Reviewed, UniProtKB/Swiss-Prot Q792Q4 (CRIPT_RAT)

Names and origin

Protein names

Recommended name:
    Cysteine-rich PDZ-binding protein
Alternative name(s):
    Cysteine-rich interactor of PDZ three
    Cysteine-rich interactor of PDZ3

Gene names

Name:

Cript

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	101 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the cytoskeletal anchoring of DLG4 in excitatory synapses.
Subunit structure	Interacts with DLG4 and TUBB1. Interacts strongly with the PDZ3 domain of members of the DLG4 family. Associates with microtubules.
Subcellular location	Cytoplasm. Cell junction › synapse. Cell projection › dendritic spine. Note= Colocalizes with DLG4 in asymmetric synapses.
Tissue specificity	Expressed in striatum, cortex, midbrain, Purkinje cells of the cerebellum, pyramidal neurons of the hippocampus and neuropil. Expressed in heart, brain, lung, liver, kidney and testis.
Sequence similarities	Belongs to the CRIPT family.

Ontologies

Keywords
Cellular component	Cell junction Cell projection Cytoplasm Synapse
Gene Ontology (GO)
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW cell projection Inferred from electronic annotation. Source: UniProtKB-KW cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW synapse Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Dlg4	P31016	1	EBI-632062,EBI-375655

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 101	101	Cysteine-rich PDZ-binding protein		PRO_0000314565
Regions
	Region	95 – 101	7	Sufficient for interaction with DLG4
	Region	98 – 101	4	PDZ3-binding
Experimental info
	Mutagenesis	98	1	Q → E: Abolishes interaction with DLG4. Strongly abolishes interaction with DLG4; when associated with D-100
	Mutagenesis	100	1	S → D: Abolishes interaction with DLG4. Strongly abolishes interaction with DLG4; when associated with E-98
	Mutagenesis	101	1	V → A: Abolishes interaction with DLG4. Does not redistribute DLG4 to microtubules. Associates with microtubules

Sequences

	Sequence		Length	Mass (Da)
	Q792Q4-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 161D694AC81BFDCB	FASTA	101	11,271
	10 20 30 40 50 60 MVCEKCEKKL GRVITPDTWK DGARNTTESG GRKLNENKAL TSKKARFDPY GKNKFSTCRI 70 80 90 100 CKSSVHQPGS HYCQGCAYKK GICAMCGKKV LDTKNYKQTS V

References

[1]	"CRIPT, a novel postsynaptic protein that binds to the third PDZ domain of PSD-95/SAP90." Niethammer M., Valtschanoff J.G., Kapoor T.M., Allison D.W., Weinberg R.J., Craig A.M., Sheng M. Neuron 20:693-707(1998) [PubMed: 9581762] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH DLG4 AND TUBB1, MUTAGENESIS OF GLN-98; SER-100 AND VAL-101, TISSUE SPECIFICITY.
[2]	"Microtubule binding by CRIPT and its potential role in the synaptic clustering of PSD-95." Passafaro M., Sala C., Niethammer M., Sheng M. Nat. Neurosci. 2:1063-1069(1999) [PubMed: 10570482] [Abstract] Cited for: FUNCTION, ASSOCIATION WITH MICROTUBULE, SUBCELLULAR LOCATION, MUTAGENESIS OF VAL-101.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF047384 mRNA. Translation: AAC40102.1.
RefSeq	NP_063972.1.
UniGene	Rn.30508
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q792Q4.
Genome annotation databases
GeneID	56725.
KEGG	rno:56725.
Organism-specific databases
RGD	621545. Cript.
Phylogenomic databases
HOVERGEN	Q792Q4.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	611134.

Entry information

Entry name

CRIPT_RAT

Accession

Primary (citable) accession number: Q792Q4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)