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Q77DJ6

- VP40_EBOZ5

UniProt

Q77DJ6 - VP40_EBOZ5

Protein

Matrix protein VP40

Gene

VP40

Organism
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Promotes virus assembly and budding by interacting with host proteins of the multivesicular body pathway. May facilitate virus budding by interacting with the nucleocapsid and the plasma membrane. Specific interactions with membrane-associated GP and VP24 during the budding process may also occur. The hexamer form seems to be involved in budding. The octamer form binds RNA, and may play a role in genome replication By similarity.By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW
    2. structural constituent of virion Source: UniProtKB-KW

    GO - Biological processi

    1. viral budding via host ESCRT complex Source: UniProtKB-KW
    2. viral release from host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Biological processi

    Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral RNA replication, Virus exit from host cell

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Matrix protein VP40
    Alternative name(s):
    Membrane-associated protein VP40
    Gene namesi
    Name:VP40
    OrganismiZaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
    Taxonomic identifieri128951 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
    Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
    Homo sapiens (Human) [TaxID: 9606]
    Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
    ProteomesiUP000007208: Genome

    Subcellular locationi

    Virion membrane By similarity; Peripheral membrane protein By similarity. Host endomembrane system By similarity; Peripheral membrane protein By similarity. Host cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
    Note: In virion, localizes on the intravirional side of the membrane. In the host cell, it is found associated with virus-induced membrane proliferation foci and probably also in multivesicular bodies. These VP40-enriched membrane clusters are then redistributed to the plasma membrane where budding takes place By similarity.By similarity

    GO - Cellular componenti

    1. host cell endomembrane system Source: UniProtKB-SubCell
    2. host cell plasma membrane Source: UniProtKB-SubCell
    3. ribonucleoprotein complex Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 326326Matrix protein VP40PRO_0000245083Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer, homo-hexamer or homo-octamer. Membrane association induces conformational switch from monomer to hexamer. Interacts with host TSG101. Homo-hexamer interacts with the WW domain 3 of host NEDD4. Interacts with the nucleoprotein. May interact with VP35 By similarity.By similarity

    Structurei

    Secondary structure

    1
    326
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi61 – 633
    Beta strandi71 – 8313
    Beta strandi86 – 10116
    Beta strandi103 – 1064
    Helixi108 – 1169
    Beta strandi120 – 1256
    Beta strandi128 – 1303
    Beta strandi132 – 1376
    Helixi148 – 1525
    Beta strandi153 – 1586
    Helixi159 – 1624
    Beta strandi173 – 18513
    Beta strandi203 – 2086
    Helixi228 – 2314
    Helixi234 – 24310
    Helixi244 – 2463
    Beta strandi248 – 2536
    Helixi254 – 2563
    Beta strandi258 – 2625
    Helixi265 – 2717
    Beta strandi284 – 2885
    Turni298 – 3014
    Beta strandi304 – 3107

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ES6X-ray2.00A31-326[»]
    ProteinModelPortaliQ77DJ6.
    SMRiQ77DJ6. Positions 44-321.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ77DJ6.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni212 – 2143Important for oligomerizationBy similarity
    Regioni213 – 326114Membrane-bindingAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi7 – 104PTAP/PSAP motif
    Motifi10 – 134PPXY motif

    Domaini

    Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. VP40 contains two overlapping L domains: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 and a PPXY motif which interacts with the WW domain 3 of NEDD4.

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR008986. EV_matrix.
    [Graphical view]
    PfamiPF07447. VP40. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018327. VP40_FiloV. 1 hit.
    SUPFAMiSSF50012. SSF50012. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q77DJ6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRRVILPTAP PEYMEAIYPV RSNSTIARGG NSNTGFLTPE SVNGDTPSNP    50
    LRPIADDTID HASHTPGSVS SAFILEAMVN VISGPKVLMK QIPIWLPLGV 100
    ADQKTYSFDS TTAAIMLASY TITHFGKATN PLVRVNRLGP GIPDHPLRLL 150
    RIGNQAFLQE FVLPPVQLPQ YFTFDLTALK LITQPLPAAT WTDDTPTGSN 200
    GALRPGISFH PKLRPILLPN KSGKKGNSAD LTSPEKIQAI MTSLQDFKIV 250
    PIDPTKNIMG IEVPETLVHK LTGKKVTSKN GQPIIPVLLP KYIGLDPVAP 300
    GDLTMVITQD CDTCHSPASL PAVIEK 326
    Length:326
    Mass (Da):35,183
    Last modified:July 5, 2004 - v1
    Checksum:i1AB132C0DB8E9003
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY354458 Genomic RNA. Translation: AAQ55047.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY354458 Genomic RNA. Translation: AAQ55047.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ES6 X-ray 2.00 A 31-326 [» ]
    ProteinModelPortali Q77DJ6.
    SMRi Q77DJ6. Positions 44-321.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q77DJ6.

    Family and domain databases

    InterProi IPR008986. EV_matrix.
    [Graphical view ]
    Pfami PF07447. VP40. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018327. VP40_FiloV. 1 hit.
    SUPFAMi SSF50012. SSF50012. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Crystal structure of the matrix protein VP40 from Ebola virus."
      Dessen A., Volchkov V., Dolnik O., Klenk H.-D., Weissenhorn W.
      EMBO J. 19:4228-4236(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 31-326.

    Entry informationi

    Entry nameiVP40_EBOZ5
    AccessioniPrimary (citable) accession number: Q77DJ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Most abundant protein in the virion.By similarity

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3