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Q77DJ5 (VP30_EBOZ5) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Minor nucleoprotein VP30
Alternative name(s):
Transcription activator VP30
Gene names
Name:VP30
OrganismZaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus) [Complete proteome]
Taxonomic identifier128951 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]

Protein attributes

Sequence length288 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA stem-loop at the transcription start site of the first gene. Binds to RNA By similarity.

Subunit structure

Homohexamer or homodimer. Interacts with the nucleoprotein.

Subcellular location

Virion. Host cytoplasm By similarity.

Post-translational modification

Phosphorylated by host. Phosphorylation negatively regulates the transcription activation By similarity.

Sequence similarities

Belongs to the filoviridae minor nucleoprotein VP30 family.

Contains 1 C3H1-type zinc finger.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentHost cytoplasm
Virion
   DomainZinc-finger
   LigandMetal-binding
RNA-binding
Viral nucleoprotein
Zinc
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological_processtranscription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componenthost cell cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

viral nucleocapsid

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 288288Minor nucleoprotein VP30
PRO_0000245074

Regions

Zinc finger72 – 9019C3H1-type; atypical
Region1 – 4444Intrinsically disordered Potential
Region26 – 4015RNA-binding By similarity
Region94 – 11219Oligomerization
Region121 – 14020Intrinsically disordered Potential
Region180 – 19718Interaction with the nucleoprotein
Region268 – 28821Intrinsically disordered Potential

Secondary structure

................... 288
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q77DJ5 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 1FE40E93AB80454B

FASTA28832,521
        10         20         30         40         50         60 
MEASYERGRP RAARQHSRDG HDHHVRARSS SRENYRGEYR QSRSASQVRV PTVFHKKRVE 

        70         80         90        100        110        120 
PLTVPPAPKD ICPTLKKGFL CDSSFCKKDH QLESLTDREL LLLIARKTCG SVEQQLNITA 

       130        140        150        160        170        180 
PKDSRLANPT ADDFQQEEGP KITLLTLIKT AEHWARQDIR TIEDSKLRAL LTLCAVMTRK 

       190        200        210        220        230        240 
FSKSQLSLLC ETHLRREGLG QDQAEPVLEV YQRLHSDKGG SFEAALWQQW DRQSLIMFIT 

       250        260        270        280 
AFLNIALQLP CESSAVVVSG LRTLVPQSDN EEASTNPGTC SWSDEGTP 

« Hide

References

[1]Chain P.S.G., Ichou M.A., Malfatti S.A., Hajjaj A., Vergez L.M., Paragas J., Do L.H., Jahrling P.B., Smith K.L., McCready P.M., Ibrahim M.S.
Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Crystal structure of the C-terminal domain of Ebola virus VP30 reveals a role in transcription and nucleocapsid association."
Hartlieb B., Muziol T., Weissenhorn W., Becker S.
Proc. Natl. Acad. Sci. U.S.A. 104:624-629(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 142-272.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY354458 Genomic RNA. Translation: AAQ55051.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2I8BX-ray2.00A/B142-272[»]
ProteinModelPortalQ77DJ5.
SMRQ77DJ5. Positions 142-266.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60797N.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR014459. VP30_FiloV.
[Graphical view]
PfamPF11507. Transcript_VP30. 1 hit.
[Graphical view]
PIRSFPIRSF011356. VP30_FiloV. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ77DJ5.

Entry information

Entry nameVP30_EBOZ5
AccessionPrimary (citable) accession number: Q77DJ5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references