ID   S22AI_MOUSE             Reviewed;         406 AA.
AC   Q78KK3; O35428; O54959; O88283; Q99NF4;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 2.
DT   24-JAN-2024, entry version 137.
DE   RecName: Full=Solute carrier family 22 member 18;
DE   AltName: Full=Imprinted multi-membrane-spanning polyspecific transporter-related protein 1;
DE            Short=Multi-membrane-spanning polyspecific transporter;
DE   AltName: Full=Organic cation transporter-like protein 2;
DE            Short=ORCTL-2;
GN   Name=Slc22a18; Synonyms=Impt1, Itm, Orctl2, Tssc5;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX   PubMed=9802569; DOI=10.1093/dnares/5.4.235;
RA   Morisaki H., Hatada I., Morisaki T., Mukai T.;
RT   "A novel gene, ITM, located between p57KIP2 and IPL, is imprinted in
RT   mice.";
RL   DNA Res. 5:235-240(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX   PubMed=9570947; DOI=10.1006/geno.1998.5221;
RA   Cooper P.R., Smilinich N.J., Day C.D., Nowak N.J., Reid L.H.,
RA   Pearsall R.S., Reece M., Prawitt D., Landers J., Housman D.E.,
RA   Winterpacht A., Zabel B.U., Pelletier J., Weissman B.E., Shows T.B.,
RA   Higgins M.J.;
RT   "Divergently transcribed overlapping genes expressed in liver and kidney
RT   and located in the 11p15.5 imprinted domain.";
RL   Genomics 49:38-51(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX   PubMed=9499412; DOI=10.1093/hmg/7.4.597;
RA   Dao D., Frank D., Qian N., O'Keefe D., Vosatka R.J., Walsh C.P., Tycko B.;
RT   "IMPT1, an imprinted gene similar to polyspecific transporter and multi-
RT   drug resistance genes.";
RL   Hum. Mol. Genet. 7:597-608(1998).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=129/Sv;
RX   PubMed=11063728; DOI=10.1093/hmg/9.18.2691;
RA   Engemann S., Stroedicke M., Paulsen M., Franck O., Reinhardt R., Lane N.,
RA   Reik W., Walter J.;
RT   "Sequence and functional comparison in the Beckwith-Wiedemann region:
RT   implications for a novel imprinting centre and extended imprinting.";
RL   Hum. Mol. Genet. 9:2691-2706(2000).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Kidney, and Liver;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: May act as a transporter of organic cations based on a proton
CC       efflux antiport mechanism. May play a role in the transport of
CC       chloroquine and quinidine-related compounds in kidney (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with RNF167. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Apical cell membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}. Note=Localized at the apical membrane
CC       surface of renal proximal tubules. {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed at high levels in fetal and adult kidney
CC       and liver, and extraembryonic membranes (yolk sac). Expressed at
CC       moderate levels in intestine, heart, lung and testis.
CC       {ECO:0000269|PubMed:9499412, ECO:0000269|PubMed:9570947,
CC       ECO:0000269|PubMed:9802569}.
CC   -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC       Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC04788.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAH03734.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AB012084; BAA32780.1; -; mRNA.
DR   EMBL; AF037065; AAC04788.1; ALT_INIT; mRNA.
DR   EMBL; AF028739; AAB82728.1; -; mRNA.
DR   EMBL; AJ276505; CAC16401.2; -; Genomic_DNA.
DR   EMBL; BC003734; AAH03734.2; ALT_INIT; mRNA.
DR   RefSeq; NP_001036225.1; NM_001042760.1.
DR   RefSeq; NP_032793.2; NM_008767.2.
DR   AlphaFoldDB; Q78KK3; -.
DR   SMR; Q78KK3; -.
DR   STRING; 10090.ENSMUSP00000101537; -.
DR   iPTMnet; Q78KK3; -.
DR   PhosphoSitePlus; Q78KK3; -.
DR   SwissPalm; Q78KK3; -.
DR   jPOST; Q78KK3; -.
DR   MaxQB; Q78KK3; -.
DR   PaxDb; 10090-ENSMUSP00000101537; -.
DR   ProteomicsDB; 260761; -.
DR   DNASU; 18400; -.
DR   GeneID; 18400; -.
DR   KEGG; mmu:18400; -.
DR   UCSC; uc009kpg.1; mouse.
DR   AGR; MGI:1336884; -.
DR   CTD; 5002; -.
DR   MGI; MGI:1336884; Slc22a18.
