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Q79EC5

- PARE_ECOLX

UniProt

Q79EC5 - PARE_ECOLX

Protein

Toxin ParE

Gene

parE

Organism
Escherichia coli
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Toxin component of a toxin-antitoxin (TA) module involved in plasmid partition. Acts by inhibiting DNA gyrase, converting supercoiled plasmid DNA into a singly cleaved linear form in an ATP-dependent fashion. To do so it probably interacts with one of the gyrase subunits. Gyrase inhibition is prevented by antitoxin ParD, and is reversed in vitro by incubation with ParD. In cells that have lost the plasmid filamentation and growth inhibition occur; this is suppressed when ParD is supplied in trans. The parDE operon alone is capable of stabilizing an RK2-derived minireplicon under defined growth conditions in several different Gram-negative bacteria. It does so by the post-segregational killing (PSK) of plasmid-free cells, also referred to as a plasmid addiction system.4 Publications

    GO - Molecular functioni

    1. DNA topoisomerase (ATP-hydrolyzing) inhibitor activity Source: UniProtKB
    2. protein binding Source: UniProtKB
    3. protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    1. negative regulation of DNA topoisomerase (ATP-hydrolyzing) activity Source: GOC
    2. plasmid partitioning Source: UniProtKB
    3. protein heterotetramerization Source: UniProtKB

    Keywords - Molecular functioni

    Toxin

    Keywords - Biological processi

    Plasmid partition

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Toxin ParE
    Alternative name(s):
    Gyrase inhibitor ParE
    Gene namesi
    Name:parE
    Encoded oniPlasmid IncP-alpha RP41 Publication
    Plasmid IncP-alpha RK23 Publications
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    Pathology & Biotechi

    Disruption phenotypei

    Loss of this gene blocks plasmid stabilizing ability.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 103103Toxin ParEPRO_0000408371Add
    BLAST

    Interactioni

    Subunit structurei

    Probably forms a homodimer in solution, interacts with ParD, possibly as a ParD(2)-ParE2 heterotetramer, which neutralizes the toxic activity of ParE. The heterotetramer binds DNA, but not ParE alone.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ79EC5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RelE toxin family.Curated

    Family and domain databases

    InterProiIPR007712. RelE/ParE_toxin.
    [Graphical view]
    PfamiPF05016. Plasmid_stabil. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q79EC5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAYILTAEA EADLRGIIRY TRREWGAAQV RRYIAKLEQG IARLAAGEGP    50
    FKDMSELFPA LRMARCEHHY VFCLPRAGEP ALVVAILHER MDLMTRLADR 100
    LKG 103
    Length:103
    Mass (Da):11,716
    Last modified:July 5, 2004 - v1
    Checksum:iDCDC13A6056E7132
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L05507 Genomic DNA. Translation: AAA92775.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L05507 Genomic DNA. Translation: AAA92775.1 .

    3D structure databases

    ProteinModelPortali Q79EC5.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR007712. RelE/ParE_toxin.
    [Graphical view ]
    Pfami PF05016. Plasmid_stabil. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Partitioning of broad-host-range plasmid RP4 is a complex system involving site-specific recombination."
      Gerlitz M., Hrabak O., Schwab H.
      J. Bacteriol. 172:6194-6203(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: IncP-alpha RP4
    2. "Definition of a minimal plasmid stabilization system from the broad-host-range plasmid RK2."
      Roberts R.C., Helinski D.R.
      J. Bacteriol. 174:8119-8132(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN PLASMID PARTITIONING, DISRUPTION PHENOTYPE.
      Plasmid: IncP-alpha RK2
    3. "The parDE operon of the broad-host-range plasmid RK2 specifies growth inhibition associated with plasmid loss."
      Roberts R.C., Strom A.R., Helinski D.R.
      J. Mol. Biol. 237:35-51(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A TOXIN.
    4. "Plasmid RK2 toxin protein ParE: purification and interaction with the ParD antitoxin protein."
      Johnson E.P., Strom A.R., Helinski D.R.
      J. Bacteriol. 178:1420-1429(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A TOXIN, SUBUNIT, INTERACTION WITH PARD.
      Plasmid: IncP-alpha RK2
    5. "ParE toxin encoded by the broad-host-range plasmid RK2 is an inhibitor of Escherichia coli gyrase."
      Jiang Y., Pogliano J., Helinski D.R., Konieczny I.
      Mol. Microbiol. 44:971-979(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A DNA GYRASE INHIBITOR.
      Plasmid: IncP-alpha RK2

    Entry informationi

    Entry nameiPARE_ECOLX
    AccessioniPrimary (citable) accession number: Q79EC5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 22 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Plasmid IncP-alpha RK2 is maintained at 5-8 copies per chromosome.

    Keywords - Technical termi

    Plasmid

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3