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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

RIR1

Organism
Gallid herpesvirus 2 (strain Chicken/Md5/ATCC VR-987) (GaHV-2) (Marek's disease herpesvirus type 1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.UniRule annotation

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.UniRule annotation

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei249SubstrateUniRule annotation1
Sitei265Important for hydrogen atom transferUniRule annotation1
Binding sitei295Substrate; via amide nitrogenUniRule annotation1
Active sitei470Proton acceptorUniRule annotation1
Active sitei472Cysteine radical intermediateUniRule annotation1
Active sitei474Proton acceptorUniRule annotation1
Sitei487Important for hydrogen atom transferUniRule annotation1
Sitei796Important for electron transferUniRule annotation1
Sitei797Important for electron transferUniRule annotation1
Sitei817Interacts with thioredoxin/glutaredoxinUniRule annotation1
Sitei820Interacts with thioredoxin/glutaredoxinUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunitUniRule annotation (EC:1.17.4.1UniRule annotation)
Short name:
R1UniRule annotation
Alternative name(s):
Ribonucleotide reductase large subunitUniRule annotation
Gene namesi
Name:RIR1UniRule annotation
Ordered Locus Names:MDV052
OrganismiGallid herpesvirus 2 (strain Chicken/Md5/ATCC VR-987) (GaHV-2) (Marek's disease herpesvirus type 1)
Taxonomic identifieri10389 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeMardivirus
Virus hostiGallus gallus (Chicken) [TaxID: 9031]
Proteomesi
  • UP000008072 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004064201 – 822Ribonucleoside-diphosphate reductase large subunitAdd BLAST822

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi265 ↔ 487Redox-activeUniRule annotation

Keywords - PTMi

Disulfide bond

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ77MS1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni264 – 265Substrate bindingUniRule annotation2
Regioni470 – 474Substrate bindingUniRule annotation5
Regioni651 – 655Substrate bindingUniRule annotation5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi205 – 209Poly-Ala5

Sequence similaritiesi

Belongs to the ribonucleoside diphosphate reductase large chain family.UniRule annotation

Phylogenomic databases

KOiK19448.

Family and domain databases

HAMAPiMF_04026. HSV_RIR1. 1 hit.
InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q77MS1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDHSDVYEPY TFSGKHHVRM YDFSKLTPMW NKDASTPPRK FADMHIASTV
60 70 80 90 100
AMELYNIERE LLNMGHLSGM ETMMVDVRKS PEMIPSTINM RDHITRLVNK
110 120 130 140 150
MKPICRFDER LYSLCGELVH LRIELREVPL DTWLLSKKLN LKYEVVDNIR
160 170 180 190 200
RYRAHIEMDM LRFYGSIHPW LKRLGLQSAL KYEEYLVELE DGKKESLCQF
210 220 230 240 250
FVRLAAAAAT EASMKKPFMT TLTTGVANWR TTFTTFFLAL ANQLFVPSTP
260 270 280 290 300
CMLFLGREGT STASCYLMDP RTNNTQDTLK AITEDVVPHL LARGGIGISL
310 320 330 340 350
QHLNGKFGLM HVMKVLDSLV MAANVNESRP TGICVYLEPW HADIMSALNM
360 370 380 390 400
RGMVAAEESR RCDNVFLALW SCDLLFKRYL RHVNGEKNVI WTLFDSRASI
410 420 430 440 450
LTKLHSEEFE KEYERLESEG LGVASIPIRD MMFAIIKSAA STGSPFILFK
460 470 480 490 500
DACNRHYITN TQGDAIAGSN LCTEIIQKTD ANTNGVCSLA SINLARCVRN
510 520 530 540 550
IDGNRQFDFD ALRYAVRLAT VFVNAIMEGS DVPTEKSHSG RERNRSMGIG
560 570 580 590 600
VQGFHTAFLS MGLDLCDERA RSLNKLIFEF MLLEAMTVSC EFCERGLPPF
610 620 630 640 650
ADFSNSYYAR GRLHFDGWAN VELAAVEEWN MLRDRIVSAG LYNAQFIALM
660 670 680 690 700
PTAASAQVTE VSEGFSPLFS NMFSKVTSTG ELLRPNIQLM EELRTIYLDN
710 720 730 740 750
ENQRLATIAA LESANWNIQT ALGNKPECHS LLKYKTAFDY DQALLIDLCS
760 770 780 790 800
DRAPFVDQSQ SMTLFITETA DGTLLASRVM NLLLHAYKAG LKTGMYYCKI
810 820
RKATNAGVFC GDGELTCSSC VL
Length:822
Mass (Da):92,821
Last modified:October 13, 2009 - v1
Checksum:iA03EA8256609AC4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF243438 Genomic DNA. Translation: AAG14232.1.
RefSeqiYP_001033968.1. NC_002229.3.

Genome annotation databases

GeneIDi4811513.
KEGGivg:4811513.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF243438 Genomic DNA. Translation: AAG14232.1.
RefSeqiYP_001033968.1. NC_002229.3.

3D structure databases

ProteinModelPortaliQ77MS1.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4811513.
KEGGivg:4811513.

Phylogenomic databases

KOiK19448.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

HAMAPiMF_04026. HSV_RIR1. 1 hit.
InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIR1_GAHVM
AccessioniPrimary (citable) accession number: Q77MS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: October 13, 2009
Last modified: October 5, 2016
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.