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Protein

Heterogeneous nuclear ribonucleoprotein F

Gene

Hnrnpf

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state (By similarity).By similarity

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: Ensembl
  • single-stranded RNA binding Source: UniProtKB
  • TBP-class protein binding Source: RGD
  • transcription factor binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-6803529. FGFR2 alternative splicing.
R-RNO-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoprotein F
Short name:
hnRNP F
Cleaved into the following chain:
Gene namesi
Name:Hnrnpf
Synonyms:Hnrpf
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi620366. Hnrnpf.

Subcellular locationi

  • Nucleusnucleoplasm 1 Publication

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: Ensembl
  • cytoplasm Source: Ensembl
  • membrane Source: Ensembl
  • nucleoplasm Source: UniProtKB-SubCell
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003671171 – 415Heterogeneous nuclear ribonucleoprotein FAdd BLAST415
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00002530542 – 415Heterogeneous nuclear ribonucleoprotein F, N-terminally processedAdd BLAST414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylmethionine; in Heterogeneous nuclear ribonucleoprotein F, N-terminally processedBy similarity1
Cross-linki72Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei104PhosphoserineCombined sources1
Modified residuei107PhosphoserineBy similarity1
Modified residuei161PhosphoserineBy similarity1
Modified residuei187PhosphoserineBy similarity1
Modified residuei193PhosphoserineBy similarity1
Modified residuei195PhosphoserineCombined sources1
Modified residuei200N6-acetyllysine; alternateBy similarity1
Cross-linki200Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei215PhosphothreonineBy similarity1
Modified residuei224N6-acetyllysineBy similarity1
Modified residuei265PhosphoserineBy similarity1

Post-translational modificationi

Sumoylated.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ794E4.
PRIDEiQ794E4.

PTM databases

iPTMnetiQ794E4.
PhosphoSitePlusiQ794E4.

Expressioni

Tissue specificityi

Detected in liver, thymus, spleen and testis.1 Publication

Gene expression databases

BgeeiENSRNOG00000014562.
GenevisibleiQ794E4. RN.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Interacts with AGO1, AGO2, TBP and TXNL4/DIM1 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei16Interaction with RNABy similarity1
Sitei20Interaction with RNABy similarity1
Sitei52Interaction with RNABy similarity1
Sitei75Interaction with RNABy similarity1
Sitei116Interaction with RNABy similarity1
Sitei120Interaction with RNABy similarity1
Sitei150Interaction with RNABy similarity1
Sitei173Interaction with RNABy similarity1
Sitei294Interaction with RNABy similarity1
Sitei298Interaction with RNABy similarity1
Sitei326Interaction with RNABy similarity1
Sitei349Interaction with RNABy similarity1

GO - Molecular functioni

  • TBP-class protein binding Source: RGD
  • transcription factor binding Source: RGD

Protein-protein interaction databases

BioGridi249006. 1 interactor.
IntActiQ794E4. 1 interactor.
STRINGi10116.ENSRNOP00000019529.

Structurei

3D structure databases

ProteinModelPortaliQ794E4.
SMRiQ794E4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 90RRM 1PROSITE-ProRule annotationAdd BLAST80
Domaini111 – 188RRM 2PROSITE-ProRule annotationAdd BLAST78
Domaini289 – 366RRM 3PROSITE-ProRule annotationAdd BLAST78

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni81 – 86Interaction with RNABy similarity6
Regioni179 – 184Interaction with RNABy similarity6
Regioni355 – 360Interaction with RNABy similarity6

Domaini

The N-terminal RRM domains are responsible for recognizing the G-tract of BCL-X RNA.By similarity

