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Q77E03 (NCAP_VSIVN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Nucleoprotein
Short name=NP
Alternative name(s):
Nucleocapsid protein
Short name=Protein N
Gene names
Name:N
OrganismVesicular stomatitis Indiana virus (strain 98COE North America) (VSIV) [Complete proteome]
Taxonomic identifier434488 [NCBI]
Taxonomic lineageViruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Dimarhabdovirus supergroup › Vesiculovirus ›
Virus hostAedes [TaxID: 7158]
Bos taurus (Bovine) [TaxID: 9913]
Culicoides [TaxID: 58271]
Equus asinus (Donkey) [TaxID: 9793]
Equus caballus (Horse) [TaxID: 9796]
Homo sapiens (Human) [TaxID: 9606]
Lutzomyia [TaxID: 252607]
Musca domestica (House fly) [TaxID: 7370]
Simuliidae (black flies) [TaxID: 7190]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length422 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Replication is dependent on intracellular concentration of newly synthesized N, termed N0, which corresponds to the protein not associated with RNA. In contrast, when associated with RNA, it is termed N. During replication, encapsidation by N0 is coupled to RNA synthesis and all replicative products are resistant to nucleases By similarity.

Subunit structure

Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. N in nucleocapsid binds the P protein and thereby positions the polymerase on the template. Interaction of N0 with the P protein prevents the uncontrolled aggregation of N0 By similarity.

Subcellular location

Virion. Host cytoplasm. Note: The nucleocapsid is synthesized in the cytoplasm, and is subsequently transported via microtubules to the cell periphery By similarity.

Sequence similarities

Belongs to the vesiculovirus nucleocapsid protein family.

Ontologies

Keywords
   Cellular componentHost cytoplasm
Virion
   LigandRNA-binding
Viral nucleoprotein
   Molecular functionRibonucleoprotein
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Cellular_componenthost cell cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

ribonucleoprotein complex

Inferred from electronic annotation. Source: UniProtKB-KW

viral nucleocapsid

Inferred from electronic annotation. Source: InterPro

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 422422Nucleoprotein
PRO_0000287270

Secondary structure

1 .......................................................................... 422
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q77E03 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 7345C01BAB3C710E

FASTA42247,409
        10         20         30         40         50         60 
MSVTVKRIID NTVIVPKLPA NEDPVEYPAD YFRKSKEIPL YINTTKSLSD LRGYVYQGLK 

        70         80         90        100        110        120 
SGNVSIIHVN SYLYGALKDI RGKLDKDWSS FGINIGKAGD TIGIFDLVSL KALDGVLPDG 

       130        140        150        160        170        180 
VSDASRTSAD DKWLPLYLLG LYRVGRTQMP EYRKKLMDGL TNQCKMINEQ FEPLVPEGRD 

       190        200        210        220        230        240 
IFDVWGNDSN YTKIVAAVDM FFHMFKKHEC ASFRYGTIVS RFKDCAALAT FGHLCKITGM 

       250        260        270        280        290        300 
STEDVTTWIL NREVADEMVQ MMLPGQEIDK ADSYMPYLID FGLSSKSPYS SVKNPAFHFW 

       310        320        330        340        350        360 
GQLTALLLRS TRARNARQPD DIEYTSLTTA GLLYAYAVGS SADLAQQFCV GDNKYTPDDS 

       370        380        390        400        410        420 
TGGLTTNAPP QGRDVVEWLG WFEDQNRKPT PDMMQYAKRA VMSLQGLREK TIGKYAKSEF 


DK

« Hide

References

[1]"Full-length genome analysis of natural isolates of vesicular stomatitis virus (Indiana 1 serotype) from North, Central and South America."
Rodriguez L.L., Pauszek S.J., Bunch T.A., Schumann K.R.
J. Gen. Virol. 83:2475-2483(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF473864 Genomic RNA. Translation: AAN16980.1.
RefSeqNP_041712.1. NC_001560.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3HHWX-ray2.70K/L/M/N/O2-422[»]
3HHZX-ray3.50K/L/M/N/O2-422[»]
ProteinModelPortalQ77E03.
SMRQ77E03. Positions 2-422.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1489831.

Family and domain databases

Gene3D1.10.3570.10. 1 hit.
1.10.3610.10. 1 hit.
InterProIPR000448. Rhabdo_ncapsid.
IPR023331. Rhabdovirus_ncapsid_C.
IPR023330. Rhabdovirus_ncapsid_N.
[Graphical view]
PfamPF00945. Rhabdo_ncap. 1 hit.
[Graphical view]
ProDomPD002087. Rhabd_nucleocap. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF140809. SSF140809. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ77E03.

Entry information

Entry nameNCAP_VSIVN
AccessionPrimary (citable) accession number: Q77E03
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: July 5, 2004
Last modified: April 3, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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