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Protein

Minor nucleoprotein VP30

Gene

VP30

Organism
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA stem-loop at the transcription start site of the first gene. Binds to RNA (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri72 – 90C3H1-type; atypicalAdd BLAST19

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription

Keywords - Ligandi

Metal-binding, RNA-binding, Viral nucleoprotein, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Minor nucleoprotein VP30
Alternative name(s):
Transcription activator VP30
Gene namesi
Name:VP30
OrganismiZaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
Taxonomic identifieri128951 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
Proteomesi
  • UP000007208 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002450741 – 288Minor nucleoprotein VP30Add BLAST288

Post-translational modificationi

Phosphorylated by host. Phosphorylation negatively regulates the transcription activation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homohexamer or homodimer. Interacts with the nucleoprotein.

Protein-protein interaction databases

DIPiDIP-60797N.

Structurei

Secondary structure

1288
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi144 – 155Combined sources12
Helixi164 – 180Combined sources17
Helixi183 – 194Combined sources12
Helixi201 – 203Combined sources3
Helixi204 – 215Combined sources12
Beta strandi218 – 220Combined sources3
Helixi221 – 229Combined sources9
Helixi232 – 247Combined sources16
Beta strandi250 – 252Combined sources3
Helixi255 – 262Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I8BX-ray2.00A/B142-272[»]
ProteinModelPortaliQ77DJ5.
SMRiQ77DJ5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ77DJ5.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 44Intrinsically disorderedSequence analysisAdd BLAST44
Regioni26 – 40RNA-bindingBy similarityAdd BLAST15
Regioni94 – 112OligomerizationAdd BLAST19
Regioni121 – 140Intrinsically disorderedSequence analysisAdd BLAST20
Regioni180 – 197Interaction with the nucleoproteinAdd BLAST18
Regioni268 – 288Intrinsically disorderedSequence analysisAdd BLAST21

Sequence similaritiesi

Contains 1 C3H1-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri72 – 90C3H1-type; atypicalAdd BLAST19

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR014459. VP30_FiloV.
[Graphical view]
PfamiPF11507. Transcript_VP30. 1 hit.
[Graphical view]
PIRSFiPIRSF011356. VP30_FiloV. 1 hit.

Sequencei

Sequence statusi: Complete.

Q77DJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEASYERGRP RAARQHSRDG HDHHVRARSS SRENYRGEYR QSRSASQVRV
60 70 80 90 100
PTVFHKKRVE PLTVPPAPKD ICPTLKKGFL CDSSFCKKDH QLESLTDREL
110 120 130 140 150
LLLIARKTCG SVEQQLNITA PKDSRLANPT ADDFQQEEGP KITLLTLIKT
160 170 180 190 200
AEHWARQDIR TIEDSKLRAL LTLCAVMTRK FSKSQLSLLC ETHLRREGLG
210 220 230 240 250
QDQAEPVLEV YQRLHSDKGG SFEAALWQQW DRQSLIMFIT AFLNIALQLP
260 270 280
CESSAVVVSG LRTLVPQSDN EEASTNPGTC SWSDEGTP
Length:288
Mass (Da):32,521
Last modified:July 5, 2004 - v1
Checksum:i1FE40E93AB80454B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY354458 Genomic RNA. Translation: AAQ55051.1.
RefSeqiNP_066249.1. NC_002549.1.

Genome annotation databases

GeneIDi911826.
KEGGivg:911826.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY354458 Genomic RNA. Translation: AAQ55051.1.
RefSeqiNP_066249.1. NC_002549.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I8BX-ray2.00A/B142-272[»]
ProteinModelPortaliQ77DJ5.
SMRiQ77DJ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60797N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi911826.
KEGGivg:911826.

Miscellaneous databases

EvolutionaryTraceiQ77DJ5.

Family and domain databases

InterProiIPR014459. VP30_FiloV.
[Graphical view]
PfamiPF11507. Transcript_VP30. 1 hit.
[Graphical view]
PIRSFiPIRSF011356. VP30_FiloV. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiVP30_EBOZ5
AccessioniPrimary (citable) accession number: Q77DJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.