAMⁱ	SSF50800. SSF50800. 1 hit. SSF51621. SSF51621. 2 hits. SSF52009. SSF52009. 1 hit. SSF52935. SSF52935. 1 hit.
TIGRFAMsⁱ	TIGR01064. pyruv_kin. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q7A0N4-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MRKTKIVCTI GPASESEEMI EKLINAGMNV ARLNFSHGSH EEHKGRIDTI 
        60         70         80         90        100
RKVAKRLDKI VAILLDTKGP EIRTHNMKDG IIELERGNEV IVSMNEVEGT 
       110        120        130        140        150
PEKFSVTYEN LINDVQVGSY ILLDDGLIEL QVKDIDHAKK EVKCDILNSG 
       160        170        180        190        200
ELKNKKGVNL PGVRVSLPGI TEKDAEDIRF GIKENVDFIA ASFVRRPSDV 
       210        220        230        240        250
LEIREILEEQ KANISVFPKI ENQEGIDNIA EILEVSDGLM VARGDMGVEI 
       260        270        280        290        300
PPEKVPMVQK DLIRQCNKLG KPVITATQML DSMQRNPRAT RAEASDVANA 
       310        320        330        340        350
IYDGTDAVML SGETAAGLYP EEAVKTMRNI AVSAEAAQDY KKLLSDRTKL 
       360        370        380        390        400
VETSLVNAIG ISVAHTALNL NVKAIVAATE SGSTARTISK YRPHSDIIAV 
       410        420        430        440        450
TPSEETARQC SIVWGVQPVV KKGRKSTDAL LNNAVATAVE TGRVSNGDLI 
       460        470        480        490        500
IITAGVPTGE TGTTNMMKIH LVGDEIANGQ GIGRGSVVGT TLVAETVKDL 
       510        520        530        540        550
EGKDLSDKVI VTNSIDETFV PYVEKALGLI TEENGITSPS AIVGLEKGIP 
       560        570        580 
TVVGVEKAVK NISNNMLVTI DAAQGKIFEG YANVL

Length:585

Mass (Da):63,102

Last modified:July 5, 2004 - v1

Checksum:ⁱ4AB45FDE55BF577D

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BA000033 Genomic DNA. Translation: BAB95506.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_001232648.1. NC_003923.1.

Genome annotation databases

EnsemblBacteriaⁱ	BAB95506; BAB95506; BAB95506.
KEGGⁱ	sam:MW1641.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q7A0N4	Q2FG40 Q2FXM9 Q5HF76 Q99TG5 Q7A559 Q6G8M9 W8UX29 A0A2K4E0Q7 T1YAS0 A0A0E1AHF6 +24	Staphylococcus aureus (strain USA300) Staphylococcus aureus (strain NCTC 8325) Staphylococcus aureus (strain COL) Staphylococcus aureus (strain Mu50 / ATCC 700699) Staphylococcus aureus (strain N315) Staphylococcus aureus (strain MSSA476) Staphylococcus aureus Staphylococcus aureus subsp. aureus Staphylococcus aureus subsp. aureus CN1 Staphylococcus aureus subsp. aureus Z172 And more	585	UniRef100_Q2FG40	Cluster: Pyruvate kinase	35

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q7A0N4	Q6GG09 A0A0U1MPJ9 A0A0E1XF30 UPI00022F8405 A0A2I8KML8 UPI00059FFCB8 UPI0005C550DF UPI0005B3220E UPI00059FDEDD UPI0003168CC8 +187	Staphylococcus aureus (strain MRSA252) Staphylococcus aureus Staphylococcus aureus subsp. aureus MN8 Staphylococcus Staphylococcus aureus (strain USA300) Staphylococcus aureus (strain NCTC 8325) Staphylococcus aureus (strain COL) Staphylococcus aureus (strain Mu50 / ATCC 700699) Staphylococcus aureus (strain N315) Staphylococcus aureus (strain MSSA476) And more	585	UniRef90_Q6GG09	Cluster: Pyruvate kinase	198

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q7A0N4	Q6GG09 A0A0U1MPJ9 A0A0E1XF30 UPI00022F8405 UPI000CD2B17E UPI0004483BEE UPI000451A161 UPI000503BDF3 UPI00085BE411 UPI0008A573D1 +484	Staphylococcus aureus (strain MRSA252) Staphylococcus aureus Staphylococcus aureus subsp. aureus MN8 Staphylococcus argenteus Staphylococcus sp. HMSC055A09 Staphylococcus capitis Staphylococcus warneri Staphylococcus schweitzeri Staphylococcus sp. HMSC058E01 Staphylococcus simiae CCM 7213 And more	585	UniRef50_Q6GG09	Cluster: Pyruvate kinase	495

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BA000033 Genomic DNA. Translation: BAB95506.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_001232648.1. NC_003923.1.

3D structure databases

ProteinModelPortalⁱ	Q7A0N4.
SMRⁱ	Q7A0N4.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Proteomic databases

PRIDEⁱ	Q7A0N4.

PRIDEⁱ

Q7A0N4.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	BAB95506; BAB95506; BAB95506.
KEGGⁱ	sam:MW1641.

Phylogenomic databases

HOGENOMⁱ	HOG000021559.
KEGG Orthology (KO) More...KOⁱ	K00873.
OMAⁱ	KHEAIEQ.

Enzyme and pathway databases

UniPathwayⁱ	UPA00109; UER00188.

UniPathwayⁱ

UPA00109; UER00188.

Family and domain databases

Gene3Dⁱ	2.40.33.10. 1 hit. 3.40.1380.20. 1 hit.
InterProⁱ	View protein in InterPro IPR008279. PEP-util_enz_mobile_dom. IPR036637. Phosphohistidine_dom_sf. IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase-like_dom. IPR011037. Pyrv_Knase-like_insert_dom_sf. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C. IPR036918. Pyrv_Knase_C_sf. IPR015806. Pyrv_Knase_insert_dom_sf.
PANTHERⁱ	PTHR11817. PTHR11817. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00391. PEP-utilizers. 1 hit. PF00224. PK. 1 hit. PF02887. PK_C. 1 hit.
PRINTSⁱ	PR01050. PYRUVTKNASE.
SUPFAMⁱ	SSF50800. SSF50800. 1 hit. SSF51621. SSF51621. 2 hits. SSF52009. SSF52009. 1 hit. SSF52935. SSF52935. 1 hit.
TIGRFAMsⁱ	TIGR01064. pyruv_kin. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	KPYK_STAAW
Accessionⁱ	Q7A0N4Primary (citable) accession number: Q7A0N4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
	Last sequence update:	July 5, 2004
	Last modified:	February 28, 2018
	This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome

UniProtKB - Q7A0N4 (KPYK_STAAW)

Your basket is currently empty. ⁱ

Pyruvate kinase

Select a section on the left to see content.

Functionⁱ

Catalytic activityⁱ

Cofactorⁱ

Pathwayⁱ: glycolysis

Sites

GO - Molecular functionⁱ

Keywordsⁱ

Enzyme and pathway databases

Names & Taxonomyⁱ

PTM / Processingⁱ

Molecule processing

Proteomic databases

Structureⁱ

3D structure databases

Family & Domainsⁱ

Sequence similaritiesⁱ

Phylogenomic databases

Family and domain databases

Sequenceⁱ

Sequence databases

Genome annotation databases

Similar proteinsⁱ

Cross-referencesⁱ

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q7A0N4 (KPYK_STAAW)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Pyruvate kinase

pyk

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glycolysis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Your basket is currently empty. ⁱ

Functionⁱ

Pathwayⁱ: glycolysis

GO - Molecular functionⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