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Protein

Urease subunit alpha

Gene

ureC

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit gamma (ureA), Urease subunit alpha (ureC), Urease subunit beta (ureB)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi137 – 1371Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi139 – 1391Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi220 – 2201Nickel 1; via carbamate groupUniRule annotation
Metal bindingi220 – 2201Nickel 2; via carbamate groupUniRule annotation
Binding sitei222 – 2221SubstrateUniRule annotation
Metal bindingi249 – 2491Nickel 2; via pros nitrogenUniRule annotation
Metal bindingi275 – 2751Nickel 2; via tele nitrogenUniRule annotation
Active sitei323 – 3231Proton donorUniRule annotation
Metal bindingi363 – 3631Nickel 1UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Protein family/group databases

MEROPSiM38.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureCUniRule annotation
Ordered Locus Names:Cgl0086, cg0115
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cells are urease negative and are no longer able to transport urea.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 570570Urease subunit alphaPRO_0000234152Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei220 – 2201N6-carboxylysineUniRule annotation

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Expressioni

Inductioni

By urea and nitrogen starvation.2 Publications

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi196627.cg0115.

Structurei

3D structure databases

ProteinModelPortaliQ79VJ3.
SMRiQ79VJ3. Positions 5-570.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini132 – 570439UreaseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the urease family.UniRule annotation
Contains 1 urease domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
KOiK01428.
OMAiFDSHIHF.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q79VJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFEISRKQY TDLYGPTVGD SVRLADTELF LCVEKDYAAI GEEVAFGGGK
60 70 80 90 100
VIRDGMGQNG TLVRDVDIPD TVITNVIVLD YTGVYKADVA LRDGKIFRIG
110 120 130 140 150
KAGNPNVMEN VDIVIGVATD IIAGEGKILT AGGIDTHVHF LGTDQVNTAL
160 170 180 190 200
ASGITTMIGG GTGPSQASMA TTVTPGQWNT YNMLSAFEGM PMNFGILGKG
210 220 230 240 250
HGSSKSPLAE QVRAGAIGLK IHEDWGATPS SINTALEVAD DMDIQVALHS
260 270 280 290 300
DTLNEAGFVE DTIEAIAGRV IHTFHTEGAG GGHAPDLIRV AALPNVLPAS
310 320 330 340 350
TNPTLPYTRN TVEEHLDMVM VAHHLNPDIP EDVAFADSRI RAETIAAEDV
360 370 380 390 400
LHDMGIFSIT SSDSQAMGRV GETITRTWQV ADHMKRTRGS LTGDAPYNDN
410 420 430 440 450
NRLRRFIAKY TINPAIAHGV DYVVGSVEEG KFADLVLWDP KFFGVKPDLV
460 470 480 490 500
IKGGLMVNSL MGDSNGSIPT PQPRTLRNTW GAFGQAVSRS SITFLSQDAI
510 520 530 540 550
DANVPDLLNL RKQIRGVRGV RNLTKRDMKL NAEMPDIRVD PETYQVFVNG
560 570
ELITSKPAET VPMARRYFLF
Length:570
Mass (Da):61,452
Last modified:July 5, 2004 - v1
Checksum:i2C5F5D9F7F5FBB2E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401I → V in BAA88554 (PubMed:11328647).Curated
Sequence conflicti133 – 1342GI → AL in BAA88554 (PubMed:11328647).Curated
Sequence conflicti206 – 2061S → A in BAA88554 (PubMed:11328647).Curated
Sequence conflicti259 – 2635VEDTI → CGRH in BAA88554 (PubMed:11328647).Curated
Sequence conflicti269 – 2691R → G in BAA88554 (PubMed:11328647).Curated
Sequence conflicti275 – 2751H → N in BAA88554 (PubMed:11328647).Curated
Sequence conflicti286 – 2861D → E in BAA88554 (PubMed:11328647).Curated
Sequence conflicti289 – 2891R → G in BAA88554 (PubMed:11328647).Curated
Sequence conflicti292 – 2921A → G in BAA88554 (PubMed:11328647).Curated
Sequence conflicti303 – 3075PTLPY → QRFRN in BAA88554 (PubMed:11328647).Curated
Sequence conflicti436 – 4361V → L in BAA88554 (PubMed:11328647).Curated
Sequence conflicti516 – 5161G → D in BAA88554 (PubMed:11328647).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB029154 Genomic DNA. Translation: BAA88554.1.
AJ251883 Genomic DNA. Translation: CAB81937.1.
BA000036 Genomic DNA. Translation: BAB97479.1.
BX927148 Genomic DNA. Translation: CAF18654.1.
RefSeqiNP_599338.1. NC_003450.3.
WP_011013378.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB97479; BAB97479; BAB97479.
CAF18654; CAF18654; cg0115.
GeneIDi1021080.
KEGGicgb:cg0115.
cgl:NCgl0085.
PATRICi21492216. VBICorGlu203724_0087.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB029154 Genomic DNA. Translation: BAA88554.1.
AJ251883 Genomic DNA. Translation: CAB81937.1.
BA000036 Genomic DNA. Translation: BAB97479.1.
BX927148 Genomic DNA. Translation: CAF18654.1.
RefSeqiNP_599338.1. NC_003450.3.
WP_011013378.1. NC_006958.1.

3D structure databases

ProteinModelPortaliQ79VJ3.
SMRiQ79VJ3. Positions 5-570.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg0115.

Protein family/group databases

MEROPSiM38.982.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB97479; BAB97479; BAB97479.
CAF18654; CAF18654; cg0115.
GeneIDi1021080.
KEGGicgb:cg0115.
cgl:NCgl0085.
PATRICi21492216. VBICorGlu203724_0087.

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
KOiK01428.
OMAiFDSHIHF.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiURE1_CORGL
AccessioniPrimary (citable) accession number: Q79VJ3
Secondary accession number(s): Q9L420, Q9RHM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.