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Q79MP6

- Q79MP6_PSEAI

UniProt

Q79MP6 - Q79MP6_PSEAI

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Protein
Beta-lactamase
Gene
blaIMP-1, bla IMP, bla-imp, blaESP, imp
Organism
Pseudomonas aeruginosa
Status
Unreviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.UniRule annotation

Cofactori

Binds 2 zinc ions per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi52 – 521Zinc 1; via tele nitrogenImported
Metal bindingi95 – 951Zinc 2; via tele nitrogenImported
Metal bindingi97 – 971Zinc 2; via pros nitrogenImported
Metal bindingi99 – 991Zinc 3
Binding sitei142 – 1421Phosphate 1Imported
Binding sitei149 – 1491Phosphate 1Imported
Metal bindingi157 – 1571Zinc 2; via tele nitrogenImported
Metal bindingi176 – 1761Zinc 3
Metal bindingi215 – 2151Zinc 3; via tele nitrogen
Metal bindingi217 – 2171Zinc 1Imported

GO - Molecular functioni

  1. beta-lactamase activity Source: InterPro
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. antibiotic catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotation

Keywords - Biological processi

Antibiotic resistanceUniRule annotation

Keywords - Ligandi

Metal-bindingUniRule annotationImported, ZincUniRule annotationImported

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamaseUniRule annotation (EC:3.5.2.6UniRule annotation)
Gene namesi
Name:blaIMP-1Imported
Synonyms:bla IMPImported, bla-impImported, blaESPImported, impImported
Encoded oniPlasmid pMS350Imported
OrganismiPseudomonas aeruginosaImported
Taxonomic identifieri287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DDKX-ray3.10A20-239[»]
1JJEX-ray1.80A/B19-240[»]
1JJTX-ray1.80A/B19-246[»]
4F6HX-ray1.74A19-246[»]
4F6ZX-ray2.00A19-246[»]

Miscellaneous databases

EvolutionaryTraceiQ79MP6.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni178 – 1792Phosphate 2 bindingImported
Regioni215 – 2162Phosphate 2 bindingImported

Sequence similaritiesi

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR001279. Beta-lactamas-like.
IPR001018. Beta-lactamase_class-B_CS.
[Graphical view]
PfamiPF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
PROSITEiPS00743. BETA_LACTAMASE_B_1. 1 hit.
PS00744. BETA_LACTAMASE_B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q79MP6-1 [UniParc]FASTAAdd to Basket

« Hide

MSKLSVFFIF LFCSIATAAE SLPDLKIEKL DEGVYVHTSF EEVNGWGVVP    50
KHGLVVLVNA EAYLIDTPFT AKDTEKLVTW FVERGYKIKG SISSHFHSDS 100
TGGIEWLNSR SIPTYASELT NELLKKDGKV QATNSFSGVN YWLVKNKIEV 150
FYPGPGHTPD NVVVWLPERK ILFGGCFIKP YGLGNLGDAN IEAWPKSAKL 200
LKSKYGKAKL VVPSHSEVGD ASLLKLTLEQ AVKGLNESKK PSKPSN 246
Length:246
Mass (Da):27,120
Last modified:July 5, 2004 - v1
Checksum:i9B2599E8F1B22D36
GO

