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Q79LY0

- HOPD2_PSESM

UniProt

Q79LY0 - HOPD2_PSESM

Protein

Effector protein hopD2

Gene

hopD2

Organism
Pseudomonas syringae pv. tomato (strain DC3000)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Effector showing tyrosine-phosphatase activity required for host defense suppression. Functions inside plant cells causing suppression of HR (hypersensitive response), PR1 gene expression and oxidative burst probably by interfering with a MAPK (mitogen-activated protein kinase) pathway. MAPK cascades are known to activate defense-related transcription factors. Inhibits plant pattern-recognition receptors (PRRs) activation.3 Publications

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotations

    Enzyme regulationi

    Inhibited by sodium orthovanadate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei378 – 3781Phosphocysteine intermediate

    GO - Molecular functioni

    1. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. modulation by symbiont of host defense-related programmed cell death Source: UniProtKB-KW
    2. pathogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Hypersensitive response elicitation, Virulence

    Enzyme and pathway databases

    BioCyciPSYR223283:GJIX-4787-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Effector protein hopD2 (EC:3.1.3.48)
    Alternative name(s):
    Tyrosine-protein phosphatase hopPtoD2
    Gene namesi
    Name:hopD2
    Synonyms:hopA01, hopPtoD2
    Ordered Locus Names:PSPTO_4722
    OrganismiPseudomonas syringae pv. tomato (strain DC3000)
    Taxonomic identifieri223283 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    Subcellular locationi

    Secreted 1 Publication
    Note: Secreted via type III secretion system (TTSS).

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi378 – 3781C → S: Loss of phosphatase activity and decreased inhibition of EFR phosphorylation. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 468468Effector protein hopD2PRO_0000094863Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Inductioni

    Transcriptionally induced by HrpL.2 Publications

    Interactioni

    Subunit structurei

    Interacts with EFR and FLS2 (via the kinase and cytoplasmic domains).1 Publication

    Protein-protein interaction databases

    STRINGi223283.PSPTO_4722.

    Structurei

    3D structure databases

    ProteinModelPortaliQ79LY0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini143 – 468326Tyrosine-protein phosphataseAdd
    BLAST

    Domaini

    The N-terminal domain (residues 1-142) retains 70% identity to the N-terminal 142 residues of AvrPphD, and is required for translocation into plant cells via TTSS.1 Publication

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG14544.
    OMAiRESAMIT.
    OrthoDBiEOG68WR39.

    Family and domain databases

    Gene3Di3.90.190.10. 2 hits.
    InterProiIPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 2 hits.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Sequencei

    Sequence statusi: Complete.

    Q79LY0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNPLQPIQHS ITNSQMSGGQ QLEAEGSQAH NSYSHPDRIS LSQLSQSAHL    50
    ALDHLSTQPN TDHQRVASLV RNAVQDGKFQ LQSSNDTQVT YKTSVCPPAN 100
    ADTMGAAHLI NNELTVQARL NDQLEYDIVS AHLYGPSEAI SIDASSPPSA 150
    NDLASSGLSE RTHLGMNRVL LRYAVPPRET EDQCVMVIDK MPPPKHGKMS 200
    FFRTTNDLSK LPLGMETGGL SDLKLAGCER ISSVEQVKSI RAALGGGPLT 250
    VLDLREESHA IVNGLPITLR GPMDWANAGL SQVDGAARES AMITELKRTK 300
    SLTLVDANYV KGKKSNPQTT ELKNLNVRSE REVVTEAGAT YRRVAITDHN 350
    RPSPEATDEL VDIMRHCLQA NESLVVHCNG GRGRTTTAMI MVDMLKNARN 400
    HSAETLITRM AKLSYDYNMT DLGSISALKR PFLEDRLKFL QAFHDYARNN 450
    PSGLSLNWTQ WRAKIALE 468
    Length:468
    Mass (Da):51,380
    Last modified:July 5, 2004 - v1
    Checksum:i351D7B79664F9E81
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF469470 Genomic DNA. Translation: AAO33450.1.
    AY198373 Genomic DNA. Translation: AAO43976.1.
    AE016853 Genomic DNA. Translation: AAO58160.1.
    RefSeqiNP_794465.1. NC_004578.1.

    Genome annotation databases

    EnsemblBacteriaiAAO58160; AAO58160; PSPTO_4722.
    GeneIDi1186405.
    KEGGipst:PSPTO_4722.
    PATRICi20000952. VBIPseSyr93040_4834.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF469470 Genomic DNA. Translation: AAO33450.1 .
    AY198373 Genomic DNA. Translation: AAO43976.1 .
    AE016853 Genomic DNA. Translation: AAO58160.1 .
    RefSeqi NP_794465.1. NC_004578.1.

    3D structure databases

    ProteinModelPortali Q79LY0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 223283.PSPTO_4722.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO58160 ; AAO58160 ; PSPTO_4722 .
    GeneIDi 1186405.
    KEGGi pst:PSPTO_4722.
    PATRICi 20000952. VBIPseSyr93040_4834.

    Phylogenomic databases

    eggNOGi NOG14544.
    OMAi RESAMIT.
    OrthoDBi EOG68WR39.

    Enzyme and pathway databases

    BioCyci PSYR223283:GJIX-4787-MONOMER.

    Family and domain databases

    Gene3Di 3.90.190.10. 2 hits.
    InterProi IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 2 hits.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A gene in the Pseudomonas syringae pv. tomato Hrp pathogenicity island conserved effector locus, hopPtoA1, contributes to efficient formation of bacterial colonies in planta and is duplicated elsewhere in the genome."
      Badel J.L., Charkowski A.O., Deng W.-L., Collmer A.
      Mol. Plant Microbe Interact. 15:1014-1024(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DC3000.
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DC3000.
    3. "The Pseudomonas syringae type III-secreted protein HopPtoD2 possesses protein tyrosine phosphatase activity and suppresses programmed cell death in plants."
      Espinosa A., Guo M., Tam V.C., Fu Z.Q., Alfano J.R.
      Mol. Microbiol. 49:377-387(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A PHOSPHATASE, INDUCTION, MUTAGENESIS OF CYS-378.
      Strain: DC3000.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DC3000.
    5. "Genomewide identification of proteins secreted by the Hrp type III protein secretion system of Pseudomonas syringae pv. tomato DC3000."
      Petnicki-Ocwieja T., Schneider D.J., Tam V.C., Chancey S.T., Shan L., Jamir Y., Schechter L.M., Janes M.D., Buell C.R., Tang X., Collmer A., Alfano J.R.
      Proc. Natl. Acad. Sci. U.S.A. 99:7652-7657(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
      Strain: DC3000.
    6. "A translocated protein tyrosine phosphatase of Pseudomonas syringae pv. tomato DC3000 modulates plant defence response to infection."
      Bretz J.R., Mock N.M., Charity J.C., Zeyad S., Baker C.J., Hutcheson S.W.
      Mol. Microbiol. 49:389-400(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A PHOSPHATASE, DOMAIN, INDUCTION.
      Strain: DC3000.
    7. "The Arabidopsis PEPR pathway couples local and systemic plant immunity."
      Ross A., Yamada K., Hiruma K., Yamashita-Yamada M., Lu X., Takano Y., Tsuda K., Saijo Y.
      EMBO J. 33:62-75(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH EFR AND FLS2, MUTAGENESIS OF CYS-378.

    Entry informationi

    Entry nameiHOPD2_PSESM
    AccessioniPrimary (citable) accession number: Q79LY0
    Secondary accession number(s): Q87W44
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    HopPtoD2 is not present in all strains of P.syringae.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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