Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q79MP6

- Q79MP6_PSEAI

UniProt

Q79MP6 - Q79MP6_PSEAI

Protein

Beta-lactamase

Gene

blaIMP-1

Organism
Pseudomonas aeruginosa
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.UniRule annotation

    Cofactori

    Binds 2 zinc ions per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi52 – 521Zinc 1; via tele nitrogenImported
    Metal bindingi95 – 951Zinc 2; via tele nitrogenImported
    Metal bindingi97 – 971Zinc 2; via pros nitrogenImported
    Metal bindingi99 – 991Zinc 3Imported
    Metal bindingi157 – 1571Zinc 2; via tele nitrogenImported
    Metal bindingi176 – 1761Zinc 3Imported
    Metal bindingi215 – 2151Zinc 3; via tele nitrogenImported
    Metal bindingi217 – 2171Zinc 1Imported

    GO - Molecular functioni

    1. beta-lactamase activity Source: InterPro
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. antibiotic catabolic process Source: InterPro

    Keywords - Molecular functioni

    HydrolaseUniRule annotation

    Keywords - Biological processi

    Antibiotic resistanceUniRule annotation

    Keywords - Ligandi

    Metal-bindingUniRule annotationImported, ZincUniRule annotationImported

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamaseUniRule annotation (EC:3.5.2.6UniRule annotation)
    Gene namesi
    Name:blaIMP-1Imported
    Synonyms:bla IMPImported, bla-impImported, blaESPImported, impImported
    Encoded oniPlasmid pMS350Imported
    OrganismiPseudomonas aeruginosaImported
    Taxonomic identifieri287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DDKX-ray3.10A20-239[»]
    1JJEX-ray1.80A/B19-240[»]
    1JJTX-ray1.80A/B19-246[»]
    4F6HX-ray1.74A19-246[»]
    4F6ZX-ray2.00A19-246[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ79MP6.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the metallo-beta-lactamase superfamily. Class-B beta-lactamase family.UniRule annotation

    Family and domain databases

    Gene3Di3.60.15.10. 1 hit.
    InterProiIPR001279. Beta-lactamas-like.
    IPR001018. Beta-lactamase_class-B_CS.
    [Graphical view]
    PfamiPF00753. Lactamase_B. 1 hit.
    [Graphical view]
    SMARTiSM00849. Lactamase_B. 1 hit.
    [Graphical view]
    SUPFAMiSSF56281. SSF56281. 1 hit.
    PROSITEiPS00743. BETA_LACTAMASE_B_1. 1 hit.
    PS00744. BETA_LACTAMASE_B_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q79MP6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKLSVFFIF LFCSIATAAE SLPDLKIEKL DEGVYVHTSF EEVNGWGVVP    50
    KHGLVVLVNA EAYLIDTPFT AKDTEKLVTW FVERGYKIKG SISSHFHSDS 100
    TGGIEWLNSR SIPTYASELT NELLKKDGKV QATNSFSGVN YWLVKNKIEV 150
    FYPGPGHTPD NVVVWLPERK ILFGGCFIKP YGLGNLGDAN IEAWPKSAKL 200
    LKSKYGKAKL VVPSHSEVGD ASLLKLTLEQ AVKGLNESKK PSKPSN 246
    Length:246
    Mass (Da):27,120
    Last modified:July 5, 2004 - v1
    Checksum:i9B2599E8F1B22D36
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei246 – 2461Imported

