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Q78EJ9 (CAN8_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Calpain-8
EC=3.4.22.53
Alternative name(s):
Calpain large subunit 4
New calpain 2
Short name=nCL-2
Stomach-specific M-type calpain
Gene names
Name:Capn8
Synonyms:Cls4, Ncl2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length703 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Calcium-regulated non-lysosomal thiol-protease. Involved in membrane trafficking in the gastric surface mucus cells (pit cells) and may involve the membrane trafficking of mucus cells via interactions with coat protein. Proteolytically cleaves the beta-subunit of coatomer complex By similarity.

Catalytic activity

Broad endopeptidase specificity.

Cofactor

Binds 2 calcium ions Potential.

Subunit structure

Monomer and homooligomer. Interacts with COPS1/GPS1, COPB1, EYA2, NME2, NME4 and TOMM70A By similarity.

Subcellular location

Cytoplasm. Golgi apparatus By similarity Ref.1.

Tissue specificity

Predominantly expressed in the stomach. Localizes strictly to the surface mucus cells in the gastric epithelium and the mucus-secreting goblet cells in the duodenum. Detected in the pituitary after estrogen stimulation. Ref.1 Ref.2

Domain

The domain III mediates oligomerization By similarity.

Post-translational modification

Undergoes autolytic cleavage between Ala-5 and Ala-6 which gives rise to fragments extending from Ala-6 to the C-terminus, Ala-6 to the EF-hand 2 domain and from Ala-6 to the beginning of domain III By similarity.

Sequence similarities

Belongs to the peptidase C2 family.

Contains 1 calpain catalytic domain.

Contains 4 EF-hand domains.

Ontologies

Keywords
   Cellular componentCytoplasm
Golgi apparatus
   Coding sequence diversityAlternative splicing
   DomainRepeat
   LigandCalcium
Metal-binding
   Molecular functionHydrolase
Protease
Thiol protease
   PTMAutocatalytic cleavage
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

calcium-dependent cysteine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q78EJ9-1)

Also known as: nCL-2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q78EJ9-2)

Also known as: Calpain 8b; nCL-2';

The sequence of this isoform differs from the canonical sequence as follows:
     380-381: TY → SS
     382-703: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 703703Calpain-8
PRO_0000349282

Regions

Domain45 – 344300Calpain catalytic
Domain532 – 56635EF-hand 1
Domain575 – 60834EF-hand 2
Domain605 – 64036EF-hand 3
Domain670 – 70334EF-hand 4
Calcium binding588 – 599121 Potential
Calcium binding618 – 629122 Potential
Region355 – 512158Domain III
Region513 – 53119Linker By similarity
Region532 – 703172Domain IV By similarity

Sites

Active site1051 By similarity
Active site2621 By similarity
Active site2861 By similarity

Natural variations

Alternative sequence380 – 3812TY → SS in isoform 2.
VSP_035308
Alternative sequence382 – 703322Missing in isoform 2.
VSP_035309

Experimental info

Sequence conflict1031G → V in BAA03369. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (nCL-2) [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: C0688B055FC0D6EC

FASTA70379,555
        10         20         30         40         50         60 
MAALAAGVSK QRAVAEGLGS NQNAVKYLGQ DFETLRKQCL NSGVLFKDPE FPACPSALGY 

        70         80         90        100        110        120 
KDLGPGSPDT QGIVWKRPTE LCPNPQFIVG GATRTDIRQG GLGDCWLLAA IASLTLNEKL 

       130        140        150        160        170        180 
LYRVLPRDQS FQKDYAGIFH FQFWQYGEWV EVVIDDRLPT KNGQLLFLHS EEGNEFWSAL 

       190        200        210        220        230        240 
LEKAYAKLNG SYEALVGGST IEGFEDFTGG ISEFYDLKKP PENLYYIIQK ALRKGSLLGC 

