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Protein

Calpain-8

Gene

Capn8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease. Involved in membrane trafficking in the gastric surface mucus cells (pit cells) and may involve the membrane trafficking of mucus cells via interactions with coat protein. Proteolytically cleaves the beta-subunit of coatomer complex (By similarity).By similarity

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Ca2+CuratedNote: Binds 2 calcium ions.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei105By similarity1
Active sitei262By similarity1
Active sitei286By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi588 – 5991PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi618 – 6292PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiC02.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-8 (EC:3.4.22.53)
Alternative name(s):
Calpain large subunit 4
New calpain 2
Short name:
nCL-2
Stomach-specific M-type calpain
Gene namesi
Name:Capn8
Synonyms:Cls4, Ncl2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi620085. Capn8.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Golgi apparatus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003492821 – 703Calpain-8Add BLAST703

Post-translational modificationi

Undergoes autolytic cleavage between Ala-5 and Ala-6 which gives rise to fragments extending from Ala-6 to the C-terminus, Ala-6 to the EF-hand 2 domain and from Ala-6 to the beginning of domain III.By similarity

Keywords - PTMi

Autocatalytic cleavage

Proteomic databases

PaxDbiQ78EJ9.
PRIDEiQ78EJ9.

PTM databases

iPTMnetiQ78EJ9.
PhosphoSitePlusiQ78EJ9.

Expressioni

Tissue specificityi

Predominantly expressed in the stomach. Localizes strictly to the surface mucus cells in the gastric epithelium and the mucus-secreting goblet cells in the duodenum. Detected in the pituitary after estrogen stimulation.2 Publications

Gene expression databases

ExpressionAtlasiQ78EJ9. baseline and differential.
GenevisibleiQ78EJ9. RN.

Interactioni

Subunit structurei

Monomer and homooligomer. Interacts with COPS1/GPS1, COPB1, EYA2, NME2, NME4 and TOMM70 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000062898.

Structurei

3D structure databases

ProteinModelPortaliQ78EJ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 344Calpain catalyticPROSITE-ProRule annotationAdd BLAST300
Domaini532 – 566EF-hand 1PROSITE-ProRule annotationAdd BLAST35
Domaini575 – 608EF-hand 2PROSITE-ProRule annotationAdd BLAST34
Domaini605 – 640EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini670 – 703EF-hand 4PROSITE-ProRule annotationAdd BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni355 – 512Domain IIIAdd BLAST158
Regioni513 – 531LinkerBy similarityAdd BLAST19
Regioni532 – 703Domain IVBy similarityAdd BLAST172

Domaini

The domain III mediates oligomerization.By similarity

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ78EJ9.
KOiK08577.
OMAiQTIAMRY.
OrthoDBiEOG091G049E.
PhylomeDBiQ78EJ9.
TreeFamiTF314748.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029543. CAPN8.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF52. PTHR10183:SF52. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q78EJ9-1) [UniParc]FASTAAdd to basket
Also known as: nCL-2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAALAAGVSK QRAVAEGLGS NQNAVKYLGQ DFETLRKQCL NSGVLFKDPE
60 70 80 90 100
FPACPSALGY KDLGPGSPDT QGIVWKRPTE LCPNPQFIVG GATRTDIRQG
110 120 130 140 150
GLGDCWLLAA IASLTLNEKL LYRVLPRDQS FQKDYAGIFH FQFWQYGEWV
160 170 180 190 200
EVVIDDRLPT KNGQLLFLHS EEGNEFWSAL LEKAYAKLNG SYEALVGGST
210 220 230 240 250
IEGFEDFTGG ISEFYDLKKP PENLYYIIQK ALRKGSLLGC SIDVSTAAEA
260 270 280 290 300
EATTRQKLVK GHAYSVTGVE EVNFHGRPEK LIRLRNPWGE VEWSGAWSDN
310 320 330 340 350
APEWNYIDPR RKEELDKKAE DGEFWMSFSD FLKQYSRLEI CNLSPDSLSS
360 370 380 390 400
EEIHKWNLVL FNGRWTRGST AGGCLNYPGT YWTNPQFKIH LDEVDEDQEE
410 420 430 440 450
GTSEPCCTVL LGLMQKNRRR QKRIGQGMLS IGYAVYQIPK ELESHTDAHL
460 470 480 490 500
GRDFFLGRQP STCSSTYMNL REVSSRVRLP PGQYLVVPST FEPFKDGDFC
510 520 530 540 550
LRVFSEKKAK ALEIGDTVSG HPHEPHPRDM DEEDEHVRSL FEEFVGKDSE
560 570 580 590 600
ISANQLKRVL NEVLSKRTDM KFDGFNINTC REMISLLDSD GTGSLGPMEF
610 620 630 640 650
KTLWLKIRTY LEIFQEMDHN HVGTIEAHEM RTALKKAGFT LNNQVQQTIA
660 670 680 690 700
MRYACSKLGV DFNGFVACMI RLETLFKLFR LLDKDQNGIV QLSLAEWLCC