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY168635 Genomic DNA. Translation: AAN87168.1.
    AY625686 Genomic DNA. Translation: AAT48073.1.
    AY625687 Genomic DNA. Translation: AAT48074.1.
    AY625688 Genomic DNA. Translation: AAT48075.1.
    AY625689 Genomic DNA. Translation: AAT48076.1.
    DQ842025 Genomic DNA. Translation: ABG67754.1.
    GU831546 Genomic DNA. Translation: ADF36530.1.
    GU831547 Genomic DNA. Translation: ADF36531.1.
    GU831548 Genomic DNA. Translation: ADF36532.1.
    GU831549 Genomic DNA. Translation: ADF36533.1.
    GU831550 Genomic DNA. Translation: ADF36534.1.
    GU831551 Genomic DNA. Translation: ADF36535.1.
    GU831552 Genomic DNA. Translation: ADF36536.1.
    GU831553 Genomic DNA. Translation: ADF36537.1.
    D78375 Genomic DNA. Translation: BAA23768.1.
    AB104852 Genomic DNA. Translation: BAD14385.1.
    AB462903 Genomic DNA. Translation: BAH66387.1.
    AB472901 Genomic DNA. Translation: BAN78520.1.
    AB901035 Genomic DNA. Translation: BAO48002.1.
    AB901036 Genomic DNA. Translation: BAO48007.1.
    AB901038 Genomic DNA. Translation: BAO48013.1.
    AB901043 Genomic DNA. Translation: BAO48031.1.
    AB901045 Genomic DNA. Translation: BAO48035.1.
    AJ223604 Genomic DNA. Translation: CAA11471.1.
    X98393 Genomic DNA. Translation: CAA67040.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY168635 Genomic DNA. Translation: AAN87168.1 .
    AY625686 Genomic DNA. Translation: AAT48073.1 .
    AY625687 Genomic DNA. Translation: AAT48074.1 .
    AY625688 Genomic DNA. Translation: AAT48075.1 .
    AY625689 Genomic DNA. Translation: AAT48076.1 .
    DQ842025 Genomic DNA. Translation: ABG67754.1 .
    GU831546 Genomic DNA. Translation: ADF36530.1 .
    GU831547 Genomic DNA. Translation: ADF36531.1 .
    GU831548 Genomic DNA. Translation: ADF36532.1 .
    GU831549 Genomic DNA. Translation: ADF36533.1 .
    GU831550 Genomic DNA. Translation: ADF36534.1 .
    GU831551 Genomic DNA. Translation: ADF36535.1 .
    GU831552 Genomic DNA. Translation: ADF36536.1 .
    GU831553 Genomic DNA. Translation: ADF36537.1 .
    D78375 Genomic DNA. Translation: BAA23768.1 .
    AB104852 Genomic DNA. Translation: BAD14385.1 .
    AB462903 Genomic DNA. Translation: BAH66387.1 .
    AB472901 Genomic DNA. Translation: BAN78520.1 .
    AB901035 Genomic DNA. Translation: BAO48002.1 .
    AB901036 Genomic DNA. Translation: BAO48007.1 .
    AB901038 Genomic DNA. Translation: BAO48013.1 .
    AB901043 Genomic DNA. Translation: BAO48031.1 .
    AB901045 Genomic DNA. Translation: BAO48035.1 .
    AJ223604 Genomic DNA. Translation: CAA11471.1 .
    X98393 Genomic DNA. Translation: CAA67040.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DDK X-ray 3.10 A 20-239 [» ]
    1JJE X-ray 1.80 A/B 19-240 [» ]
    1JJT X-ray 1.80 A/B 19-246 [» ]
    4F6H X-ray 1.74 A 19-246 [» ]
    4F6Z X-ray 2.00 A 19-246 [» ]
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL1293244.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q79MP6.

