Reviewed, UniProtKB/Swiss-Prot Q77SJ8 (L_HIRRV)

Names and origin

Protein names

Recommended name:
    Large structural protein
      Short name=Protein L
Alternative name(s):
    Transcriptase
    Replicase
Including the following 3 domains:
    1- Recommended name:
            RNA-directed RNA polymerase
              EC=2.7.7.48
    2- Recommended name:
            mRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.56
    3- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.-

Gene names

Name:

L

Organism

Hirame rhabdovirus (strain Korea/CA 9703/1997) (HIRRV) [Complete proteome]

Taxonomic identifier

453457 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Novirhabdovirus

Virus host

Paralichthys olivaceus (Japanese flounder) [TaxID: 8255]
Plecoglossus altivelis (Ayu) [TaxID: 61084]
Acanthopagrus schlegeli (Black porgy) [TaxID: 72011]
Sebastes inermis [TaxID: 160818]

Protein attributes

Sequence length	1986 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein By similarity.
Subcellular location	Virion Potential. Host cytoplasm By similarity.
Sequence similarities	Belongs to the rhabdoviridae protein L family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1986	1986	Large structural protein		PRO_0000297836
Regions
	Domain	560 – 757	198	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q77SJ8-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 3A6254B6704D0C59	FASTA	1,986	224,461
	10 20 30 40 50 60 MDFFDLDIEI KQERLPAECS LNSPLNISLS SQLTDRMTPQ NENIRRQRER IRTHTKTHSR 70 80 90 100 110 120 IKHLSKLDND STRLHARLTE DLIKLQHLEV DSPVFDNWAL LTSYYAALDY TLPERASFDW 130 140 150 160 170 180 GQAAPYWNLY TQLRTILLQS QKIRKKDRGV REIYSCGPLR LEFVEGTVLY FTDKQSGGEF 190 200 210 220 230 240 TKSGELPSIT PYADFLAWVK IISQRAQAVL MAVILRVTDK GLSPLPESLL SVYQTVDDIL 250 260 270 280 290 300 KRAGQPAIDL LKLWEPLVIT KLGELLGDRF GLEEDFRLTI RGEATRLAKK LAITNGLNRL 310 320 330 340 350 360 MTVLDSQTEA QPLFQFFGLF KHFAYPRVFS RDTIQAIQEV SDRPSSISAA EFLHDQCEIR 370 380 390 400 410 420 KEFYIRYLKA YHRAPGLDLS ALSPSSFLRE SLEHGKIPNE KSPHYSNKEW YFIKFTKSIE 430 440 450 460 470 480 WPISDTLSTF LSDKAITRDR AAWIEEGHSG RDMSEKRLLL KFIKENFSSV AEIVAAADAI 490 500 510 520 530 540 YNNEGDRLIA LKVKEMELKI KGRGFGLMTF MPRLLQVLRE SIAKKTSKLF PEITMTSSDL 550 560 570 580 590 600 DMKKRKFMLS KRSDDRRGFI HINKSLDINK FCTSQRQFNS SAVFSSLDEM MGTFPLFSRV 610 620 630 640 650 660 HEIFEKTWIV DGSASDPPDL SHFTRILEEC RLHGIEAPHV WADGVFSGLK GGIEGLCQYV 670 680 690 700 710 720 WTICLLLRVE RVMQKTKLTH YILAQGDNVI ITIIVPVEIH RDGIISDQES RRLLTLSRNI 730 740 750 760 770 780 DLSLESELEK SGLTLKIEET LTSENISIYG KDLHCPQHLT LALKKAASAA IISSEQYQDI 790 800 810 820 830 840 PTFLSGLGTS