Sequence similaritiesi

Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4211. Eukaryota.
ENOG410Z6M0. LUCA.
GeneTreeiENSGT00760000119102.
HOGENOMiHOG000220896.
HOVERGENiHBG055557.
InParanoidiQ794E4.
KOiK12898.
OMAiFLSECKI.
PhylomeDBiQ794E4.
TreeFamiTF316157.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR012996. Znf_CHHC.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
PF08080. zf-RNPHF. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q794E4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLGPEGGEG YVVKLRGLPW SCSIEDVQNF LSDCTIHDGV AGVHFIYTRE
60 70 80 90 100
GRQSGEAFVE LESEDDVKLA LKKDRESMGH RYIEVFKSHR TEMDWVLKHS
110 120 130 140 150
GPNSADSAND GFVRLRGLPF GCTKEEIVQF FSGLEIVPNG ITLPVDPEGK
160 170 180 190 200
ITGEAFVQFA SQELAEKALG KHKERIGHRY IEVFKSSQEE VRSYSDPPLK
210 220 230 240 250
FMSVQRPGPY DRPGTARRYI GIVKQAGLDR MRSGAYSAGY GGYEEYSGLS
260 270 280 290 300
DGYGFTTDLF GRDLSYCLSG MYDHRYGDSE FTVQSTTGHC VHMRGLPYKA
310 320 330 340 350
TENDIYNFFS PLNPVRVHIE IGPDGRVTGE ADVEFATHEE AVAAMSKDRA
360 370 380 390 400
NMQHRYIELF LNSTTGASNG AYSSQVMQGM GVSAAQATYS GLESQSVSGC
410
YGAGYSGQNS MGGYD
Length:415
Mass (Da):45,730
Last modified:January 23, 2007 - v3
Checksum:i31E1C9C90A8E051F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022209 mRNA. Translation: BAA37095.1.
BC097275 mRNA. Translation: AAH97275.1.
BC103634 mRNA. Translation: AAI03635.1.
RefSeqiNP_001032362.1. NM_001037285.1.
NP_001032363.1. NM_001037286.1.
NP_001032364.1. NM_001037287.1.
NP_071792.1. NM_022397.3.
XP_008761484.1. XM_008763262.2.
XP_017448358.1. XM_017592869.1.
XP_017448359.1. XM_017592870.1.
XP_017448360.1. XM_017592871.1.
XP_017448361.1. XM_017592872.1.
XP_017448362.1. XM_017592873.1.
XP_017448363.1. XM_017592874.1.
XP_017448364.1. XM_017592875.1.
UniGeneiRn.144596.

Genome annotation databases

EnsembliENSRNOT00000019529; ENSRNOP00000019529; ENSRNOG00000014562.
ENSRNOT00000083843; ENSRNOP00000068652; ENSRNOG00000014562.
ENSRNOT00000091765; ENSRNOP00000069655; ENSRNOG00000014562.
GeneIDi64200.
KEGGirno:64200.
UCSCiRGD:620366. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022209 mRNA. Translation: BAA37095.1.
BC097275 mRNA. Translation: AAH97275.1.
BC103634 mRNA. Translation: AAI03635.1.
RefSeqiNP_001032362.1. NM_001037285.1.
NP_001032363.1. NM_001037286.1.
NP_001032364.1. NM_001037287.1.
NP_071792.1. NM_022397.3.
XP_008761484.1. XM_008763262.2.
XP_017448358.1. XM_017592869.1.
XP_017448359.1. XM_017592870.1.
XP_017448360.1. XM_017592871.1.
XP_017448361.1. XM_017592872.1.
XP_017448362.1. XM_017592873.1.
XP_017448363.1. XM_017592874.1.
XP_017448364.1. XM_017592875.1.
UniGeneiRn.144596.

3D structure databases

ProteinModelPortaliQ794E4.
SMRiQ794E4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249006. 1 interactor.
IntActiQ794E4. 1 interactor.
STRINGi10116.ENSRNOP00000019529.

PTM databases

iPTMnetiQ794E4.
PhosphoSitePlusiQ794E4.

Proteomic databases

PaxDbiQ794E4.
PRIDEiQ794E4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019529; ENSRNOP00000019529; ENSRNOG00000014562.
ENSRNOT00000083843; ENSRNOP00000068652; ENSRNOG00000014562.
ENSRNOT00000091765; ENSRNOP00000069655; ENSRNOG00000014562.
GeneIDi64200.
KEGGirno:64200.
UCSCiRGD:620366. rat.

Organism-specific databases

CTDi3185.
RGDi620366. Hnrnpf.

Phylogenomic databases

eggNOGiKOG4211. Eukaryota.
ENOG410Z6M0. LUCA.
GeneTreeiENSGT00760000119102.
HOGENOMiHOG000220896.
HOVERGENiHBG055557.
InParanoidiQ794E4.
KOiK12898.
OMAiFLSECKI.
PhylomeDBiQ794E4.
TreeFamiTF316157.

Enzyme and pathway databases

ReactomeiR-RNO-6803529. FGFR2 alternative splicing.
R-RNO-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

PROiQ794E4.

Gene expression databases

BgeeiENSRNOG00000014562.
GenevisibleiQ794E4. RN.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR012996. Znf_CHHC.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
PF08080. zf-RNPHF. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHNRPF_RAT
AccessioniPrimary (citable) accession number: Q794E4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.