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Q78EJ9

- CAN8_RAT

UniProt

Q78EJ9 - CAN8_RAT

Protein

Calpain-8

Gene

Capn8

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Calcium-regulated non-lysosomal thiol-protease. Involved in membrane trafficking in the gastric surface mucus cells (pit cells) and may involve the membrane trafficking of mucus cells via interactions with coat protein. Proteolytically cleaves the beta-subunit of coatomer complex By similarity.By similarity

    Catalytic activityi

    Broad endopeptidase specificity.

    Cofactori

    Binds 2 calcium ions.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei105 – 1051By similarity
    Active sitei262 – 2621By similarity
    Active sitei286 – 2861By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi588 – 599121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi618 – 629122PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium-dependent cysteine-type endopeptidase activity Source: RefGenome
    2. calcium ion binding Source: InterPro

    GO - Biological processi

    1. proteolysis Source: RefGenome

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Ligandi

    Calcium, Metal-binding

    Protein family/group databases

    MEROPSiC02.007.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calpain-8 (EC:3.4.22.53)
    Alternative name(s):
    Calpain large subunit 4
    New calpain 2
    Short name:
    nCL-2
    Stomach-specific M-type calpain
    Gene namesi
    Name:Capn8
    Synonyms:Cls4, Ncl2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620085. Capn8.

    Subcellular locationi

    Cytoplasm 1 Publication. Golgi apparatus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: RefGenome
    2. Golgi apparatus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 703703Calpain-8PRO_0000349282Add
    BLAST

    Post-translational modificationi

    Undergoes autolytic cleavage between Ala-5 and Ala-6 which gives rise to fragments extending from Ala-6 to the C-terminus, Ala-6 to the EF-hand 2 domain and from Ala-6 to the beginning of domain III.By similarity

    Keywords - PTMi

    Autocatalytic cleavage

    Proteomic databases

    PaxDbiQ78EJ9.
    PRIDEiQ78EJ9.

    Expressioni

    Tissue specificityi

    Predominantly expressed in the stomach. Localizes strictly to the surface mucus cells in the gastric epithelium and the mucus-secreting goblet cells in the duodenum. Detected in the pituitary after estrogen stimulation.2 Publications

    Gene expression databases

    GenevestigatoriQ78EJ9.

    Interactioni

    Subunit structurei

    Monomer and homooligomer. Interacts with COPS1/GPS1, COPB1, EYA2, NME2, NME4 and TOMM70A By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000062898.

    Structurei

    3D structure databases

    ProteinModelPortaliQ78EJ9.
    SMRiQ78EJ9. Positions 2-699.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini45 – 344300Calpain catalyticPROSITE-ProRule annotationAdd
    BLAST
    Domaini532 – 56635EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini575 – 60834EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini605 – 64036EF-hand 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini670 – 70334EF-hand 4PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni355 – 512158Domain IIIAdd
    BLAST
    Regioni513 – 53119LinkerBy similarityAdd
    BLAST
    Regioni532 – 703172Domain IVBy similarityAdd
    BLAST

    Domaini

    The domain III mediates oligomerization.By similarity

    Sequence similaritiesi

    Belongs to the peptidase C2 family.Curated
    Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
    Contains 4 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG327523.
    HOGENOMiHOG000232035.
    HOVERGENiHBG012645.
    InParanoidiQ64698.
    KOiK08577.
    OrthoDBiEOG7RV9FM.
    PhylomeDBiQ78EJ9.
    TreeFamiTF314748.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR029543. CAPN8.
    IPR011992. EF-hand-dom_pair.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view]
    PANTHERiPTHR10183:SF52. PTHR10183:SF52. 1 hit.
    PfamiPF01067. Calpain_III. 1 hit.
    PF13405. EF-hand_6. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view]
    PRINTSiPR00704. CALPAIN.
    SMARTiSM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view]
    SUPFAMiSSF49758. SSF49758. 1 hit.
    PROSITEiPS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 4 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q78EJ9-1) [UniParc]FASTAAdd to Basket

    Also known as: nCL-2

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAALAAGVSK QRAVAEGLGS NQNAVKYLGQ DFETLRKQCL NSGVLFKDPE    50
    FPACPSALGY KDLGPGSPDT QGIVWKRPTE LCPNPQFIVG GATRTDIRQG 100
    GLGDCWLLAA IASLTLNEKL LYRVLPRDQS FQKDYAGIFH FQFWQYGEWV 150
    EVVIDDRLPT KNGQLLFLHS EEGNEFWSAL LEKAYAKLNG SYEALVGGST 200
    IEGFEDFTGG ISEFYDLKKP PENLYYIIQK ALRKGSLLGC SIDVSTAAEA 250
    EATTRQKLVK GHAYSVTGVE EVNFHGRPEK LIRLRNPWGE VEWSGAWSDN 300
    APEWNYIDPR RKEELDKKAE DGEFWMSFSD FLKQYSRLEI CNLSPDSLSS 350
    EEIHKWNLVL FNGRWTRGST AGGCLNYPGT YWTNPQFKIH LDEVDEDQEE 400
    GTSEPCCTVL LGLMQKNRRR QKRIGQGMLS IGYAVYQIPK ELESHTDAHL 450
    GRDFFLGRQP STCSSTYMNL REVSSRVRLP PGQYLVVPST FEPFKDGDFC 500
    LRVFSEKKAK ALEIGDTVSG HPHEPHPRDM DEEDEHVRSL FEEFVGKDSE 550
    ISANQLKRVL NEVLSKRTDM KFDGFNINTC REMISLLDSD GTGSLGPMEF 600
    KTLWLKIRTY LEIFQEMDHN HVGTIEAHEM RTALKKAGFT LNNQVQQTIA 650
    MRYACSKLGV DFNGFVACMI RLETLFKLFR LLDKDQNGIV QLSLAEWLCC 700
    VLV 703
    Length:703
    Mass (Da):79,555
    Last modified:July 5, 2004 - v1
    Checksum:iC0688B055FC0D6EC
    GO
    Isoform 2 (identifier: Q78EJ9-2) [UniParc]FASTAAdd to Basket

