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Protein

Dynein light chain 2, cytoplasmic

Gene

Dynll2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures (By similarity).By similarity

GO - Molecular functioni

  • cytoskeletal protein binding Source: RGD
  • motor activity Source: UniProtKB-KW

GO - Biological processi

  • microtubule-based process Source: InterPro
  • synaptic target recognition Source: RGD
  • transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-RNO-139910. Activation of BMF and translocation to mitochondria.
R-RNO-1632852. Macroautophagy.
R-RNO-2132295. MHC class II antigen presentation.
R-RNO-5620924. Intraflagellar transport.
R-RNO-6807878. COPI-mediated anterograde transport.
R-RNO-6811436. COPI-independent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Dynein light chain 2, cytoplasmic
Alternative name(s):
Dynein light chain LC8-type 2
Gene namesi
Name:Dynll2
Synonyms:Dlc2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi619860. Dynll2.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: Ensembl
  • cytoplasm Source: RGD
  • dynein complex Source: UniProtKB-KW
  • membrane Source: Ensembl
  • microtubule Source: UniProtKB-KW
  • myosin complex Source: Ensembl
  • nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8989Dynein light chain 2, cytoplasmicPRO_0000195134Add
BLAST

Proteomic databases

PaxDbiQ78P75.
PRIDEiQ78P75.

PTM databases

iPTMnetiQ78P75.
PhosphoSiteiQ78P75.

Expressioni

Gene expression databases

GenevisibleiQ78P75. RN.

Interactioni

Subunit structurei

Homodimer. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits which present intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. Dynein ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with DYNC1I1. Interacts with BMF and GPHN. Component of the myosin V motor complex. Interacts with rabies virus phosphoprotein.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei41 – 411Interaction with myosin V motor complexBy similarity

GO - Molecular functioni

  • cytoskeletal protein binding Source: RGD

Protein-protein interaction databases

BioGridi250846. 1 interaction.
IntActiQ78P75. 1 interaction.
MINTiMINT-4577592.
STRINGi10116.ENSRNOP00000012279.

Structurei

Secondary structure

1
89
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 115Combined sources
Helixi16 – 3116Combined sources
Helixi36 – 5015Combined sources
Beta strandi57 – 615Combined sources
Beta strandi64 – 663Combined sources
Turni67 – 693Combined sources
Beta strandi71 – 788Combined sources
Beta strandi81 – 877Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PWJNMR-A1-89[»]
1PWKNMR-A1-89[»]
ProteinModelPortaliQ78P75.
SMRiQ78P75. Positions 1-89.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ78P75.

Family & Domainsi

Sequence similaritiesi

Belongs to the dynein light chain family.Curated

Phylogenomic databases

eggNOGiKOG3430. Eukaryota.
ENOG4111NK2. LUCA.
GeneTreeiENSGT00390000000378.
HOVERGENiHBG002133.
InParanoidiQ78P75.
KOiK10418.
OMAiQIKSADM.
OrthoDBiEOG7BZVVQ.
PhylomeDBiQ78P75.
TreeFamiTF300264.

Family and domain databases

Gene3Di3.30.740.10. 1 hit.
InterProiIPR019763. Dynein_light_1/2_CS.
IPR001372. Dynein_light_chain_typ-1/2.
[Graphical view]
PANTHERiPTHR11886. PTHR11886. 1 hit.
PfamiPF01221. Dynein_light. 1 hit.
[Graphical view]
SMARTiSM01375. Dynein_light. 1 hit.
[Graphical view]
PROSITEiPS01239. DYNEIN_LIGHT_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q78P75-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDRKAVIKN ADMSEDMQQD AVDCATQAME KYNIEKDIAA YIKKEFDKKY
60 70 80
NPTWHCIVGR NFGSYVTHET KHFIYFYLGQ VAILLFKSG
Length:89
Mass (Da):10,350
Last modified:July 5, 2004 - v1
Checksum:i45364D32C0077F8E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY034383 mRNA. Translation: AAK57536.1.
BC061874 mRNA. Translation: AAH61874.1.
RefSeqiNP_542428.1. NM_080697.2.
XP_006247139.1. XM_006247077.2.
XP_008766162.1. XM_008767940.1.
UniGeneiRn.36362.

