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Protein

Circadian clock protein KaiA

Gene

kaiA

Organism
Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction. In the complex, it enhances the phosphorylation status of KaiC. In contrast, the presence of KaiB in the complex decreases the phosphorylation status of KaiC, suggesting that KaiB acts by antagonizing the interaction between KaiA and KaiC. A KaiA dimer is sufficient to enhance KaiC hexamer phosphorylation.4 Publications

Miscellaneous

'Kai' means 'cycle' in Japanese.

GO - Molecular functioni

  • identical protein binding Source: IntAct

GO - Biological processi

  • circadian rhythm Source: InterPro
  • entrainment of circadian clock Source: UniProtKB
  • negative regulation of protein dephosphorylation Source: CACAO
  • positive regulation of circadian rhythm Source: UniProtKB
  • protein phosphorylation Source: InterPro

Keywordsi

Biological processBiological rhythms

Enzyme and pathway databases

BioCyciSYNEL:SYNPCC7942_1218-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Circadian clock protein KaiA
Gene namesi
Name:kaiA
Ordered Locus Names:Synpcc7942_1218
ORF Names:see0009
OrganismiSynechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2)
Taxonomic identifieri1140 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
Proteomesi
  • UP000002717 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi9I → T: Extends the period of the circadian rhythm to 29 hours. 1 Publication1
Mutagenesisi16I → F: Extends the period of the circadian rhythm to 27 hours. 1 Publication1
Mutagenesisi31L → P: Extends the period of the circadian rhythm to 27 hours. 1 Publication1
Mutagenesisi36S → P: Extends the period of the circadian rhythm to 29 hours. 1 Publication1
Mutagenesisi53C → S: Induces an arrhythmic phenotype. 1 Publication1
Mutagenesisi76V → G: Extends the period of the circadian rhythm to 28 hours. 1 Publication1
Mutagenesisi103E → K in kaiA1; extends the period of the circadian rhythm to 33 hours and increases the interaction with KaiB. 1
Mutagenesisi113Q → R: Extends the period of the circadian rhythm to 33 hours. 1 Publication1
Mutagenesisi117Q → L: Extends the period of the circadian rhythm to 26 hours. 1 Publication1
Mutagenesisi119D → E: Extends the period of the circadian rhythm to 30 hours. 1 Publication1
Mutagenesisi119D → G: Extends the period of the circadian rhythm to 26 hours. 1 Publication1
Mutagenesisi131V → A: Extends the period of the circadian rhythm to 28 hours. 1 Publication1
Mutagenesisi136D → V: Extends the period of the circadian rhythm to 30 hours. 1 Publication1
Mutagenesisi136D → Y: Extends the period of the circadian rhythm to 29 hours. 1 Publication1
Mutagenesisi166Y → C in kaiA3; induces an arrhythmic phenotype and reduces the interaction with KaiC. 1
Mutagenesisi178F → S or I: Induces an arrhythmic phenotype. 1 Publication1
Mutagenesisi180R → H: Extends the period of the circadian rhythm to 26 hours. 1 Publication1
Mutagenesisi194M → T: Extends the period of the circadian rhythm to 26 hours. 1 Publication1
Mutagenesisi224F → S: Induces an arrhythmic phenotype. 1 Publication1
Mutagenesisi225F → S: Induces an arrhythmic phenotype. 1 Publication1
Mutagenesisi239E → G: Extends the period of the circadian rhythm to 28 hours. 1 Publication1
Mutagenesisi241M → T: Extends the period of the circadian rhythm to 38 hours. 1 Publication1
Mutagenesisi242D → V or G: Extends the period of the circadian rhythm to 27 hours. 1 Publication1
Mutagenesisi243E → A: Extends the period of the circadian rhythm to 34 hours. 1 Publication1
Mutagenesisi244F → V: Extends the period of the circadian rhythm to 26 hours. 1 Publication1
Mutagenesisi245A → D: Extends the period of the circadian rhythm to 26 hours. 1 Publication1
Mutagenesisi249R → H in kaiA1; extends the period of the circadian rhythm to 30 hours. 1
Mutagenesisi266I → V: Extends the period of the circadian rhythm to 27 hours. 1 Publication1
Mutagenesisi273C → Y: Extends the period of the circadian rhythm to 30 hours. 1 Publication1
Mutagenesisi274E → K in kaiA3; induces an arrhythmic phenotype. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002178711 – 284Circadian clock protein KaiAAdd BLAST284

Proteomic databases

PRIDEiQ79PF6.

Expressioni

Developmental stagei

Expressed constantly throughout the circadian cycle.1 Publication

Interactioni

Subunit structurei

Homodimer. Component of the KaiABC complex, at least composed of a KaiC homohexamer, a KaiB dimer and two KaiA dimers. The KaiABC complex also interacts with SasA.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-33329N.
IntActiQ79PF6. 4 interactors.
STRINGi1140.Synpcc7942_1218.

