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Protein

Circadian clock protein kinase KaiC

Gene

kaiC

Organism
Thermosynechococcus elongatus (strain BP-1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. Binds to DNA. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

The interaction with KaiA enhances its phosphorylation status, while the interaction with KaiB decreases it. A KaiA dimer is sufficient to enhance KaiC phosphorylation (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi295 – 2951MagnesiumBy similarity
Metal bindingi318 – 3181MagnesiumBy similarity
Metal bindingi319 – 3191MagnesiumBy similarity
Metal bindingi378 – 3781MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi47 – 548ATPBy similarity
Nucleotide bindingi288 – 2958ATPBy similarity

GO - Molecular functioni

GO - Biological processi

  • circadian rhythm Source: UniProtKB
  • protein autophosphorylation Source: UniProtKB
  • regulation of circadian rhythm Source: InterPro
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Repressor, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Biological rhythms, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Circadian clock protein kinase KaiC (EC:2.7.11.1)
Gene namesi
Name:kaiC
Ordered Locus Names:tlr0483
OrganismiThermosynechococcus elongatus (strain BP-1)
Taxonomic identifieri197221 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesThermosynechococcus
Proteomesi
  • UP000000440 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 518518Circadian clock protein kinase KaiCPRO_0000217779Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei432 – 4321Phosphothreonine; by autocatalysisBy similarity

Post-translational modificationi

Phosphorylated on serine/threonine residues by autocatalysis. Both phosphorylated and unphosphorylated forms exist. Can probably autophosphorylate and autodephosphorylate. Phosphorylated form correlates with clock speed (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homohexamer; hexamerization is dependent on ATP-binding. Core component of the KaiABC complex, at least composed of a KaiC homohexamer, a KaiB dimer and two KaiA dimers. Interacts directly with SasA (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
itself6EBI-701595,EBI-701595
kaiAQ79V6211EBI-701595,EBI-701584
kaiBQ79V615EBI-701595,EBI-7570699

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-29357N.
IntActiQ79V60. 2 interactions.
MINTiMINT-1954047.
STRINGi197221.tlr0483.

Structurei

Secondary structure

1
518
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi29 – 324Combined sources
Beta strandi35 – 384Combined sources
Beta strandi41 – 488Combined sources
Helixi53 – 6816Combined sources
Beta strandi72 – 798Combined sources
Helixi81 – 888Combined sources
Helixi89 – 913Combined sources
Helixi96 – 1005Combined sources
Beta strandi103 – 1086Combined sources
Beta strandi119 – 1224Combined sources
Helixi128 – 13811Combined sources
Beta strandi141 – 1466Combined sources
Helixi149 – 1513Combined sources
Turni152 – 1543Combined sources
Helixi158 – 17417Combined sources
Beta strandi178 – 1847Combined sources
Beta strandi186 – 1905Combined sources
Beta strandi192 – 1965Combined sources
Helixi198 – 2014Combined sources
Beta strandi203 – 2119Combined sources
Beta strandi218 – 2269Combined sources
Beta strandi234 – 24613Combined sources
Turni249 – 2513Combined sources
Helixi268 – 2736Combined sources
Beta strandi276 – 2816Combined sources
Beta strandi283 – 2897Combined sources
Helixi294 – 30714Combined sources
Beta strandi312 – 3198Combined sources
Helixi321 – 3299Combined sources
Turni330 – 3323Combined sources
Helixi335 – 3406Combined sources
Beta strandi343 – 3486Combined sources
Helixi351 – 3533Combined sources
Helixi356 – 37015Combined sources
Beta strandi373 – 3786Combined sources
Turni381 – 3866Combined sources
Helixi389 – 40517Combined sources
Beta strandi409 – 4157Combined sources
Beta strandi435 – 44511Combined sources
Beta strandi448 – 45811Combined sources
Beta strandi468 – 4736Combined sources
Beta strandi476 – 4827Combined sources
Helixi506 – 5083Combined sources
Helixi512 – 5154Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SUYNMR-C/D488-518[»]
1SV1NMR-C/D488-518[»]
4O0MX-ray2.84A/B/C1-518[»]
ProteinModelPortaliQ79V60.
SMRiQ79V60. Positions 19-518.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ79V60.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 260241KaiC 1Add
BLAST
Domaini261 – 493233KaiC 2Add
BLAST

