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Protein

Nuclear distribution protein nudE-like 1

Gene

Ndel1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL (By similarity). Plays a role, together with DISC1, in the regulation of neurite outgrowth.By similarity1 Publication

GO - Molecular functioni

  • microtubule binding Source: GO_Central
  • oligopeptidase activity Source: RGD
  • protein complex binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis, Transport

Enzyme and pathway databases

ReactomeiR-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-5663220. RHO GTPases Activate Formins.
R-RNO-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear distribution protein nudE-like 1
Short name:
Protein Nudel
Gene namesi
Name:Ndel1
Synonyms:Nude2, Nudel
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi621235. Ndel1.

Subcellular locationi

GO - Cellular componenti

  • axon Source: RGD
  • axon cytoplasm Source: GOC
  • axon hillock Source: Ensembl
  • cell body Source: RGD
  • cell leading edge Source: Ensembl
  • centrosome Source: UniProtKB
  • condensed chromosome kinetochore Source: UniProtKB-SubCell
  • cytoplasm Source: RGD
  • kinesin complex Source: RGD
  • kinetochore Source: GO_Central
  • microtubule Source: UniProtKB-KW
  • neurofilament cytoskeleton Source: Ensembl
  • nuclear envelope Source: MGI
  • spindle Source: UniProtKB-SubCell
  • synaptic vesicle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002402151 – 345Nuclear distribution protein nudE-like 1Add BLAST345

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei215PhosphoserineBy similarity1
Modified residuei219Phosphothreonine; by CDK1 and MAPK1By similarity1
Modified residuei231PhosphoserineBy similarity1
Modified residuei242Phosphoserine; by CDK1By similarity1
Modified residuei245Phosphothreonine; by CDK1 and MAPK1By similarity1
Lipidationi273S-palmitoyl cysteine; by ZDHHC2, ZDHHC3 and ZDHHC7By similarity1
Modified residuei344PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE (By similarity).By similarity
Palmitoylation at Cys-273 reduces affinity for dynein.By similarity

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ78PB6.
PRIDEiQ78PB6.

PTM databases

iPTMnetiQ78PB6.
PhosphoSitePlusiQ78PB6.

Expressioni

Inductioni

Up-regulated during neurite outgrowth upon differentiation with NGF.1 Publication

Gene expression databases

BgeeiENSRNOG00000004139.
GenevisibleiQ78PB6. RN.

Interactioni

Subunit structurei

Self-associates. Interacts with dynactin, tubulin gamma, KATNA1, KATNB1, microtubules, PCM1, PCNT, and YWHAE. Interacts directly with NEFL and indirectly with NEFH. Interacts (via C-terminus) with CENPF (By similarity). Interacts with DISC1, dynein and PAFAH1B1. Interacts with ZNF365 (By similarity).By similarity

GO - Molecular functioni

  • microtubule binding Source: GO_Central
  • protein complex binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005574.

Structurei

Secondary structure

1345
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 164Combined sources153

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V71X-ray2.24A/B8-193[»]
ProteinModelPortaliQ78PB6.
SMRiQ78PB6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ78PB6.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni56 – 166Self-associationBy similarityAdd BLAST111
Regioni64 – 189Interaction with KATNB1By similarityAdd BLAST126
Regioni114 – 133Required for interaction with PAFAH1B1By similarityAdd BLAST20
Regioni175 – 345Interaction with CENPFBy similarityAdd BLAST171
Regioni189 – 256Interaction with YWHAEBy similarityAdd BLAST68
Regioni191 – 345Interaction with NEFLBy similarityAdd BLAST155
Regioni195 – 256Interaction with KATNA1By similarityAdd BLAST62
Regioni227 – 278Interaction with DISC1By similarityAdd BLAST52
Regioni256 – 291Required for localization to the centrosome and interaction with dynein, dynactin, tubulin gamma, PCM1 and PCNTBy similarityAdd BLAST36

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili28 – 190Sequence analysisAdd BLAST163

Sequence similaritiesi

Belongs to the nudE family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1853. Eukaryota.
ENOG410XPMP. LUCA.
GeneTreeiENSGT00390000000111.
HOGENOMiHOG000280681.
HOVERGENiHBG082010.
InParanoidiQ78PB6.
KOiK16739.
OMAiQAFLIMD.
OrthoDBiEOG091G0GBV.
PhylomeDBiQ78PB6.
TreeFamiTF325693.

