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Q795Y4 (FDHL_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative formate dehydrogenase YrhE

EC=1.2.1.2
Gene names
Name:yrhE
Ordered Locus Names:BSU27220
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length980 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Formate + NAD+ = CO2 + NADH.

Cofactor

Binds 1 2Fe-2S cluster By similarity.

Binds 4 4Fe-4S clusters By similarity.

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sequence similarities

In the C-terminal section; belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Contains 1 2Fe-2S ferredoxin-type domain.

Contains 2 4Fe-4S ferredoxin-type domains.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 980980Putative formate dehydrogenase YrhE
PRO_0000360689

Regions

Domain5 – 81772Fe-2S ferredoxin-type
Domain144 – 171284Fe-4S ferredoxin-type 1
Domain187 – 216304Fe-4S ferredoxin-type 2
Domain263 – 319574Fe-4S Mo/W bis-MGD-type
Region258 – 980723Formate dehydrogenase By similarity

Sites

Metal binding391Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding501Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding531Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding651Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding971Iron-sulfur 2 (4Fe-4S); via tele nitrogen By similarity
Metal binding1011Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1041Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1111Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1531Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1561Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1591Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1631Iron-sulfur 4 (4Fe-4S) By similarity
Metal binding1961Iron-sulfur 4 (4Fe-4S) By similarity
Metal binding1991Iron-sulfur 4 (4Fe-4S) By similarity
Metal binding2021Iron-sulfur 4 (4Fe-4S) By similarity
Metal binding2061Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding2701Iron-sulfur 5 (4Fe-4S) By similarity
Metal binding2731Iron-sulfur 5 (4Fe-4S) By similarity
Metal binding2771Iron-sulfur 5 (4Fe-4S) By similarity
Metal binding3051Iron-sulfur 5 (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q795Y4 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 0DA2B17A506806ED

FASTA980108,775
        10         20         30         40         50         60 
MMDVKSISVR VDGTEIQARA GATILDILNE NGIEYPQICH VPEVDPIQTC DTCIVEANGK 

        70         80         90        100        110        120 
LVRSCATVAE NGMSIDLSGN RVKEAQTEAM DRLLENHLLY CTVCDNNNGN CTLHNTAEMM 

       130        140        150        160        170        180 
GIEHQKYPYT PKEDPSCAVD MSHPFYRYDP NQCIACGQCV EVCQNLQVNE TLSIDWERER 

       190        200        210        220        230        240 
PRVIWDEGVP INESSCVSCG QCVTVCPCNA LMEKSMLGQA GFMTGIKEDV MEPMIDLVKN 

       250        260        270        280        290        300 
VEPGYGSIFA ISEVEAAMRE TRTKKTKTVC TFCGVGCSFE VWTKGRDILK IQPVSDAPVN 

       310        320        330        340        350        360 
AISTCVKGKF GWDFVNSKER ITKPLIRKNG AFVESSWEEA LDLVASRLGS IKEQYGKGSV 

       370        380        390        400        410        420 
GFISSSKITN EENYVIQKLA RQVFETNNVD NCSRYCQSPA TDGLFRTVGM GGDAGTIKDI 

       430        440        450        460        470        480 
AKAGLVIIVG ANPAEGHPVL ATRVKRAHKL HGQKLIVADL RKNEMAERSD LFISPKQGTD 

       490        500        510        520        530        540 
QVWLMAVTKY MIDQGWHDQA FIDENVNYFE DYKETLKTYT LDYAERITGL SKENIIQIAE 

       550        560        570        580        590        600 
MIRDADGTCV LWGMGVTQNT GGSDTSAAIS NLLLATGNYR RPGAGAYPLR GHNNVQGACD 

       610        620        630        640        650        660 
MGTLPGWLPG YQHITDDKAR AKFEEAYGVE IDGKPGLDNI QMLHAIEEGK MKAMYLVGED 

       670        680        690        700        710        720 
MALVDSNANH VHDILSSLDF FVVQDIFLSR TAQYADVVLP ATPSLEKDGT FTNTERRVQR 

       730        740        750        760        770        780 
LYQALPTLGD AKPDWWIIQE VANRLGANWN YSHPSDIFSE MASLSPLFAK ASYEVLEGWN 

       790        800        810        820        830        840 
SFLWGSFTGE STPLLYEDGF NFPDKKARFA LSDWTEPAAF PEEYDLHINN GRMLEHFHEG 

       850        860        870        880        890        900 
NMTNKSKGIQ AKVPNVFVEI SPELAQERGV CDGSLVRLVS PFGAVKLNAL ITDRVRKNEL 

       910        920        930        940        950        960 
YLPMNSTDKE SAINFLTGPA ADKRTNTPAY KQTKVRMEVL GGCETAPLPK TNPRNKKRHP 

       970        980 
QNGVEAERKW NRPGYVHLTD 

« Hide

References

[1]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL009126 Genomic DNA. Translation: CAB14664.1.
RefSeqNP_390600.1. NC_000964.3.

3D structure databases

ProteinModelPortalQ795Y4.
SMRQ795Y4. Positions 5-218, 262-939.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU27220.

Proteomic databases

PaxDbQ795Y4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB14664; CAB14664; BSU27220.
GeneID937569.
KEGGbsu:BSU27220.
PATRIC18977300. VBIBacSub10457_2839.

Organism-specific databases

GenoListBSU27220.

Phylogenomic databases

eggNOGCOG3383.
HOGENOMHOG000031440.
KOK00123.
OMALDNRSTW.
OrthoDBEOG6CVV7G.
PhylomeDBQ795Y4.

Enzyme and pathway databases

BioCycBSUB:BSU27220-MONOMER.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
InterProIPR001041. 2Fe-2S_ferredoxin-type.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR009010. Asp_de-COase-like_dom.
IPR012675. Beta-grasp_dom.
IPR006478. Formate_DH_asu.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
[Graphical view]
PfamPF13237. Fer4_10. 1 hit.
PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
PIRSFPIRSF036643. FDH_alpha. 1 hit.
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
SM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsTIGR01591. Fdh-alpha. 1 hit.
PROSITEPS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFDHL_BACSU
AccessionPrimary (citable) accession number: Q795Y4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList