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Protein

CRP-like cAMP-activated global transcriptional regulator

Gene

glxR

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Global transcriptional regulator that complexes with cAMP and binds to specific DNA promoter sites, causing DNA-bending, to regulate transcription. cAMP improves binding to specific DNA sequences, probably by altering protein conformation. Involved in the regulation of gntP and gntK genes, which are involved in gluconate metabolism (PubMed:16385030). May form dimers which bind to the aceB promoter region in the presence of cAMP and repress the glyoxylate bypass genes (PubMed:15150232). It could be a positive regulator of rpf2 gene expression during growth on acetate as the sole carbon source, however because the cytosolic cAMP level is elevated in the presence of glucose and low upon growth on acetate, it is conceivable that it is unable to function as an activator under acetate conditions (PubMed:18355281).3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi67 – 737cAMP 1By similarity
Nucleotide bindingi82 – 854cAMP 11 Publication
Nucleotide bindingi92 – 932cAMP 11 Publication
Nucleotide bindingi137 – 1382cAMP 11 Publication
Nucleotide bindingi145 – 1462cAMP 2By similarity
DNA bindingi180 – 19920H-T-H motifPROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi181 – 19111cAMP 2By similarityAdd
BLAST

GO - Molecular functioni

  • cAMP binding Source: UniProtKB
  • DNA binding Source: UniProtKB
  • kinase activity Source: UniProtKB-KW

GO - Biological processi

  • regulation of glyoxylate cycle Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Repressor, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

cAMP, cAMP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCGLU1204414-WGS:GSME-286-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CRP-like cAMP-activated global transcriptional regulatorBy similarity
Alternative name(s):
cAMP receptor proteinBy similarity
Short name:
CRPBy similarity
cAMP regulatory proteinBy similarity
Gene namesi
Name:glxR1 Publication
Ordered Locus Names:cg0350, Cgl0291
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227CRP-like cAMP-activated global transcriptional regulatorPRO_0000433043Add
BLAST

Expressioni

Inductioni

Seems to repress its own expression.1 Publication

Gene expression databases

CollecTFiEXPREG_00000f90.

Interactioni

Subunit structurei

Homodimer.3 Publications

Protein-protein interaction databases

STRINGi196627.cg0350.

Structurei

Secondary structure

1
227
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 96Combined sources
Helixi12 – 143Combined sources
Helixi19 – 279Combined sources
Beta strandi29 – 346Combined sources
Beta strandi39 – 413Combined sources
Beta strandi50 – 567Combined sources
Beta strandi58 – 636Combined sources
Beta strandi65 – 673Combined sources
Beta strandi69 – 757Combined sources
Beta strandi80 – 823Combined sources
Helixi84 – 874Combined sources
Beta strandi93 – 1008Combined sources
Beta strandi102 – 1076Combined sources
Helixi109 – 11810Combined sources
Helixi120 – 14627Combined sources
Helixi149 – 16416Combined sources
Beta strandi166 – 1683Combined sources
Beta strandi171 – 1744Combined sources
Helixi180 – 1878Combined sources
Helixi191 – 20313Combined sources
Beta strandi206 – 2094Combined sources
Beta strandi214 – 2163Combined sources
Helixi219 – 2257Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3R6SX-ray2.38A/B/C/D/E/F1-227[»]
4BYYX-ray2.48A/B1-227[»]
ProteinModelPortaliQ79VI7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini147 – 22074HTH crp-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105IJJ. Bacteria.
COG0664. LUCA.
HOGENOMiHOG000250566.
KOiK01420.
OMAiARIMAMC.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP.
IPR014710. RmlC-like_jellyroll.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF13545. HTH_Crp_2. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q79VI7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGVQEILSR AGIFQGVDPT AVNNLIQDME TVRFPRGATI FDEGEPGDRL
60 70 80 90 100
YIITSGKVKL ARHAPDGREN LLTIMGPSDM FGELSIFDPG PRTSSAVCVT
110 120 130 140 150
EVHAATMNSD MLRNWVADHP AIAEQLLRVL ARRLRRTNAS LADLIFTDVP
160 170 180 190 200
GRVAKTLLQL ANRFGTQEAG ALRVNHDLTQ EEIAQLVGAS RETVNKALAT
210 220
FAHRGWIRLE GKSVLIVDTE HLARRAR
Length:227
Mass (Da):24,988
Last modified:July 5, 2004 - v1
Checksum:i80D89C13246FD71D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB97684.1.
BX927148 Genomic DNA. Translation: CAF18861.1.
RefSeqiNP_599543.1. NC_003450.3.
WP_003855810.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB97684; BAB97684; BAB97684.
CAF18861; CAF18861; cg0350.
GeneIDi1021325.
KEGGicgb:cg0350.
cgl:NCgl0286.
PATRICi21492646. VBICorGlu203724_0295.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB97684.1.
BX927148 Genomic DNA. Translation: CAF18861.1.
RefSeqiNP_599543.1. NC_003450.3.
WP_003855810.1. NC_006958.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3R6SX-ray2.38A/B/C/D/E/F1-227[»]
4BYYX-ray2.48A/B1-227[»]
ProteinModelPortaliQ79VI7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg0350.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB97684; BAB97684; BAB97684.
CAF18861; CAF18861; cg0350.
GeneIDi1021325.
KEGGicgb:cg0350.
cgl:NCgl0286.
PATRICi21492646. VBICorGlu203724_0295.

Phylogenomic databases

eggNOGiENOG4105IJJ. Bacteria.
COG0664. LUCA.
HOGENOMiHOG000250566.
KOiK01420.
OMAiARIMAMC.

Enzyme and pathway databases

BioCyciCGLU1204414-WGS:GSME-286-MONOMER.

Gene expression databases

CollecTFiEXPREG_00000f90.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP.
IPR014710. RmlC-like_jellyroll.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF13545. HTH_Crp_2. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCRPL_CORGL
AccessioniPrimary (citable) accession number: Q79VI7
Secondary accession number(s): Q93PX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2015
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.