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Q77DJ6

- VP40_EBOZ5

UniProt

Q77DJ6 - VP40_EBOZ5

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Protein

Matrix protein VP40

Gene

VP40

Organism
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Promotes virus assembly and budding by interacting with host proteins of the multivesicular body pathway. May facilitate virus budding by interacting with the nucleocapsid and the plasma membrane. Specific interactions with membrane-associated GP and VP24 during the budding process may also occur. The hexamer form seems to be involved in budding. The octamer form binds RNA, and may play a role in genome replication (By similarity).By similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural constituent of virion Source: UniProtKB-KW

GO - Biological processi

  1. viral budding via host ESCRT complex Source: UniProtKB-KW
  2. viral release from host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral RNA replication, Virus exit from host cell

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix protein VP40
Alternative name(s):
Membrane-associated protein VP40
Gene namesi
Name:VP40
OrganismiZaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
Taxonomic identifieri128951 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
ProteomesiUP000007208: Genome

Subcellular locationi

Virion membrane By similarity; Peripheral membrane protein By similarity. Host endomembrane system By similarity; Peripheral membrane protein By similarity. Host cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Note: In virion, localizes on the intravirional side of the membrane. In the host cell, it is found associated with virus-induced membrane proliferation foci and probably also in multivesicular bodies. These VP40-enriched membrane clusters are then redistributed to the plasma membrane where budding takes place (By similarity).By similarity

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
  3. ribonucleoprotein complex Source: UniProtKB-KW
  4. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 326326Matrix protein VP40PRO_0000245083Add
BLAST

Interactioni

Subunit structurei

Monomer, homo-hexamer or homo-octamer. Membrane association induces conformational switch from monomer to hexamer. Interacts with host TSG101. Homo-hexamer interacts with the WW domain 3 of host NEDD4. Interacts with the nucleoprotein. May interact with VP35 (By similarity).By similarity

Structurei

Secondary structure

1
326
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi61 – 633Combined sources
Beta strandi71 – 8313Combined sources
Beta strandi86 – 10116Combined sources
Beta strandi103 – 1064Combined sources
Helixi108 – 1169Combined sources
Beta strandi120 – 1256Combined sources
Beta strandi128 – 1303Combined sources
Beta strandi132 – 1376Combined sources
Helixi148 – 1525Combined sources
Beta strandi153 – 1586Combined sources
Helixi159 – 1624Combined sources
Beta strandi173 – 18513Combined sources
Beta strandi203 – 2086Combined sources
Helixi228 – 2314Combined sources
Helixi234 – 24310Combined sources
Helixi244 – 2463Combined sources
Beta strandi248 – 2536Combined sources
Helixi254 – 2563Combined sources
Beta strandi258 – 2625Combined sources
Helixi265 – 2717Combined sources
Beta strandi284 – 2885Combined sources
Turni298 – 3014Combined sources
Beta strandi304 – 3107Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ES6X-ray2.00A31-326[»]
ProteinModelPortaliQ77DJ6.
SMRiQ77DJ6. Positions 44-321.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ77DJ6.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni212 – 2143Important for oligomerizationBy similarity
Regioni213 – 326114Membrane-bindingAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi7 – 104PTAP/PSAP motif
Motifi10 – 134PPXY motif

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. VP40 contains two overlapping L domains: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 and a PPXY motif which interacts with the WW domain 3 of NEDD4.

Sequence similaritiesi

Family and domain databases

InterProiIPR008986. EV_matrix.
[Graphical view]
PfamiPF07447. VP40. 1 hit.
[Graphical view]
PIRSFiPIRSF018327. VP40_FiloV. 1 hit.
SUPFAMiSSF50012. SSF50012. 2 hits.

Sequencei

Sequence statusi: Complete.

Q77DJ6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRRVILPTAP PEYMEAIYPV RSNSTIARGG NSNTGFLTPE SVNGDTPSNP
60 70 80 90 100
LRPIADDTID HASHTPGSVS SAFILEAMVN VISGPKVLMK QIPIWLPLGV
110 120 130 140 150
ADQKTYSFDS TTAAIMLASY TITHFGKATN PLVRVNRLGP GIPDHPLRLL
160 170 180 190 200
RIGNQAFLQE FVLPPVQLPQ YFTFDLTALK LITQPLPAAT WTDDTPTGSN
210 220 230 240 250
GALRPGISFH PKLRPILLPN KSGKKGNSAD LTSPEKIQAI MTSLQDFKIV
260 270 280 290 300
PIDPTKNIMG IEVPETLVHK LTGKKVTSKN GQPIIPVLLP KYIGLDPVAP
310 320
GDLTMVITQD CDTCHSPASL PAVIEK
Length:326
Mass (Da):35,183
Last modified:July 5, 2004 - v1
Checksum:i1AB132C0DB8E9003
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY354458 Genomic RNA. Translation: AAQ55047.1.
RefSeqiNP_066245.1. NC_002549.1.

Genome annotation databases

GeneIDi911825.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY354458 Genomic RNA. Translation: AAQ55047.1 .
RefSeqi NP_066245.1. NC_002549.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ES6 X-ray 2.00 A 31-326 [» ]
ProteinModelPortali Q77DJ6.
SMRi Q77DJ6. Positions 44-321.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 911825.

Miscellaneous databases

EvolutionaryTracei Q77DJ6.

Family and domain databases

InterProi IPR008986. EV_matrix.
[Graphical view ]
Pfami PF07447. VP40. 1 hit.
[Graphical view ]
PIRSFi PIRSF018327. VP40_FiloV. 1 hit.
SUPFAMi SSF50012. SSF50012. 2 hits.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Crystal structure of the matrix protein VP40 from Ebola virus."
    Dessen A., Volchkov V., Dolnik O., Klenk H.-D., Weissenhorn W.
    EMBO J. 19:4228-4236(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 31-326.

Entry informationi

Entry nameiVP40_EBOZ5
AccessioniPrimary (citable) accession number: Q77DJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 5, 2004
Last modified: November 26, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Most abundant protein in the virion.By similarity

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3