    Family and domain databases

    Gene3Di 3.60.15.10. 1 hit.
    InterProi IPR001279. Beta-lactamas-like.
    IPR001018. Beta-lactamase_class-B_CS.
    [Graphical view ]
    Pfami PF00753. Lactamase_B. 1 hit.
    [Graphical view ]
    SMARTi SM00849. Lactamase_B. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56281. SSF56281. 1 hit.
    PROSITEi PS00743. BETA_LACTAMASE_B_1. 1 hit.
    PS00744. BETA_LACTAMASE_B_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of an enterobacterial metallo beta-lactamase found in a clinical isolate of Serratia marcescens that shows imipenem resistance."
      Osano E., Arakawa Y., Wacharotayankun R., Ohta M., Horii T., Ito H., Yoshimura F., Kato N.
      Antimicrob. Agents Chemother. 38:71-78(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Plasmid: pMS350
    2. "Insertion of a carbapenemase gene cassette into an integron of a Pseudomonas aeruginosa plasmid."
      Iyobe S., Minami S., Yamada H.
      J. Antimicrob. Chemother. 38:1114-1115(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Plasmid: pMS350
    3. Laraki N., Rossolini G., Galleni M., Frere J.M.
      Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
    4. Galleni M.G.
      Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
    5. "Structure of In31, a blaIMP-containing Pseudomonas aeruginosa integron phyletically related to In5, which carries an unusual array of gene cassettes."
      Laraki N., Galleni M., Thamm I., Riccio M.L., Amicosante G., Frere J.M., Rossolini G.M.
      Antimicrob. Agents Chemother. 43:890-901(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    6. "Crystal structure of the IMP-1 metallo beta-lactamase from Pseudomonas aeruginosa and its complex with a mercaptocarboxylate inhibitor: binding determinants of a potent, broad-spectrum inhibitor."
      Concha N.O., Janson C.A., Rowling P., Pearson S., Cheever C.A., Clarke B.P., Lewis C., Galleni M., Frere J.M., Payne D.J., Bateson J.H., Abdel-Meguid S.S.
      Biochemistry 39:4288-4298(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS) OF 20-239 IN COMPLEX WITH ZINC.
    7. Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 19-240 IN COMPLEX WITH ZINC.
    8. "Clonal spread of IMP-1-producing Pseudomonas aeruginosa in two hospitals in Singapore."
      Koh T.H., Wang G.C., Sng L.H.
      J. Clin. Microbiol. 42:5378-5380(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: DM14158/00Imported, DU10114/00Imported, DU32495/00Imported, DU40799/00Imported and DU8622/00Imported.
    9. "Multidrug-resistant Pseudomonas aeruginosa strain that caused an outbreak in a neurosurgery ward and its aac(6')-Iae gene cassette encoding a novel aminoglycoside acetyltransferase."
      Sekiguchi J., Asagi T., Miyoshi-Akiyama T., Fujino T., Kobayashi I., Morita K., Kikuchi Y., Kuratsuji T., Kirikae T.
      Antimicrob. Agents Chemother. 49:3734-3742(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: IMCJ2.S1Imported.
    10. "Molecular epidemiology of clinical Pseudomonas aeruginosa isolates carrying IMP-1 metallo-beta-lactamase gene in a University Hospital in Turkey."
      Ozgumus O.B., Caylan R., Tosun I., Sandalli C., Aydin K., Koksal I.
      Microb. Drug Resist. 13:191-198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    11. Kitao-Ando T., Miyoshi-Akiyama T., Kirikae T.
      Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: IMCJ798Imported.
    12. "AAC(6')-Iaf, a novel aminoglycoside 6'-N-acetyltransferase from multidrug-resistant Pseudomonas aeruginosa clinical isolates."
      Kitao T., Miyoshi-Akiyama T., Kirikae T.
      Antimicrob. Agents Chemother. 53:2327-2334(2009)
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: IMCJ798Imported.
    13. Polotto M., Nogueira M.L., Nogueira M.C.L.
      Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: PHB 2Imported, PHB 3Imported, PHB 49Imported, PHB 51Imported, PHB 53Imported, PHB 59Imported, PHB 60Imported and PHB 62Imported.
    14. "Mutagenesis of zinc ligand residue Cys221 reveals plasticity in the IMP-1 metallo-?-lactamase active site."
      Horton L.B., Shanker S., Mikulski R., Brown N.G., Phillips K.J., Lykissa E., Venkataram Prasad B.V., Palzkill T.
      Antimicrob. Agents Chemother. 56:5667-5677(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.74 ANGSTROMS) OF 19-246 IN COMPLEX WITH ZINC.
    15. "Identification and Characterization of a Novel aac(6')-Iag Associated with the bla IMP-1-Integron in a Multidrug-Resistant Pseudomonas aeruginosa."
      Kobayashi K., Hayashi I., Kouda S., Kato F., Fujiwara T., Kayama S., Hirakawa H., Itaha H., Ohge H., Gotoh N., Usui T., Matsubara A., Sugai M.
      PLoS ONE 8:e70557-e70557(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: NK0009Imported.
    16. "Diversity of metallo-beta-lactamase genes and integron structures among clinical isolates of Pseudomonas aeruginosa in Japan."
      Mano Y., Ishii Y., Yoshizumi Y., Saga T., Furuya N., Yamaguchi K., Tateda K.
      Submitted (JAN-2014) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: TUM3982Imported, TUM3987Imported, TUM3992Imported, TUM4027Imported and TUM4032Imported.

    Entry informationi

    Entry nameiQ79MP6_PSEAI
    AccessioniPrimary (citable) accession number: Q79MP6
    Entry historyi
    Integrated into UniProtKB/TrEMBL: July 5, 2004
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, PlasmidImported

    External Data

    Dasty 3