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Q77MS1 (RIR1_GAHVM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonucleoside-diphosphate reductase large subunit

EC=1.17.4.1
Alternative name(s):
Ribonucleotide reductase large subunit
Gene names
Name:MDV052
OrganismGallid herpesvirus 2 (strain Chicken/Md5/ATCC VR-987) (GaHV-2) (Marek's disease herpesvirus type 1) [Reference proteome]
Taxonomic identifier10389 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeMardivirus
Virus hostGallus gallus (Chicken) [TaxID: 9031]

Protein attributes

Sequence length822 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Pathway

Genetic information processing; DNA replication.

Subunit structure

Heterodimer of a large and a small chain By similarity.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase large chain family.

Ontologies

Keywords
   Biological processDNA replication
   LigandATP-binding
Nucleotide-binding
   Molecular functionOxidoreductase
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 822822Ribonucleoside-diphosphate reductase large subunit
PRO_0000406420

Regions

Region264 – 2652Substrate binding By similarity
Region470 – 4745Substrate binding By similarity
Region651 – 6555Substrate binding By similarity
Compositional bias205 – 2095Poly-Ala

Sites

Active site4701Proton acceptor By similarity
Active site4721Cysteine radical intermediate By similarity
Active site4741Proton acceptor By similarity
Binding site2491Substrate By similarity
Binding site2951Substrate; via amide nitrogen By similarity
Site2651Important for hydrogen atom transfer By similarity
Site4871Important for hydrogen atom transfer By similarity
Site7961Important for electron transfer By similarity
Site7971Important for electron transfer By similarity
Site8171Interacts with thioredoxin/glutaredoxin By similarity
Site8201Interacts with thioredoxin/glutaredoxin By similarity

Amino acid modifications

Disulfide bond265 ↔ 487Redox-active By similarity

Sequences

Sequence LengthMass (Da)Tools
Q77MS1 [UniParc].

Last modified October 13, 2009. Version 1.
Checksum: A03EA8256609AC4A

FASTA82292,821
        10         20         30         40         50         60 
MDHSDVYEPY TFSGKHHVRM YDFSKLTPMW NKDASTPPRK FADMHIASTV AMELYNIERE 

        70         80         90        100        110        120 
LLNMGHLSGM ETMMVDVRKS PEMIPSTINM RDHITRLVNK MKPICRFDER LYSLCGELVH 

       130        140        150        160        170        180 
LRIELREVPL DTWLLSKKLN LKYEVVDNIR RYRAHIEMDM LRFYGSIHPW LKRLGLQSAL 

       190        200        210        220        230        240 
KYEEYLVELE DGKKESLCQF FVRLAAAAAT EASMKKPFMT TLTTGVANWR TTFTTFFLAL 

       250        260        270        280        290        300 
ANQLFVPSTP CMLFLGREGT STASCYLMDP RTNNTQDTLK AITEDVVPHL LARGGIGISL 

       310        320        330        340        350        360 
QHLNGKFGLM HVMKVLDSLV MAANVNESRP TGICVYLEPW HADIMSALNM RGMVAAEESR 

       370        380        390        400        410        420 
RCDNVFLALW SCDLLFKRYL RHVNGEKNVI WTLFDSRASI LTKLHSEEFE KEYERLESEG 

       430        440        450        460        470        480 
LGVASIPIRD MMFAIIKSAA STGSPFILFK DACNRHYITN TQGDAIAGSN LCTEIIQKTD 

       490        500        510        520        530        540 
ANTNGVCSLA SINLARCVRN IDGNRQFDFD ALRYAVRLAT VFVNAIMEGS DVPTEKSHSG 

       550        560        570        580        590        600 
RERNRSMGIG VQGFHTAFLS MGLDLCDERA RSLNKLIFEF MLLEAMTVSC EFCERGLPPF 

       610        620        630        640        650        660 
ADFSNSYYAR GRLHFDGWAN VELAAVEEWN MLRDRIVSAG LYNAQFIALM PTAASAQVTE 

       670        680        690        700        710        720 
VSEGFSPLFS NMFSKVTSTG ELLRPNIQLM EELRTIYLDN ENQRLATIAA LESANWNIQT 

       730        740        750        760        770        780 
ALGNKPECHS LLKYKTAFDY DQALLIDLCS DRAPFVDQSQ SMTLFITETA DGTLLASRVM 

       790        800        810        820 
NLLLHAYKAG LKTGMYYCKI RKATNAGVFC GDGELTCSSC VL 

« Hide

References

[1]"The genome of a very virulent Marek's disease virus."
Tulman E.R., Afonso C.L., Lu Z., Zsak L., Rock D.L., Kutish G.F.
J. Virol. 74:7980-7988(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF243438 Genomic DNA. Translation: AAG14232.1.
RefSeqYP_001033968.1. NC_002229.3.

3D structure databases

ProteinModelPortalQ77MS1.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ77MS1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4811513.

Enzyme and pathway databases

UniPathwayUPA00326.

Family and domain databases

InterProIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSPR01183. RIBORDTASEM1.
TIGRFAMsTIGR02506. NrdE_NrdA. 1 hit.
PROSITEPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRIR1_GAHVM
AccessionPrimary (citable) accession number: Q77MS1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: October 13, 2009
Last modified: May 14, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways