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Q79LY0

- HOPD2_PSESM

UniProt

Q79LY0 - HOPD2_PSESM

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Protein
Effector protein hopD2
Gene
hopD2, hopA01, hopPtoD2, PSPTO_4722
Organism
Pseudomonas syringae pv. tomato (strain DC3000)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Effector showing tyrosine-phosphatase activity required for host defense suppression. Functions inside plant cells causing suppression of HR (hypersensitive response), PR1 gene expression and oxidative burst probably by interfering with a MAPK (mitogen-activated protein kinase) pathway. MAPK cascades are known to activate defense-related transcription factors. Inhibits plant pattern-recognition receptors (PRRs) activation.3 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Enzyme regulationi

Inhibited by sodium orthovanadate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei378 – 3781Phosphocysteine intermediate

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. modulation by symbiont of host defense-related programmed cell death Source: UniProtKB-KW
  2. pathogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Hypersensitive response elicitation, Virulence

Enzyme and pathway databases

BioCyciPSYR223283:GJIX-4787-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Effector protein hopD2 (EC:3.1.3.48)
Alternative name(s):
Tyrosine-protein phosphatase hopPtoD2
Gene namesi
Name:hopD2
Synonyms:hopA01, hopPtoD2
Ordered Locus Names:PSPTO_4722
OrganismiPseudomonas syringae pv. tomato (strain DC3000)
Taxonomic identifieri223283 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002515: Chromosome

Subcellular locationi

Secreted
Note: Secreted via type III secretion system (TTSS).1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi378 – 3781C → S: Loss of phosphatase activity and decreased inhibition of EFR phosphorylation. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 468468Effector protein hopD2
PRO_0000094863Add
BLAST

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

Transcriptionally induced by HrpL.2 Publications

Interactioni

Subunit structurei

Interacts with EFR and FLS2 (via the kinase and cytoplasmic domains).1 Publication

Protein-protein interaction databases

STRINGi223283.PSPTO_4722.

Structurei

3D structure databases

ProteinModelPortaliQ79LY0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini143 – 468326Tyrosine-protein phosphatase
Add
BLAST

Domaini

The N-terminal domain (residues 1-142) retains 70% identity to the N-terminal 142 residues of AvrPphD, and is required for translocation into plant cells via TTSS.1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG14544.
OMAiRESAMIT.
OrthoDBiEOG68WR39.

Family and domain databases

Gene3Di3.90.190.10. 2 hits.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 2 hits.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q79LY0-1 [UniParc]FASTAAdd to Basket

« Hide

MNPLQPIQHS ITNSQMSGGQ QLEAEGSQAH NSYSHPDRIS LSQLSQSAHL    50
ALDHLSTQPN TDHQRVASLV RNAVQDGKFQ LQSSNDTQVT YKTSVCPPAN 100
ADTMGAAHLI NNELTVQARL NDQLEYDIVS AHLYGPSEAI SIDASSPPSA 150
NDLASSGLSE RTHLGMNRVL LRYAVPPRET EDQCVMVIDK MPPPKHGKMS 200
FFRTTNDLSK LPLGMETGGL SDLKLAGCER ISSVEQVKSI RAALGGGPLT 250
VLDLREESHA IVNGLPITLR GPMDWANAGL SQVDGAARES AMITELKRTK 300
SLTLVDANYV KGKKSNPQTT ELKNLNVRSE REVVTEAGAT YRRVAITDHN 350
RPSPEATDEL VDIMRHCLQA NESLVVHCNG GRGRTTTAMI MVDMLKNARN 400
HSAETLITRM AKLSYDYNMT DLGSISALKR PFLEDRLKFL QAFHDYARNN 450
PSGLSLNWTQ WRAKIALE 468
Length:468
Mass (Da):51,380
Last modified:July 5, 2004 - v1
Checksum:i351D7B79664F9E81
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF469470 Genomic DNA. Translation: AAO33450.1.
AY198373 Genomic DNA. Translation: AAO43976.1.
AE016853 Genomic DNA. Translation: AAO58160.1.
RefSeqiNP_794465.1. NC_004578.1.

Genome annotation databases

EnsemblBacteriaiAAO58160; AAO58160; PSPTO_4722.
GeneIDi1186405.
KEGGipst:PSPTO_4722.
PATRICi20000952. VBIPseSyr93040_4834.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF469470 Genomic DNA. Translation: AAO33450.1 .
AY198373 Genomic DNA. Translation: AAO43976.1 .
AE016853 Genomic DNA. Translation: AAO58160.1 .
RefSeqi NP_794465.1. NC_004578.1.

3D structure databases

ProteinModelPortali Q79LY0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 223283.PSPTO_4722.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO58160 ; AAO58160 ; PSPTO_4722 .
GeneIDi 1186405.
KEGGi pst:PSPTO_4722.
PATRICi 20000952. VBIPseSyr93040_4834.

Phylogenomic databases

eggNOGi NOG14544.
OMAi RESAMIT.
OrthoDBi EOG68WR39.

Enzyme and pathway databases

BioCyci PSYR223283:GJIX-4787-MONOMER.

Family and domain databases

Gene3Di 3.90.190.10. 2 hits.
InterProi IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view ]
SUPFAMi SSF52799. SSF52799. 2 hits.
PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A gene in the Pseudomonas syringae pv. tomato Hrp pathogenicity island conserved effector locus, hopPtoA1, contributes to efficient formation of bacterial colonies in planta and is duplicated elsewhere in the genome."
    Badel J.L., Charkowski A.O., Deng W.-L., Collmer A.
    Mol. Plant Microbe Interact. 15:1014-1024(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DC3000.
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DC3000.
  3. "The Pseudomonas syringae type III-secreted protein HopPtoD2 possesses protein tyrosine phosphatase activity and suppresses programmed cell death in plants."
    Espinosa A., Guo M., Tam V.C., Fu Z.Q., Alfano J.R.
    Mol. Microbiol. 49:377-387(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A PHOSPHATASE, INDUCTION, MUTAGENESIS OF CYS-378.
    Strain: DC3000.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DC3000.
  5. "Genomewide identification of proteins secreted by the Hrp type III protein secretion system of Pseudomonas syringae pv. tomato DC3000."
    Petnicki-Ocwieja T., Schneider D.J., Tam V.C., Chancey S.T., Shan L., Jamir Y., Schechter L.M., Janes M.D., Buell C.R., Tang X., Collmer A., Alfano J.R.
    Proc. Natl. Acad. Sci. U.S.A. 99:7652-7657(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: DC3000.
  6. "A translocated protein tyrosine phosphatase of Pseudomonas syringae pv. tomato DC3000 modulates plant defence response to infection."
    Bretz J.R., Mock N.M., Charity J.C., Zeyad S., Baker C.J., Hutcheson S.W.
    Mol. Microbiol. 49:389-400(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A PHOSPHATASE, DOMAIN, INDUCTION.
    Strain: DC3000.
  7. "The Arabidopsis PEPR pathway couples local and systemic plant immunity."
    Ross A., Yamada K., Hiruma K., Yamashita-Yamada M., Lu X., Takano Y., Tsuda K., Saijo Y.
    EMBO J. 33:62-75(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH EFR AND FLS2, MUTAGENESIS OF CYS-378.

Entry informationi

Entry nameiHOPD2_PSESM
AccessioniPrimary (citable) accession number: Q79LY0
Secondary accession number(s): Q87W44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

HopPtoD2 is not present in all strains of P.syringae.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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