Structurei

Secondary structure

1284
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 10Combined sources6
Helixi14 – 23Combined sources10
Beta strandi29 – 34Combined sources6
Helixi37 – 46Combined sources10
Turni47 – 49Combined sources3
Beta strandi52 – 57Combined sources6
Helixi63 – 72Combined sources10
Beta strandi79 – 83Combined sources5
Beta strandi87 – 89Combined sources3
Beta strandi98 – 100Combined sources3
Beta strandi104 – 106Combined sources3
Helixi108 – 110Combined sources3
Helixi111 – 113Combined sources3
Helixi114 – 128Combined sources15
Turni134 – 136Combined sources3
Beta strandi138 – 140Combined sources3
Turni144 – 147Combined sources4
Helixi149 – 151Combined sources3
Helixi152 – 164Combined sources13
Beta strandi165 – 171Combined sources7
Helixi175 – 177Combined sources3
Turni179 – 181Combined sources3
Helixi184 – 205Combined sources22
Beta strandi208 – 211Combined sources4
Helixi212 – 225Combined sources14
Helixi230 – 249Combined sources20
Turni250 – 252Combined sources3
Helixi257 – 261Combined sources5
Helixi262 – 279Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M2ENMR-A1-135[»]
1M2FNMR-A1-135[»]
1R8JX-ray2.03A/B2-284[»]
4G86X-ray2.39A/B2-284[»]
5C5EX-ray2.82A/B1-284[»]
5N8Yelectron microscopy4.70M/N/O/P/Q/R/S/T/U/V/W/X1-284[»]
ProteinModelPortaliQ79PF6.
SMRiQ79PF6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ79PF6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 164KaiA N-terminalPROSITE-ProRule annotationAdd BLAST164
Domaini174 – 282KaiA C-terminalPROSITE-ProRule annotationAdd BLAST109

Domaini

The N-terminal part (1-102), which is also called pseudo-receiver domain, does not enhance KaiC autophosphorylation. It may have an amplitude-amplifier function and may be involved in transducing input signals to the KaiABC complex. This domain is not conserved in other species.1 Publication
The KaiA domain mediates the interaction with KaiC, the homodimerization, and is responsible for the clock oscillation function.1 Publication

Phylogenomic databases

eggNOGiENOG4108WXF. Bacteria.
ENOG410YS5X. LUCA.
HOGENOMiHOG000276784.
KOiK08480.
OrthoDBiPOG091H10AH.

Family and domain databases

Gene3Di1.10.1240.30. 1 hit.
InterProiView protein in InterPro
IPR011006. CheY-like_superfamily.
IPR011648. Circadian_clock_KaiA.
IPR020844. Circadian_clock_KaiA_N.
IPR020856. Circadian_clock_protein_KaiA_C.
IPR017944. KaiA/RbsU_helical_domain_sf.
PfamiView protein in Pfam
PF07688. KaiA. 1 hit.
SMARTiView protein in SMART
SM01247. KaiA. 1 hit.
SUPFAMiSSF101215. SSF101215. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiView protein in PROSITE
PS51431. KAIA_C. 1 hit.
PS51430. KAIA_N. 1 hit.

Sequencei

Sequence statusi: Complete.

Q79PF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSQIAICIW VESTAILQDC QRALSADRYQ LQVCESGEML LEYAQTHRDQ
60 70 80 90 100
IDCLILVAAN PSFRAVVQQL CFEGVVVPAI VVGDRDSEDP DEPAKEQLYH
110 120 130 140 150
SAELHLGIHQ LEQLPYQVDA ALAEFLRLAP VETMADHIML MGANHDPELS
160 170 180 190 200
SQQRDLAQRL QERLGYLGVY YKRDPDRFLR NLPAYESQKL HQAMQTSYRE
210 220 230 240 250
IVLSYFSPNS NLNQSIDNFV NMAFFADVPV TKVVEIHMEL MDEFAKKLRV
260 270 280
EGRSEDILLD YRLTLIDVIA HLCEMYRRSI PRET
Length:284
Mass (Da):32,632
Last modified:July 5, 2004 - v1
Checksum:i7C4F3B79FFDF59A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010691 Genomic DNA. Translation: BAA37101.1.
AY120853 Genomic DNA. Translation: AAM82684.1.
CP000100 Genomic DNA. Translation: ABB57248.1.
PIRiT44267.
RefSeqiWP_011377921.1. NC_007604.1.

Genome annotation databases

EnsemblBacteriaiABB57248; ABB57248; Synpcc7942_1218.
KEGGisyf:Synpcc7942_1218.

Similar proteinsi

Entry informationi

Entry nameiKAIA_SYNE7
AccessioniPrimary (citable) accession number: Q79PF6
Secondary accession number(s): Q31NX1, Q9Z3H4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 5, 2004
Last modified: November 22, 2017
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

PubMed:15007067 structure is derived from this sequence and not from the one of S.elongatus.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references