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Q77DJ5

- VP30_EBOZ5

UniProt

Q77DJ5 - VP30_EBOZ5

Protein

Minor nucleoprotein VP30

Gene

VP30

Organism
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA stem-loop at the transcription start site of the first gene. Binds to RNA By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri72 – 9019C3H1-type; atypicalAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription

    Keywords - Ligandi

    Metal-binding, RNA-binding, Viral nucleoprotein, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Minor nucleoprotein VP30
    Alternative name(s):
    Transcription activator VP30
    Gene namesi
    Name:VP30
    OrganismiZaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
    Taxonomic identifieri128951 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
    Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
    Homo sapiens (Human) [TaxID: 9606]
    Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
    ProteomesiUP000007208: Genome

    Subcellular locationi

    Virion. Host cytoplasm By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. viral nucleocapsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Host cytoplasm, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 288288Minor nucleoprotein VP30PRO_0000245074Add
    BLAST

    Post-translational modificationi

    Phosphorylated by host. Phosphorylation negatively regulates the transcription activation By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Homohexamer or homodimer. Interacts with the nucleoprotein.

    Protein-protein interaction databases

    DIPiDIP-60797N.

    Structurei

    Secondary structure

    1
    288
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi144 – 15512
    Helixi164 – 18017
    Helixi183 – 19412
    Helixi201 – 2033
    Helixi204 – 21512
    Beta strandi218 – 2203
    Helixi221 – 2299
    Helixi232 – 24716
    Beta strandi250 – 2523
    Helixi255 – 2628

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2I8BX-ray2.00A/B142-272[»]
    ProteinModelPortaliQ77DJ5.
    SMRiQ77DJ5. Positions 142-266.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ77DJ5.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 4444Intrinsically disorderedSequence AnalysisAdd
    BLAST
    Regioni26 – 4015RNA-bindingBy similarityAdd
    BLAST
    Regioni94 – 11219OligomerizationAdd
    BLAST
    Regioni121 – 14020Intrinsically disorderedSequence AnalysisAdd
    BLAST
    Regioni180 – 19718Interaction with the nucleoproteinAdd
    BLAST
    Regioni268 – 28821Intrinsically disorderedSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C3H1-type zinc finger.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri72 – 9019C3H1-type; atypicalAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Family and domain databases

    InterProiIPR014459. VP30_FiloV.
    [Graphical view]
    PfamiPF11507. Transcript_VP30. 1 hit.
    [Graphical view]
    PIRSFiPIRSF011356. VP30_FiloV. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q77DJ5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEASYERGRP RAARQHSRDG HDHHVRARSS SRENYRGEYR QSRSASQVRV    50
    PTVFHKKRVE PLTVPPAPKD ICPTLKKGFL CDSSFCKKDH QLESLTDREL 100
    LLLIARKTCG SVEQQLNITA PKDSRLANPT ADDFQQEEGP KITLLTLIKT 150
    AEHWARQDIR TIEDSKLRAL LTLCAVMTRK FSKSQLSLLC ETHLRREGLG 200
    QDQAEPVLEV YQRLHSDKGG SFEAALWQQW DRQSLIMFIT AFLNIALQLP 250
    CESSAVVVSG LRTLVPQSDN EEASTNPGTC SWSDEGTP 288
    Length:288
    Mass (Da):32,521
    Last modified:July 5, 2004 - v1
    Checksum:i1FE40E93AB80454B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY354458 Genomic RNA. Translation: AAQ55051.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY354458 Genomic RNA. Translation: AAQ55051.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2I8B X-ray 2.00 A/B 142-272 [» ]
    ProteinModelPortali Q77DJ5.
    SMRi Q77DJ5. Positions 142-266.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60797N.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q77DJ5.

    Family and domain databases

    InterProi IPR014459. VP30_FiloV.
    [Graphical view ]
    Pfami PF11507. Transcript_VP30. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF011356. VP30_FiloV. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Crystal structure of the C-terminal domain of Ebola virus VP30 reveals a role in transcription and nucleocapsid association."
      Hartlieb B., Muziol T., Weissenhorn W., Becker S.
      Proc. Natl. Acad. Sci. U.S.A. 104:624-629(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 142-272.

    Entry informationi

    Entry nameiVP30_EBOZ5
    AccessioniPrimary (citable) accession number: Q77DJ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3