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Q79LY0 (HOPD2_PSESM) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Effector protein hopD2

EC=3.1.3.48
Alternative name(s):
Tyrosine-protein phosphatase hopPtoD2
Gene names
Name:hopD2
Synonyms:hopA01, hopPtoD2
Ordered Locus Names:PSPTO_4722
OrganismPseudomonas syringae pv. tomato (strain DC3000) [Complete proteome] [HAMAP]
Taxonomic identifier223283 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Effector showing tyrosine-phosphatase activity required for host defense suppression. Functions inside plant cells causing suppression of HR (hypersensitive response), PR1 gene expression and oxidative burst probably by interfering with a MAPK (mitogen-activated protein kinase) pathway. MAPK cascades are known to activate defense-related transcription factors. Inhibits plant pattern-recognition receptors (PRRs) activation. Ref.3 Ref.6 Ref.7

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Enzyme regulation

Inhibited by sodium orthovanadate.

Subunit structure

Interacts with EFR and FLS2 (via the kinase and cytoplasmic domains). Ref.7

Subcellular location

Secreted. Note: Secreted via type III secretion system (TTSS). Ref.5

Induction

Transcriptionally induced by HrpL. Ref.3 Ref.6

Domain

The N-terminal domain (residues 1-142) retains 70% identity to the N-terminal 142 residues of AvrPphD, and is required for translocation into plant cells via TTSS. Ref.6

Miscellaneous

HopPtoD2 is not present in all strains of P.syringae.

Sequence similarities

Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
   Biological processHypersensitive response elicitation
Virulence
   Cellular componentSecreted
   Molecular functionHydrolase
Protein phosphatase
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processmodulation by symbiont of host defense-related programmed cell death

Inferred from electronic annotation. Source: UniProtKB-KW

pathogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein tyrosine phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 468468Effector protein hopD2
PRO_0000094863

Regions

Domain143 – 468326Tyrosine-protein phosphatase

Sites

Active site3781Phosphocysteine intermediate

Experimental info

Mutagenesis3781C → S: Loss of phosphatase activity and decreased inhibition of EFR phosphorylation. Ref.3 Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q79LY0 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 351D7B79664F9E81

FASTA46851,380
        10         20         30         40         50         60 
MNPLQPIQHS ITNSQMSGGQ QLEAEGSQAH NSYSHPDRIS LSQLSQSAHL ALDHLSTQPN 

        70         80         90        100        110        120 
TDHQRVASLV RNAVQDGKFQ LQSSNDTQVT YKTSVCPPAN ADTMGAAHLI NNELTVQARL 

       130        140        150        160        170        180 
NDQLEYDIVS AHLYGPSEAI SIDASSPPSA NDLASSGLSE RTHLGMNRVL LRYAVPPRET 

       190        200        210        220        230        240 
EDQCVMVIDK MPPPKHGKMS FFRTTNDLSK LPLGMETGGL SDLKLAGCER ISSVEQVKSI 

       250        260        270        280        290        300 
RAALGGGPLT VLDLREESHA IVNGLPITLR GPMDWANAGL SQVDGAARES AMITELKRTK 

       310        320        330        340        350        360 
SLTLVDANYV KGKKSNPQTT ELKNLNVRSE REVVTEAGAT YRRVAITDHN RPSPEATDEL 

       370        380        390        400        410        420 
VDIMRHCLQA NESLVVHCNG GRGRTTTAMI MVDMLKNARN HSAETLITRM AKLSYDYNMT 

       430        440        450        460 
DLGSISALKR PFLEDRLKFL QAFHDYARNN PSGLSLNWTQ WRAKIALE 

« Hide

References

« Hide 'large scale' references
[1]"A gene in the Pseudomonas syringae pv. tomato Hrp pathogenicity island conserved effector locus, hopPtoA1, contributes to efficient formation of bacterial colonies in planta and is duplicated elsewhere in the genome."
Badel J.L., Charkowski A.O., Deng W.-L., Collmer A.
Mol. Plant Microbe Interact. 15:1014-1024(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DC3000.
[2]Buell C.R., Berry K.J., Fedorova N.B., Feldblynm T.V., Gwinn M.L., Haft D.H., Khouri H.M., Nelson W.C., Peterson J., Russell D., Tran B., Umayam L., Utterback T.R., Van Aken S.E.
Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DC3000.
[3]"The Pseudomonas syringae type III-secreted protein HopPtoD2 possesses protein tyrosine phosphatase activity and suppresses programmed cell death in plants."
Espinosa A., Guo M., Tam V.C., Fu Z.Q., Alfano J.R.
Mol. Microbiol. 49:377-387(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A PHOSPHATASE, INDUCTION, MUTAGENESIS OF CYS-378.
Strain: DC3000.
[4]"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000."
Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N. expand/collapse author list , Zhou L., Liu J., Yuan Q., Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A.
Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DC3000.
[5]"Genomewide identification of proteins secreted by the Hrp type III protein secretion system of Pseudomonas syringae pv. tomato DC3000."
Petnicki-Ocwieja T., Schneider D.J., Tam V.C., Chancey S.T., Shan L., Jamir Y., Schechter L.M., Janes M.D., Buell C.R., Tang X., Collmer A., Alfano J.R.
Proc. Natl. Acad. Sci. U.S.A. 99:7652-7657(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
Strain: DC3000.
[6]"A translocated protein tyrosine phosphatase of Pseudomonas syringae pv. tomato DC3000 modulates plant defence response to infection."
Bretz J.R., Mock N.M., Charity J.C., Zeyad S., Baker C.J., Hutcheson S.W.
Mol. Microbiol. 49:389-400(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A PHOSPHATASE, DOMAIN, INDUCTION.
Strain: DC3000.
[7]"The Arabidopsis PEPR pathway couples local and systemic plant immunity."
Ross A., Yamada K., Hiruma K., Yamashita-Yamada M., Lu X., Takano Y., Tsuda K., Saijo Y.
EMBO J. 33:62-75(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH EFR AND FLS2, MUTAGENESIS OF CYS-378.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF469470 Genomic DNA. Translation: AAO33450.1.
AY198373 Genomic DNA. Translation: AAO43976.1.
AE016853 Genomic DNA. Translation: AAO58160.1.
RefSeqNP_794465.1. NC_004578.1.

3D structure databases

ProteinModelPortalQ79LY0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING223283.PSPTO_4722.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO58160; AAO58160; PSPTO_4722.
GeneID1186405.
KEGGpst:PSPTO_4722.
PATRIC20000952. VBIPseSyr93040_4834.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGNOG14544.
OMARESAMIT.
OrthoDBEOG68WR39.

Enzyme and pathway databases

BioCycPSYR223283:GJIX-4787-MONOMER.

Family and domain databases

Gene3D3.90.190.10. 2 hits.
InterProIPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
SUPFAMSSF52799. SSF52799. 2 hits.
PROSITEPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHOPD2_PSESM
AccessionPrimary (citable) accession number: Q79LY0
Secondary accession number(s): Q87W44
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families