Family and domain databases

InterProiIPR033493. NDEL1.
IPR033494. NUDE.
IPR006964. NUDE_dom.
[Graphical view]
PANTHERiPTHR10921. PTHR10921. 1 hit.
PTHR10921:SF0. PTHR10921:SF0. 1 hit.
PfamiPF04880. NUDE_C. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q78PB6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGEDIPDFS SLKEETAYWK ELSLKYKQSF QEARDELVEF QEGSRELEAE
60 70 80 90 100
LEAQLVQAEQ RNRDLQADNQ RLKYEVEALK EKLEHQYAQS YKQVSVLEDD
110 120 130 140 150
LSQTRAIKEQ LHKYVRELEQ ANDDLERAKR ATIVSLEDFE QRLNQAIERN
160 170 180 190 200
AFLESELDEK ESLLVSVQRL KDEARDLRQE LAVRERQQEV TRKSAPSSPT
210 220 230 240 250
LDCEKMDSAV QASLSLPATP VGKGTENSFP SPKAIPNGFG TSPLTPSARI
260 270 280 290 300
SALNIVGDLL RKVGALESKL AACRNFAKDQ ASRKSYVPGS VNCGVMNSNG
310 320 330 340
PECPRSGRAT FFHKGAVNGF DPAPPPPGLG SSRPSSAPGM LPLSV
Length:345
Mass (Da):38,366
Last modified:July 5, 2004 - v1
Checksum:iCB3059DFD998E9EC
GO
Isoform 2 (identifier: Q78PB6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     316-328: AVNGFDPAPPPPG → QEKVIFPTLFMGQ
     329-345: Missing.

Note: No experimental confirmation available.
Show »
Length:328
Mass (Da):37,030
Checksum:iEDB7973DEE2DA190
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti42E → Q in AAH70909 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_019312316 – 328AVNGF…PPPPG → QEKVIFPTLFMGQ in isoform 2. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_019313329 – 345Missing in isoform 2. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY008298 mRNA. Translation: AAG21830.1.
BC070909 mRNA. Translation: AAH70909.1.
RefSeqiNP_579854.1. NM_133320.1. [Q78PB6-1]
XP_006246640.1. XM_006246578.3. [Q78PB6-2]
UniGeneiRn.2947.

Genome annotation databases

EnsembliENSRNOT00000005574; ENSRNOP00000005574; ENSRNOG00000004139. [Q78PB6-1]
ENSRNOT00000065505; ENSRNOP00000062145; ENSRNOG00000004139. [Q78PB6-2]
GeneIDi170845.
KEGGirno:170845.
UCSCiRGD:621235. rat. [Q78PB6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY008298 mRNA. Translation: AAG21830.1.
BC070909 mRNA. Translation: AAH70909.1.
RefSeqiNP_579854.1. NM_133320.1. [Q78PB6-1]
XP_006246640.1. XM_006246578.3. [Q78PB6-2]
UniGeneiRn.2947.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V71X-ray2.24A/B8-193[»]
ProteinModelPortaliQ78PB6.
SMRiQ78PB6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005574.

PTM databases

iPTMnetiQ78PB6.
PhosphoSitePlusiQ78PB6.

Proteomic databases

PaxDbiQ78PB6.
PRIDEiQ78PB6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000005574; ENSRNOP00000005574; ENSRNOG00000004139. [Q78PB6-1]
ENSRNOT00000065505; ENSRNOP00000062145; ENSRNOG00000004139. [Q78PB6-2]
GeneIDi170845.
KEGGirno:170845.
UCSCiRGD:621235. rat. [Q78PB6-1]

Organism-specific databases

CTDi81565.
RGDi621235. Ndel1.

Phylogenomic databases

eggNOGiKOG1853. Eukaryota.
ENOG410XPMP. LUCA.
GeneTreeiENSGT00390000000111.
HOGENOMiHOG000280681.
HOVERGENiHBG082010.
InParanoidiQ78PB6.
KOiK16739.
OMAiQAFLIMD.
OrthoDBiEOG091G0GBV.
PhylomeDBiQ78PB6.
TreeFamiTF325693.

Enzyme and pathway databases

ReactomeiR-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-5663220. RHO GTPases Activate Formins.
R-RNO-68877. Mitotic Prometaphase.

Miscellaneous databases

EvolutionaryTraceiQ78PB6.
PROiQ78PB6.

Gene expression databases

BgeeiENSRNOG00000004139.
GenevisibleiQ78PB6. RN.

Family and domain databases

InterProiIPR033493. NDEL1.
IPR033494. NUDE.
IPR006964. NUDE_dom.
[Graphical view]
PANTHERiPTHR10921. PTHR10921. 1 hit.
PTHR10921:SF0. PTHR10921:SF0. 1 hit.
PfamiPF04880. NUDE_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDEL1_RAT
AccessioniPrimary (citable) accession number: Q78PB6
Secondary accession number(s): Q6IRI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to function as an oligopeptidase (NUDEL-oligopeptidase or endooligopeptidase A) which could regulate peptide levels relevant to brain function.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.