       250        260        270        280        290        300 
SIDVSTAAEA EATTRQKLVK GHAYSVTGVE EVNFHGRPEK LIRLRNPWGE VEWSGAWSDN 

       310        320        330        340        350        360 
APEWNYIDPR RKEELDKKAE DGEFWMSFSD FLKQYSRLEI CNLSPDSLSS EEIHKWNLVL 

       370        380        390        400        410        420 
FNGRWTRGST AGGCLNYPGT YWTNPQFKIH LDEVDEDQEE GTSEPCCTVL LGLMQKNRRR 

       430        440        450        460        470        480 
QKRIGQGMLS IGYAVYQIPK ELESHTDAHL GRDFFLGRQP STCSSTYMNL REVSSRVRLP 

       490        500        510        520        530        540 
PGQYLVVPST FEPFKDGDFC LRVFSEKKAK ALEIGDTVSG HPHEPHPRDM DEEDEHVRSL 

       550        560        570        580        590        600 
FEEFVGKDSE ISANQLKRVL NEVLSKRTDM KFDGFNINTC REMISLLDSD GTGSLGPMEF 

       610        620        630        640        650        660 
KTLWLKIRTY LEIFQEMDHN HVGTIEAHEM RTALKKAGFT LNNQVQQTIA MRYACSKLGV 

       670        680        690        700 
DFNGFVACMI RLETLFKLFR LLDKDQNGIV QLSLAEWLCC VLV

« Hide

Isoform 2 (Calpain 8b) (nCL-2') [UniParc].

Checksum: E738BAFF3EC827E1
Show »

FASTA38142,592

References

[1]"A novel tissue-specific calpain species expressed predominantly in the stomach comprises two alternative splicing products with and without Ca(2+)-binding domain."
Sorimachi H., Ishiura S., Suzuki K.
J. Biol. Chem. 268:19476-19482(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Gastric mucosa.
[2]"Estrogen regulates a tissue-specific calpain in the anterior pituitary."
Duan W.R., Ito M., Lee E.J., Chien P.-Y., Jameson J.L.
Biochem. Biophys. Res. Commun. 295:261-266(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-17, TISSUE SPECIFICITY.
Strain: Sprague-Dawley.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D14478 Transcribed RNA. Translation: BAA03369.1.
D14479 mRNA. Translation: BAA03370.1.
D14480 mRNA. Translation: BAA03371.1.
AF514419 Genomic DNA. Translation: AAM94284.1.
IPIIPI00421680.
IPI00421823.
PIRA48764.
RefSeqNP_579843.2. NM_133309.2.
UniGeneRn.80837.

3D structure databases

ProteinModelPortalQ78EJ9.
SMRQ78EJ9. Positions 2-699.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000062898.

Protein family/group databases

MEROPSC02.007.

Proteomic databases

PaxDbQ78EJ9.
PRIDEQ78EJ9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID170808.
KEGGrno:170808.
UCSCRGD:620085. rat.

Organism-specific databases

CTD388743.
RGD620085. Capn8.

Phylogenomic databases

eggNOGNOG327523.
HOGENOMHOG000232035.
HOVERGENHBG012645.
InParanoidQ64698.
KOK08577.
OMAQTIAMRY.
OrthoDBEOG4HX50K.

Gene expression databases

ArrayExpressQ78EJ9.
GenevestigatorQ78EJ9.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-like_dom.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamPF01067. Calpain_III. 1 hit.
PF13405. EF_hand_4. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSPR00704. CALPAIN.
SMARTSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMSSF49758. Peptidase_C2. 1 hit.
PROSITEPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 4 hits.
PS00640. THIOL_PROTEASE_ASN. False negative.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio621219.

Entry information

Entry nameCAN8_RAT
AccessionPrimary (citable) accession number: Q78EJ9
Secondary accession number(s): Q64698, Q78EJ8, Q8K407
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: July 5, 2004
Last modified: May 29, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

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