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Protein

Transmembrane emp24 domain-containing protein 3

Gene

Tmed3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Potential role in vesicular protein trafficking, mainly in the early secretory pathway. Contributes to the coupled localization of TMED2 and TMED10 in the cis-Golgi network (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-6807878. COPI-mediated anterograde transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane emp24 domain-containing protein 3
Alternative name(s):
p24 family protein gamma-4
Short name:
p24gamma4
Gene namesi
Name:Tmed3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1913361. Tmed3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 184157LumenalSequence analysisAdd
BLAST
Transmembranei185 – 20521HelicalSequence analysisAdd
BLAST
Topological domaini206 – 22116CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 221196Transmembrane emp24 domain-containing protein 3PRO_0000010385Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei103 – 1031Dimethylated arginine; alternateBy similarity
Modified residuei103 – 1031Omega-N-methylated arginine; alternateBy similarity

Keywords - PTMi

Methylation

Proteomic databases

EPDiQ78IS1.
MaxQBiQ78IS1.
PaxDbiQ78IS1.
PRIDEiQ78IS1.

PTM databases

PhosphoSiteiQ78IS1.

Expressioni

Gene expression databases

BgeeiQ78IS1.
CleanExiMM_TMED3.
GenevisibleiQ78IS1. MM.

Interactioni

Subunit structurei

Monomer in endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi network. Interacts (via C-terminus) with COPG1; the interaction involves dimeric TMED3; however, there are conflicting reports on the interaction. Interacts with GORASP1 and GORASP2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000058723.

Structurei

3D structure databases

ProteinModelPortaliQ78IS1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 12483GOLDPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi208 – 22114COPI vesicle coat-bindingSequence analysisAdd
BLAST
Motifi208 – 2092COPII vesicle coat-bindingSequence analysis

Sequence similaritiesi

Belongs to the EMP24/GP25L family.Curated
Contains 1 GOLD domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1693. Eukaryota.
ENOG4111JPD. LUCA.
GeneTreeiENSGT00670000097656.
HOGENOMiHOG000160229.
HOVERGENiHBG000271.
InParanoidiQ78IS1.
OMAiGRVSYWS.
OrthoDBiEOG70ZZP7.
PhylomeDBiQ78IS1.
TreeFamiTF313000.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
SUPFAMiSSF101576. SSF101576. 1 hit.
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q78IS1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHEAPHASS FQMLLQLLLL LLLRAEPLRS AELTFELPDN AKQCFHEEVE
60 70 80 90 100
QGVKFSLDYQ VITGGHYDVD CYVEDPRGNV IYRETKKQYD SFTYKTEAKG
110 120 130 140 150
VYRFCFSNEF STFSHKTVYF DFQVGDEPPI LPDMGNRVTA LTQMESACVT
160 170 180 190 200
IHEALKTVID SQTHYRLREA QDRARAEDLN SRVSYWSVGE TIALFVVSFS
210 220
QVLLLKSFFT EKRPVNRAVH S
Length:221
Mass (Da):25,465
Last modified:July 5, 2004 - v1
Checksum:iA1DA79631DAFB8DC
GO

Sequence cautioni

The sequence BAB22292.1 differs from that shown. Reason: Frameshift at positions 13 and 30. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 181L → A in BAB23361 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002698 mRNA. Translation: BAB22292.1. Frameshift.
AK004545 mRNA. Translation: BAB23361.1.
BC023338 mRNA. Translation: AAH23338.1.
CCDSiCCDS23396.1.
RefSeqiNP_079636.2. NM_025360.2.
UniGeneiMm.27606.

Genome annotation databases

EnsembliENSMUST00000058488; ENSMUSP00000058723; ENSMUSG00000032353.
GeneIDi66111.
KEGGimmu:66111.
UCSCiuc009qzo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002698 mRNA. Translation: BAB22292.1. Frameshift.
AK004545 mRNA. Translation: BAB23361.1.
BC023338 mRNA. Translation: AAH23338.1.
CCDSiCCDS23396.1.
RefSeqiNP_079636.2. NM_025360.2.
UniGeneiMm.27606.

3D structure databases

ProteinModelPortaliQ78IS1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000058723.

PTM databases

PhosphoSiteiQ78IS1.

Proteomic databases

EPDiQ78IS1.
MaxQBiQ78IS1.
PaxDbiQ78IS1.
PRIDEiQ78IS1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058488; ENSMUSP00000058723; ENSMUSG00000032353.
GeneIDi66111.
KEGGimmu:66111.
UCSCiuc009qzo.2. mouse.

Organism-specific databases

CTDi23423.
MGIiMGI:1913361. Tmed3.

Phylogenomic databases

eggNOGiKOG1693. Eukaryota.
ENOG4111JPD. LUCA.
GeneTreeiENSGT00670000097656.
HOGENOMiHOG000160229.
HOVERGENiHBG000271.
InParanoidiQ78IS1.
OMAiGRVSYWS.
OrthoDBiEOG70ZZP7.
PhylomeDBiQ78IS1.
TreeFamiTF313000.

Enzyme and pathway databases

ReactomeiR-MMU-6807878. COPI-mediated anterograde transport.

Miscellaneous databases

NextBioi320658.
PROiQ78IS1.
SOURCEiSearch...

Gene expression databases

BgeeiQ78IS1.
CleanExiMM_TMED3.
GenevisibleiQ78IS1. MM.

Family and domain databases

InterProiIPR009038. GOLD_dom.
IPR015720. TMP21-related.
[Graphical view]
PANTHERiPTHR22811. PTHR22811. 1 hit.
PfamiPF01105. EMP24_GP25L. 1 hit.
[Graphical view]
SMARTiSM01190. EMP24_GP25L. 1 hit.
[Graphical view]
SUPFAMiSSF101576. SSF101576. 1 hit.
PROSITEiPS50866. GOLD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Kidney and Lung.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiTMED3_MOUSE
AccessioniPrimary (citable) accession number: Q78IS1
Secondary accession number(s): Q9DC62, Q9DCK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.