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Q79FX6

- MMAA2_MYCTU

UniProt

Q79FX6 - MMAA2_MYCTU

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Protein

Cyclopropane mycolic acid synthase MmaA2

Gene

mmaA2

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of a double bond to a cis cyclopropane ring at the distal position of an alpha mycolic acid via the transfer of a methylene group from S-adenosyl-L-methionine. MmaA2 also catalyzes the biosynthesis of the cis-cyclopropanated methoxymycolates. Cyclopropanated mycolic acids are key factors participating in cell envelope permeability, host immunomodulation and persistence.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid.

Enzyme regulationi

Inhibited by S-adenosyl-N-decyl-aminoethyl (SADAE) and thiacetazone (TAC).2 Publications

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei136 – 1361S-adenosyl-L-methionine; via carbonyl oxygen
Active sitei269 – 2691By similarity

GO - Molecular functioni

  1. cyclopropane-fatty-acyl-phospholipid synthase activity Source: UniProtKB
  2. methyltransferase activity Source: UniProtKB

GO - Biological processi

  1. mycolic acid biosynthetic process Source: MTBBASE
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:RV0644C-MONOMER.
MTBRV:RV0644C-MONOMER.
UniPathwayiUPA00915.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclopropane mycolic acid synthase MmaA2 (EC:2.1.1.79)
Short name:
CMAS
Alternative name(s):
Cyclopropane-fatty-acyl-phospholipid synthase
Short name:
CFA synthase
Mycolic acid methyltransferase
Short name:
MA-MT
S-adenosylmethionine-dependent methyltransferase
Short name:
AdoMet-MT
Short name:
SAM-MT
Gene namesi
Name:mmaA2
Synonyms:mma2
Ordered Locus Names:Rv0644c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001584: Chromosome

Organism-specific databases

TubercuListiRv0644c.

Subcellular locationi

GO - Cellular componenti

  1. cell wall Source: MTBBASE
  2. plasma membrane Source: MTBBASE
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Disruption of this gene suppresses a cyclopropane group at the distal position of alpha mycolic acid and cause a mild impairment in methoxymycolate, but not in ketomycolate. A 2-fold reduction in the relative abundance of cis-cyclopropanated methoxymycolate is observed. Cells lacking both cmA2 and mmaA2 genes cannot cis cyclopropanate methoxymycolates or ketomycolates.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 287286Cyclopropane mycolic acid synthase MmaA2PRO_0000398359Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylvaline1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ79FX6.

Interactioni

Protein-protein interaction databases

STRINGi83332.Rv0644c.

Structurei

Secondary structure

1
287
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 168
Helixi20 – 245
Helixi45 – 5713
Turni58 – 614
Beta strandi67 – 726
Helixi77 – 8610
Beta strandi89 – 957
Helixi97 – 10812
Beta strandi116 – 1216
Helixi123 – 1253
Beta strandi131 – 1377
Helixi139 – 1424
Helixi144 – 1463
Helixi147 – 15711
Beta strandi163 – 1719
Helixi174 – 1796
Helixi186 – 19813
Helixi208 – 21811
Beta strandi221 – 2277
Helixi229 – 24517
Helixi247 – 2537
Helixi256 – 27520
Beta strandi277 – 28610

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TPYX-ray2.20A1-287[»]
ProteinModelPortaliQ79FX6.
SMRiQ79FX6. Positions 3-287.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ79FX6.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni33 – 342S-adenosyl-L-methionine binding
Regioni72 – 743S-adenosyl-L-methionine binding
Regioni94 – 996S-adenosyl-L-methionine binding
Regioni123 – 1242S-adenosyl-L-methionine binding

Sequence similaritiesi

Belongs to the CFA/CMAS family.Curated

Phylogenomic databases

eggNOGiCOG2230.
HOGENOMiHOG000245191.
InParanoidiQ79FX6.
KOiK00574.
OMAiKDRQYSC.
OrthoDBiEOG6BGNZP.
PhylomeDBiQ79FX6.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003333. Mycolic_cyclopropane_synthase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02353. CMAS. 1 hit.
[Graphical view]
PIRSFiPIRSF003085. CMAS. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q79FX6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVNDLTPHFE DVQAHYDLSD DFFRLFLDPT QTYSCAHFER EDMTLEEAQI
60 70 80 90 100
AKIDLALGKL GLQPGMTLLD IGCGWGATMR RAIAQYDVNV VGLTLSKNQA
110 120 130 140 150
AHVQKSFDEM DTPRDRRVLL AGWEQFNEPV DRIVSIGAFE HFGHDRHADF
160 170 180 190 200
FARAHKILPP DGVLLLHTIT GLTRQQMVDH GLPLTLWLAR FLKFIATEIF
210 220 230 240 250
PGGQPPTIEM VEEQSAKTGF TLTRRQSLQP HYARTLDLWA EALQEHKSEA
260 270 280
IAIQSEEVYE RYMKYLTGCA KLFRVGYIDV NQFTLAK
Length:287
Mass (Da):32,724
Last modified:July 5, 2004 - v1
Checksum:i669C3224C6B178C0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL123456 Genomic DNA. Translation: CCP43387.1.
RefSeqiNP_215158.1. NC_000962.3.
YP_006513985.1. NC_018143.2.

