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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

MDV052

Organism
Gallid herpesvirus 2 (strain Chicken/Md5/ATCC VR-987) (GaHV-2) (Marek's disease herpesvirus type 1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Pathway:iDNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei249 – 2491SubstrateBy similarity
Sitei265 – 2651Important for hydrogen atom transferBy similarity
Binding sitei295 – 2951Substrate; via amide nitrogenBy similarity
Active sitei470 – 4701Proton acceptorBy similarity
Active sitei472 – 4721Cysteine radical intermediateBy similarity
Active sitei474 – 4741Proton acceptorBy similarity
Sitei487 – 4871Important for hydrogen atom transferBy similarity
Sitei796 – 7961Important for electron transferBy similarity
Sitei797 – 7971Important for electron transferBy similarity
Sitei817 – 8171Interacts with thioredoxin/glutaredoxinBy similarity
Sitei820 – 8201Interacts with thioredoxin/glutaredoxinBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
Name:MDV052
OrganismiGallid herpesvirus 2 (strain Chicken/Md5/ATCC VR-987) (GaHV-2) (Marek's disease herpesvirus type 1)
Taxonomic identifieri10389 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeMardivirus
Virus hostiGallus gallus (Chicken) [TaxID: 9031]
ProteomesiUP000008072 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 822822Ribonucleoside-diphosphate reductase large subunitPRO_0000406420Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi265 ↔ 487Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ77MS1.

Interactioni

Subunit structurei

Heterodimer of a large and a small chain.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ77MS1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni264 – 2652Substrate bindingBy similarity
Regioni470 – 4745Substrate bindingBy similarity
Regioni651 – 6555Substrate bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi205 – 2095Poly-Ala

Sequence similaritiesi

Phylogenomic databases

KOiK10807.

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q77MS1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDHSDVYEPY TFSGKHHVRM YDFSKLTPMW NKDASTPPRK FADMHIASTV
60 70 80 90 100
AMELYNIERE LLNMGHLSGM ETMMVDVRKS PEMIPSTINM RDHITRLVNK
110 120 130 140 150
MKPICRFDER LYSLCGELVH LRIELREVPL DTWLLSKKLN LKYEVVDNIR
160 170 180 190 200
RYRAHIEMDM LRFYGSIHPW LKRLGLQSAL KYEEYLVELE DGKKESLCQF
210 220 230 240 250
FVRLAAAAAT EASMKKPFMT TLTTGVANWR TTFTTFFLAL ANQLFVPSTP
260 270 280 290 300
CMLFLGREGT STASCYLMDP RTNNTQDTLK AITEDVVPHL LARGGIGISL
310 320 330 340 350
QHLNGKFGLM HVMKVLDSLV MAANVNESRP TGICVYLEPW HADIMSALNM
360 370 380 390 400
RGMVAAEESR RCDNVFLALW SCDLLFKRYL RHVNGEKNVI WTLFDSRASI
410 420 430 440 450
LTKLHSEEFE KEYERLESEG LGVASIPIRD MMFAIIKSAA STGSPFILFK
460 470 480 490 500
DACNRHYITN TQGDAIAGSN LCTEIIQKTD ANTNGVCSLA SINLARCVRN
510 520 530 540 550
IDGNRQFDFD ALRYAVRLAT VFVNAIMEGS DVPTEKSHSG RERNRSMGIG
560 570 580 590 600
VQGFHTAFLS MGLDLCDERA RSLNKLIFEF MLLEAMTVSC EFCERGLPPF
610 620 630 640 650
ADFSNSYYAR GRLHFDGWAN VELAAVEEWN MLRDRIVSAG LYNAQFIALM
660 670 680 690 700
PTAASAQVTE VSEGFSPLFS NMFSKVTSTG ELLRPNIQLM EELRTIYLDN
710 720 730 740 750
ENQRLATIAA LESANWNIQT ALGNKPECHS LLKYKTAFDY DQALLIDLCS
760 770 780 790 800
DRAPFVDQSQ SMTLFITETA DGTLLASRVM NLLLHAYKAG LKTGMYYCKI
810 820
RKATNAGVFC GDGELTCSSC VL
Length:822
Mass (Da):92,821
Last modified:October 13, 2009 - v1
Checksum:iA03EA8256609AC4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF243438 Genomic DNA. Translation: AAG14232.1.
RefSeqiYP_001033968.1. NC_002229.3.

Genome annotation databases

GeneIDi4811513.
KEGGivg:4811513.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF243438 Genomic DNA. Translation: AAG14232.1.
RefSeqiYP_001033968.1. NC_002229.3.

3D structure databases

ProteinModelPortaliQ77MS1.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ77MS1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4811513.
KEGGivg:4811513.

Phylogenomic databases

KOiK10807.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of a very virulent Marek's disease virus."
    Tulman E.R., Afonso C.L., Lu Z., Zsak L., Rock D.L., Kutish G.F.
    J. Virol. 74:7980-7988(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiRIR1_GAHVM
AccessioniPrimary (citable) accession number: Q77MS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: October 13, 2009
Last modified: April 1, 2015
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.