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Protein

CRP-like cAMP-activated global transcriptional regulator

Gene

glxR

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Global transcriptional regulator that complexes with cAMP and binds to specific DNA promoter sites, causing DNA-bending, to regulate transcription. cAMP improves binding to specific DNA sequences, probably by altering protein conformation. Involved in the regulation of gntP and gntK genes, which are involved in gluconate metabolism (PubMed:16385030). May form dimers which bind to the aceB promoter region in the presence of cAMP and repress the glyoxylate bypass genes (PubMed:15150232). It could be a positive regulator of rpf2 gene expression during growth on acetate as the sole carbon source, however because the cytosolic cAMP level is elevated in the presence of glucose and low upon growth on acetate, it is conceivable that it is unable to function as an activator under acetate conditions (PubMed:18355281).3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi67 – 73cAMP 1By similarity7
Nucleotide bindingi82 – 85cAMP 11 Publication4
Nucleotide bindingi92 – 93cAMP 11 Publication2
Nucleotide bindingi137 – 138cAMP 11 Publication2
Nucleotide bindingi145 – 146cAMP 2By similarity2
DNA bindingi180 – 199H-T-H motifPROSITE-ProRule annotationAdd BLAST20
Nucleotide bindingi181 – 191cAMP 2By similarityAdd BLAST11

GO - Molecular functioni

  • cAMP binding Source: UniProtKB
  • DNA binding Source: UniProtKB
  • kinase activity Source: UniProtKB-KW

GO - Biological processi

  • regulation of glyoxylate cycle Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Kinase, Repressor, Transferase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

cAMP, cAMP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCGLU1204414:G134W-286-MONOMER.
CORYNE:G18NG-9847-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CRP-like cAMP-activated global transcriptional regulatorBy similarity
Alternative name(s):
cAMP receptor proteinBy similarity
Short name:
CRPBy similarity
cAMP regulatory proteinBy similarity
Gene namesi
Name:glxR1 Publication
Ordered Locus Names:cg0350, Cgl0291
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004330431 – 227CRP-like cAMP-activated global transcriptional regulatorAdd BLAST227

Expressioni

Inductioni

Seems to repress its own expression.1 Publication

Gene expression databases

CollecTFiEXPREG_00000f90.

Interactioni

Subunit structurei

Homodimer.3 Publications

Protein-protein interaction databases

STRINGi196627.cg0350.

Structurei

Secondary structure

1227
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 9Combined sources6
Helixi12 – 14Combined sources3
Helixi19 – 27Combined sources9
Beta strandi29 – 34Combined sources6
Beta strandi39 – 41Combined sources3
Beta strandi50 – 56Combined sources7
Beta strandi58 – 63Combined sources6
Beta strandi65 – 67Combined sources3
Beta strandi69 – 75Combined sources7
Beta strandi80 – 82Combined sources3
Helixi84 – 87Combined sources4
Beta strandi93 – 100Combined sources8
Beta strandi102 – 107Combined sources6
Helixi109 – 118Combined sources10
Helixi120 – 146Combined sources27
Helixi149 – 164Combined sources16
Beta strandi166 – 168Combined sources3
Beta strandi171 – 174Combined sources4
Helixi180 – 187Combined sources8
Helixi191 – 203Combined sources13
Beta strandi206 – 209Combined sources4
Beta strandi214 – 216Combined sources3
Helixi219 – 225Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3R6SX-ray2.38A/B/C/D/E/F1-227[»]
4BYYX-ray2.48A/B1-227[»]
ProteinModelPortaliQ79VI7.
SMRiQ79VI7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini147 – 220HTH crp-typePROSITE-ProRule annotationAdd BLAST74

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105IJJ. Bacteria.
COG0664. LUCA.
HOGENOMiHOG000250566.
KOiK01420.
OMAiARIMAMC.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP.
IPR014710. RmlC-like_jellyroll.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF13545. HTH_Crp_2. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q79VI7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGVQEILSR AGIFQGVDPT AVNNLIQDME TVRFPRGATI FDEGEPGDRL
60 70 80 90 100
YIITSGKVKL ARHAPDGREN LLTIMGPSDM FGELSIFDPG PRTSSAVCVT
110 120 130 140 150
EVHAATMNSD MLRNWVADHP AIAEQLLRVL ARRLRRTNAS LADLIFTDVP
160 170 180 190 200
GRVAKTLLQL ANRFGTQEAG ALRVNHDLTQ EEIAQLVGAS RETVNKALAT
210 220
FAHRGWIRLE GKSVLIVDTE HLARRAR
Length:227
Mass (Da):24,988
Last modified:July 5, 2004 - v1
Checksum:i80D89C13246FD71D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB97684.1.
BX927148 Genomic DNA. Translation: CAF18861.1.
RefSeqiNP_599543.1. NC_003450.3.
WP_003855810.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB97684; BAB97684; BAB97684.
CAF18861; CAF18861; cg0350.
GeneIDi1021325.
KEGGicgb:cg0350.
cgl:NCgl0286.
PATRICi21492646. VBICorGlu203724_0295.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB97684.1.
BX927148 Genomic DNA. Translation: CAF18861.1.
RefSeqiNP_599543.1. NC_003450.3.
WP_003855810.1. NC_006958.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3R6SX-ray2.38A/B/C/D/E/F1-227[»]
4BYYX-ray2.48A/B1-227[»]
ProteinModelPortaliQ79VI7.
SMRiQ79VI7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg0350.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB97684; BAB97684; BAB97684.
CAF18861; CAF18861; cg0350.
GeneIDi1021325.
KEGGicgb:cg0350.
cgl:NCgl0286.
PATRICi21492646. VBICorGlu203724_0295.

Phylogenomic databases

eggNOGiENOG4105IJJ. Bacteria.
COG0664. LUCA.
HOGENOMiHOG000250566.
KOiK01420.
OMAiARIMAMC.

Enzyme and pathway databases

BioCyciCGLU1204414:G134W-286-MONOMER.
CORYNE:G18NG-9847-MONOMER.

Gene expression databases

CollecTFiEXPREG_00000f90.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP.
IPR014710. RmlC-like_jellyroll.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF13545. HTH_Crp_2. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCRPL_CORGL
AccessioniPrimary (citable) accession number: Q79VI7
Secondary accession number(s): Q93PX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2015
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.