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Protein

Methionine-R-sulfoxide reductase B2, mitochondrial

Gene

Msrb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Methionine-sulfoxide reductase that specifically reduces methionine (R)-sulfoxide back to methionine. While in many cases, methionine oxidation is the result of random oxidation following oxidative stress, methionine oxidation is also a post-translational modification that takes place on specific residue. Upon oxidative stress, may play a role in the preservation of mitochondrial integrity by decreasing the intracellular reactive oxygen species build-up through its scavenging role, hence contributing to cell survival and protein maintenance.2 Publications

Catalytic activityi

L-methionine + oxidized thioredoxin = L-methionine R-oxide + reduced thioredoxin.

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Enzyme regulationi

Inhibited by high concentrations of substrate.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi83 – 831ZincPROSITE-ProRule annotation
Metal bindingi86 – 861ZincPROSITE-ProRule annotation
Metal bindingi139 – 1391ZincPROSITE-ProRule annotation
Metal bindingi142 – 1421ZincPROSITE-ProRule annotation
Active sitei162 – 1621NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • peptide-methionine (R)-S-oxide reductase activity Source: UniProtKB
  • zinc ion binding Source: HGNC

GO - Biological processi

  • actin filament polymerization Source: UniProtKB
  • protein repair Source: HGNC
  • response to oxidative stress Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi1.8.4.12. 3474.
ReactomeiR-MMU-5676934. Protein repair.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine-R-sulfoxide reductase B2, mitochondrial (EC:1.8.4.-)
Short name:
MsrB2
Gene namesi
Name:Msrb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1923717. Msrb2.

Subcellular locationi

  • Mitochondrion 1 Publication

GO - Cellular componenti

  • mitochondrion Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4242MitochondrionSequence analysisAdd
BLAST
Chaini43 – 175133Methionine-R-sulfoxide reductase B2, mitochondrialPRO_0000327243Add
BLAST

Proteomic databases

MaxQBiQ78J03.
PaxDbiQ78J03.
PRIDEiQ78J03.

PTM databases

PhosphoSiteiQ78J03.
SwissPalmiQ78J03.

Expressioni

Gene expression databases

BgeeiQ78J03.
GenevisibleiQ78J03. MM.

Interactioni

GO - Molecular functioni

  • actin binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ78J03. 1 interaction.
STRINGi10090.ENSMUSP00000023856.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi48 – 514Combined sources
Helixi55 – 617Combined sources
Helixi70 – 723Combined sources
Beta strandi79 – 879Combined sources
Beta strandi89 – 924Combined sources
Helixi93 – 953Combined sources
Turni98 – 1014Combined sources
Beta strandi104 – 1085Combined sources
Helixi116 – 1194Combined sources
Beta strandi122 – 1276Combined sources
Beta strandi134 – 1429Combined sources
Beta strandi147 – 1504Combined sources
Helixi156 – 1583Combined sources
Beta strandi160 – 1634Combined sources
Beta strandi168 – 1758Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L1UNMR-A45-175[»]
ProteinModelPortaliQ78J03.
SMRiQ78J03. Positions 45-175.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 173130MsrBPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 MsrB (methionine-R-sulfoxide reductase) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0856. Eukaryota.
COG0229. LUCA.
GeneTreeiENSGT00510000046802.
HOGENOMiHOG000243424.
HOVERGENiHBG002192.
InParanoidiQ78J03.
KOiK07305.
OMAiFVHRDDM.
OrthoDBiEOG7VHSZD.
PhylomeDBiQ78J03.
TreeFamiTF329147.

