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Protein

CUGBP Elav-like family member 2

Gene

Celf2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre-mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury. Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression. Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU-rich sequences located immediatly upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA. Binds to an intronic RNA element responsible for the silencing of exon 21 splicing. Binds to (CUG)n repeats (By similarity).By similarity

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • RNA binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CUGBP Elav-like family member 2
Short name:
CELF-2
Alternative name(s):
Bruno-like protein 3
CUG triplet repeat RNA-binding protein 2
Short name:
CUG-BP2
CUG-BP- and ETR-3-like factor 2
ELAV-type RNA-binding protein 3
Short name:
ETR-3
Short name:
Protein ETR-R3
Neuroblastoma apoptosis-related RNA-binding protein
Short name:
rNapor
RNA-binding protein BRUNOL-3
Gene namesi
Name:Celf2
Synonyms:Cugbp2, Etr3, Napor
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68347. Celf2.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Accumulates in the cytoplasm after ionizing radiation. Colocalizes with APOBEC1 and A1CF. RNA-binding activity is detected in both nuclear and cytoplasmic compartments (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 508508CUGBP Elav-like family member 2PRO_0000295192Add
BLAST

Proteomic databases

PaxDbiQ792H5.
PRIDEiQ792H5.

PTM databases

iPTMnetiQ792H5.
PhosphoSiteiQ792H5.

Expressioni

Tissue specificityi

Strongly expressed in forebrain regions, including the cerebral cortex and hippocampus. Moderately expressed in hindbrain regions, including the cerebellum and spinal cord.1 Publication

Interactioni

Subunit structurei

Interacts with A1CF.By similarity

Protein-protein interaction databases

BioGridi248076. 1 interaction.
STRINGi10116.ENSRNOP00000023436.

Structurei

3D structure databases

ProteinModelPortaliQ792H5.
SMRiQ792H5. Positions 6-211, 388-508.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 12384RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini132 – 21281RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini423 – 50179RRM 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 283283Necessary for RNA-binding, TNNT2 exon 5 and NMDA R1 exon 21 inclusionBy similarityAdd
BLAST
Regioni357 – 508152Necessary for RNA-binding, TNNT2 exon 5 and NMDA R1 exon 21 inclusionBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi284 – 397114Ala-richAdd
BLAST

Sequence similaritiesi

Belongs to the CELF/BRUNOL family.Curated
Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0146. Eukaryota.
ENOG410XNTW. LUCA.
HOGENOMiHOG000004754.
HOVERGENiHBG107646.
InParanoidiQ792H5.
KOiK13207.
PhylomeDBiQ792H5.
TreeFamiTF314924.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR002343. Hud_Sxl_RNA.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 3 hits.
[Graphical view]
PRINTSiPR00961. HUDSXLRNA.
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q792H5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRCPKSAVTM RNEELLLSNG TANKMNGALD HSDQPDPDAI KMFVGQIPRS
60 70 80 90 100
WSEKELKELF EPYGAVYQIN VLRDRSQNPP QSKGCCFVTF YTRKAALEAQ
110 120 130 140 150
NALHNIKTLP GMHHPIQMKP ADSEKSNAVE DRKLFIGMVS KKCNENDIRV
160 170 180 190 200
MFSPFGQIEE CRILRGPDGL SRGCAFVTFS TRAMAQNAIK AMHQSQTMEG
210 220 230 240 250
CSSPIVVKFA DTQKDKEQRR LQQQLAQQMQ QLNTATWGNL TGLGGLTPQY
260 270 280 290 300
LALLQQATSS SNLGAFSGIQ QMAGMNALQL QNLATLAAAA AAAQTSATST
310 320 330 340 350
NANPLSSTSS ALGALTSPVA ASTPNSTAGA AMNSLTSLGT LQGLAGATVG
360 370 380 390 400
LNNINALAGM AALNGGLGAT GLTNGTAGTM DALTQAYSGI QQYAAAALPT
410 420 430 440 450
LYSQSLLQQQ SAAGSQKEGP EGANLFIYHL PQEFGDQDIL QMFMPFGNVI
460 470 480 490 500
SAKVFIDKQT NLSKCFGFVS YDNPVSAQAA IQAMNGFQIG MKRLKVQLKR

SKNDSKPY
Length:508
Mass (Da):54,271
Last modified:July 5, 2004 - v1
Checksum:iC35CBEF598749A79
GO
Isoform 2 (identifier: Q792H5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-24: Missing.

