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Q793F9

- VPS4A_RAT

UniProt

Q793F9 - VPS4A_RAT

Protein

Vacuolar protein sorting-associated protein 4A

Gene

Vps4a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission By similarity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi167 – 1748ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. nucleoside-triphosphatase activity Source: InterPro
    3. Rho GTPase binding Source: RGD

    GO - Biological processi

    1. abscission Source: UniProtKB
    2. cell division Source: UniProtKB-KW
    3. cytokinesis checkpoint Source: UniProtKB
    4. endosomal transport Source: RGD
    5. negative regulation of cytokinesis Source: UniProtKB
    6. protein transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Cell cycle, Cell division, Protein transport, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_196252. Endosomal Sorting Complex Required For Transport (ESCRT).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar protein sorting-associated protein 4A (EC:3.6.4.6)
    Gene namesi
    Name:Vps4a
    Synonyms:vps4-A
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 19

    Organism-specific databases

    RGDi628810. Vps4a.

    Subcellular locationi

    Prevacuolar compartment membrane; Peripheral membrane protein. Late endosome membrane By similarity; Peripheral membrane protein By similarity. Midbody By similarity
    Note: Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells. Localizes to the midbody of dividing cells, interaction with ZFYVE19/ANCHR mediates retention at midbody. Localized in two distinct rings on either side of the Fleming body By similarity.By similarity

    GO - Cellular componenti

    1. early endosome Source: RGD
    2. late endosome membrane Source: UniProtKB-SubCell
    3. midbody Source: UniProtKB

    Keywords - Cellular componenti

    Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 437436Vacuolar protein sorting-associated protein 4APRO_0000331377Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylthreonineBy similarity
    Modified residuei8 – 81N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ793F9.
    PRIDEiQ793F9.

    PTM databases

    PhosphoSiteiQ793F9.

    Expressioni

    Gene expression databases

    GenevestigatoriQ793F9.

    Interactioni

    Subunit structurei

    Proposed to be monomeric or homodimeric in nucleotide-free form and to oligomerize upon binding to ATP to form two stacked hexameric or heptameric rings with a central pore through which ESCRT-III substrates are translocated in an ATP-dependent manner. Interacts with CHMP1A, CHMP1B, CHMP2A, CHMP2B, CHMP3, CHMP4A, CHMP4B, CHMP4C and CHMP6. Interacts with VPS4B; the interaction suggests a heteromeric assembly with VPS4B. Interacts with SPAST. Interacts with IST1. Interacts with ZFYVE19/ANCHR; leading to retain it at midbody By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ793F9.
    SMRiQ793F9. Positions 3-101, 116-436.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 8079MITAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili15 – 3723Sequence AnalysisAdd
    BLAST

    Domaini

    The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2) By similarity.By similarity

    Sequence similaritiesi

    Belongs to the AAA ATPase family.Curated
    Contains 1 MIT domain.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG0464.
    GeneTreeiENSGT00550000074466.
    HOGENOMiHOG000225146.
    HOVERGENiHBG057074.
    KOiK12196.

    Family and domain databases

    Gene3Di1.20.58.280. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR007330. MIT.
    IPR027417. P-loop_NTPase.
    IPR015415. Vps4_C.
    [Graphical view]
    PfamiPF00004. AAA. 1 hit.
    PF04212. MIT. 1 hit.
    PF09336. Vps4_C. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    SM00745. MIT. 1 hit.
    [Graphical view]
    SUPFAMiSSF116846. SSF116846. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS00674. AAA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q793F9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTSTLQKAI DLVTKATEED KAKNYEEALR LYQHAVEYFL HAIKYEAHSD    50
    KAKESIRAKC MQYLDRAEKL KDYLRNKEKH GKKPVKENQS EGKGSDSDSE 100
    GDNPEKKKLQ EQLMGAVVME KPNIRWNDVA GLEGAKEALK EAVILPIKFP 150
    HLFTGKRTPW RGILLFGPPG TGKSYLAKAV ATEANNSTFF SVSSSDLMSK 200
    WLGESEKLVK NLFELARQHK PSIIFIDEVD SLCGSRNENE SEAARRIKTE 250
    FLVQMQGVGN NNDGTLVLGA TNIPWVLDSA IRRRFEKRIY IPLPEEAARA 300
    QMFRLHLGST PHNLTDANIH ELARKTEGYS GADISIIVRD SLMQPVRKVQ 350
    SATHFKKVCG PSRTNPSVMI DDLLTPCSPG DPGAIEMTWM DVPGDKLLEP 400
    VVCMSDMLRS LATTRPTVNA DDLLKVKKFS EDFGQES 437
    Length:437
    Mass (Da):48,907
    Last modified:July 5, 2004 - v1
    Checksum:iB67CF13F7AFD9129
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB076398 mRNA. Translation: BAC00961.1.
    RefSeqiNP_663711.1. NM_145678.1.
    UniGeneiRn.12477.

    Genome annotation databases

    EnsembliENSRNOT00000027607; ENSRNOP00000027607; ENSRNOG00000020351.
    GeneIDi246772.
    KEGGirno:246772.
    UCSCiRGD:628810. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB076398 mRNA. Translation: BAC00961.1 .
    RefSeqi NP_663711.1. NM_145678.1.
    UniGenei Rn.12477.

    3D structure databases

    ProteinModelPortali Q793F9.
    SMRi Q793F9. Positions 3-101, 116-436.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q793F9.

    Proteomic databases

    PaxDbi Q793F9.
    PRIDEi Q793F9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000027607 ; ENSRNOP00000027607 ; ENSRNOG00000020351 .
    GeneIDi 246772.
    KEGGi rno:246772.
    UCSCi RGD:628810. rat.

    Organism-specific databases

    CTDi 27183.
    RGDi 628810. Vps4a.

    Phylogenomic databases

    eggNOGi COG0464.
    GeneTreei ENSGT00550000074466.
    HOGENOMi HOG000225146.
    HOVERGENi HBG057074.
    KOi K12196.

    Enzyme and pathway databases

    Reactomei REACT_196252. Endosomal Sorting Complex Required For Transport (ESCRT).

    Miscellaneous databases

    NextBioi 623855.
    PROi Q793F9.

    Gene expression databases

    Genevestigatori Q793F9.

    Family and domain databases

    Gene3Di 1.20.58.280. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR007330. MIT.
    IPR027417. P-loop_NTPase.
    IPR015415. Vps4_C.
    [Graphical view ]
    Pfami PF00004. AAA. 1 hit.
    PF04212. MIT. 1 hit.
    PF09336. Vps4_C. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    SM00745. MIT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF116846. SSF116846. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS00674. AAA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel Rho family GTPase, Rnd2, is linked to endosomal trafficking via direct binding to Vps4-A."
      Tanaka H., Fujita H., Katoh H., Mori K., Negishi M.
      Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiVPS4A_RAT
    AccessioniPrimary (citable) accession number: Q793F9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3