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei246 – 2461Imported

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY168635 Genomic DNA. Translation: AAN87168.1.
AY625686 Genomic DNA. Translation: AAT48073.1.
AY625687 Genomic DNA. Translation: AAT48074.1.
AY625688 Genomic DNA. Translation: AAT48075.1.
AY625689 Genomic DNA. Translation: AAT48076.1.
DQ842025 Genomic DNA. Translation: ABG67754.1.
GU831546 Genomic DNA. Translation: ADF36530.1.
GU831547 Genomic DNA. Translation: ADF36531.1.
GU831548 Genomic DNA. Translation: ADF36532.1.
GU831549 Genomic DNA. Translation: ADF36533.1.
GU831550 Genomic DNA. Translation: ADF36534.1.
GU831551 Genomic DNA. Translation: ADF36535.1.
GU831552 Genomic DNA. Translation: ADF36536.1.
GU831553 Genomic DNA. Translation: ADF36537.1.
D78375 Genomic DNA. Translation: BAA23768.1.
AB104852 Genomic DNA. Translation: BAD14385.1.
AB462903 Genomic DNA. Translation: BAH66387.1.
AB472901 Genomic DNA. Translation: BAN78520.1.
AB901035 Genomic DNA. Translation: BAO48002.1.
AB901036 Genomic DNA. Translation: BAO48007.1.
AB901038 Genomic DNA. Translation: BAO48013.1.
AB901043 Genomic DNA. Translation: BAO48031.1.
AB901045 Genomic DNA. Translation: BAO48035.1.
AJ223604 Genomic DNA. Translation: CAA11471.1.
X98393 Genomic DNA. Translation: CAA67040.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY168635 Genomic DNA. Translation: AAN87168.1 .
AY625686 Genomic DNA. Translation: AAT48073.1 .
AY625687 Genomic DNA. Translation: AAT48074.1 .
AY625688 Genomic DNA. Translation: AAT48075.1 .
AY625689 Genomic DNA. Translation: AAT48076.1 .
DQ842025 Genomic DNA. Translation: ABG67754.1 .
GU831546 Genomic DNA. Translation: ADF36530.1 .
GU831547 Genomic DNA. Translation: ADF36531.1 .
GU831548 Genomic DNA. Translation: ADF36532.1 .
GU831549 Genomic DNA. Translation: ADF36533.1 .
GU831550 Genomic DNA. Translation: ADF36534.1 .
GU831551 Genomic DNA. Translation: ADF36535.1 .
GU831552 Genomic DNA. Translation: ADF36536.1 .
GU831553 Genomic DNA. Translation: ADF36537.1 .
D78375 Genomic DNA. Translation: BAA23768.1 .
AB104852 Genomic DNA. Translation: BAD14385.1 .
AB462903 Genomic DNA. Translation: BAH66387.1 .
AB472901 Genomic DNA. Translation: BAN78520.1 .
AB901035 Genomic DNA. Translation: BAO48002.1 .
AB901036 Genomic DNA. Translation: BAO48007.1 .
AB901038 Genomic DNA. Translation: BAO48013.1 .
AB901043 Genomic DNA. Translation: BAO48031.1 .
AB901045 Genomic DNA. Translation: BAO48035.1 .
AJ223604 Genomic DNA. Translation: CAA11471.1 .
X98393 Genomic DNA. Translation: CAA67040.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DDK X-ray 3.10 A 20-239 [» ]
1JJE X-ray 1.80 A/B 19-240 [» ]
1JJT X-ray 1.80 A/B 19-246 [» ]
4F6H X-ray 1.74 A 19-246 [» ]
4F6Z X-ray 2.00 A 19-246 [» ]
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL1293244.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q79MP6.

