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Protein

Effector protein hopD2

Gene

hopD2

Organism
Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Effector showing tyrosine-phosphatase activity required for host defense suppression. Functions inside plant cells causing suppression of HR (hypersensitive response), PR1 gene expression and oxidative burst probably by interfering with a MAPK (mitogen-activated protein kinase) pathway. MAPK cascades are known to activate defense-related transcription factors. Inhibits plant pattern-recognition receptors (PRRs) activation.3 Publications

Miscellaneous

HopPtoD2 is not present in all strains of P.syringae.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Enzyme regulationi

Inhibited by sodium orthovanadate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei378Phosphocysteine intermediate1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processHypersensitive response elicitation, Virulence

Enzyme and pathway databases

BioCyciPSYR223283:G1G0D-4788-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Effector protein hopD2 (EC:3.1.3.48)
Alternative name(s):
Tyrosine-protein phosphatase hopPtoD2
Gene namesi
Name:hopD2
Synonyms:hopA01, hopPtoD2
Ordered Locus Names:PSPTO_4722
OrganismiPseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002515 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi378C → S: Loss of phosphatase activity and decreased inhibition of EFR phosphorylation. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000948631 – 468Effector protein hopD2Add BLAST468

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ79LY0

Expressioni

Inductioni

Transcriptionally induced by HrpL.2 Publications

Interactioni

Subunit structurei

Interacts with EFR and FLS2 (via the kinase and cytoplasmic domains).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
EFRC0LGT63EBI-16096022,EBI-8801168From Arabidopsis thaliana.

Protein-protein interaction databases

DIPiDIP-61675N
IntActiQ79LY0, 2 interactors
STRINGi223283.PSPTO_4722

Structurei

3D structure databases

ProteinModelPortaliQ79LY0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini143 – 468Tyrosine-protein phosphataseAdd BLAST326

Domaini

The N-terminal domain (residues 1-142) retains 70% identity to the N-terminal 142 residues of AvrPphD, and is required for translocation into plant cells via TTSS.1 Publication

Phylogenomic databases

eggNOGiENOG41090FM Bacteria
ENOG4111Q7B LUCA
OrthoDBiPOG091H1BRB

Family and domain databases

Gene3Di3.90.190.10, 2 hits
InterProiView protein in InterPro
IPR029021 Prot-tyrosine_phosphatase-like
IPR016130 Tyr_Pase_AS
IPR000387 TYR_PHOSPHATASE_dom
SUPFAMiSSF52799 SSF52799, 2 hits
PROSITEiView protein in PROSITE
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q79LY0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPLQPIQHS ITNSQMSGGQ QLEAEGSQAH NSYSHPDRIS LSQLSQSAHL
60 70 80 90 100
ALDHLSTQPN TDHQRVASLV RNAVQDGKFQ LQSSNDTQVT YKTSVCPPAN
110 120 130 140 150
ADTMGAAHLI NNELTVQARL NDQLEYDIVS AHLYGPSEAI SIDASSPPSA
160 170 180 190 200
NDLASSGLSE RTHLGMNRVL LRYAVPPRET EDQCVMVIDK MPPPKHGKMS
210 220 230 240 250
FFRTTNDLSK LPLGMETGGL SDLKLAGCER ISSVEQVKSI RAALGGGPLT
260 270 280 290 300
VLDLREESHA IVNGLPITLR GPMDWANAGL SQVDGAARES AMITELKRTK
310 320 330 340 350
SLTLVDANYV KGKKSNPQTT ELKNLNVRSE REVVTEAGAT YRRVAITDHN
360 370 380 390 400
RPSPEATDEL VDIMRHCLQA NESLVVHCNG GRGRTTTAMI MVDMLKNARN
410 420 430 440 450
HSAETLITRM AKLSYDYNMT DLGSISALKR PFLEDRLKFL QAFHDYARNN
460
PSGLSLNWTQ WRAKIALE
Length:468
Mass (Da):51,380
Last modified:July 5, 2004 - v1
Checksum:i351D7B79664F9E81
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF469470 Genomic DNA Translation: AAO33450.1
AY198373 Genomic DNA Translation: AAO43976.1
AE016853 Genomic DNA Translation: AAO58160.1
RefSeqiNP_794465.1, NC_004578.1
WP_011105134.1, NC_004578.1

Genome annotation databases

EnsemblBacteriaiAAO58160; AAO58160; PSPTO_4722
GeneIDi1186405
KEGGipst:PSPTO_4722
PATRICifig|223283.9.peg.4834

Similar proteinsi

Entry informationi

Entry nameiHOPD2_PSESM
AccessioniPrimary (citable) accession number: Q79LY0
Secondary accession number(s): Q87W44
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health