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Protein

4F2 cell-surface antigen heavy chain

Gene

Slc3a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the function of light chain amino-acid transporters. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier.3 Publications

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • leucine import Source: UniProtKB
  • neutral amino acid transport Source: RGD
  • tryptophan transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

SABIO-RKQ794F9.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
4F2 cell-surface antigen heavy chain
Short name:
4F2hc
Alternative name(s):
Solute carrier family 3 member 2
CD_antigen: CD98
Gene namesi
Name:Slc3a2Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3073. Slc3a2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7575CytoplasmicSequence analysisAdd
BLAST
Transmembranei76 – 9823Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini99 – 527429ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • melanosome Source: UniProtKB-SubCell
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5275274F2 cell-surface antigen heavy chainPRO_0000252236Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21PhosphoserineCombined sources
Modified residuei5 – 51PhosphothreonineCombined sources
Cross-linki42 – 42Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei58 – 581PhosphoserineBy similarity
Cross-linki59 – 59Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Disulfide bondi103 – 103Interchain (with light chain)By similarity
Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence analysis
Glycosylationi249 – 2491N-linked (GlcNAc...)Sequence analysis
Glycosylationi259 – 2591N-linked (GlcNAc...)Sequence analysis
Glycosylationi263 – 2631N-linked (GlcNAc...)Sequence analysis
Glycosylationi318 – 3181N-linked (GlcNAc...)Sequence analysis
Glycosylationi386 – 3861N-linked (GlcNAc...)Sequence analysis
Glycosylationi400 – 4001N-linked (GlcNAc...)Sequence analysis
Glycosylationi510 – 5101N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Phosphorylation on Ser-300 and on Ser-421 by ecto-protein kinases favors heterotypic cell-cell interactions.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ794F9.
PRIDEiQ794F9.

PTM databases

iPTMnetiQ794F9.
PhosphoSiteiQ794F9.
SwissPalmiQ794F9.
UniCarbKBiQ794F9.

Expressioni

Tissue specificityi

In brain expressed on capillary endothelia in cerebral cortex. Highest expression in kidney, jejunum, ileum, colon, placenta, testis and spleen. Lower levels found in liver, lung and brain with weakest expression in heart. Expressed in retina, inner blood-retinal barrier of retina, retinal vascular endothelial cells. Also expressed in C6 glioma cells and in the retinal capillary endothelial cell line TR-iBRB2.3 Publications

Inductioni

Expression induced in normal hepatic cells in the presence of actinomycin-D.1 Publication

Gene expression databases

BgeeiENSRNOG00000018487.
GenevisibleiQ794F9. RN.

Interactioni

Subunit structurei

Disulfide-linked heterodimer of a glycosylated heavy chain and a non-glycosylated light chain (SLC7A5, SLC7A6, SLCA7A7, SLC7A8, SLC7A10 or SLCA7A11). Colocalizes with cadherins (By similarity). Interacts with FAM57A/CT120 and ICAM1 (By similarity). Constitutively and specifically associates with beta-1 integrins (alpha-2/beta-1, alpha-3/beta-1, alpha-5/beta-1 and alpha-6/beta-1), but minimally with alpha-4/beta-1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi248392. 1 interaction.
IntActiQ794F9. 1 interaction.
STRINGi10116.ENSRNOP00000025196.

Structurei

3D structure databases

ProteinModelPortaliQ794F9.
SMRiQ794F9. Positions 105-527.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SLC3A transporter family.Sequence analysis

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0471. Eukaryota.
COG0366. LUCA.
HOGENOMiHOG000233529.
HOVERGENiHBG000023.
InParanoidiQ794F9.
KOiK06519.
OrthoDBiEOG091G07U6.
PhylomeDBiQ794F9.
TreeFamiTF314498.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR031984. SLC3A2_N.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF16028. SLC3A2_N. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Q794F9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQDTEVDMK DVELNELEPE KQPMNAADGA AAGEKNGLVK IKVAEDEAEA
60 70 80 90 100
GVKFTGLSKE ELLKVAGSPG WVRTRWALLL LFWLGWLGML AGAVVIIVRA
110 120 130 140 150
PRCRELPVQR WWHKGALYRI GDLQAFVGPE ARGIAGLKNH LEYLSTLKVK
160 170 180 190 200
GLVLGPIHKN QKDEVNETDL KQIDPDLGSQ EDFKDLLQSA KKKSIHIILD
210 220 230 240 250
LTPNYKGQNA WFLPPQADIV ATKMKEALSS WLQDGVDGFQ VRDVGKLANA
260 270 280 290 300
SLYLAEWQNI TKNFSEDRLL IAGTASSDLQ QIVNILESTS DLLLTSSYLS
310 320 330 340 350
QPVFTGEHAE LLVIKYLNAT GSRWCSWSVS QAGLLTSFIP AQFLRLYQLL
360 370 380 390 400
LFTLPGTPVF SYGDELGLQA VALPGQPMEA PFMLWNESSN SQTSSPVSLN
410 420 430 440 450
MTVKGQNEDP GSLLTQFRRL SDLRGKERSL LHGDFDALSS SSGLFSYVRH
460 470 480 490 500
WDQNERYLVV LNFQDVGLSA RVGASNLPAG ISLPASANLL LSTDSTRLSR
510 520
EEGTSLSLEN LSLNPYEGLL LQFPFVA
Length:527
Mass (Da):58,072
Last modified:July 5, 2004 - v1
Checksum:iCBA92D830893672B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59324 mRNA. Translation: AAC53560.1.
AB015433 mRNA. Translation: BAA33036.1.
BC061989 mRNA. Translation: AAH61989.1.
RefSeqiNP_001258018.1. NM_001271089.2.
UniGeneiRn.5801.

Genome annotation databases

GeneIDi50567.
KEGGirno:50567.
UCSCiRGD:3073. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59324 mRNA. Translation: AAC53560.1.
AB015433 mRNA. Translation: BAA33036.1.
BC061989 mRNA. Translation: AAH61989.1.
RefSeqiNP_001258018.1. NM_001271089.2.
UniGeneiRn.5801.

3D structure databases

ProteinModelPortaliQ794F9.
SMRiQ794F9. Positions 105-527.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248392. 1 interaction.
IntActiQ794F9. 1 interaction.
STRINGi10116.ENSRNOP00000025196.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

PTM databases

iPTMnetiQ794F9.
PhosphoSiteiQ794F9.
SwissPalmiQ794F9.
UniCarbKBiQ794F9.

Proteomic databases

PaxDbiQ794F9.
PRIDEiQ794F9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi50567.
KEGGirno:50567.
UCSCiRGD:3073. rat.

Organism-specific databases

CTDi6520.
RGDi3073. Slc3a2.

Phylogenomic databases

eggNOGiKOG0471. Eukaryota.
COG0366. LUCA.
HOGENOMiHOG000233529.
HOVERGENiHBG000023.
InParanoidiQ794F9.
KOiK06519.
OrthoDBiEOG091G07U6.
PhylomeDBiQ794F9.
TreeFamiTF314498.

Enzyme and pathway databases

SABIO-RKQ794F9.

Miscellaneous databases

PROiQ794F9.

Gene expression databases

BgeeiENSRNOG00000018487.
GenevisibleiQ794F9. RN.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR031984. SLC3A2_N.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF16028. SLC3A2_N. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry namei4F2_RAT
AccessioniPrimary (citable) accession number: Q794F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.