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Q78EJ9

- CAN8_RAT

UniProt

Q78EJ9 - CAN8_RAT

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Protein
Calpain-8
Gene
Capn8, Cls4, Ncl2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease. Involved in membrane trafficking in the gastric surface mucus cells (pit cells) and may involve the membrane trafficking of mucus cells via interactions with coat protein. Proteolytically cleaves the beta-subunit of coatomer complex By similarity.

Catalytic activityi

Broad endopeptidase specificity.

Cofactori

Binds 2 calcium ions Reviewed prediction.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei105 – 1051 By similarity
Active sitei262 – 2621 By similarity
Active sitei286 – 2861 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi588 – 599121 Reviewed prediction
Add
BLAST
Calcium bindingi618 – 629122 Reviewed prediction
Add
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. calcium-dependent cysteine-type endopeptidase activity Source: RefGenome

GO - Biological processi

  1. proteolysis Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiC02.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-8 (EC:3.4.22.53)
Alternative name(s):
Calpain large subunit 4
New calpain 2
Short name:
nCL-2
Stomach-specific M-type calpain
Gene namesi
Name:Capn8
Synonyms:Cls4, Ncl2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi620085. Capn8.

Subcellular locationi

Cytoplasm. Golgi apparatus By similarity 1 Publication

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB-SubCell
  2. cytoplasm Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 703703Calpain-8
PRO_0000349282Add
BLAST

Post-translational modificationi

Undergoes autolytic cleavage between Ala-5 and Ala-6 which gives rise to fragments extending from Ala-6 to the C-terminus, Ala-6 to the EF-hand 2 domain and from Ala-6 to the beginning of domain III By similarity.

Keywords - PTMi

Autocatalytic cleavage

Proteomic databases

PaxDbiQ78EJ9.
PRIDEiQ78EJ9.

Expressioni

Tissue specificityi

Predominantly expressed in the stomach. Localizes strictly to the surface mucus cells in the gastric epithelium and the mucus-secreting goblet cells in the duodenum. Detected in the pituitary after estrogen stimulation.2 Publications

Gene expression databases

GenevestigatoriQ78EJ9.

Interactioni

Subunit structurei

Monomer and homooligomer. Interacts with COPS1/GPS1, COPB1, EYA2, NME2, NME4 and TOMM70A By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000062898.

Structurei

3D structure databases

ProteinModelPortaliQ78EJ9.
SMRiQ78EJ9. Positions 2-699.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 344300Calpain catalytic
Add
BLAST
Domaini532 – 56635EF-hand 1
Add
BLAST
Domaini575 – 60834EF-hand 2
Add
BLAST
Domaini605 – 64036EF-hand 3
Add
BLAST
Domaini670 – 70334EF-hand 4
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni355 – 512158Domain III
Add
BLAST
Regioni513 – 53119Linker By similarity
Add
BLAST
Regioni532 – 703172Domain IV By similarity
Add
BLAST

Domaini

The domain III mediates oligomerization By similarity.

Sequence similaritiesi

Belongs to the peptidase C2 family.
Contains 4 EF-hand domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG327523.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ64698.
KOiK08577.
OrthoDBiEOG7RV9FM.
PhylomeDBiQ78EJ9.
TreeFamiTF314748.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029543. CAPN8.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF52. PTHR10183:SF52. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF13405. EF-hand_6. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q78EJ9-1) [UniParc]FASTAAdd to Basket

Also known as: nCL-2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAALAAGVSK QRAVAEGLGS NQNAVKYLGQ DFETLRKQCL NSGVLFKDPE    50
FPACPSALGY KDLGPGSPDT QGIVWKRPTE LCPNPQFIVG GATRTDIRQG 100
GLGDCWLLAA IASLTLNEKL LYRVLPRDQS FQKDYAGIFH FQFWQYGEWV 150
EVVIDDRLPT KNGQLLFLHS EEGNEFWSAL LEKAYAKLNG SYEALVGGST 200
IEGFEDFTGG ISEFYDLKKP PENLYYIIQK ALRKGSLLGC SIDVSTAAEA 250
EATTRQKLVK GHAYSVTGVE EVNFHGRPEK LIRLRNPWGE VEWSGAWSDN 300
APEWNYIDPR RKEELDKKAE DGEFWMSFSD FLKQYSRLEI CNLSPDSLSS 350
EEIHKWNLVL FNGRWTRGST AGGCLNYPGT YWTNPQFKIH LDEVDEDQEE 400
GTSEPCCTVL LGLMQKNRRR QKRIGQGMLS IGYAVYQIPK ELESHTDAHL 450
GRDFFLGRQP STCSSTYMNL REVSSRVRLP PGQYLVVPST FEPFKDGDFC 500
LRVFSEKKAK ALEIGDTVSG HPHEPHPRDM DEEDEHVRSL FEEFVGKDSE 550
ISANQLKRVL NEVLSKRTDM KFDGFNINTC REMISLLDSD GTGSLGPMEF 600
KTLWLKIRTY LEIFQEMDHN HVGTIEAHEM RTALKKAGFT LNNQVQQTIA 650
MRYACSKLGV DFNGFVACMI RLETLFKLFR LLDKDQNGIV QLSLAEWLCC 700
VLV 703
Length:703
Mass (Da):79,555
Last modified:July 5, 2004 - v1
Checksum:iC0688B055FC0D6EC
GO
Isoform 2 (identifier: Q78EJ9-2) [UniParc]FASTAAdd to Basket