Genome annotation databases

EnsemblBacteriaiCCP43387; CCP43387; Rv0644c.
GeneIDi13318530.
888061.
KEGGimtu:Rv0644c.
mtv:RVBD_0644c.
PATRICi18149942. VBIMycTub87468_0719.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL123456 Genomic DNA. Translation: CCP43387.1 .
RefSeqi NP_215158.1. NC_000962.3.
YP_006513985.1. NC_018143.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1TPY X-ray 2.20 A 1-287 [» ]
ProteinModelPortali Q79FX6.
SMRi Q79FX6. Positions 3-287.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 83332.Rv0644c.

Proteomic databases

PRIDEi Q79FX6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CCP43387 ; CCP43387 ; Rv0644c .
GeneIDi 13318530.
888061.
KEGGi mtu:Rv0644c.
mtv:RVBD_0644c.
PATRICi 18149942. VBIMycTub87468_0719.

Organism-specific databases

TubercuListi Rv0644c.

Phylogenomic databases

eggNOGi COG2230.
HOGENOMi HOG000245191.
InParanoidi Q79FX6.
KOi K00574.
OMAi KDRQYSC.
OrthoDBi EOG6BGNZP.
PhylomeDBi Q79FX6.

Enzyme and pathway databases

UniPathwayi UPA00915 .
BioCyci MetaCyc:RV0644C-MONOMER.
MTBRV:RV0644C-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q79FX6.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR003333. Mycolic_cyclopropane_synthase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02353. CMAS. 1 hit.
[Graphical view ]
PIRSFi PIRSF003085. CMAS. 1 hit.
SUPFAMi SSF53335. SSF53335. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  2. "The mmaA2 gene of Mycobacterium tuberculosis encodes the distal cyclopropane synthase of the alpha-mycolic acid."
    Glickman M.S.
    J. Biol. Chem. 278:7844-7849(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN THE BIOSYNTHESIS OF CYCLOPROPANE RING IN THE ALPHA MYCOLATE, DISRUPTION PHENOTYPE.
    Strain: ATCC 35801 / TMC 107 / Erdman.
  3. "Thiacetazone, an antitubercular drug that inhibits cyclopropanation of cell wall mycolic acids in mycobacteria."
    Alahari A., Trivelli X., Guerardel Y., Dover L.G., Besra G.S., Sacchettini J.C., Reynolds R.C., Coxon G.D., Kremer L.
    PLoS ONE 2:E1343-E1343(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
    Strain: ATCC 25618 / H37Rv.
  4. "targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis."
    Raman K., Yeturu K., Chandra N.
    BMC Syst. Biol. 2:109-109(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
  5. "S-adenosyl-N-decyl-aminoethyl, a potent bisubstrate inhibitor of mycobacterium tuberculosis mycolic acid methyltransferases."
    Vaubourgeix J., Bardou F., Boissier F., Julien S., Constant P., Ploux O., Daffe M., Quemard A., Mourey L.
    J. Biol. Chem. 284:19321-19330(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
    Strain: ATCC 25618 / H37Rv.
  6. "Redundant function of cmaA2 and mmaA2 in Mycobacterium tuberculosis cis cyclopropanation of oxygenated mycolates."
    Barkan D., Rao V., Sukenick G.D., Glickman M.S.
    J. Bacteriol. 192:3661-3668(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN OXYGEN-CONTAINING MYCOLATES AND IN ALPHA-MYCOLATES BIOSYNTHESIS.
  7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT VAL-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.
  8. "Structure of the cyclopropane synthase mmaA2 from Mycobacterium tuberculosis."
    Smith C.V., Sacchettini J.C.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH S-ADENOSYLMETHIONINE.
    Strain: ATCC 25618 / H37Rv.

Entry informationi

Entry nameiMMAA2_MYCTU
AccessioniPrimary (citable) accession number: Q79FX6
Secondary accession number(s): L0T7B6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 5, 2004
Last modified: October 29, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3