VLV
Length:703
Mass (Da):79,555
Last modified:July 5, 2004 - v1
Checksum:iC0688B055FC0D6EC
GO
Isoform 2 (identifier: Q78EJ9-2) [UniParc]FASTAAdd to basket
Also known as: Calpain 8b, nCL-2'

The sequence of this isoform differs from the canonical sequence as follows:
     380-381: TY → SS
     382-703: Missing.

Show »
Length:381
Mass (Da):42,592
Checksum:iE738BAFF3EC827E1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti103G → V in BAA03369 (PubMed:7690035).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_035308380 – 381TY → SS in isoform 2. 1 Publication2
Alternative sequenceiVSP_035309382 – 703Missing in isoform 2. 1 PublicationAdd BLAST322

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14478 Transcribed RNA. Translation: BAA03369.1.
D14479 mRNA. Translation: BAA03370.1.
D14480 mRNA. Translation: BAA03371.1.
AF514419 Genomic DNA. Translation: AAM94284.1.
PIRiA48764.
RefSeqiNP_579843.2. NM_133309.2. [Q78EJ9-1]
UniGeneiRn.80837.

Genome annotation databases

EnsembliENSRNOT00000004649; ENSRNOP00000004649; ENSRNOG00000003468. [Q78EJ9-2]
ENSRNOT00000067005; ENSRNOP00000062898; ENSRNOG00000003468. [Q78EJ9-1]
GeneIDi170808.
KEGGirno:170808.
UCSCiRGD:620085. rat. [Q78EJ9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14478 Transcribed RNA. Translation: BAA03369.1.
D14479 mRNA. Translation: BAA03370.1.
D14480 mRNA. Translation: BAA03371.1.
AF514419 Genomic DNA. Translation: AAM94284.1.
PIRiA48764.
RefSeqiNP_579843.2. NM_133309.2. [Q78EJ9-1]
UniGeneiRn.80837.

3D structure databases

ProteinModelPortaliQ78EJ9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000062898.

Protein family/group databases

MEROPSiC02.007.

PTM databases

iPTMnetiQ78EJ9.
PhosphoSitePlusiQ78EJ9.

Proteomic databases

PaxDbiQ78EJ9.
PRIDEiQ78EJ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004649; ENSRNOP00000004649; ENSRNOG00000003468. [Q78EJ9-2]
ENSRNOT00000067005; ENSRNOP00000062898; ENSRNOG00000003468. [Q78EJ9-1]
GeneIDi170808.
KEGGirno:170808.
UCSCiRGD:620085. rat. [Q78EJ9-1]

Organism-specific databases

CTDi388743.
RGDi620085. Capn8.

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ78EJ9.
KOiK08577.
OMAiQTIAMRY.
OrthoDBiEOG091G049E.
PhylomeDBiQ78EJ9.
TreeFamiTF314748.

Miscellaneous databases

PROiQ78EJ9.

Gene expression databases

ExpressionAtlasiQ78EJ9. baseline and differential.
GenevisibleiQ78EJ9. RN.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029543. CAPN8.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF52. PTHR10183:SF52. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAN8_RAT
AccessioniPrimary (citable) accession number: Q78EJ9
Secondary accession number(s): Q64698, Q78EJ8, Q8K407
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.