Reviewed, UniProtKB/Swiss-Prot Q783Y1 (HN_SENDA)

Names and origin

Protein names

Recommended name:
    Hemagglutinin-neuraminidase
      Short name=HN protein
    EC=3.2.1.18

Gene names

Name:

HN

Organism

Sendai virus (strain Hamamatsu) (SeV) [Complete proteome]

Taxonomic identifier

302271 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Respirovirus

Virus host

Mus musculus (Mouse) [TaxID: 10090]
Rattus norvegicus (Rat) [TaxID: 10116]
Cavia cutleri (Guinea pig) [TaxID: 10144]
Cricetidae sp. (Hamster) [TaxID: 36483]

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion By similarity. Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins By similarity.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subunit structure	Homotetramer; composed of disulfide-linked homodimers. Interacts with F protein trimer By similarity.
Subcellular location	Virion membrane; Single-pass type II membrane protein Potential. Host cell membrane; Single-pass type II membrane protein Potential. Note: Folded in the endoplasmic reticulum By similarity.
Post-translational modification	N-glycosylated; glycans consist of a mixture of high mannose-type oligosaccharides and of complex-type oligosaccharides By similarity.
Sequence similarities	Belongs to the paramyxoviruses hemagglutinin-neuraminidase family.

Ontologies

Keywords
Cellular component	Envelope protein Host cell membrane Host membrane Membrane Virion
Domain	Signal-anchor Transmembrane
Molecular function	Hemagglutinin Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell viral envelope Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	exo-alpha-sialidase activity Inferred from electronic annotation. Source: EC host cell surface receptor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Hemagglutinin-neuraminidase		PRO_0000142636
Regions
	Topological domain	1 – 37	37	Cytoplasmic By similarity
	Transmembrane	38 – 58	21	By similarity
	Topological domain	59 – 575	517	Extracellular By similarity
	Region	10 – 14	5	Incorporation in virion By similarity
	Region	59 – 140	82	Involved in interaction with F protein By similarity
Amino acid modifications
	Glycosylation	77	1	N-linked (GlcNAc...); by host By similarity
	Glycosylation	499	1	N-linked (GlcNAc...); by host By similarity
	Glycosylation	511	1	N-linked (GlcNAc...); by host By similarity
	Disulfide bond	129		Interchain Potential
Natural variations
	Natural variant	461	1	E → G in strain: Isolate E30cl2; egg passage attenuated.
	Natural variant	462	1	D → G in strain: Isolate E30cl2, Isolate E50cl9 and Isolate E30M15cl5; egg passage attenuated.

Sequences

	Sequence		Length	Mass (Da)
	Q783Y1-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: BDA9E1B72431058A	FASTA	575	63,212
	10 20 30 40 50 60 MDGDRSKRDS YWSTSPGGST TKLVSDSERS GKVDTWLLIL AFTQWALSIA TVIICIVIAA 70 80 90 100 110 120 RQGYSMERYS MTVEALNTSN KEVKESLTSL IRQEVITRAV NIQSSVQTGI PVLLNKNSRD 130 140 150 160 170 180 VIQLIEKSCN RQELTQLCDS TIAVHHAEGI APLEPHSFWR CPAGEPYLSS DPEVSLLPGP 190 200 210 220 230 240 SLLSGSTTIS GCVRLPSLSI GEAIYAYSSN LITQGCADIG KSYQVLQLGY ISLNSDMFPD 250 260 270 280 290 300 LNPVVSHTYD INDNRKSCSV VATGTRGYQL CSMPTVDERT DYSSDGIEDL VLDILDLKGR 310 320 330 340 350 360 TKSHRYSNSE IDLDHPFSAL YPSVGSGIAT EGSLIFLGYG GLTTPLQGDT KCRIQGCQQV 370 380 390 400 410 420 SQDTCNEALK ITWLGGKQVV SVLIQVNDYL SERPRIRVTT VPITQNYLGA EGRLLKLGDQ 430 440 450 460 470 480 VYIYTRSSGW HSQLQIGVLD VSHPLTISWT PHEALSRPGN EDCNWYNTCP KECISGVYTD 490 500 510 520 530 540 AYPLSPDAAN VATVTLYANT SRVNPTIMYS NTTNIINMLR IKDVKLEAAY TTTSCITHFG 550 560 570 KGYCFHIIEI NQKSLNTLQP MLFKTSIPKL CKAES

References

[1]	"A field isolate of Sendai virus: its high virulence to mice and genetic divergence from prototype strains." Sakaguchi T., Fujii Y., Kiyotani K., Sasaki M., Yoshida T. Arch. Virol. 135:159-164(1994) [PubMed: 8198441] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Conserved and non-conserved regions in the Sendai virus genome: evolution of a gene possessing overlapping reading frames." Fujii Y., Kiyotani K., Yoshida T., Sakaguchi T. Virus Genes 22:47-52(2001) [PubMed: 11210938] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]	"Involvement of the leader sequence in Sendai virus pathogenesis revealed by recovery of a pathogenic field isolate from cDNA." Fujii Y., Sakaguchi T., Kiyotani K., Huang C., Fukuhara N., Egi Y., Yoshida T. J. Virol. 76:8540-8547(2002) [PubMed: 12163573] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate E15cl2.
[4]	"Identification of mutations associated with attenuation of virulence of a field Sendai virus isolate by egg passage." Fujii Y., Sakaguchi T., Kiyotani K., Huang C., Fukuhara N., Yoshida T. Virus Genes 25:189-193(2002) [PubMed: 12416681] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate E15cl2, Isolate E30cl2 and Isolate E30M15cl5.
[5]	Fujii Y., Kiyotani K., Huang C., Fukuhara N., Egi K., Yoshida T., Sakaguchi T. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate E50cl9.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X57213 Genomic RNA. Translation: CAA40499.1. AB039658 Genomic RNA. Translation: BAB20025.1. AB065186 Genomic RNA. Translation: BAC79131.1. AB065187 Genomic RNA. Translation: BAC07511.1. AB065188 Genomic RNA. Translation: BAC79139.1. AB065189 Genomic RNA. Translation: BAC79147.1.
PIR	S14532.
3D structure databases
SMR	Q783Y1. Positions 144-572.
ModBase	Search...
Family and domain databases
InterPro	IPR000665. Hemagglutn-neuramid_glycoprot. IPR016285. Hemagglutn-neuramid_paramyxo. IPR011040. Neuraminidase. [Graphical view]
Pfam	PF00423. HN. 1 hit. [Graphical view]
PIRSF	PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HN_SENDA

Accession

Primary (citable) accession number: Q783Y1
Secondary accession number(s): Q7T415, Q7TB20, Q82459

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	July 5, 2004
Last modified:	January 19, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)