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Q77DJ6 (VP40_EBOZ5) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Matrix protein VP40
Alternative name(s):
Membrane-associated protein VP40
Gene names
Name:VP40
OrganismZaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus) [Complete proteome]
Taxonomic identifier128951 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes virus assembly and budding by interacting with host proteins of the multivesicular body pathway. May facilitate virus budding by interacting with the nucleocapsid and the plasma membrane. Specific interactions with membrane-associated GP and VP24 during the budding process may also occur. The hexamer form seems to be involved in budding. The octamer form binds RNA, and may play a role in genome replication By similarity.

Subunit structure

Monomer, homo-hexamer or homo-octamer. Membrane association induces conformational switch from monomer to hexamer. Interacts with host TSG101. Homo-hexamer interacts with the WW domain 3 of host NEDD4. Interacts with the nucleoprotein. May interact with VP35 By similarity.

Subcellular location

Virion membrane; Peripheral membrane protein By similarity. Host endomembrane system; Peripheral membrane protein By similarity. Host cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: In virion, localizes on the intravirional side of the membrane. In the host cell, it is found associated with virus-induced membrane proliferation foci and probably also in multivesicular bodies. These VP40-enriched membrane clusters are then redistributed to the plasma membrane where budding takes place By similarity.

Domain

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. VP40 contains two overlapping L domains: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 and a PPXY motif which interacts with the WW domain 3 of NEDD4.

Miscellaneous

Most abundant protein in the virion By similarity.

Sequence similarities

Belongs to the filoviridae matrix protein VP40 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 326326Matrix protein VP40
PRO_0000245083

Regions

Region212 – 2143Important for oligomerization By similarity
Region213 – 326114Membrane-binding
Motif7 – 104PTAP/PSAP motif
Motif10 – 134PPXY motif

Secondary structure

........................................... 326
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q77DJ6 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 1AB132C0DB8E9003

FASTA32635,183
        10         20         30         40         50         60 
MRRVILPTAP PEYMEAIYPV RSNSTIARGG NSNTGFLTPE SVNGDTPSNP LRPIADDTID 

        70         80         90        100        110        120 
HASHTPGSVS SAFILEAMVN VISGPKVLMK QIPIWLPLGV ADQKTYSFDS TTAAIMLASY 

       130        140        150        160        170        180 
TITHFGKATN PLVRVNRLGP GIPDHPLRLL RIGNQAFLQE FVLPPVQLPQ YFTFDLTALK 

       190        200        210        220        230        240 
LITQPLPAAT WTDDTPTGSN GALRPGISFH PKLRPILLPN KSGKKGNSAD LTSPEKIQAI 

       250        260        270        280        290        300 
MTSLQDFKIV PIDPTKNIMG IEVPETLVHK LTGKKVTSKN GQPIIPVLLP KYIGLDPVAP 

       310        320 
GDLTMVITQD CDTCHSPASL PAVIEK 

« Hide

References

[1]Chain P.S.G., Ichou M.A., Malfatti S.A., Hajjaj A., Vergez L.M., Paragas J., Do L.H., Jahrling P.B., Smith K.L., McCready P.M., Ibrahim M.S.
Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Crystal structure of the matrix protein VP40 from Ebola virus."
Dessen A., Volchkov V., Dolnik O., Klenk H.-D., Weissenhorn W.
EMBO J. 19:4228-4236(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 31-326.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY354458 Genomic RNA. Translation: AAQ55047.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ES6X-ray2.00A31-326[»]
ProteinModelPortalQ77DJ6.
SMRQ77DJ6. Positions 44-321.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR008986. EV_matrix.
[Graphical view]
PfamPF07447. VP40. 1 hit.
[Graphical view]
PIRSFPIRSF018327. VP40_FiloV. 1 hit.
SUPFAMSSF50012. SSF50012. 2 hits.
ProtoNetSearch...

Other

EvolutionaryTraceQ77DJ6.

Entry information

Entry nameVP40_EBOZ5
AccessionPrimary (citable) accession number: Q77DJ6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 5, 2004
Last modified: February 19, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references