Family and domain databases

Gene3Di 3.60.15.10. 1 hit.
InterProi IPR001279. Beta-lactamas-like.
IPR001018. Beta-lactamase_class-B_CS.
[Graphical view ]
Pfami PF00753. Lactamase_B. 1 hit.
[Graphical view ]
SMARTi SM00849. Lactamase_B. 1 hit.
[Graphical view ]
SUPFAMi SSF56281. SSF56281. 1 hit.
PROSITEi PS00743. BETA_LACTAMASE_B_1. 1 hit.
PS00744. BETA_LACTAMASE_B_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of an enterobacterial metallo beta-lactamase found in a clinical isolate of Serratia marcescens that shows imipenem resistance."
    Osano E., Arakawa Y., Wacharotayankun R., Ohta M., Horii T., Ito H., Yoshimura F., Kato N.
    Antimicrob. Agents Chemother. 38:71-78(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Plasmid: pMS350
  2. "Insertion of a carbapenemase gene cassette into an integron of a Pseudomonas aeruginosa plasmid."
    Iyobe S., Minami S., Yamada H.
    J. Antimicrob. Chemother. 38:1114-1115(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Plasmid: pMS350
  3. Laraki N., Rossolini G., Galleni M., Frere J.M.
    Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  4. Galleni M.G.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  5. "Structure of In31, a blaIMP-containing Pseudomonas aeruginosa integron phyletically related to In5, which carries an unusual array of gene cassettes."
    Laraki N., Galleni M., Thamm I., Riccio M.L., Amicosante G., Frere J.M., Rossolini G.M.
    Antimicrob. Agents Chemother. 43:890-901(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  6. "Crystal structure of the IMP-1 metallo beta-lactamase from Pseudomonas aeruginosa and its complex with a mercaptocarboxylate inhibitor: binding determinants of a potent, broad-spectrum inhibitor."
    Concha N.O., Janson C.A., Rowling P., Pearson S., Cheever C.A., Clarke B.P., Lewis C., Galleni M., Frere J.M., Payne D.J., Bateson J.H., Abdel-Meguid S.S.
    Biochemistry 39:4288-4298(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS) OF 20-239 IN COMPLEX WITH ZINC.
  7. Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 19-240 IN COMPLEX WITH ZINC.
  8. "Clonal spread of IMP-1-producing Pseudomonas aeruginosa in two hospitals in Singapore."
    Koh T.H., Wang G.C., Sng L.H.
    J. Clin. Microbiol. 42:5378-5380(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DM14158/00Imported, DU10114/00Imported, DU32495/00Imported, DU40799/00Imported and DU8622/00Imported.
  9. "Multidrug-resistant Pseudomonas aeruginosa strain that caused an outbreak in a neurosurgery ward and its aac(6')-Iae gene cassette encoding a novel aminoglycoside acetyltransferase."
    Sekiguchi J., Asagi T., Miyoshi-Akiyama T., Fujino T., Kobayashi I., Morita K., Kikuchi Y., Kuratsuji T., Kirikae T.
    Antimicrob. Agents Chemother. 49:3734-3742(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: IMCJ2.S1Imported.
  10. "Molecular epidemiology of clinical Pseudomonas aeruginosa isolates carrying IMP-1 metallo-beta-lactamase gene in a University Hospital in Turkey."
    Ozgumus O.B., Caylan R., Tosun I., Sandalli C., Aydin K., Koksal I.
    Microb. Drug Resist. 13:191-198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  11. Kitao-Ando T., Miyoshi-Akiyama T., Kirikae T.
    Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: IMCJ798Imported.
  12. "AAC(6')-Iaf, a novel aminoglycoside 6'-N-acetyltransferase from multidrug-resistant Pseudomonas aeruginosa clinical isolates."
    Kitao T., Miyoshi-Akiyama T., Kirikae T.
    Antimicrob. Agents Chemother. 53:2327-2334(2009)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: IMCJ798Imported.
  13. Polotto M., Nogueira M.L., Nogueira M.C.L.
    Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: PHB 2Imported, PHB 3Imported, PHB 49Imported, PHB 51Imported, PHB 53Imported, PHB 59Imported, PHB 60Imported and PHB 62Imported.
  14. "Mutagenesis of zinc ligand residue Cys221 reveals plasticity in the IMP-1 metallo-?-lactamase active site."
    Horton L.B., Shanker S., Mikulski R., Brown N.G., Phillips K.J., Lykissa E., Venkataram Prasad B.V., Palzkill T.
    Antimicrob. Agents Chemother. 56:5667-5677(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.74 ANGSTROMS) OF 19-246 IN COMPLEX WITH PHOSPHATE AND ZINC.
  15. "Identification and Characterization of a Novel aac(6')-Iag Associated with the bla IMP-1-Integron in a Multidrug-Resistant Pseudomonas aeruginosa."
    Kobayashi K., Hayashi I., Kouda S., Kato F., Fujiwara T., Kayama S., Hirakawa H., Itaha H., Ohge H., Gotoh N., Usui T., Matsubara A., Sugai M.
    PLoS ONE 8:e70557-e70557(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: NK0009Imported.
  16. "Diversity of metallo-beta-lactamase genes and integron structures among clinical isolates of Pseudomonas aeruginosa in Japan."
    Mano Y., Ishii Y., Yoshizumi Y., Saga T., Furuya N., Yamaguchi K., Tateda K.
    Submitted (JAN-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: TUM3982Imported, TUM3987Imported, TUM3992Imported, TUM4027Imported and TUM4032Imported.

Entry informationi

Entry nameiQ79MP6_PSEAI
AccessioniPrimary (citable) accession number: Q79MP6
Entry historyi
Integrated into UniProtKB/TrEMBL: July 5, 2004
Last sequence update: July 5, 2004
Last modified: September 3, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported, PlasmidImported

External Data

Dasty 3

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