Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q78EG7

- TP4A1_RAT

UniProt

Q78EG7 - TP4A1_RAT

Protein

Protein tyrosine phosphatase type IVA 1

Gene

Ptp4a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. May play a role in the development and maintenance of differentiating epithelial tissues By similarity.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

    Enzyme regulationi

    Inhibited by sodium orthovanadate and pentamidine.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei72 – 721Proton donorBy similarity
    Active sitei104 – 1041Phosphocysteine intermediateBy similarity
    Binding sitei110 – 1101SubstrateBy similarity

    GO - Molecular functioni

    1. protein tyrosine phosphatase activity Source: RGD

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. multicellular organismal development Source: UniProtKB-KW
    3. peptidyl-tyrosine dephosphorylation Source: GOC

    Keywords - Molecular functioni

    Developmental protein, Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein tyrosine phosphatase type IVA 1 (EC:3.1.3.48)
    Alternative name(s):
    Protein-tyrosine phosphatase 4a1
    Protein-tyrosine phosphatase of regenerating liver 1
    Short name:
    PRL-1
    Gene namesi
    Name:Ptp4a1
    Synonyms:Prl1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 9

    Organism-specific databases

    RGDi61970. Ptp4a1.

    Subcellular locationi

    Cell membrane By similarity. Early endosome By similarity. Endoplasmic reticulum By similarity. Cytoplasm By similarity. Cytoplasmcytoskeletonspindle By similarity
    Note: And mitotic spindle.By similarity

    GO - Cellular componenti

    1. early endosome Source: UniProtKB-SubCell
    2. endoplasmic reticulum Source: UniProtKB-SubCell
    3. nucleus Source: RGD
    4. plasma membrane Source: UniProtKB-SubCell
    5. spindle Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Endosome, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi104 – 1041C → S: Abolishes enzymatic activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 170170Protein tyrosine phosphatase type IVA 1PRO_0000094784Add
    BLAST
    Propeptidei171 – 1733Removed in mature formBy similarityPRO_0000396730

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi49 ↔ 104By similarity
    Modified residuei170 – 1701Cysteine methyl esterBy similarity
    Lipidationi170 – 1701S-farnesyl cysteineBy similarity

    Post-translational modificationi

    Farnesylated. Farnesylation is required for membrane targeting By similarity.By similarity

    Keywords - PTMi

    Disulfide bond, Lipoprotein, Methylation, Prenylation

    Proteomic databases

    PRIDEiQ78EG7.

    Expressioni

    Tissue specificityi

    Brain (neurons and oligodendrocytes), skeletal muscle, regenerating liver, tumor cell lines. Expressed in enterocytes of the small intestine villi and colonic surface, zymogen cells of the stomach, proximal tubule cells of the kidney, bronchiolar epithelium, but not in esophagus and heart (at protein level).3 Publications

    Developmental stagei

    Expressed in fetal brain. Up-regulated during oligodendroglial differentiation. Expressed in the developing intestine, esophagus, liver, kidney and lung (at protein level).3 Publications

    Inductioni

    By hepatectomy, mitogens, and ischemia-reperfusion.1 Publication

    Gene expression databases

    GenevestigatoriQ78EG7.

    Interactioni

    Subunit structurei

    Homotrimer. Interacts with ATF5 and tubulin By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000016237.

    Structurei

    Secondary structure

    1
    173
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi10 – 134
    Beta strandi18 – 214
    Turni27 – 293
    Helixi30 – 3910
    Beta strandi42 – 476
    Helixi56 – 605
    Beta strandi64 – 674
    Beta strandi72 – 743
    Helixi78 – 9417
    Beta strandi99 – 1035
    Beta strandi105 – 1084
    Turni109 – 1113
    Helixi112 – 12110
    Helixi126 – 1349
    Beta strandi137 – 1393
    Helixi143 – 1519

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1X24X-ray3.20A/B1-160[»]
    1ZCKX-ray1.90A/B/C7-160[»]
    1ZCLX-ray2.90A/B1-160[»]
    3RZ2X-ray2.80A/B1-169[»]
    ProteinModelPortaliQ78EG7.
    SMRiQ78EG7. Positions 9-160.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ78EG7.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini82 – 14867Tyrosine-protein phosphataseAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni97 – 13236Interaction with ATF5By similarityAdd
    BLAST
    Regioni105 – 1106Phosphate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG265664.
    GeneTreeiENSGT00390000009788.
    HOGENOMiHOG000231265.
    HOVERGENiHBG071295.
    InParanoidiQ78EG7.
    KOiK18041.
    OMAiDKSIAVH.
    OrthoDBiEOG7C8GJD.
    PhylomeDBiQ78EG7.
    TreeFamiTF313384.