LESLSECVNS KTGAHLFGVL MGVAGWKDLA THQTWRGWRY PYHKAPLSGR 850 860 870 880 890 900 VRASDMKIGK GEAVELTIPV MSPRQQGKET LRELLANSLL GSALGMLAFP TPIDLEKRGV 910 920 930 940 950 960 GDYITHRLAI ARKALLSEKL DPHIEKRVRS ACNLPLSSRV DLSKLFDSPF SLNLATEEDA 970 980 990 1000 1010 1020 TAVIKRQAKK TLRLQEIGND KLRAQIGNMD KGIAALDADL AGAETINPRL NHMIRDITDE 1030 1040 1050 1060 1070 1080 KESEMFVTKF ASARTMRTLA MGDSSEVPIV VLLEKKSQQK ELYTIWRARR PHATMWKCST 1090 1100 1110 1120 1130 1140 VLAKGLRDIS WGKTIVGVTS PSPIEAMETT HIDPTDWEDS RSRETLSINY YLSRAGIDEQ 1150 1160 1170 1180 1190 1200 TAKLTRGSLV PYYGTQTKPL IAKAYLELKG NPRTNKALLL LSVRESLVKT GSNLDELIMQ 1210 1220 1230 1240 1250 1260 LCSHALDIDA ASLPALRAQE EATAGEGLRG GIKESMSPVG PDNFYTNITH KVFNRKWVTP 1270 1280 1290 1300 1310 1320 YHVNIADFII QGLIETRKHL ILNEKMDGLL PLSSVKCTAC FRKKEREFFD IPEGPTWKND 1330 1340 1350 1360 1370 1380 STTSDPAYTY FTTWCDLPRV STLPTMDQQS ATRLLGRGLS LNRPTAGEII TKFYSMSMES 1390 1400 1410 1420 1430 1440 QRLLHPVDLL LGYGEGVVFG YIRSQHIHHG ALFQTKRETL TNKLRKFILD TKTQHAKQIG 1450 1460 1470 1480 1490 1500 YLFQDEDSLH ELMAQGLCPY VPRSIPLTIT ELTNACAITT IRATEVILSA GSRVALMPVQ 1510 1520 1530 1540 1550 1560 AIDETDVDNS RLAANTMQTI LGDSRPMNPV YLDCDLTTNM TAWESSIELD VLKSENFHID 1570 1580 1590 1600 1610 1620 GLLMDLTARE LPISDTPWKQ RDWTCSNDPR IIAKGIKTKS LFIHQGVAEA LNMTPDLLVV 1630 1640 1650 1660 1670 1680 IGGGLGGCAV PYLQEWADVP LIFATLFDER ERISEDGDLV VPPEILVRGM APRMIERELL 1690 1700 1710 1720 1730 1740 EAELCDVTND GNRRLLTRLV KKNRGKGTVV LIDEIENRGA PESLLQSSLQ DLVRRLDKVC 1750 1760 1770 1780 1790 1800 TLTSIHTVRE STVEQFAQRT NSIKRDRKTV TLHWNRYNRR DQFEALVIVK GEETRSDYHV 1810 1820 1830 1840 1850 1860 STATAAQAFR KIDEQLEVEG RLSATRWSLP TLPAREKEIL FGYVSSVFLK TNLVLSADDM 1870 1880 1890 1900 1910 1920 DRETLLETIE DTAPGLISWK EKLEHRDHAF RSDIDEKGIT QDKVFNLICL AWVITGLRYG 1930 1940 1950 1960 1970 1980 IWETDAQSII TKTVYITRGP KLCPLGEKPK RVFASFKLQS DKRVEDAKGF LSALLHLEGF FPLGRQ

References

[1]	"Complete nucleotide sequence of the hirame rhabdovirus, a pathogen of marine fish." Kim D.H., Oh H.K., Eou J.I., Seo H.J., Kim S.K., Oh M.J., Nam S.W., Choi T.J. Virus Res. 107:1-9(2005) [PubMed: 15567027] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF104985 Genomic RNA. Translation: AAQ73462.1.
RefSeq	NP_919035.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2559539.
Family and domain databases
InterPro	IPR014023. RNA-dir_pol_cat. IPR001016. RNA_pol_L_viral. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_HIRRV

Accession

Primary (citable) accession number: Q77SJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	July 5, 2004
Last modified:	January 19, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)