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Protein

Protein tyrosine phosphatase type IVA 1

Gene

Ptp4a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. May play a role in the development and maintenance of differentiating epithelial tissues (By similarity).By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Enzyme regulationi

Inhibited by sodium orthovanadate and pentamidine.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei72Proton donorBy similarity1
Active sitei104Phosphocysteine intermediateBy similarity1
Binding sitei110SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Protein tyrosine phosphatase type IVA 1 (EC:3.1.3.48)
Alternative name(s):
Protein-tyrosine phosphatase 4a1
Protein-tyrosine phosphatase of regenerating liver 1
Short name:
PRL-1
Gene namesi
Name:Ptp4a1
Synonyms:Prl1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi61970. Ptp4a1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • early endosome Source: UniProtKB-SubCell
  • endoplasmic reticulum Source: UniProtKB-SubCell
  • nucleus Source: RGD
  • plasma membrane Source: UniProtKB-SubCell
  • spindle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi104C → S: Abolishes enzymatic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000947841 – 170Protein tyrosine phosphatase type IVA 1Add BLAST170
PropeptideiPRO_0000396730171 – 173Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi49 ↔ 104By similarity
Modified residuei170Cysteine methyl esterBy similarity1
Lipidationi170S-farnesyl cysteineBy similarity1

Post-translational modificationi

Farnesylated. Farnesylation is required for membrane targeting (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiQ78EG7.
PRIDEiQ78EG7.

Expressioni

Tissue specificityi

Brain (neurons and oligodendrocytes), skeletal muscle, regenerating liver, tumor cell lines. Expressed in enterocytes of the small intestine villi and colonic surface, zymogen cells of the stomach, proximal tubule cells of the kidney, bronchiolar epithelium, but not in esophagus and heart (at protein level).3 Publications

Developmental stagei

Expressed in fetal brain. Up-regulated during oligodendroglial differentiation. Expressed in the developing intestine, esophagus, liver, kidney and lung (at protein level).3 Publications

Inductioni

By hepatectomy, mitogens, and ischemia-reperfusion.1 Publication

Gene expression databases

BgeeiENSRNOG00000011771.
GenevisibleiQ78EG7. RN.

Interactioni

Subunit structurei

Homotrimer. Interacts with ATF5 and tubulin (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000067713.

Structurei

Secondary structure

1173
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 13Combined sources4
Beta strandi18 – 21Combined sources4
Turni27 – 29Combined sources3
Helixi30 – 39Combined sources10
Beta strandi42 – 47Combined sources6
Helixi56 – 60Combined sources5
Beta strandi64 – 67Combined sources4
Beta strandi72 – 74Combined sources3
Helixi78 – 94Combined sources17
Beta strandi99 – 103Combined sources5
Beta strandi105 – 108Combined sources4
Turni109 – 111Combined sources3
Helixi112 – 121Combined sources10
Helixi126 – 134Combined sources9
Beta strandi137 – 139Combined sources3
Helixi143 – 151Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X24X-ray3.20A/B1-160[»]
1ZCKX-ray1.90A/B/C7-160[»]
1ZCLX-ray2.90A/B1-160[»]
3RZ2X-ray2.80A/B1-169[»]
ProteinModelPortaliQ78EG7.
SMRiQ78EG7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ78EG7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini82 – 148Tyrosine-protein phosphataseAdd BLAST67

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni97 – 132Interaction with ATF5By similarityAdd BLAST36
Regioni105 – 110Phosphate bindingBy similarity6

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2836. Eukaryota.
ENOG4111I7J. LUCA.
GeneTreeiENSGT00390000009788.
HOGENOMiHOG000231265.
HOVERGENiHBG071295.
InParanoidiQ78EG7.
KOiK18041.
OMAiETIAVHC.
OrthoDBiEOG091G0MYM.
PhylomeDBiQ78EG7.
TreeFamiTF313384.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS50056. TYR_PHOSPHATASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q78EG7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARMNRPAPV EVTYKNMRFL ITHNPTNATL NKFIEELKKY GVTTIVRVCE
60 70 80 90 100
ATYDTTLVEK EGIHVLDWPF DDGAPPSNQI VDDWLSLVKI KFREEPGCCI
110 120 130 140 150
AVHCVAGLGR APVLVALALI EGGMKYEDAV QFIRQKRRGA FNSKQLLYLE
160 170
KYRPKMRLRF KDSNGHRNNC CIQ
Length:173
Mass (Da):19,815
Last modified:July 5, 2004 - v1
Checksum:i702008013D3F3835
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti170C → W in AAL38661 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27843 mRNA. Translation: AAA41935.1.
AY062269 mRNA. Translation: AAL38661.1.
BC081772 mRNA. Translation: AAH81772.1.
BC097307 mRNA. Translation: AAH97307.1.
RefSeqiNP_113767.1. NM_031579.3.
XP_002727244.1. XM_002727198.5.
XP_006244733.1. XM_006244671.3.
XP_008765001.1. XM_008766779.2.
UniGeneiRn.9459.

Genome annotation databases

EnsembliENSRNOT00000016237; ENSRNOP00000016237; ENSRNOG00000011771.
ENSRNOT00000079166; ENSRNOP00000074101; ENSRNOG00000057009.
GeneIDi100365697.
103693189.
29463.
KEGGirno:100365697.
rno:103693189.
rno:29463.
UCSCiRGD:61970. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27843 mRNA. Translation: AAA41935.1.
AY062269 mRNA. Translation: AAL38661.1.
BC081772 mRNA. Translation: AAH81772.1.
BC097307 mRNA. Translation: AAH97307.1.
RefSeqiNP_113767.1. NM_031579.3.
XP_002727244.1. XM_002727198.5.
XP_006244733.1. XM_006244671.3.
XP_008765001.1. XM_008766779.2.
UniGeneiRn.9459.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X24X-ray3.20A/B1-160[»]
1ZCKX-ray1.90A/B/C7-160[»]
1ZCLX-ray2.90A/B1-160[»]
3RZ2X-ray2.80A/B1-169[»]
ProteinModelPortaliQ78EG7.
SMRiQ78EG7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000067713.

Proteomic databases

PaxDbiQ78EG7.
PRIDEiQ78EG7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016237; ENSRNOP00000016237; ENSRNOG00000011771.
ENSRNOT00000079166; ENSRNOP00000074101; ENSRNOG00000057009.
GeneIDi100365697.
103693189.
29463.
KEGGirno:100365697.
rno:103693189.
rno:29463.
UCSCiRGD:61970. rat.

Organism-specific databases

CTDi7803.
RGDi61970. Ptp4a1.

Phylogenomic databases

eggNOGiKOG2836. Eukaryota.
ENOG4111I7J. LUCA.
GeneTreeiENSGT00390000009788.
HOGENOMiHOG000231265.
HOVERGENiHBG071295.
InParanoidiQ78EG7.
KOiK18041.
OMAiETIAVHC.
OrthoDBiEOG091G0MYM.
PhylomeDBiQ78EG7.
TreeFamiTF313384.

Miscellaneous databases

EvolutionaryTraceiQ78EG7.
PROiQ78EG7.

Gene expression databases

BgeeiENSRNOG00000011771.
GenevisibleiQ78EG7. RN.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS50056. TYR_PHOSPHATASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTP4A1_RAT
AccessioniPrimary (citable) accession number: Q78EG7
Secondary accession number(s): Q4QRA5, Q8VH48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.