DR   eggNOG; ENOG502QT94; Eukaryota.
DR   InParanoid; Q78KK3; -.
DR   OrthoDB; 5320116at2759; -.
DR   PhylomeDB; Q78KK3; -.
DR   TreeFam; TF352510; -.
DR   Reactome; R-MMU-549127; Organic cation transport.
DR   BioGRID-ORCS; 18400; 4 hits in 78 CRISPR screens.
DR   ChiTaRS; Slc22a18; mouse.
DR   PRO; PR:Q78KK3; -.
DR   Proteomes; UP000000589; Unplaced.
DR   RNAct; Q78KK3; Protein.
DR   GO; GO:0016324; C:apical plasma membrane; ISO:MGI.
DR   GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR   GO; GO:0005635; C:nuclear envelope; ISO:MGI.
DR   GO; GO:0005886; C:plasma membrane; ISS:MGI.
DR   GO; GO:0008514; F:organic anion transmembrane transporter activity; TAS:MGI.
DR   GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0031625; F:ubiquitin protein ligase binding; ISO:MGI.
DR   GO; GO:0006820; P:monoatomic anion transport; TAS:MGI.
DR   GO; GO:1990961; P:xenobiotic detoxification by transmembrane export across the plasma membrane; ISO:MGI.
DR   GO; GO:0042908; P:xenobiotic transport; ISO:MGI.
DR   CDD; cd17331; MFS_SLC22A18; 1.
DR   Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR001958; Tet-R_TetA/multi-R_MdtG-like.
DR   PANTHER; PTHR24002; SOLUTE CARRIER FAMILY 22 MEMBER 18; 1.
DR   PANTHER; PTHR24002:SF3; SOLUTE CARRIER FAMILY 22 MEMBER 18; 1.
DR   Pfam; PF07690; MFS_1; 1.
DR   PRINTS; PR01035; TCRTETA.
DR   SUPFAM; SSF103473; MFS general substrate transporter; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Ion transport; Membrane; Reference proteome; Symport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..406
FT                   /note="Solute carrier family 22 member 18"
FT                   /id="PRO_0000220510"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        43..63
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        140..160
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        168..188
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        226..246
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        258..278
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        295..315
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        316..336
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        374..394
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        33
FT                   /note="Y -> V (in Ref. 4; CAC16401)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        66
FT                   /note="F -> Y (in Ref. 4; CAC16401)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        68
FT                   /note="D -> N (in Ref. 2; AAC04788)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        83
FT                   /note="A -> G (in Ref. 3; AAB82728)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        117
FT                   /note="L -> I (in Ref. 2; AAC04788)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        119..120
FT                   /note="AA -> GT (in Ref. 4; CAC16401)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        149
FT                   /note="I -> M (in Ref. 2; AAC04788)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        164
FT                   /note="I -> Y (in Ref. 4; CAC16401)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        178
FT                   /note="G -> R (in Ref. 3; AAB82728)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        205
FT                   /note="A -> G (in Ref. 3; AAB82728)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        228..231
FT                   /note="VKVI -> SVF (in Ref. 2; AAC04788)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        238
FT                   /note="L -> V (in Ref. 4; CAC16401)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        271
FT                   /note="M -> V (in Ref. 4; CAC16401)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        305
FT                   /note="A -> V (in Ref. 4; CAC16401)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        346
FT                   /note="T -> A (in Ref. 4; CAC16401)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   406 AA;  43013 MW;  034D8223CEB8013C CRC64;
     MAALPRQGII ILTYVLAALE LTCLFMQFSI LPYLSRTLGL DSVSFGYLQT TFGVLQLLGG
     PVFGRFADQC GARAALSLSF LAASALYLLL VASCSPALPG VFLLFASRIP SALMHTLPAA
     QMVITDLTAP TERPAALGRL GLCFGIGIIF GSLLGGTLNT AYGIQCPAIL AFVVTLLGAV
     LSFTCVPATT KEASVQSAPQ GGTKASVFDL KAITRLLLLP RVLPVFLVKV ISGLPSGLFL
     VMFSIISMDF FQLEAAQAGY LMSFFGILQM MIQGLVIGRL STHFPEEALL RSSVLVFAVV
     GLGMALMSSV LHFCFLMPGL VFSLCTLNVV TDSMLTKAVS ASDTGTMLGL SASVQPLTRT
     LGPTLGGLLY RSYGVPIFGH VQLMVNLLVL LVLWKKPLSQ KGEKAR
//