Also known as: Calpain 8b, nCL-2'

The sequence of this isoform differs from the canonical sequence as follows:
     380-381: TY → SS
     382-703: Missing.

Show »
Length:381
Mass (Da):42,592
Checksum:iE738BAFF3EC827E1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei380 – 3812TY → SS in isoform 2.
VSP_035308
Alternative sequencei382 – 703322Missing in isoform 2.
VSP_035309Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti103 – 1031G → V in BAA03369. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D14478 Transcribed RNA. Translation: BAA03369.1.
D14479 mRNA. Translation: BAA03370.1.
D14480 mRNA. Translation: BAA03371.1.
AF514419 Genomic DNA. Translation: AAM94284.1.
PIRiA48764.
RefSeqiNP_579843.2. NM_133309.2. [Q78EJ9-1]
UniGeneiRn.80837.

Genome annotation databases

GeneIDi170808.
KEGGirno:170808.
UCSCiRGD:620085. rat. [Q78EJ9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D14478 Transcribed RNA. Translation: BAA03369.1 .
D14479 mRNA. Translation: BAA03370.1 .
D14480 mRNA. Translation: BAA03371.1 .
AF514419 Genomic DNA. Translation: AAM94284.1 .
PIRi A48764.
RefSeqi NP_579843.2. NM_133309.2. [Q78EJ9-1 ]
UniGenei Rn.80837.

3D structure databases

ProteinModelPortali Q78EJ9.
SMRi Q78EJ9. Positions 2-699.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000062898.

Protein family/group databases

MEROPSi C02.007.

Proteomic databases

PaxDbi Q78EJ9.
PRIDEi Q78EJ9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 170808.
KEGGi rno:170808.
UCSCi RGD:620085. rat. [Q78EJ9-1 ]

Organism-specific databases

CTDi 388743.
RGDi 620085. Capn8.

Phylogenomic databases

eggNOGi NOG327523.
HOGENOMi HOG000232035.
HOVERGENi HBG012645.
InParanoidi Q64698.
KOi K08577.
OrthoDBi EOG7RV9FM.
PhylomeDBi Q78EJ9.
TreeFami TF314748.

Miscellaneous databases

NextBioi 621219.
PROi Q78EJ9.

Gene expression databases

Genevestigatori Q78EJ9.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
InterProi IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029543. CAPN8.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view ]
PANTHERi PTHR10183:SF52. PTHR10183:SF52. 1 hit.
Pfami PF01067. Calpain_III. 1 hit.
PF13405. EF-hand_6. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view ]
PRINTSi PR00704. CALPAIN.
SMARTi SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view ]
SUPFAMi SSF49758. SSF49758. 1 hit.
PROSITEi PS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A novel tissue-specific calpain species expressed predominantly in the stomach comprises two alternative splicing products with and without Ca(2+)-binding domain."
    Sorimachi H., Ishiura S., Suzuki K.
    J. Biol. Chem. 268:19476-19482(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Gastric mucosa.
  2. "Estrogen regulates a tissue-specific calpain in the anterior pituitary."
    Duan W.R., Ito M., Lee E.J., Chien P.-Y., Jameson J.L.
    Biochem. Biophys. Res. Commun. 295:261-266(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-17, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiCAN8_RAT
AccessioniPrimary (citable) accession number: Q78EJ9
Secondary accession number(s): Q64698, Q78EJ8, Q8K407
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: July 5, 2004
Last modified: September 3, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi