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Q79EC5 (PARE_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Toxin ParE
Alternative name(s):
Gyrase inhibitor ParE
Gene names
Name:parE
Encoded onPlasmid IncP-alpha RP4 Ref.1
Plasmid IncP-alpha RK2 Ref.2 Ref.4 Ref.5
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length103 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Toxin component of a toxin-antitoxin (TA) module involved in plasmid partition. Acts by inhibiting DNA gyrase, converting supercoiled plasmid DNA into a singly cleaved linear form in an ATP-dependent fashion. To do so it probably interacts with one of the gyrase subunits. Gyrase inhibition is prevented by antitoxin ParD, and is reversed in vitro by incubation with ParD. In cells that have lost the plasmid filamentation and growth inhibition occur; this is suppressed when ParD is supplied in trans. The parDE operon alone is capable of stabilizing an RK2-derived minireplicon under defined growth conditions in several different Gram-negative bacteria. It does so by the post-segregational killing (PSK) of plasmid-free cells, also referred to as a plasmid addiction system. Ref.2 Ref.3 Ref.4 Ref.5

Subunit structure

Probably forms a homodimer in solution, interacts with ParD, possibly as a ParD(2)-ParE2 heterotetramer, which neutralizes the toxic activity of ParE. The heterotetramer binds DNA, but not ParE alone. Ref.4

Disruption phenotype

Loss of this gene blocks plasmid stabilizing ability. Ref.2

Miscellaneous

Plasmid IncP-alpha RK2 is maintained at 5-8 copies per chromosome.

Sequence similarities

Belongs to the RelE toxin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 103103Toxin ParE
PRO_0000408371

Sequences

Sequence LengthMass (Da)Tools
Q79EC5 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: DCDC13A6056E7132

FASTA10311,716
        10         20         30         40         50         60 
MTAYILTAEA EADLRGIIRY TRREWGAAQV RRYIAKLEQG IARLAAGEGP FKDMSELFPA 

        70         80         90        100 
LRMARCEHHY VFCLPRAGEP ALVVAILHER MDLMTRLADR LKG 

« Hide

References

[1]"Partitioning of broad-host-range plasmid RP4 is a complex system involving site-specific recombination."
Gerlitz M., Hrabak O., Schwab H.
J. Bacteriol. 172:6194-6203(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Definition of a minimal plasmid stabilization system from the broad-host-range plasmid RK2."
Roberts R.C., Helinski D.R.
J. Bacteriol. 174:8119-8132(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN PLASMID PARTITIONING, DISRUPTION PHENOTYPE.
[3]"The parDE operon of the broad-host-range plasmid RK2 specifies growth inhibition associated with plasmid loss."
Roberts R.C., Strom A.R., Helinski D.R.
J. Mol. Biol. 237:35-51(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A TOXIN.
[4]"Plasmid RK2 toxin protein ParE: purification and interaction with the ParD antitoxin protein."
Johnson E.P., Strom A.R., Helinski D.R.
J. Bacteriol. 178:1420-1429(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A TOXIN, SUBUNIT, INTERACTION WITH PARD.
[5]"ParE toxin encoded by the broad-host-range plasmid RK2 is an inhibitor of Escherichia coli gyrase."
Jiang Y., Pogliano J., Helinski D.R., Konieczny I.
Mol. Microbiol. 44:971-979(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A DNA GYRASE INHIBITOR.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L05507 Genomic DNA. Translation: AAA92775.1.

3D structure databases

ProteinModelPortalQ79EC5.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR007712. RelE/ParE_toxin.
[Graphical view]
PfamPF05016. Plasmid_stabil. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePARE_ECOLX
AccessionPrimary (citable) accession number: Q79EC5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families