    Also known as: Calpain 8b, nCL-2'

    The sequence of this isoform differs from the canonical sequence as follows:
         380-381: TY → SS
         382-703: Missing.

    Show »
    Length:381
    Mass (Da):42,592
    Checksum:iE738BAFF3EC827E1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti103 – 1031G → V in BAA03369. (PubMed:7690035)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei380 – 3812TY → SS in isoform 2. 1 PublicationVSP_035308
    Alternative sequencei382 – 703322Missing in isoform 2. 1 PublicationVSP_035309Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14478 Transcribed RNA. Translation: BAA03369.1.
    D14479 mRNA. Translation: BAA03370.1.
    D14480 mRNA. Translation: BAA03371.1.
    AF514419 Genomic DNA. Translation: AAM94284.1.
    PIRiA48764.
    RefSeqiNP_579843.2. NM_133309.2. [Q78EJ9-1]
    UniGeneiRn.80837.

    Genome annotation databases

    GeneIDi170808.
    KEGGirno:170808.
    UCSCiRGD:620085. rat. [Q78EJ9-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14478 Transcribed RNA. Translation: BAA03369.1 .
    D14479 mRNA. Translation: BAA03370.1 .
    D14480 mRNA. Translation: BAA03371.1 .
    AF514419 Genomic DNA. Translation: AAM94284.1 .
    PIRi A48764.
    RefSeqi NP_579843.2. NM_133309.2. [Q78EJ9-1 ]
    UniGenei Rn.80837.

    3D structure databases

    ProteinModelPortali Q78EJ9.
    SMRi Q78EJ9. Positions 2-699.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000062898.

    Protein family/group databases

    MEROPSi C02.007.

    Proteomic databases

    PaxDbi Q78EJ9.
    PRIDEi Q78EJ9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 170808.
    KEGGi rno:170808.
    UCSCi RGD:620085. rat. [Q78EJ9-1 ]

    Organism-specific databases

    CTDi 388743.
    RGDi 620085. Capn8.

    Phylogenomic databases

    eggNOGi NOG327523.
    HOGENOMi HOG000232035.
    HOVERGENi HBG012645.
    InParanoidi Q64698.
    KOi K08577.
    OrthoDBi EOG7RV9FM.
    PhylomeDBi Q78EJ9.
    TreeFami TF314748.

    Miscellaneous databases

    NextBioi 621219.
    PROi Q78EJ9.

    Gene expression databases

    Genevestigatori Q78EJ9.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR022684. Calpain_cysteine_protease.
    IPR022682. Calpain_domain_III.
    IPR022683. Calpain_III.
    IPR029543. CAPN8.
    IPR011992. EF-hand-dom_pair.
    IPR002048. EF_hand_dom.
    IPR000169. Pept_cys_AS.
    IPR001300. Peptidase_C2_calpain_cat.
    [Graphical view ]
    PANTHERi PTHR10183:SF52. PTHR10183:SF52. 1 hit.
    Pfami PF01067. Calpain_III. 1 hit.
    PF13405. EF-hand_6. 1 hit.
    PF00648. Peptidase_C2. 1 hit.
    [Graphical view ]
    PRINTSi PR00704. CALPAIN.
    SMARTi SM00720. calpain_III. 1 hit.
    SM00230. CysPc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view ]
    SUPFAMi SSF49758. SSF49758. 1 hit.
    PROSITEi PS50203. CALPAIN_CAT. 1 hit.
    PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 4 hits.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel tissue-specific calpain species expressed predominantly in the stomach comprises two alternative splicing products with and without Ca(2+)-binding domain."
      Sorimachi H., Ishiura S., Suzuki K.
      J. Biol. Chem. 268:19476-19482(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Gastric mucosa.
    2. "Estrogen regulates a tissue-specific calpain in the anterior pituitary."
      Duan W.R., Ito M., Lee E.J., Chien P.-Y., Jameson J.L.
      Biochem. Biophys. Res. Commun. 295:261-266(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-17, TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.

    Entry informationi

    Entry nameiCAN8_RAT
    AccessioniPrimary (citable) accession number: Q78EJ9
    Secondary accession number(s): Q64698, Q78EJ8, Q8K407
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3