    Family and domain databases

    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    [Graphical view]
    PfamiPF00102. Y_phosphatase. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS50056. TYR_PHOSPHATASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q78EG7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARMNRPAPV EVTYKNMRFL ITHNPTNATL NKFIEELKKY GVTTIVRVCE    50
    ATYDTTLVEK EGIHVLDWPF DDGAPPSNQI VDDWLSLVKI KFREEPGCCI 100
    AVHCVAGLGR APVLVALALI EGGMKYEDAV QFIRQKRRGA FNSKQLLYLE 150
    KYRPKMRLRF KDSNGHRNNC CIQ 173
    Length:173
    Mass (Da):19,815
    Last modified:July 5, 2004 - v1
    Checksum:i702008013D3F3835
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti170 – 1701C → W in AAL38661. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L27843 mRNA. Translation: AAA41935.1.
    AY062269 mRNA. Translation: AAL38661.1.
    BC081772 mRNA. Translation: AAH81772.1.
    BC097307 mRNA. Translation: AAH97307.1.
    RefSeqiNP_113767.1. NM_031579.2.
    XP_002727244.1. XM_002727198.3.
    XP_006244732.1. XM_006244670.1.
    XP_006244733.1. XM_006244671.1.
    XP_006244747.1. XM_006244685.1.
    UniGeneiRn.9459.

    Genome annotation databases

    EnsembliENSRNOT00000016237; ENSRNOP00000016237; ENSRNOG00000011771.
    ENSRNOT00000073218; ENSRNOP00000067713; ENSRNOG00000046370.
    GeneIDi100365697.
    102547074.
    29463.
    KEGGirno:100365697.
    rno:102547074.
    rno:29463.
    UCSCiRGD:61970. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L27843 mRNA. Translation: AAA41935.1 .
    AY062269 mRNA. Translation: AAL38661.1 .
    BC081772 mRNA. Translation: AAH81772.1 .
    BC097307 mRNA. Translation: AAH97307.1 .
    RefSeqi NP_113767.1. NM_031579.2.
    XP_002727244.1. XM_002727198.3.
    XP_006244732.1. XM_006244670.1.
    XP_006244733.1. XM_006244671.1.
    XP_006244747.1. XM_006244685.1.
    UniGenei Rn.9459.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1X24 X-ray 3.20 A/B 1-160 [» ]
    1ZCK X-ray 1.90 A/B/C 7-160 [» ]
    1ZCL X-ray 2.90 A/B 1-160 [» ]
    3RZ2 X-ray 2.80 A/B 1-169 [» ]
    ProteinModelPortali Q78EG7.
    SMRi Q78EG7. Positions 9-160.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000016237.

    Chemistry

    BindingDBi Q78EG7.

    Proteomic databases

    PRIDEi Q78EG7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000016237 ; ENSRNOP00000016237 ; ENSRNOG00000011771 .
    ENSRNOT00000073218 ; ENSRNOP00000067713 ; ENSRNOG00000046370 .
    GeneIDi 100365697.
    102547074.
    29463.
    KEGGi rno:100365697.
    rno:102547074.
    rno:29463.
    UCSCi RGD:61970. rat.

    Organism-specific databases

    CTDi 7803.
    RGDi 61970. Ptp4a1.

    Phylogenomic databases

    eggNOGi NOG265664.
    GeneTreei ENSGT00390000009788.
    HOGENOMi HOG000231265.
    HOVERGENi HBG071295.
    InParanoidi Q78EG7.
    KOi K18041.
    OMAi DKSIAVH.
    OrthoDBi EOG7C8GJD.
    PhylomeDBi Q78EG7.
    TreeFami TF313384.

    Miscellaneous databases

    EvolutionaryTracei Q78EG7.
    NextBioi 609264.
    PROi Q78EG7.

    Gene expression databases

    Genevestigatori Q78EG7.

    Family and domain databases

    Gene3Di 3.90.190.10. 1 hit.
    InterProi IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    [Graphical view ]
    Pfami PF00102. Y_phosphatase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    PROSITEi PS50056. TYR_PHOSPHATASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "PRL-1, a unique nuclear protein tyrosine phosphatase, affects cell growth."
      Diamond R.H., Cressman D.E., Laz T.M., Abrams C.S., Taub R.
      Mol. Cell. Biol. 14:3752-3762(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, ENZYME REGULATION, MUTAGENESIS OF CYS-104.
      Tissue: Regenerating liver.
    2. Heneberg P., Draber P.
      Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Basophil.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung and Placenta.
    4. "PRL-1, a protein tyrosine phosphatase, is expressed in neurons and oligodendrocytes in the brain and induced in the cerebral cortex following transient forebrain ischemia."
      Takano S., Fukuyama H., Fukumoto M., Kimura J., Xue J.H., Ohashi H., Fujita J.
      Brain Res. Mol. Brain Res. 40:105-115(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    5. "PRL-1 PTPase expression is developmentally regulated with tissue-specific patterns in epithelial tissues."
      Kong W., Swain G.P., Li S., Diamond R.H.
      Am. J. Physiol. 279:G613-G621(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    6. "Analysis of oligodendroglial differentiation using cDNA arrays."
      Scarlato M., Beesley J., Pleasure D.
      J. Neurosci. Res. 59:430-435(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    7. "Structure and biochemical properties of PRL-1, a phosphatase implicated in cell growth, differentiation, and tumor invasion."
      Sun J.-P., Wang W.-Q., Yang H., Liu S., Liang F., Fedorov A.A., Almo S.C., Zhang Z.-Y.
      Biochemistry 44:12009-12021(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 1-160.

    Entry informationi

    Entry nameiTP4A1_RAT
    AccessioniPrimary (citable) accession number: Q78EG7
    Secondary accession number(s): Q4QRA5, Q8VH48
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3