Q793F9 (VPS4A_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified July 9, 2014. Version 80. History...
Names and origin
|Protein names||Recommended name:|
Vacuolar protein sorting-associated protein 4A
|Organism||Rattus norvegicus (Rat) [Reference proteome]|
|Taxonomic identifier||10116 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus|
|Sequence length||437 AA.|
|Sequence processing||The displayed sequence is further processed into a mature form.|
|Protein existence||Evidence at transcript level|
General annotation (Comments)
Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission By similarity.
ATP + H2O = ADP + phosphate.
Proposed to be monomeric or homodimeric in nucleotide-free form and to oligomerize upon binding to ATP to form two stacked hexameric or heptameric rings with a central pore through which ESCRT-III substrates are translocated in an ATP-dependent manner. Interacts with CHMP1A, CHMP1B, CHMP2A, CHMP2B, CHMP3, CHMP4A, CHMP4B, CHMP4C and CHMP6. Interacts with VPS4B; the interaction suggests a heteromeric assembly with VPS4B. Interacts with SPAST. Interacts with IST1. Interacts with ZFYVE19/ANCHR; leading to retain it at midbody By similarity.
Prevacuolar compartment membrane; Peripheral membrane protein. Late endosome membrane; Peripheral membrane protein By similarity. Midbody By similarity. Note: Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells. Localizes to the midbody of dividing cells, interaction with ZFYVE19/ANCHR mediates retention at midbody. Localized in two distinct rings on either side of the Fleming body By similarity.
The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2) By similarity.
Belongs to the AAA ATPase family.
Contains 1 MIT domain.
|Biological process||Cell cycle|
|Technical term||Complete proteome|
|Gene Ontology (GO)|
Inferred from electronic annotation. Source: UniProtKB-KWcell division
Inferred from electronic annotation. Source: UniProtKB-KWendosomal transportprotein transport
Inferred from electronic annotation. Source: UniProtKB-KW
|Cellular_component||early endosomelate endosome membrane|
Inferred from electronic annotation. Source: UniProtKB-SubCell
Inferred from electronic annotation. Source: UniProtKB-KWRho GTPase bindingnucleoside-triphosphatase activity
Inferred from electronic annotation. Source: InterPro
|Complete GO annotation...|
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Initiator methionine||1||1||Removed By similarity|
|Chain||2 – 437||436||Vacuolar protein sorting-associated protein 4A||PRO_0000331377|
|Domain||2 – 80||79||MIT|
|Nucleotide binding||167 – 174||8||ATP Potential|
|Coiled coil||15 – 37||23||Potential|
Amino acid modifications
|Modified residue||2||1||N-acetylthreonine By similarity|
|Modified residue||8||1||N6-acetyllysine By similarity|
|AB076398 mRNA. Translation: BAC00961.1.|
|RefSeq||NP_663711.1. NM_145678.1. |
3D structure databases
|SMR||Q793F9. Positions 3-101, 116-436. |
Protocols and materials databases
Genome annotation databases
|Ensembl||ENSRNOT00000027607; ENSRNOP00000027607; ENSRNOG00000020351. |
|UCSC||RGD:628810. rat. |
|RGD||628810. Vps4a. |
Gene expression databases
Family and domain databases
|Gene3D||126.96.36.1990. 1 hit. |
188.8.131.520. 1 hit.
|InterPro||IPR003593. AAA+_ATPase. |
|Pfam||PF00004. AAA. 1 hit. |
PF04212. MIT. 1 hit.
PF09336. Vps4_C. 1 hit.
|SMART||SM00382. AAA. 1 hit. |
SM00745. MIT. 1 hit.
|SUPFAM||SSF116846. SSF116846. 1 hit. |
SSF52540. SSF52540. 1 hit.
|PROSITE||PS00674. AAA. 1 hit. |
|Accession||Primary (citable) accession number: Q793F9|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|
Index of protein domains and families