Reviewed, UniProtKB/Swiss-Prot Q796V8 (HEMZ_BACSU)

Names and origin

Protein names

Recommended name:
    Oxygen-independent coproporphyrinogen III oxidase 2
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.99.22

Gene names

Name:	hemZ
Synonyms:	yhaV, yhaW
Ordered Locus Names:	BSU09840

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX.
Catalytic activity	Coproporphyrinogen-III + 2 S-adenosyl-L-methionine = protoporphyrinogen-IX + 2 CO(2) + 2 L-methionine + 2 5'-deoxyadenosine.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen III (AdoMet route): step 1/1.
Induction	Induced, in anaerobic conditions, by resDE, fnr and arfM.
Sequence similarities	Belongs to the anaerobic coproporphyrinogen III oxidase family.
Sequence caution	The sequence CAA74441.1 differs from that shown. Reason: Frameshift at position 160. The sequence CAA74454.1 differs from that shown. Reason: Frameshift at position 160.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW porphyrin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW coproporphyrinogen dehydrogenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Oxygen-independent coproporphyrinogen III oxidase 2		PRO_0000109955
Regions
	Region	234 – 235	2	S-adenosyl-L-methionine 2 binding By similarity
Sites
	Metal binding	180	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	184	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	187	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Binding site	174	1	S-adenosyl-L-methionine 1 By similarity
	Binding site	186	1	S-adenosyl-L-methionine 2; via carbonyl oxygen By similarity
	Binding site	233	1	S-adenosyl-L-methionine 1; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	267	1	S-adenosyl-L-methionine 1 By similarity
	Binding site	295	1	S-adenosyl-L-methionine 2 By similarity
	Binding site	307	1	S-adenosyl-L-methionine 2 By similarity
	Binding site	332	1	S-adenosyl-L-methionine 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q796V8-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 8E4C2373D1D9190D	FASTA	501	57,527
	10 20 30 40 50 60 MQIKIEGIHD DRLHRPLQNI ANLFYEECEL AYGGEEPADF VISLALSQTD EHVTVSGEVK 70 80 90 100 110 120 GTGIKEQHTK FFSPDMTEKE AFKQVKNTIS YVYLNLLQAH TGITQKWGIL TGIRPTKLLH 130 140 150 160 170 180 KKLQSGMSKE QAHAELKKDY LIHDEKIMLM QEIVDRQLAA VPDLYRVKDE VSIYIGIPFC 190 200 210 220 230 240 PTKCAYCTFP AYAIQGQAGR VGSFLWGLHY EMQKIGEWLK EHDVKVTTIY FGGGTPTSIT 250 260 270 280 290 300 AEEMDLLYEE MVRSFPDVKN IREITVEAGR PDTITEEKLA VLNKYDIDRI SINPQSYENE 310 320 330 340 350 360 TLKAIGRHHT VEETIEKYHL SRQHGMNNIN MDLIIGLPGE GVKEFRHSLS ETEKLMPESL 370 380 390 400 410 420 TVHTLSFKRA SEMTRNKHKY KVAGREEVSQ MMEDAVAWTK EHGYVPYYLY RQKNILGNLE 430 440 450 460 470 480 NVGYSLPGQE SIYNIMIMEE VQTIIGIGCG AASKFIDRDT GKITHFANPK DPKSYNERFE 490 500 HYTDEKIKYL EQIFEKTTKQ H

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus subtilis chromosome contains several dysfunctional genes, the glyB marker, many genes encoding transporter proteins, and the ubiquitous hit gene." Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H., Venema G., Bron S. Microbiology 144:859-875(1998) [PubMed: 9579061] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"Detecting and analyzing DNA sequencing errors: toward a higher quality of the Bacillus subtilis genome sequence." Medigue C., Rose M., Viari A., Danchin A. Genome Res. 9:1116-1127(1999) [PubMed: 10568751] [Abstract] Cited for: SEQUENCE REVISION.
[4]	"Transcriptional control of Bacillus subtilis hemN and hemZ." Homuth G., Rompf A., Schumann W., Jahn D. J. Bacteriol. 181:5922-5929(1999) [PubMed: 10498703] [Abstract] Cited for: CHARACTERIZATION, REGULATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y14080 Genomic DNA. Translation: CAA74454.1. Frameshift. Y14080 Genomic DNA. Translation: CAA74441.1. Frameshift. Z99109 Genomic DNA. Translation: CAB12823.2.
PIR	A69820. H69819.
RefSeq	NP_388865.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	937935.
GenomeReviews	Gene locus BSU09840 in contig AL009126_GR.
KEGG	bsu:BSU09840.
NMPDR	fig|224308.1.peg.984.
Organism-specific databases
SubtiList	BG12999. hemZ. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	Q796V8.
Enzyme and pathway databases
BioCyc	BSUB224308:YHAW-MON.
Family and domain databases
InterPro	IPR006638. Elp3/MiaB/NifB. IPR007197. Radical_SAM. [Graphical view]
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMZ_BACSU

Accession

Primary (citable) accession number: Q796V8
Secondary accession number(s): O07537, O07538

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	July 5, 2004
Last modified:	November 4, 2008
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)