Show »
Length:484
Mass (Da):51,625
Checksum:i5C63F3D4F7203E56
GO
Isoform 3 (identifier: Q792H5-3) [UniParc]FASTAAdd to basket
Also known as: ETR-R3a

The sequence of this isoform differs from the canonical sequence as follows:
     1-24: Missing.
     358-358: A → AVAQMLS

Show »
Length:490
Mass (Da):52,254
Checksum:iE83296A0F13D90BE
GO
Isoform 4 (identifier: Q792H5-4) [UniParc]FASTAAdd to basket
Also known as: ETR-R3b

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MFERTSELAFVETISVESM
     358-358: A → AVAQMLS

Show »
Length:532
Mass (Da):56,958
Checksum:i120FF8EEC82C248F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2424Missing in isoform 2 and isoform 3. 2 PublicationsVSP_026813Add
BLAST
Alternative sequencei1 – 11M → MFERTSELAFVETISVESM in isoform 4. 1 PublicationVSP_026814
Alternative sequencei358 – 3581A → AVAQMLS in isoform 3 and isoform 4. 1 PublicationVSP_026815

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090695 mRNA. Translation: AAD13762.1.
AJ010351 mRNA. Translation: CAA09102.1.
AJ010386 mRNA. Translation: CAA09103.1.
BC129096 mRNA. Translation: AAI29097.1.
RefSeqiNP_058893.2. NM_017197.3. [Q792H5-4]
XP_008770083.1. XM_008771861.1. [Q792H5-4]
XP_008770084.1. XM_008771862.1. [Q792H5-3]
XP_008770086.1. XM_008771864.1. [Q792H5-2]
UniGeneiRn.162247.
Rn.234364.

Genome annotation databases

GeneIDi29428.
KEGGirno:29428.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF090695 mRNA. Translation: AAD13762.1.
AJ010351 mRNA. Translation: CAA09102.1.
AJ010386 mRNA. Translation: CAA09103.1.
BC129096 mRNA. Translation: AAI29097.1.
RefSeqiNP_058893.2. NM_017197.3. [Q792H5-4]
XP_008770083.1. XM_008771861.1. [Q792H5-4]
XP_008770084.1. XM_008771862.1. [Q792H5-3]
XP_008770086.1. XM_008771864.1. [Q792H5-2]
UniGeneiRn.162247.
Rn.234364.

3D structure databases

ProteinModelPortaliQ792H5.
SMRiQ792H5. Positions 6-211, 388-508.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248076. 1 interaction.
STRINGi10116.ENSRNOP00000023436.

PTM databases

iPTMnetiQ792H5.
PhosphoSiteiQ792H5.

Proteomic databases

PaxDbiQ792H5.
PRIDEiQ792H5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29428.
KEGGirno:29428.

Organism-specific databases

CTDi10659.
RGDi68347. Celf2.

Phylogenomic databases

eggNOGiKOG0146. Eukaryota.
ENOG410XNTW. LUCA.
HOGENOMiHOG000004754.
HOVERGENiHBG107646.
InParanoidiQ792H5.
KOiK13207.
PhylomeDBiQ792H5.
TreeFamiTF314924.

Miscellaneous databases

PROiQ792H5.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR002343. Hud_Sxl_RNA.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 3 hits.
[Graphical view]
PRINTSiPR00961. HUDSXLRNA.
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCELF2_RAT
AccessioniPrimary (citable) accession number: Q792H5
Secondary accession number(s): A1L1J5, O88756, Q78ZF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 5, 2004
Last modified: June 8, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.