Q79LY0 (HOPD2_PSESM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Effector protein hopD2
EC=3.1.3.48

Alternative name(s):
Tyrosine-protein phosphatase hopPtoD2

Gene names

Name:	hopD2
Synonyms:	hopPtoD2
Ordered Locus Names:	PSPTO_4722

Organism

Pseudomonas syringae pv. tomato (strain DC3000) [Complete proteome] [HAMAP]

Taxonomic identifier

223283 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae group › Pseudomonas syringae group genomosp. 3 › Pseudomonas syringae pv. tomato

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Effector showing tyrosine-phosphatase activity required for host defense suppression. Functions inside plant cells causing suppression of HR (hypersensitive response), PR1 gene expression and oxidative burst probably by interfering with a MAPK (mitogen-activated protein kinase) pathway. MAPK cascades are known to activate defense-related transcription factors. Ref.3 Ref.6
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Enzyme regulation	Inhibited by sodium orthovanadate.
Subcellular location	Secreted. Note: Secreted via type III secretion system (TTSS). Ref.5
Induction	Transcriptionally induced by HrpL. Ref.3 Ref.6
Domain	The N-terminal domain (residues 1-142) retains 70% identity to the N-terminal 142 residues of AvrPphD, and is required for translocation into plant cells via TTSS. Ref.6
Miscellaneous	HopPtoD2 is not present in all strains of P.syringae.
Sequence similarities	Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Biological process	Hypersensitive response elicitation Virulence
Cellular component	Secreted
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	modulation by symbiont of host defense-related programmed cell death Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	Effector protein hopD2		PRO_0000094863
Regions
	Domain	143 – 468	326	Tyrosine-protein phosphatase
Sites
	Active site	378	1	Phosphocysteine intermediate
Experimental info
	Mutagenesis	378	1	C → S: Loss of phosphatase activity. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q79LY0 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 351D7B79664F9E81	FASTA	468	51,380
	10 20 30 40 50 60 MNPLQPIQHS ITNSQMSGGQ QLEAEGSQAH NSYSHPDRIS LSQLSQSAHL ALDHLSTQPN 70 80 90 100 110 120 TDHQRVASLV RNAVQDGKFQ LQSSNDTQVT YKTSVCPPAN ADTMGAAHLI NNELTVQARL 130 140 150 160 170 180 NDQLEYDIVS AHLYGPSEAI SIDASSPPSA NDLASSGLSE RTHLGMNRVL LRYAVPPRET 190 200 210 220 230 240 EDQCVMVIDK MPPPKHGKMS FFRTTNDLSK LPLGMETGGL SDLKLAGCER ISSVEQVKSI 250 260 270 280 290 300 RAALGGGPLT VLDLREESHA IVNGLPITLR GPMDWANAGL SQVDGAARES AMITELKRTK 310 320 330 340 350 360 SLTLVDANYV KGKKSNPQTT ELKNLNVRSE REVVTEAGAT YRRVAITDHN RPSPEATDEL 370 380 390 400 410 420 VDIMRHCLQA NESLVVHCNG GRGRTTTAMI MVDMLKNARN HSAETLITRM AKLSYDYNMT 430 440 450 460 DLGSISALKR PFLEDRLKFL QAFHDYARNN PSGLSLNWTQ WRAKIALE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A gene in the Pseudomonas syringae pv. tomato Hrp pathogenicity island conserved effector locus, hopPtoA1, contributes to efficient formation of bacterial colonies in planta and is duplicated elsewhere in the genome." Badel J.L., Charkowski A.O., Deng W.-L., Collmer A. Mol. Plant Microbe Interact. 15:1014-1024(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DC3000.
[2]	Buell C.R., Berry K.J., Fedorova N.B., Feldblynm T.V., Gwinn M.L., Haft D.H., Khouri H.M., Nelson W.C., Peterson J., Russell D., Tran B., Umayam L., Utterback T.R., Van Aken S.E. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DC3000.
[3]	"The Pseudomonas syringae type III-secreted protein HopPtoD2 possesses protein tyrosine phosphatase activity and suppresses programmed cell death in plants." Espinosa A., Guo M., Tam V.C., Fu Z.Q., Alfano J.R. Mol. Microbiol. 49:377-387(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A PHOSPHATASE, INDUCTION, MUTAGENESIS OF CYS-378. Strain: DC3000.
[4]	"The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000." Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T., Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C., Davidsen T.M., Zafar N. , Zhou L., Liu J., Yuan Q., Khouri H.M., Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R., Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R., Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G., Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M., Collmer A. Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DC3000.
[5]	"Genomewide identification of proteins secreted by the Hrp type III protein secretion system of Pseudomonas syringae pv. tomato DC3000." Petnicki-Ocwieja T., Schneider D.J., Tam V.C., Chancey S.T., Shan L., Jamir Y., Schechter L.M., Janes M.D., Buell C.R., Tang X., Collmer A., Alfano J.R. Proc. Natl. Acad. Sci. U.S.A. 99:7652-7657(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: DC3000.
[6]	"A translocated protein tyrosine phosphatase of Pseudomonas syringae pv. tomato DC3000 modulates plant defence response to infection." Bretz J.R., Mock N.M., Charity J.C., Zeyad S., Baker C.J., Hutcheson S.W. Mol. Microbiol. 49:389-400(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A PHOSPHATASE, DOMAIN, INDUCTION. Strain: DC3000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF469470 Genomic DNA. Translation: AAO33450.1. AY198373 Genomic DNA. Translation: AAO43976.1. AE016853 Genomic DNA. Translation: AAO58160.1.
RefSeq	NP_794465.1. NC_004578.1.
3D structure databases
ProteinModelPortal	Q79LY0.
ModBase	Search...
Protein-protein interaction databases
STRING	223283.PSPTO_4722.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO58160; AAO58160; PSPTO_4722.
GeneID	1186405.
KEGG	pst:PSPTO_4722.
PATRIC	20000952. VBIPseSyr93040_4834.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG14544.
ProtClustDB	CLSK538808.
Enzyme and pathway databases
BioCyc	PSYR223283:GJIX-4787-MONOMER.
Family and domain databases
InterPro	IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. IPR000242. Tyr_Pase_rcpt/non-rcpt. [Graphical view]
Pfam	PF00102. Y_phosphatase. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOPD2_PSESM

Accession

Primary (citable) accession number: Q79LY0
Secondary accession number(s): Q87W44

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program