Q77IX1 (NEP_I18A0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear export protein
Short name=NEP

Alternative name(s):
Non-structural protein 2
Short name=NS2

Gene names

Name:

NS

Organism

Influenza A virus (strain A/Brevig Mission/1/1918 H1N1) (Influenza A virus (strain A/South Carolina/1/1918 H1N1)) [Complete proteome]

Taxonomic identifier

88776 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A › Influenza A virus › H1N1 subtype

Virus host

Aves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	121 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Mediates the nuclear export of encapsidated genomic RNAs (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs complexes and the nuclear export machinery of the cell. Possesses no intrinsic RNA-binding activity, but includes a C-terminal M1-binding domain. This domain is believed to allow recognition of RNPs to which the M1 protein is bound. Because the M1 protein is not available in large quantities until the later stages of infection, such an indirect recognition mechanism probably ensures that genomic RNPs are not exported from the nucleus before sufficient quantities of viral mRNA and progeny genomic RNA have been synthesized. Furthermore, the RNPs enters the cytoplasm only when they have associated with the M1 protein that is necessary to guide them to the plasma membrane. May down-regulate viral RNA synthesis when overproduced By similarity.
Subunit structure	Binds M1 protein. May interact with human nucleoporin RAB/HRB and exportin XPO1/CRM1 By similarity.
Subcellular location	Virion Potential. Host nucleus By similarity.
Miscellaneous	Average number present in a viral particle is estimated to be 130-200 molecules. South Carolina isolate has been sequenced from formalid fixed-lung tissues of a 21-year-old male which died in 1918 at Ft. Jackson, SC. Brevig Mission isolate has been sequenced from lung tissues of an Inuit woman buried in the permafrost in a gravesite near Brevig Mission, Alaska. This sample was recovered by John Hultin, retired pathologist.
Sequence similarities	Belongs to the influenza viruses NEP family.

Ontologies

Keywords
Biological process	Host-virus interaction Transport
Cellular component	Host nucleus Virion
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA export from nucleus Inferred from electronic annotation. Source: InterPro virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform NEP (identifier: Q77IX1-1)

Also known as: NS2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform NS1 (identifier: Q99AU3-1)

The sequence of this isoform can be found in the external entry Q99AU3.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 121	121	Nuclear export protein		PRO_0000310568
Regions
	Motif	12 – 21	10	Nuclear export signal By similarity
	Motif	85 – 94	10	Nuclear export signal By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform NEP (NS2) [UniParc]. Last modified July 5, 2004. Version 1. Checksum: E89C109AE56E6AAF	FASTA	121	14,365
	10 20 30 40 50 60 MDSNTVSSFQ DILMRMSKMQ LGSSSEDLNG MITQFESLKL YRDSLGEAVM RMGDLHSLQN 70 80 90 100 110 120 RNGKWREQLS QKFEEIRWLI EEVRHRLKIT ENSFEQITFM QALQLLLEVE QEIRTFSFQL I
	Isoform NS1 [UniParc]. See Q99AU3.		–	–

References

[1]

"Sequence of the 1918 pandemic influenza virus nonstructural gene (NS) segment and characterization of recombinant viruses bearing the 1918 NS genes." Basler C.F., Reid A.H., Dybing J.K., Janczewski T.A., Fanning T.G., Zheng H., Salvatore M., Perdue M.L., Swayne D.E., Garcia-Sastre A., Palese P., Taubenberger J.K. Proc. Natl. Acad. Sci. U.S.A. 98:2746-2751(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF333238 Genomic RNA. Translation: AAK14369.1.
3D structure databases
HSSP	HSSP built from PDB template 1PD3 based on UniProtKB P03508.
ProteinModelPortal	Q77IX1.
SMR	Q77IX1. Positions 63-116.
ModBase	Search...
Proteomic databases
PRIDE	Q77IX1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000968. Flu_NS2. [Graphical view]
Pfam	PF00601. Flu_NS2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NEP_I18A0

Accession

Primary (citable) accession number: Q77IX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program