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Q77DJ5

- VP30_EBOZ5

UniProt

Q77DJ5 - VP30_EBOZ5

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Protein

Minor nucleoprotein VP30

Gene
VP30
Organism
Zaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as a transcription anti-termination factor immediately after transcription initiation, but does not affect transcription elongation. This function has been found to be dependent on the formation of an RNA stem-loop at the transcription start site of the first gene. Binds to RNA By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri72 – 9019C3H1-type; atypicalAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription

Keywords - Ligandi

Metal-binding, RNA-binding, Viral nucleoprotein, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Minor nucleoprotein VP30
Alternative name(s):
Transcription activator VP30
Gene namesi
Name:VP30
OrganismiZaire ebolavirus (strain Kikwit-95) (ZEBOV) (Zaire Ebola virus)
Taxonomic identifieri128951 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
ProteomesiUP000007208: Genome

Subcellular locationi

Virion. Host cytoplasm By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288Minor nucleoprotein VP30PRO_0000245074Add
BLAST

Post-translational modificationi

Phosphorylated by host. Phosphorylation negatively regulates the transcription activation By similarity.

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homohexamer or homodimer. Interacts with the nucleoprotein.

Protein-protein interaction databases

DIPiDIP-60797N.

Structurei

Secondary structure

1
288
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi144 – 15512
Helixi164 – 18017
Helixi183 – 19412
Helixi201 – 2033
Helixi204 – 21512
Beta strandi218 – 2203
Helixi221 – 2299
Helixi232 – 24716
Beta strandi250 – 2523
Helixi255 – 2628

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2I8BX-ray2.00A/B142-272[»]
ProteinModelPortaliQ77DJ5.
SMRiQ77DJ5. Positions 142-266.

Miscellaneous databases

EvolutionaryTraceiQ77DJ5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 4444Intrinsically disordered Reviewed predictionAdd
BLAST
Regioni26 – 4015RNA-binding By similarityAdd
BLAST
Regioni94 – 11219OligomerizationAdd
BLAST
Regioni121 – 14020Intrinsically disordered Reviewed predictionAdd
BLAST
Regioni180 – 19718Interaction with the nucleoproteinAdd
BLAST
Regioni268 – 28821Intrinsically disordered Reviewed predictionAdd
BLAST

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri72 – 9019C3H1-type; atypicalAdd
BLAST

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR014459. VP30_FiloV.
[Graphical view]
PfamiPF11507. Transcript_VP30. 1 hit.
[Graphical view]
PIRSFiPIRSF011356. VP30_FiloV. 1 hit.

Sequencei

Sequence statusi: Complete.

Q77DJ5-1 [UniParc]FASTAAdd to Basket

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MEASYERGRP RAARQHSRDG HDHHVRARSS SRENYRGEYR QSRSASQVRV    50
PTVFHKKRVE PLTVPPAPKD ICPTLKKGFL CDSSFCKKDH QLESLTDREL 100
LLLIARKTCG SVEQQLNITA PKDSRLANPT ADDFQQEEGP KITLLTLIKT 150
AEHWARQDIR TIEDSKLRAL LTLCAVMTRK FSKSQLSLLC ETHLRREGLG 200
QDQAEPVLEV YQRLHSDKGG SFEAALWQQW DRQSLIMFIT AFLNIALQLP 250
CESSAVVVSG LRTLVPQSDN EEASTNPGTC SWSDEGTP 288
Length:288
Mass (Da):32,521
Last modified:July 5, 2004 - v1
Checksum:i1FE40E93AB80454B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY354458 Genomic RNA. Translation: AAQ55051.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY354458 Genomic RNA. Translation: AAQ55051.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2I8B X-ray 2.00 A/B 142-272 [» ]
ProteinModelPortali Q77DJ5.
SMRi Q77DJ5. Positions 142-266.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-60797N.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q77DJ5.

Family and domain databases

InterProi IPR014459. VP30_FiloV.
[Graphical view ]
Pfami PF11507. Transcript_VP30. 1 hit.
[Graphical view ]
PIRSFi PIRSF011356. VP30_FiloV. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Crystal structure of the C-terminal domain of Ebola virus VP30 reveals a role in transcription and nucleocapsid association."
    Hartlieb B., Muziol T., Weissenhorn W., Becker S.
    Proc. Natl. Acad. Sci. U.S.A. 104:624-629(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 142-272.

Entry informationi

Entry nameiVP30_EBOZ5
AccessioniPrimary (citable) accession number: Q77DJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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