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Q77IX1 (NEP_I18A0) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear export protein

Short name=NEP
Alternative name(s):
Non-structural protein 2
Short name=NS2
Gene names
Name:NS
OrganismInfluenza A virus (strain A/Brevig Mission/1/1918 H1N1) (Influenza A virus (strain A/South Carolina/1/1918 H1N1)) [Complete proteome]
Taxonomic identifier88776 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length121 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Mediates the nuclear export of encapsidated genomic RNAs (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs complexes and the nuclear export machinery of the cell. Possesses no intrinsic RNA-binding activity, but includes a C-terminal M1-binding domain. This domain is believed to allow recognition of RNPs to which the M1 protein is bound. Because the M1 protein is not available in large quantities until the later stages of infection, such an indirect recognition mechanism probably ensures that genomic RNPs are not exported from the nucleus before sufficient quantities of viral mRNA and progeny genomic RNA have been synthesized. Furthermore, the RNPs enters the cytoplasm only when they have associated with the M1 protein that is necessary to guide them to the plasma membrane. May down-regulate viral RNA synthesis when overproduced By similarity.

Subunit structure

Binds M1 protein. May interact with human nucleoporin RAB/HRB and exportin XPO1/CRM1 By similarity.

Subcellular location

Virion Potential. Host nucleus By similarity.

Miscellaneous

Average number present in a viral particle is estimated to be 130-200 molecules.

South Carolina isolate has been sequenced from formalid fixed-lung tissues of a 21-year-old male which died in 1918 at Ft. Jackson, SC. Brevig Mission isolate has been sequenced from lung tissues of an Inuit woman buried in the permafrost in a gravesite near Brevig Mission, Alaska. This sample was recovered by John Hultin, retired pathologist.

Sequence similarities

Belongs to the influenza viruses NEP family.

Ontologies

Keywords
   Biological processHost-virus interaction
Transport
   Cellular componentHost nucleus
Virion
   Coding sequence diversityAlternative splicing
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processRNA export from nucleus

Inferred from electronic annotation. Source: InterPro

viral process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componenthost cell nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

virion

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform NEP (identifier: Q77IX1-1)

Also known as: NS2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform NS1 (identifier: Q99AU3-1)

The sequence of this isoform can be found in the external entry Q99AU3.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 121121Nuclear export protein
PRO_0000310568

Regions

Motif12 – 2110Nuclear export signal By similarity
Motif85 – 9410Nuclear export signal By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform NEP (NS2) [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: E89C109AE56E6AAF

FASTA12114,365
        10         20         30         40         50         60 
MDSNTVSSFQ DILMRMSKMQ LGSSSEDLNG MITQFESLKL YRDSLGEAVM RMGDLHSLQN 

        70         80         90        100        110        120 
RNGKWREQLS QKFEEIRWLI EEVRHRLKIT ENSFEQITFM QALQLLLEVE QEIRTFSFQL 


I 

« Hide

Isoform NS1 [UniParc].

See Q99AU3.

References

[1]"Sequence of the 1918 pandemic influenza virus nonstructural gene (NS) segment and characterization of recombinant viruses bearing the 1918 NS genes."
Basler C.F., Reid A.H., Dybing J.K., Janczewski T.A., Fanning T.G., Zheng H., Salvatore M., Perdue M.L., Swayne D.E., Garcia-Sastre A., Palese P., Taubenberger J.K.
Proc. Natl. Acad. Sci. U.S.A. 98:2746-2751(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF333238 Genomic RNA. Translation: AAK14369.1.

3D structure databases

ProteinModelPortalQ77IX1.
SMRQ77IX1. Positions 63-116.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ77IX1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000968. Flu_NS2.
[Graphical view]
PfamPF00601. Flu_NS2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNEP_I18A0
AccessionPrimary (citable) accession number: Q77IX1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: July 5, 2004
Last modified: February 19, 2014
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families