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Protein

Circadian clock protein kinase KaiC

Gene

kaiC

Organism
Thermosynechococcus elongatus (strain BP-1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. Binds to DNA. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

The interaction with KaiA enhances its phosphorylation status, while the interaction with KaiB decreases it. A KaiA dimer is sufficient to enhance KaiC phosphorylation (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi295MagnesiumBy similarity1
Metal bindingi318MagnesiumBy similarity1
Metal bindingi319MagnesiumBy similarity1
Metal bindingi378MagnesiumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi47 – 54ATPBy similarity8
Nucleotide bindingi288 – 295ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

  • circadian rhythm Source: UniProtKB
  • protein autophosphorylation Source: UniProtKB
  • regulation of circadian rhythm Source: InterPro
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Repressor, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Biological rhythms, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Circadian clock protein kinase KaiC (EC:2.7.11.1)
Gene namesi
Name:kaiC
Ordered Locus Names:tlr0483
OrganismiThermosynechococcus elongatus (strain BP-1)
Taxonomic identifieri197221 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeThermosynechococcus
Proteomesi
  • UP000000440 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002177791 – 518Circadian clock protein kinase KaiCAdd BLAST518

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei432Phosphothreonine; by autocatalysisBy similarity1

Post-translational modificationi

Phosphorylated on serine/threonine residues by autocatalysis. Both phosphorylated and unphosphorylated forms exist. Can probably autophosphorylate and autodephosphorylate. Phosphorylated form correlates with clock speed (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homohexamer; hexamerization is dependent on ATP-binding. Core component of the KaiABC complex, at least composed of a KaiC homohexamer, a KaiB dimer and two KaiA dimers. Interacts directly with SasA (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
itself6EBI-701595,EBI-701595
kaiAQ79V6211EBI-701595,EBI-701584
kaiBQ79V615EBI-701595,EBI-7570699

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-29357N.
IntActiQ79V60. 2 interactors.
MINTiMINT-1954047.
STRINGi197221.tlr0483.

Structurei

Secondary structure

1518
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi29 – 32Combined sources4
Beta strandi35 – 38Combined sources4
Beta strandi41 – 48Combined sources8
Helixi53 – 68Combined sources16
Beta strandi72 – 79Combined sources8
Helixi81 – 88Combined sources8
Helixi89 – 91Combined sources3
Helixi96 – 100Combined sources5
Beta strandi103 – 108Combined sources6
Beta strandi119 – 122Combined sources4
Helixi128 – 138Combined sources11
Beta strandi141 – 146Combined sources6
Helixi149 – 151Combined sources3
Turni152 – 154Combined sources3
Helixi158 – 174Combined sources17
Beta strandi178 – 184Combined sources7
Beta strandi186 – 190Combined sources5
Beta strandi192 – 196Combined sources5
Helixi198 – 201Combined sources4
Beta strandi203 – 211Combined sources9
Beta strandi218 – 226Combined sources9
Beta strandi234 – 246Combined sources13
Turni249 – 251Combined sources3
Helixi268 – 273Combined sources6
Beta strandi276 – 281Combined sources6
Beta strandi283 – 289Combined sources7
Helixi294 – 307Combined sources14
Beta strandi312 – 319Combined sources8
Helixi321 – 329Combined sources9
Turni330 – 332Combined sources3
Helixi335 – 340Combined sources6
Beta strandi343 – 348Combined sources6
Helixi351 – 353Combined sources3
Helixi356 – 370Combined sources15
Beta strandi373 – 378Combined sources6
Turni381 – 386Combined sources6
Helixi389 – 405Combined sources17
Beta strandi409 – 415Combined sources7
Beta strandi435 – 445Combined sources11
Beta strandi448 – 458Combined sources11
Beta strandi468 – 473Combined sources6
Beta strandi476 – 482Combined sources7
Helixi506 – 508Combined sources3
Helixi512 – 515Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SUYNMR-C/D488-518[»]
1SV1NMR-C/D488-518[»]
4O0MX-ray2.84A/B/C1-518[»]
ProteinModelPortaliQ79V60.
SMRiQ79V60.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ79V60.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 260KaiC 1Add BLAST241
Domaini261 – 493KaiC 2Add BLAST233