Domaini

The KaiC domains mediate the interaction with KaiA.By similarity

Sequence similaritiesi

Belongs to the KaiC family.Curated
Contains 2 kaiC domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105EHU. Bacteria.
COG0467. LUCA.
HOGENOMiHOG000230416.
KOiK08482.
OMAiICAYPES.
OrthoDBiEOG6ZPSVX.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01836. KaiC.
InterProiIPR013503. Circadian_KaiC_bact.
IPR030665. KaiC.
IPR014774. KaiC-like_dom.
IPR010624. KaiC_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF06745. ATPase. 2 hits.
[Graphical view]
PIRSFiPIRSF039117. KaiC. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR02655. circ_KaiC. 1 hit.
PROSITEiPS51146. KAIC. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q79V60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNLPEHQSS PTEQSSAEVK KIPTMIEGFD DISHGGLPQG RTTLVSGTSG
60 70 80 90 100
TGKTLFAVQF LYNGITIFNE PGIFVTFEES PQDIIKNALS FGWNLQSLID
110 120 130 140 150
QGKLFILDAS PDPDGQEVAG DFDLSALIER IQYAIRKYKA TRVSIDSVTA
160 170 180 190 200
VFQQYDAASV VRREIFRLAF RLKQLGVTTI MTTERVDEYG PVARFGVEEF
210 220 230 240 250
VSDNVVILRN VLEGERRRRT VEILKLRGTT HMKGEYPFTI NNGINIFPLG
260 270 280 290 300
AMRLTQRSSN VRVSSGVKTL DEMCGGGFFK DSIILATGAT GTGKTLLVSK
310 320 330 340 350
FLETGCQQGE RALLFAYEES RAQLSRNASS WGIDFEELER RGLLRIICAY
360 370 380 390 400
PESAGLEDHL QIIKSEIADF KPSRVAIDSL SALARGVSNN AFRQFVIGVT
410 420 430 440 450
GFAKQEEITG FFTNTTDQFM GSNSITESHI STITDTILLL QYVEIRGEMS
460 470 480 490 500
RAINVFKMRG SWHDKGIREY VITEKGAEIR DSFRNFEGII SGTPTRISVD
510
EKTELARIAK GMQDLESE
Length:518
Mass (Da):57,538
Last modified:July 5, 2004 - v1
Checksum:i34E4CDF9DE9C1824
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB071375 Genomic DNA. Translation: BAB85985.1.
BA000039 Genomic DNA. Translation: BAC08035.1.
RefSeqiNP_681273.1. NC_004113.1.
WP_011056334.1. NC_004113.1.

Genome annotation databases

EnsemblBacteriaiBAC08035; BAC08035; BAC08035.
GeneIDi1012389.
KEGGitel:tlr0483.
PATRICi23926210. VBITheElo119873_0508.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB071375 Genomic DNA. Translation: BAB85985.1.
BA000039 Genomic DNA. Translation: BAC08035.1.
RefSeqiNP_681273.1. NC_004113.1.
WP_011056334.1. NC_004113.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SUYNMR-C/D488-518[»]
1SV1NMR-C/D488-518[»]
4O0MX-ray2.84A/B/C1-518[»]
ProteinModelPortaliQ79V60.
SMRiQ79V60. Positions 19-518.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29357N.
IntActiQ79V60. 2 interactions.
MINTiMINT-1954047.
STRINGi197221.tlr0483.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC08035; BAC08035; BAC08035.
GeneIDi1012389.
KEGGitel:tlr0483.
PATRICi23926210. VBITheElo119873_0508.

Phylogenomic databases

eggNOGiENOG4105EHU. Bacteria.
COG0467. LUCA.
HOGENOMiHOG000230416.
KOiK08482.
OMAiICAYPES.
OrthoDBiEOG6ZPSVX.

Miscellaneous databases

EvolutionaryTraceiQ79V60.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01836. KaiC.
InterProiIPR013503. Circadian_KaiC_bact.
IPR030665. KaiC.
IPR014774. KaiC-like_dom.
IPR010624. KaiC_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF06745. ATPase. 2 hits.
[Graphical view]
PIRSFiPIRSF039117. KaiC. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR02655. circ_KaiC. 1 hit.
PROSITEiPS51146. KAIC. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "ATP-induced hexameric ring structure of the cyanobacterial circadian clock protein KaiC."
    Hayashi F., Suzuki H., Iwase R., Uzumaki T., Miyake A., Shen J.-R., Imada K., Furukawa Y., Yonekura K., Namba K., Ishiura M.
    Genes Cells 8:287-296(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], HEXAMERIZATION, ATP-BINDING.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BP-1.
  3. "Structure of the C-terminal domain of the clock protein KaiA in complex with a KaiC-derived peptide: implications for KaiC regulation."
    Vakonakis I., LiWang A.C.
    Proc. Natl. Acad. Sci. U.S.A. 101:10925-10930(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 488-518 IN COMPLEX WITH 180-283 OF KAIA.

Entry informationi

Entry nameiKAIC_THEEB
AccessioniPrimary (citable) accession number: Q79V60
Secondary accession number(s): Q8RR33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.