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Protein

3-hydroxyanthranilate 3,4-dioxygenase

Gene

Haao

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.UniRule annotation

Catalytic activityi

3-hydroxyanthranilate + O2 = 2-amino-3-carboxymuconate semialdehyde.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes quinolinate from L-kynurenine.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Kynurenine 3-monooxygenase (Kmo)
  2. Kynureninase (Kynu), Kynureninase (Kynu), Kynureninase (Kynu)
  3. 3-hydroxyanthranilate 3,4-dioxygenase (Haao)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from L-kynurenine, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43DioxygenUniRule annotation1
Metal bindingi47Iron; catalyticUniRule annotation1
Metal bindingi53Iron; catalyticUniRule annotation1
Binding sitei53SubstrateUniRule annotation1
Metal bindingi91Iron; catalyticUniRule annotation1
Binding sitei95SubstrateUniRule annotation1
Binding sitei105SubstrateUniRule annotation1

GO - Molecular functioni

  • 3-hydroxyanthranilate 3,4-dioxygenase activity Source: MGI
  • ferrous iron binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processPyridine nucleotide biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-71240 Tryptophan catabolism
UniPathwayiUPA00253; UER00330

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxyanthranilate 3,4-dioxygenaseUniRule annotation (EC:1.13.11.6UniRule annotation)
Alternative name(s):
3-hydroxyanthranilate oxygenaseUniRule annotation
Short name:
3-HAOUniRule annotation
3-hydroxyanthranilic acid dioxygenaseUniRule annotation
Short name:
HADUniRule annotation
Gene namesi
Name:Haao
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:1349444 Haao

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Mice were born at the expected Mendelian ratio and are normal. They however show very high levels of 3-hydroxyanthranilate compared to wild-type mice.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000643731 – 2863-hydroxyanthranilate 3,4-dioxygenaseAdd BLAST286

Proteomic databases

EPDiQ78JT3
MaxQBiQ78JT3
PaxDbiQ78JT3
PeptideAtlasiQ78JT3
PRIDEiQ78JT3

PTM databases

iPTMnetiQ78JT3
PhosphoSitePlusiQ78JT3

Expressioni

Gene expression databases

BgeeiENSMUSG00000000673
CleanExiMM_HAAO
GenevisibleiQ78JT3 MM

Interactioni

Subunit structurei

Monomer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000000687

Structurei

3D structure databases

ProteinModelPortaliQ78JT3
SMRiQ78JT3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 160Domain A (catalytic)UniRule annotationAdd BLAST160
Regioni161 – 177LinkerUniRule annotationAdd BLAST17
Regioni178 – 286Domain BUniRule annotationAdd BLAST109

Sequence similaritiesi

Belongs to the 3-HAO family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3995 Eukaryota
ENOG4111GH8 LUCA
GeneTreeiENSGT00390000013008
HOGENOMiHOG000218448
HOVERGENiHBG000018
InParanoidiQ78JT3
KOiK00452
OMAiCKDLGTQ
OrthoDBiEOG091G0IJ1
PhylomeDBiQ78JT3
TreeFamiTF300246

Family and domain databases

Gene3Di2.60.120.10, 1 hit
HAMAPiMF_00825 3_HAO, 1 hit
InterProiView protein in InterPro
IPR010329 3hydroanth_dOase
IPR016700 3hydroanth_dOase_met
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PANTHERiPTHR15497 PTHR15497, 1 hit
PfamiView protein in Pfam
PF06052 3-HAO, 1 hit
PIRSFiPIRSF017681 3hydroanth_dOase_animal, 1 hit
SUPFAMiSSF51182 SSF51182, 2 hits
TIGRFAMsiTIGR03037 anthran_nbaC, 1 hit

Sequencei

Sequence statusi: Complete.

Q78JT3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERRVRVKSW VEENRASFQP PVCNKLMHQE QLKIMFVGGP NTRKDYHIEE
60 70 80 90 100
GEEVFYQLEG DMILRVLEQG QHRDVPIRQG EIFLLPARVP HSPQRFANTM
110 120 130 140 150
GLVIERRRLE SELDGLRYYV GDTEDVLFEK WFHCKDLGTQ LAPIIQEFFH
160 170 180 190 200
SEQYRTGKPN PDQLLKELPF PLNTRSIMKP MSLKAWLDGH SRELQAGTSL
210 220 230 240 250
SLFGDSYETQ VIAHGQGSSK GPRQDVDVWL WQQEGSSKVT MGGQCIALAP
260 270 280
DDSLLVPAGT SYVWERAQGS VALSVTQDPA RKKPWW
Length:286
Mass (Da):32,804
Last modified:July 5, 2004 - v1
Checksum:iCD8A706EE264B4CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC012872 mRNA Translation: AAH12872.1
BC094021 mRNA Translation: AAH94021.1
CCDSiCCDS37709.1
RefSeqiNP_079601.1, NM_025325.2
XP_006523526.1, XM_006523463.3
UniGeneiMm.30100

Genome annotation databases

EnsembliENSMUST00000000687; ENSMUSP00000000687; ENSMUSG00000000673
GeneIDi107766
KEGGimmu:107766
UCSCiuc008dsk.1 mouse

Entry informationi

Entry namei3HAO_MOUSE
AccessioniPrimary (citable) accession number: Q78JT3
Secondary accession number(s): Q52L88
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: July 5, 2004
Last modified: April 25, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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