Genome annotation databases

EnsembliENSRNOT00000012279; ENSRNOP00000012279; ENSRNOG00000008921.
GeneIDi140734.
KEGGirno:140734.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY034383 mRNA. Translation: AAK57536.1.
BC061874 mRNA. Translation: AAH61874.1.
RefSeqiNP_542428.1. NM_080697.2.
XP_006247139.1. XM_006247077.2.
XP_008766162.1. XM_008767940.1.
UniGeneiRn.36362.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PWJNMR-A1-89[»]
1PWKNMR-A1-89[»]
ProteinModelPortaliQ78P75.
SMRiQ78P75. Positions 1-89.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250846. 1 interaction.
IntActiQ78P75. 1 interaction.
MINTiMINT-4577592.
STRINGi10116.ENSRNOP00000012279.

PTM databases

iPTMnetiQ78P75.
PhosphoSiteiQ78P75.

Proteomic databases

PaxDbiQ78P75.
PRIDEiQ78P75.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012279; ENSRNOP00000012279; ENSRNOG00000008921.
GeneIDi140734.
KEGGirno:140734.

Organism-specific databases

CTDi140735.
RGDi619860. Dynll2.

Phylogenomic databases

eggNOGiKOG3430. Eukaryota.
ENOG4111NK2. LUCA.
GeneTreeiENSGT00390000000378.
HOVERGENiHBG002133.
InParanoidiQ78P75.
KOiK10418.
OMAiQIKSADM.
OrthoDBiEOG7BZVVQ.
PhylomeDBiQ78P75.
TreeFamiTF300264.

Enzyme and pathway databases

ReactomeiR-RNO-139910. Activation of BMF and translocation to mitochondria.
R-RNO-1632852. Macroautophagy.
R-RNO-2132295. MHC class II antigen presentation.
R-RNO-5620924. Intraflagellar transport.
R-RNO-6807878. COPI-mediated anterograde transport.
R-RNO-6811436. COPI-independent Golgi-to-ER retrograde traffic.

Miscellaneous databases

EvolutionaryTraceiQ78P75.
PROiQ78P75.

Gene expression databases

GenevisibleiQ78P75. RN.

Family and domain databases

Gene3Di3.30.740.10. 1 hit.
InterProiIPR019763. Dynein_light_1/2_CS.
IPR001372. Dynein_light_chain_typ-1/2.
[Graphical view]
PANTHERiPTHR11886. PTHR11886. 1 hit.
PfamiPF01221. Dynein_light. 1 hit.
[Graphical view]
SMARTiSM01375. Dynein_light. 1 hit.
[Graphical view]
PROSITEiPS01239. DYNEIN_LIGHT_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gephyrin interacts with dynein light chains 1 and 2, components of motor protein complexes."
    Fuhrmann J.C., Kins S., Rostaing P., El Far O., Kirsch J., Sheng M., Triller A., Betz H., Kneussel M.
    J. Neurosci. 22:5393-5402(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, INTERACTION WITH GPHN.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  3. Lubec G., Diao W., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 32-43 AND 72-87, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain and Hippocampus.
  4. "Light chains of mammalian cytoplasmic dynein: identification and characterization of a family of LC8 light chains."
    Wilson M.J., Salata M.W., Susalka S.J., Pfister K.K.
    Cell Motil. Cytoskeleton 49:229-240(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CYTOPLASMIC DYNEIN 1 COMPLEX.
  5. "Molecular basis for the interaction between rabies virus phosphoprotein P and the dynein light chain LC8: dissociation of dynein-binding properties and transcriptional functionality of P."
    Poisson N., Real E., Gaudin Y., Vaney M.C., King S., Jacob Y., Tordo N., Blondel D.
    J. Gen. Virol. 82:2691-2696(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RABIES VIRUS PHOSPHOPROTEIN.
  6. "Structure of the monomeric 8-kDa dynein light chain and mechanism of the domain-swapped dimer assembly."
    Wang W., Lo K.-W., Kan H.-M., Fan J.-S., Zhang M.
    J. Biol. Chem. 278:41491-41499(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiDYL2_RAT
AccessioniPrimary (citable) accession number: Q78P75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: July 5, 2004
Last modified: June 8, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

As DLC8 is commonly used to refer to both DLC1 and DLC2 proteins, it is preferable to adopt the following nomenclature (see PubMed:11602781) where DLC8a and DLC8b corresponds respectively to DLC1 and DLC2.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.