Domaini

The KaiC domains mediate the interaction with KaiA.By similarity

Sequence similaritiesi

Belongs to the KaiC family.Curated
Contains 2 kaiC domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105EHU. Bacteria.
COG0467. LUCA.
HOGENOMiHOG000230416.
KOiK08482.
OMAiICAYPES.
OrthoDBiPOG091H0SAA.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01836. KaiC. 1 hit.
InterProiIPR013503. Circadian_KaiC_bact.
IPR030665. KaiC.
IPR014774. KaiC-like_dom.
IPR010624. KaiC_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF06745. ATPase. 2 hits.
[Graphical view]
PIRSFiPIRSF039117. KaiC. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR02655. circ_KaiC. 1 hit.
PROSITEiPS51146. KAIC. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q79V60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNLPEHQSS PTEQSSAEVK KIPTMIEGFD DISHGGLPQG RTTLVSGTSG
60 70 80 90 100
TGKTLFAVQF LYNGITIFNE PGIFVTFEES PQDIIKNALS FGWNLQSLID
110 120 130 140 150
QGKLFILDAS PDPDGQEVAG DFDLSALIER IQYAIRKYKA TRVSIDSVTA
160 170 180 190 200
VFQQYDAASV VRREIFRLAF RLKQLGVTTI MTTERVDEYG PVARFGVEEF
210 220 230 240 250
VSDNVVILRN VLEGERRRRT VEILKLRGTT HMKGEYPFTI NNGINIFPLG
260 270 280 290 300
AMRLTQRSSN VRVSSGVKTL DEMCGGGFFK DSIILATGAT GTGKTLLVSK
310 320 330 340 350
FLETGCQQGE RALLFAYEES RAQLSRNASS WGIDFEELER RGLLRIICAY
360 370 380 390 400
PESAGLEDHL QIIKSEIADF KPSRVAIDSL SALARGVSNN AFRQFVIGVT
410 420 430 440 450
GFAKQEEITG FFTNTTDQFM GSNSITESHI STITDTILLL QYVEIRGEMS
460 470 480 490 500
RAINVFKMRG SWHDKGIREY VITEKGAEIR DSFRNFEGII SGTPTRISVD
510
EKTELARIAK GMQDLESE
Length:518
Mass (Da):57,538
Last modified:July 5, 2004 - v1
Checksum:i34E4CDF9DE9C1824
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB071375 Genomic DNA. Translation: BAB85985.1.
BA000039 Genomic DNA. Translation: BAC08035.1.
RefSeqiNP_681273.1. NC_004113.1.
WP_011056334.1. NC_004113.1.

Genome annotation databases

EnsemblBacteriaiBAC08035; BAC08035; BAC08035.
GeneIDi1012389.
KEGGitel:tlr0483.
PATRICi23926210. VBITheElo119873_0508.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB071375 Genomic DNA. Translation: BAB85985.1.
BA000039 Genomic DNA. Translation: BAC08035.1.
RefSeqiNP_681273.1. NC_004113.1.
WP_011056334.1. NC_004113.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SUYNMR-C/D488-518[»]
1SV1NMR-C/D488-518[»]
4O0MX-ray2.84A/B/C1-518[»]
ProteinModelPortaliQ79V60.
SMRiQ79V60.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29357N.
IntActiQ79V60. 2 interactors.
MINTiMINT-1954047.
STRINGi197221.tlr0483.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC08035; BAC08035; BAC08035.
GeneIDi1012389.
KEGGitel:tlr0483.
PATRICi23926210. VBITheElo119873_0508.

Phylogenomic databases

eggNOGiENOG4105EHU. Bacteria.
COG0467. LUCA.
HOGENOMiHOG000230416.
KOiK08482.
OMAiICAYPES.
OrthoDBiPOG091H0SAA.

Miscellaneous databases

EvolutionaryTraceiQ79V60.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01836. KaiC. 1 hit.
InterProiIPR013503. Circadian_KaiC_bact.
IPR030665. KaiC.
IPR014774. KaiC-like_dom.
IPR010624. KaiC_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF06745. ATPase. 2 hits.
[Graphical view]
PIRSFiPIRSF039117. KaiC. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR02655. circ_KaiC. 1 hit.
PROSITEiPS51146. KAIC. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAIC_THEEB
AccessioniPrimary (citable) accession number: Q79V60
Secondary accession number(s): Q8RR33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.