Family and domain databases

Gene3Di2.170.150.20. 1 hit.
InterProiIPR028427. Met_Sox_Rdtase.
IPR002579. Met_Sox_Rdtase_MsrB.
IPR011057. Mss4-like.
[Graphical view]
PANTHERiPTHR10173. PTHR10173. 1 hit.
PfamiPF01641. SelR. 1 hit.
[Graphical view]
SUPFAMiSSF51316. SSF51316. 1 hit.
TIGRFAMsiTIGR00357. TIGR00357. 1 hit.
PROSITEiPS51790. MSRB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q78J03-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARLLRALRG LPLLQAPGRL ARGCAGSGSK DTGSLTKSKR SLSEADWQKK
60 70 80 90 100
LTPEQFYVTR EKGTEAPFSG MYLNNKETGM YHCVCCDSPL FSSEKKYCSG
110 120 130 140 150
TGWPSFSEAY GSKGSDESHT GILRRLDTSL GCPRMEVVCK QCEAHLGHVF
160 170
PDGPKPTGQR FCINSVALKF KPSKP
Length:175
Mass (Da):19,157
Last modified:July 5, 2004 - v1
Checksum:i916771737762C6A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL928806 Genomic DNA. Translation: CAM21786.1.
BC021619 mRNA. Translation: AAH21619.1.
CCDSiCCDS15714.1.
RefSeqiNP_083895.1. NM_029619.2.
UniGeneiMm.27166.

Genome annotation databases

EnsembliENSMUST00000023856; ENSMUSP00000023856; ENSMUSG00000023094.
GeneIDi76467.
KEGGimmu:76467.
UCSCiuc008imf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL928806 Genomic DNA. Translation: CAM21786.1.
BC021619 mRNA. Translation: AAH21619.1.
CCDSiCCDS15714.1.
RefSeqiNP_083895.1. NM_029619.2.
UniGeneiMm.27166.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L1UNMR-A45-175[»]
ProteinModelPortaliQ78J03.
SMRiQ78J03. Positions 45-175.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ78J03. 1 interaction.
STRINGi10090.ENSMUSP00000023856.

PTM databases

PhosphoSiteiQ78J03.
SwissPalmiQ78J03.

Proteomic databases

MaxQBiQ78J03.
PaxDbiQ78J03.
PRIDEiQ78J03.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023856; ENSMUSP00000023856; ENSMUSG00000023094.
GeneIDi76467.
KEGGimmu:76467.
UCSCiuc008imf.2. mouse.

Organism-specific databases

CTDi22921.
MGIiMGI:1923717. Msrb2.

Phylogenomic databases

eggNOGiKOG0856. Eukaryota.
COG0229. LUCA.
GeneTreeiENSGT00510000046802.
HOGENOMiHOG000243424.
HOVERGENiHBG002192.
InParanoidiQ78J03.
KOiK07305.
OMAiFVHRDDM.
OrthoDBiEOG7VHSZD.
PhylomeDBiQ78J03.
TreeFamiTF329147.

Enzyme and pathway databases

BRENDAi1.8.4.12. 3474.
ReactomeiR-MMU-5676934. Protein repair.

Miscellaneous databases

NextBioi345204.
PROiQ78J03.
SOURCEiSearch...

Gene expression databases

BgeeiQ78J03.
GenevisibleiQ78J03. MM.

Family and domain databases

Gene3Di2.170.150.20. 1 hit.
InterProiIPR028427. Met_Sox_Rdtase.
IPR002579. Met_Sox_Rdtase_MsrB.
IPR011057. Mss4-like.
[Graphical view]
PANTHERiPTHR10173. PTHR10173. 1 hit.
PfamiPF01641. SelR. 1 hit.
[Graphical view]
SUPFAMiSSF51316. SSF51316. 1 hit.
TIGRFAMsiTIGR00357. TIGR00357. 1 hit.
PROSITEiPS51790. MSRB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "Methionine sulfoxide reduction in mammals: characterization of methionine-R-sulfoxide reductases."
    Kim H.-Y., Gladyshev V.N.
    Mol. Biol. Cell 15:1055-1064(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, ENZYME REGULATION, COFACTOR.
  4. "MsrB1 and MICALs regulate actin assembly and macrophage function via reversible stereoselective methionine oxidation."
    Lee B.C., Peterfi Z., Hoffmann F.W., Moore R.E., Kaya A., Avanesov A., Tarrago L., Zhou Y., Weerapana E., Fomenko D.E., Hoffmann P.R., Gladyshev V.N.
    Mol. Cell 51:397-404(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMSRB2_MOUSE
AccessioniPrimary (citable) accession number: Q78J03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: July 5, 2004
Last modified: April 13, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.