Reviewed, UniProtKB/Swiss-Prot Q795Y4 (FDHL_BACSU)

Names and origin

Protein names

Recommended name:
    Putative formate dehydrogenase yrhE
    EC=1.2.1.2

Gene names

Name:	yrhE
Ordered Locus Names:	BSU27220

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	980 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Formate + NAD+ = CO2 + NADH.
Cofactor	Binds 1 2Fe-2S cluster By similarity. Binds 4 4Fe-4S clusters By similarity. Molybdenum (molybdopterin) By similarity.
Sequence similarities	In the C-terminal section; belongs to the prokaryotic molybdopterin-containing oxidoreductase family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Domain	Repeat
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	formate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	formate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro formate dehydrogenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 980	980	Putative formate dehydrogenase yrhE		PRO_0000360689
Regions
	Domain	5 – 81	77	2Fe-2S ferredoxin-type
	Domain	144 – 171	28	4Fe-4S ferredoxin-type 1
	Domain	187 – 216	30	4Fe-4S ferredoxin-type 2
	Region	258 – 980	723	Formate dehydrogenase By similarity
Sites
	Metal binding	39	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	50	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	53	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	65	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	97	1	Iron-sulfur 2 (4Fe-4S); via tele nitrogen By similarity
	Metal binding	101	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	104	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	111	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	153	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	156	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	159	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	163	1	Iron-sulfur 4 (4Fe-4S) By similarity
	Metal binding	196	1	Iron-sulfur 4 (4Fe-4S) By similarity
	Metal binding	199	1	Iron-sulfur 4 (4Fe-4S) By similarity
	Metal binding	202	1	Iron-sulfur 4 (4Fe-4S) By similarity
	Metal binding	206	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	270	1	Iron-sulfur 5 (4Fe-4S) By similarity
	Metal binding	273	1	Iron-sulfur 5 (4Fe-4S) By similarity
	Metal binding	277	1	Iron-sulfur 5 (4Fe-4S) By similarity
	Metal binding	305	1	Iron-sulfur 5 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q795Y4-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 0DA2B17A506806ED	FASTA	980	108,775
	10 20 30 40 50 60 MMDVKSISVR VDGTEIQARA GATILDILNE NGIEYPQICH VPEVDPIQTC DTCIVEANGK 70 80 90 100 110 120 LVRSCATVAE NGMSIDLSGN RVKEAQTEAM DRLLENHLLY CTVCDNNNGN CTLHNTAEMM 130 140 150 160 170 180 GIEHQKYPYT PKEDPSCAVD MSHPFYRYDP NQCIACGQCV EVCQNLQVNE TLSIDWERER 190 200 210 220 230 240 PRVIWDEGVP INESSCVSCG QCVTVCPCNA LMEKSMLGQA GFMTGIKEDV MEPMIDLVKN 250 260 270 280 290 300 VEPGYGSIFA ISEVEAAMRE TRTKKTKTVC TFCGVGCSFE VWTKGRDILK IQPVSDAPVN 310 320 330 340 350 360 AISTCVKGKF GWDFVNSKER ITKPLIRKNG AFVESSWEEA LDLVASRLGS IKEQYGKGSV 370 380 390 400 410 420 GFISSSKITN EENYVIQKLA RQVFETNNVD NCSRYCQSPA TDGLFRTVGM GGDAGTIKDI 430 440 450 460 470 480 AKAGLVIIVG ANPAEGHPVL ATRVKRAHKL HGQKLIVADL RKNEMAERSD LFISPKQGTD 490 500 510 520 530 540 QVWLMAVTKY MIDQGWHDQA FIDENVNYFE DYKETLKTYT LDYAERITGL SKENIIQIAE 550 560 570 580 590 600 MIRDADGTCV LWGMGVTQNT GGSDTSAAIS NLLLATGNYR RPGAGAYPLR GHNNVQGACD 610 620 630 640 650 660 MGTLPGWLPG YQHITDDKAR AKFEEAYGVE IDGKPGLDNI QMLHAIEEGK MKAMYLVGED 670 680 690 700 710 720 MALVDSNANH VHDILSSLDF FVVQDIFLSR TAQYADVVLP ATPSLEKDGT FTNTERRVQR 730 740 750 760 770 780 LYQALPTLGD AKPDWWIIQE VANRLGANWN YSHPSDIFSE MASLSPLFAK ASYEVLEGWN 790 800 810 820 830 840 SFLWGSFTGE STPLLYEDGF NFPDKKARFA LSDWTEPAAF PEEYDLHINN GRMLEHFHEG 850 860 870 880 890 900 NMTNKSKGIQ AKVPNVFVEI SPELAQERGV CDGSLVRLVS PFGAVKLNAL ITDRVRKNEL 910 920 930 940 950 960 YLPMNSTDKE SAINFLTGPA ADKRTNTPAY KQTKVRMEVL GGCETAPLPK TNPRNKKRHP 970 980 QNGVEAERKW NRPGYVHLTD

References

[1]

"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL009126 Genomic DNA. Translation: CAB14664.1.
RefSeq	NP_390600.1.
3D structure databases
HSSP	HSSP built from PDB template 1AA6 based on UniProtKB P07658.
ModBase	Search...
Genome annotation databases
GeneID	937569.
GenomeReviews	Gene locus BSU27220 in contig AL009126_GR.
KEGG	bsu:BSU27220.
NMPDR	fig|224308.1.peg.2725.
Organism-specific databases
SubtiList	BG12294. yrhE. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	Q795Y4.
OMA	FETNNID.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU2718-MON.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR001450. 4Fe4S_Fe_S_bd_subgr. IPR017900. 4Fe4S_Fe_S_CS. IPR009010. Asp_de-COase-like_fold. IPR001041. Ferredoxin. IPR006478. Formate_DH_asu. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF00111. Fer2. 1 hit. PF00037. Fer4. 2 hits. PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. PF10588. NADH-G_4Fe-4S_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01591. Fdh-alpha. 1 hit.
PROSITE	PS51085. 2FE2S_FER_2. 1 hit. PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 2 hits. PS00551. MOLYBDOPTERIN_PROK_1. False negative. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDHL_BACSU

Accession

Primary (citable) accession number: Q795Y4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)